Lamc1 (NM_053966) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	B2e
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217665 representing NM_053966 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGGGCGGCAGGCGGGCCGCTCTGGCCCTGCAGCTCCAGCGGCGGCTGTGGCTGCTGCTGGCTGTGC TGGCGGCCGTGGCGTGCTGGGTCCGGGCGGCCATGGACGAGTGCGCGGATGAGGGCGGCCGGCCGCAGCG CTGCATGCCGGAGTTTGTTAATGCCGCCTTCAATGTGACCGTGGTGGCTACCAACACGTGTGGGACTCCA CCCGAGGAGTACTGCGTGCAGACTGGAGTGACCGGAGTCACCAAGTCCTGTCACCTGTGCGACGCCGGCC AGCTCCACCTGCAACACGGGGCAGCCTTCTTGACCGACTACAACAACCAGGCCGACACCACCTGGTGGCA AAGCCAGACTATGCTGGCCGGGGTGCAGTACCCCAACTCCATCAACCTCACGCTGCACCTGGGAAAGGCT TTTGACATCACTTACGTGCGTCTCAAGTTCCACACCAGCCGCCCAGAGAGTTTCGCCATTTATAAGCGCA CTCGGGAAGACGGGCCCTGGATTCCTTACCAGTACTACAGTGGCTCCTGTGAGAACACGTACTCAAAGGC TAACCGTGGCTTCATCAGGACGGGAGGAGATGAGCAGCAGGCCTTGTGTACCGATGAGTTCAGTGACATT TCCCCCCTCACCGGCGGCAACGTGGCCTTTTCAACTCTGGAAGGAAGGCCGAGTGCCTACAACTTTGACA ACAGCCCCGTGCTACAGGAATGGGTGACAGCCACTGACATCAGAGTCACACTCAATCGCCTGAACACCTT TGGAGATGAAGTATTCAATGACCCCAAAGTCCTCAAGTCTTACTATTATGCAATCTCGGACTTTGCTGTG GGTGGCAGGTGTAAATGTAATGGACATGCCAGTGAGTGTGTAAAGAACGAGTTTGGGAAGCTCGTGTGCA ACTGCAAGCATAACACATACGGAGTTGACTGTGAAAAGTGCCTGCCTTTCTTCAACGATCGGCCGTGGAG GAGAGCAACTGCCGAGAGTGCCAACCAGTGCCTGCCTTGCGACTGCAATGGCCGATCCCAAGAATGCTAC TTTGACCCTGAGCTATACCGTTCCACTGGGCACGGTGGCCACTGTACCAACTGCCGGGATAACACAGACG GTGCCAAGTGCGAGAGGTGCCGGGAGAACTTCTTCCGCCTGGGGAACACTGAAGCCTGCTCTCCGTGCCA CTGCAGCCCCGTTGGTTCTCTCAGCACGCAGTGTGACAGTTACGGCAGGTGCAGCTGTAAGCCCGGAGTG ATGGGTGACAAGTGTGACCGTTGCCAGCCTGGGTTCCATTCCCTCACCGAGGCAGGATGCAGGCCATGCT CCTGCGACCCTTCTGGCAGCACCGACGAATGTAATGTTGAAACAGGAAGATGCGTTTGCAAAGACAACGT CGAAGGCTTCAGCTGTGAGAGATGCAAACCTGGATTTTTTAATCTGGAATCCTCCAATCCTAAGGGTTGC ACACCCTGCTTCTGCTTTGGGCATTCTTCTGTGTGCACCAATGCTGTTGGCTACAGTGTTTATGACATCT CCTCGACCTTTCAGATTGATGAGGACGGGTGGCGTGTGGAGCAGAGAGATGGCTCGGAGGCGTCTCTTGA GTGGTCCTCAGACCGGCAGGATATTGCTGTGATCTCAGACACTTACTTCCCCAGATACTTTGTCGCCCCT GCCAAGTTCCTGGGCAACCAGGTCCTGAGTTACGGGCAGAACCTCTCATTCTCCTTCCGAGTGGACAGAC GGGACACTCGCCTCTCCGCAGAGGATCTCGTGCTGGAAGGAGCTGGCTTGAGAGTATCCGTGCCCTTGAT CGCTCAGGGCAACTCGTACCCTAGCGAGACCACTGTGAAGTACATCTTCAGACTCCATGAAGCAACAGAT TACCCTTGGAGGCCTGCTCTCTCCCCATTTGAATTTCAGAAGCTCCTAAACAACTTGACCTCTATCAAGA TCCGTGGTACATACAGTGAGAGGAGCGCTGGATATTTGGATGATGTCACCTTGCAAAGTGCTCGCCCTGG GCCCGGAGTCCCCGCAACGTGGGTGGAGTCCTGCACCTGTCCAGTGGGGTATGGAGGGCAGTTCTGTGAG ACATGCCTCCCAGGCTACAGAAGAGAAACCCCAAGTCTTGGACCTTACAGCCCGTGTGTGCTCTGTACCT GTAATGGGCACAGTGAGACCTGTGACCCCGAGACAGGTGTCTGTGACTGCAGAGACAATACTGCCGGCCC GCACTGTGAGAAATGTAGCGATGGGTACTATGGAGACTCAACCCTGGGCACCTCCTCTGACTGCCAGCCT TGTCCCTGCCCTGGCGGCTCAAGTTGTGCCATTGTCCCAAAGACAAAGGAAGTGGTGTGCACACACTGTC CAACTGGCACTGCCGGCAAGAGATGTGAGCTCTGTGATGACGGCTACTTTGGAGACCCTCTGGGCAGCAA TGGACCCGTGAGACTCTGCCGCCCATGTCAGTGCAACGACAACATAGACCCCAACGCAGTTGGCAACTGC AACCGTCTGACTGGAGAATGCCTGAAGTGCATCTATAACACGGCTGGCTTCTACTGCGACCGGTGCAAAG AAGGGTTTTTCGGAAATCCCCTGGCTCCCAATCCAGCAGACAAGTGCAAAGCCTGCGCCTGCAATCCCTA TGGTACAGTACAGCAGCAGAGCAGCTGTAACCCAGTGACCGGGCAGTGCCAGTGTCTGCCTCATGTGTCA GGCCGGGACTGTGGCGCTTGTGACCCTGGCTTCTACAACCTGCAGAGCGGGCAAGGCTGCGAGAGTTGTG ACTGCCATGCTTTGGGCTCCACCAACGGGCAATGTGACATCCGCACCGGGCAGTGTGAGTGTCAGCCCGG CATCACCGGTCAGCACTGTGAGCGCTGTGAGACCAACCACTTTGGGTTTGGACCTGAAGGCTGCAAACCT TGCGACTGTCACCACGAAGGATCCCTTTCGCTCCAATGTAAGGATGACGGCCGTTGTGAATGCAGGGAAG GCTTCGTGGGCAATCGCTGTGACCAGTGTGAAGAGAACTATTTCTACAATCGGTCTTGGCCTGGCTGCCA AGAGTGTCCGGCTTGTTACCGGCTGGTGAAGGATAAGGTTGCTGAGCATCGAGTGAAACTCCAGGAGTTG GAGAGCCTCATAGCAAACCTCGGGACTGGGGATGAGACGGTGACAGATCAAGCCTTTGAGGACAGACTGA AGGAAGCAGAGAGGGAGGTTACAGACCTCCTCCGTGAGGCTCAGGAAGTCAAAGATGTAGACCAAAATCT GATGGATCGCCTTCAGAGAGTAAATAACAGTCTGCACAGCCAAATCAGCCGGCTGCACAATATCCGGAAC ACTATAGAAGAGACGGGGATCTTGGCTGAGCGAGCGCGGTCCCGAGTGGAGAGCACAGAGCAGCTGATTG AGATCGCCTCCAGGGAGCTCGAGAAAGCAAAAATGGCTGCCGCCAATGTGTCAATCACTCAGCCAGAGTC TACAGGGGAACCAAACAACATGACCCTCTTGGCAGAAGAAGCCCGAAGGCTTGCAGAGCGTCATAAACAG GAAGCTGATGACATTGTACGAGTGGCAAAGACAGCCAACGAGACCTCAGCTGAGGCATATAATCTGCTTT TGAGGACCCTGGCAGGAGAAAATCAAACTGCATTGGAGATTGAAGAGCTTAACAGGAAGTATGAACAAGC AAAGAACATCTCTCAGGACCTGGAGAAGCAGGCTGCCAGAGTCCATGAGGAAGCCAAGCGAGCAGGTGAC AAAGCCGTGGAGATCTATGCCAGTGTGGCCCAGCTGACCCCTGTGGACTCGGAGGCTCTAGAGAGTGAAG CAAATAAAATCAAGAAAGAAGCTGCAGATCTGGACCGTCTGATTGACCAGAAGCTAAAAGATTACGAGGA CCTTAGGGAAGACATGCGAGGAAAGGAACATGAAGTAAAGAACCTTCTAGAGAAGGGGAAAGCCGAACAG CAGACCGCTGACCAACTCCTAGCTCGAGCCGACGCTGCCAAGGCTCTTGCTGAAGAAGCTGCTAAAAAGG GACGCAATACCTTACAAGAAGCTAACGACATTCTCAACAACCTGAAAGATTTTGATAGACGAGTGAACGA TAACAAGACAGCTGCTGAAGAAGCTCTAAGGAGAATTCCCGCCATCAACCGGACCATAGCTGAAGCCAAT GAGAAGACAAGGGAGGCACAGCTGGCGCTGGGCAATGCAGCAGCTGACGCCACGGAGGCCAAGAACAAAG CCCACGAGGCGGAGAGGATTGCCAGTGGCGTGCAGAAGAATGCCACCAGTACCAAGGCGGATGCGGAGAG AACCTTCTCGGAAGTGACAGACCTGGATAACGAGGTGAATGGCATGTTGAGGCAGCTAGAGGAAGCGGAG AATGAGCTGAAGAGGAAGCAAGATGATGCCGACCAGGACATGATGATGGCGGGAATGGCTTCACAGGCTG CTCAGGAAGCTGAACTTAATGCCAGAAAAGCCAAAAACTCTGTCAGCAGCCTCCTCAGCCAACTGACCGA CCTCTTGGAGCAGCTGGGACAGCTGGACACCGTGGACCTGAACAAGCTCAACGAGATCGAAGGCTCTCTG AACAAAGCCAAAGACGAAATGAAGGTGAGCGACCTGGACAGGAAGGTGTCCAACCTGGAGAACGAGGCCC GGAAGCAGGAGGCAGCCATCTTGGACTATGACCGAGACATAGCAGAGATCATAAAGGACATTCACAACCT GGAGGACATCAAGAAGACACTACCAACAGGCTGCTTTAACACCCCGTCCATCGAGAAACCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_053966
Insert Size	4824 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_053966.2, NP_446418.1
RefSeq Size	7636 bp
RefSeq ORF	4824 bp
Locus ID	117036
Gene Summary	an extracellular matrix protein [RGD, Feb 2006]

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