Top2b (NM_001100858) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217667 representing NM_001100858 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAAGTCCAGCCTCGCGGGCGTTGACGGGGCGCTGACCTGGGTGAACAATGCTGCAAAAAAGGAAG AGTTGGAGACAGCGAACAAAAATGATTCTTCCAAGAAGTTGTCTGTTGAGAGAGTGTACCAGAAGAAGAC CCAACTGGAGCATATTCTCCTTCGTCCTGATACATACATTGGATCAGTGGAACCACTGACACAGTTAATG TGGGTGTATGACGAAGATGTAGGCATGAACTGCAGAGAGGTTACCTTTGTTCCGGGCTTATACAAGATCT TTGATGAAATTTTGGTCAATGCTGCTGACAACAAGCAGAGGGATAAGAACATGACCTGTATTAAGGTTTC TATTGATCCTGAATCTAATATTATAAGCATTTGGAATAATGGAAAAGGCATTCCAGTAGTAGAACACAAA GTAGAGAAAGTTTATGTTCCTGCTTTAATTTTTGGACAGCTTTTAACATCGAGTAATTACGATGATGATG AGAAAAAAGTTACAGGAGGCCGTAATGGTTATGGTGCAAAACTTTGTAATATTTTTAGTACAAAGTTTAC AGTAGAAACGGCTTGCAAAGAATACAAACATAGTTTTAAGCAGACATGGATGAATAATATGATGAAGACT TCTGAAGCCAAAATCAAACATTTTGATGGGGAAGATTATACATGCATAACATTCCAGCCAGATCTAGCCA AGTTTAAGATGGAAAAACTTGACAAGGACATTGTGGCCCTCATGACCAGAAGGGCATACGATTTAGCTGG TTCCTGCAAAGGCGTCAAGGTCATGTTCAATGGCAAGAAGTTGCCTGTAAATGGATTTCGCAGCTATGTA GACCTTTATGTGAAAGACAAATTGGATGAAACTGGGGTCGCTCTCAAAGTTATTCATGAACTTGCAAATG AAAGATGGGATGTTTGTCTAACCTTGAGTGAAAAAGGTTTCCAGCAGATCAGCTTTGTGAATAGTATTGC AACTACAAAAGGTGGACGGCACGTGGATTACGTAGTAGATCAAGTTGTTGGTAAACTGATTGAAGTAGTT AAGAAAAAGAACAAAGCTGGTGTATCAGTGAAGCCATTTCAAGTTAAAAACCACATATGGGTTTTTATTA ATTGTCTTATTGAAAATCCAACCTTTGATTCTCAAACTAAGGAAAACATGACTCTGCAGCCCAAGAGCTT TGGGTCTAAATGCCAGCTGTCAGAAAAGTTCTTTAAAGCGGCCTCTAATTGTGGCATTGTAGAAAGCATA TTGAACTGGGTGAAATTTAAGGCTCAGACTCAGCTGAATAAGAAGTGCTCCTCAGTAAAGTACAGCAAAA TCAAAGGCATTCCCAAACTGGATGATGCCAATGATGCTGGTGGCAAACATTCCCTGGAGTGTACATTAAT ATTAACAGAGGGGGACTCTGCCAAATCTCTAGCTGTCTCTGGATTAGGTGTGATTGGGAGAGACAGATAT GGAGTCTTCCCTCTCAGGGGTAAAATTCTCAATGCACGAGAAGCGTCTCATAAACAGATTATGGAAAATG CAGAAATAAACAATATTATTAAAATAGTTGGTTTGCAATACAAGAAAAGTTATGATGATGCAGAATCTCT GAAAACTTTACGTTATGGGAAGATTATGATTATGACTGATCAGGATCAAGATGGTTCTCACATTAAAGGC CTGCTAATCAATTTTATTCATCACAATTGGCCATCACTTTTGAAGCATGGCTTTCTTGAAGAATTCATTA CTCCCATTGTAAAGGCGAGCAAAAATAAGCAGGAACTTTCCTTTTATAGTATTCCTGAATTTGATGAGTG GGAAAAACATATAGAAAACCAGAAAGCCTGGAAAATAAAGTACTACAAAGGATTGGGTACCAGTACAGCT AAGGAAGCCAAGGAATATTTTGCTGATATGGAAAGGCATCGCATTTTGTTTAGATATGCTGGCCCTGAGG ATGATGCTGCCATTACCTTGGCATTTAGTAAGAAGAAGATTGATGACAGAAAAGAATGGTTAACCAACTT TATGGAGGATCGGAGACAGCGGAGGTTACATGGCCTACCAGAGCAATTTTTATATGGTACAGCAACTAAG CATTTGACATACAATGATTTCATCAACAAGGAATTGATTCTCTTCTCAAACTCGGACAATGAGAGATCTA TACCCTCTCTTGTGGATGGCTTCAAGCCAGGTCAACGAAAAGTTTTGTTTACCTGTTTCAAGAGGAATGA TAAACGTGAAGTTAAAGTCGCACAGCTGGCTGGATCTGTTGCTGAAATGTCTGCTTATCATCATGGAGAA CAAGCGTTGATGATGACTATTGTGAATTTGGCTCAGAATTTTGTGGGCAGCAACAACATTAACTTGCTTC AGCCCATTGGTCAGTTTGGAACTCGGCTTCACGGTGGCAAAGATGCTGCAAGCCCCCGTTATATCTTCAC AATGTTAAGCTCTCTGGCAAGACTACTTTTTCCAGCTGTGGATGATAACTTGCTTAAGTTCCTTTATGAT GATAATCAGCGTGTAGAGCCGGAGTGGTATATCCCCATAATCCCCATGGTTTTGATTAATGGTGCTGAGG GTATTGGGACTGGATGGGCTTGTAAATTGCCCAACTATGATGCCAGAGAAATTGTGAACAATGTCAGGCG GATGCTAGAAGGTTTGGATCCTCACCCCATGCTTCCAAACTATAAGAACTTCAAAGGCACCATCCAGGAG CTTGGTCAGAACCAGTATGCTGTTAGTGGTGAGATATTTGTGGTGGACAGAAACACAGTAGAGATTACAG AACTTCCAGTTAGAACCTGGACACAGGTATACAAGGAGCAGGTCCTAGAACCTATGCTTAATGGAACAGA TAAAACACCAGCACTGATTTCTGATTACAAAGAATATCACACTGATACAACTGTGAAATTTGTGGTGAAG ATGACAGAAGAGAAGCTCGCCCAAGCGGAAGCTGCCGGCCTGCATAAAGTTTTTAAACTCCAAACCACTC TTACTTGCAATTCCATGGTACTCTTTGATCATATGGGATGTCTGAAGAAGTATGAAACTGTGCAAGACAT TTTGAAAGAATTCTTTGATTTACGGTTAAGTTATTATGGTTTACGGAAGGAGTGGCTTGTAGGAATGTTG GGAGCAGAATCCACAAAGCTTAACAATCAAGCCCGTTTCATTTTAGAGAAGATACAAGGGAAAATCACGA TAGAGAATAGGTCAAAGAAAGATTTGATTCAAATGTTAGTCCAGAGAGGTTATGAATCTGACCCAGTGAA AGCCTGGAAAGAAGCACAAGAAAAGGCTGCGGAAGAGGAAGATGCACAAAACCCACATGATGACAGTTCC TCTGACTCAGGAACTCCTTCAGGCCCTGATTTTAATTATATTTTAAATATGTCTCTATGGTCTCTTACTA AAGAAAAAGTTGAAGAACTCATTAAACAGAGAGATACAAAAGGACGAGAGGTTAATGATCTCAAAAGGAA ATCTCCCTCAGATCTTTGGAAAGAAGATTTAGCAGCATTTGTTGAAGAATTGGATAAAGTGGAAGCACAA GAACGAGAAGACATTCTGGCAGGGATGTCTGGGAAAGCAGTGAAAGGCAAAGTCGGCAAAGCTAAAGTAA AGAAGCTCCAGCTGGAGGAGACGATGCCCTCGCCTTACGGGAGAAGAATCGTTCCTGAGATTACTGCCAT GAAGGCAGACGCCAGCAGAAAACTGCTGAAGAAGAAGAAGGGTGATCCTGATACTACAGTTGTGAAAGTA GAATTTGATGAAGAATTTAGTGGAACACCAGCAGAAGGTACAGGAGAAGAGACATTGACACCATCTGCTC CTGTAAATAAAGGCCCCAAACCCAAAAGGGAGAAAAAGGAGCCTGGTACCAGGGTTAGAAAAACACCTGC ATCAACTGGCAAACCTAATACAAAGAAAGTGAAGAAGCGGAATCCTTGGTCAGATGATGAGTCCAAATCA GAGAGTGACTTGGAAGAAGCAGAGCCTGTGGTTATCCCAAGAGATTCTTTGCTTAGGAGAGCAGCTGCTG AAAGACCTAAGTACACTTTTGATTTCTCAGAAGAGGAGGAGGATGATGCTGATGATGATGACGACAATAA TGATTTAGAAGAATTGAAAGTTAAAGCATCTCCCATAACAAATGATGGGGAGGATGAATTTGTTCCTTCA GATGGCATAGATAAAGATGAGTATGCCTTTTCTCCAGGCAAATCCAAAGCTACACCAGAAAAATCTTCAC ATGACAAAAAAAGTCAGGATTTTGGAAATCTTTTTTCATTTCCTTCATATTCTCAGAAGTCAGAAGATGA TTCAGCTAAATTTGACAGTAATGAAGAAGACACCACATCTGTTTTTGCACCATCATTTGGTCTGAAGCAA ACAGATAAAGTTCCAAGTCAAACAGTCGCTGCTAAAAAGGGTAAAGCACCTTCAGATGCAGCAGCCCCTA AGGCCAAGAGAGCCCCTAGACAGAGGAAGGTAGTAGAGCCTGCAAACTCCGACTCGGATTCTGAACTCGG CAACATTCCAAAGAAGACGGCCGCACCGAAAGGTAAAGGCCGAGGGGCAAAGAAAAGGAAAGCATCAGGC TCAGAAAATGAAGGTGATTATAACCCTGGCAGGAAACCATCTAAAACAGCCAGCAAGAAACCGAAGAAAA CGTCTTTTGATCAGGATTCAGATGTAGACATCTTCCCTTCAGACTTCACTTCTGAACCACCTGCTCTCCC ACGGACTGGTCGGGCTAGGAAAGAAGTAAAATATTTTGCAGAGTCTGATGAAGAAGAAGACGTTGATTTT GCAATGTTTAATTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001100858
Insert Size	4845 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001100858.1, NP_001094328.1
RefSeq Size	4845 bp
RefSeq ORF	4845 bp
Locus ID	361100
Gene Summary	a nuclear enzyme that catalyzes the breaking and rejoining of two strands of duplex DNA [RGD, Feb 2006]

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