Kdm5a (NM_001277177) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	Jarid1a
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217682 representing NM_001277177 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGGCGTGGGCCCGGGGGGCTACGCGGCGGAGTTCGTGCCGCCGCCCGAGTGCCCGGTCTTTGAGC CCAGTTGGGAGGAGTTCACAGACCCGCTAAGTTTTATCGGTCGCATCCGGCCTCTGGCTGAGAAAACTGG CATCTGCAAAATCCGTCCACCCAAGGATTGGCAACCTCCATTTGCTTGTGAAGTAAAAACCTTCCGATTC ACTCCCAGAGTCCAGCGCCTGAATGAACTTGAGGCAATGACTAGAGTAAGATTGGACTTCTTGGATCAAC TGGCAAAATTTTGGGAACTTCAAGGATCTACATTGAAGATTCCCGTGGTGGAGAGAAAAATCCTGGATTT GTATGCTTTAAGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATAGTCACCAAAGAGAAGAAATGGTCT AAAGTGGGTAGCCGTTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCACACTATGAAA GAATTCTCTACCCATATGAGCTTTTCCAATCTGGTGTCAGCCTTATGGGTGTACAAATGCCTGATTTAGA TCTTAAGGAAAAAGTTGAAGCTGAGGTTCTCAGTACTGACATCCAGCCTTCCCCAGAGCGGGGAACAAGA ATGAATATCCCACCAAAGAGAACAAGACGAGTTAAATCCCAGTCAGACTCTGGAGAAGTAAATAGAAATA CAGAACTGAAGAAACTTCAGATTTTTGGGGCTGGACCCAAAGTAGTAGGCCTGGCAGTAGGAGCAAAAGA TAAAGAAGATGAGGTCACCCGAAGACGGAAAGTTACCAACAGGTCAGATGCATTTAACATGCAGATGAGA CAACGGAAAGGAACTCTCTCTGTTAACTTTGTCGATCTCTATGTTTGCATGTTTTGTGGTCGAGGAAACA ATGAGGATAAACTGCTTTTGTGTGACGGGTGTGATGACAGCTACCATACATTTTGTCTGCTTCCACCACT TCCTGATGTGCCCAAAGGAGACTGGAGGTGTCCTAAATGTGTTGCTGAGGAATGTAACAAACCTCGAGAA GCCTTTGGATTTGAGCAAGCTATAAGAGAATATACACTTCAAAGCTTCGGAGAGATGGCAGATAATTTTA AGTCTGATTATTTCAATATGCCAGTGCATATGGTTCCTACAGAGCTAGTAGAAAAGGAGTTCTGGCGGCT AGTAAGTAGTATTGAAGAAGACGTCATTGTAGAGTATGGAGCTGATATCTCTTCAAAAGATTTTGGAAGT GGATTTCCTATAAAGGATGGTCAGAGAAAGATGTTACCAGAAGAGGAGGAGTATGCACTTTCTGGTTGGA ATTTGAATAACATGCCTGTTCTGGAGCAGTCTGTTCTTGCTCATATTAATGTGGACATTTCTGGTATGAA GGTGCCGTGGCTCTATGTGGGAATGTGCTTTTCTTCTTTTTGCTGGCATATTGAAGATCACTGGAGTTAT TCAATTAACTACTTGCATTGGGGCGAGCCAAAGACATGGTATGGTGTACCATCTCATGCTGCAGAACAAC TGGAGGAGGTGATGAGGGAGCTGGCCCCTGAGTTATTTGAATCACAGCCTGACCTCCTACATCAGTTAGT AACCATCATGAACCCAAATGTGCTCATGGAGCATGGTGTGCCTGTGTACAGAACTAATCAGTGTGCTGGA GAGTTTGTGGTGACGTTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGATACAACTTTGCTGAAGCCG TGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAGTGTGTAAATCACTATCGACGGCTGAGGCG CCATTGTGTCTTTTCACATGAGGAACTCATTTTCAAGATGGCAGCAGATCCAGAATGCTTAGATGTGGGG CTGGCTGCCATGGTATGCAAAGAGTTGACTCTCATGACTGAAGAAGAAACACGATTGAGGGAATCGGTTG TTCAAATGGGTGTCGTGATGTCAGAAGAAGAAGTCTTCGAACTTGTTCCTGATGATGAGCGGCAGTGTTC AGCATGCAGAACCACATGTTTTCTCTCAGCTCTCACATGCTCCTGTAATCCAGAGCGACTTGTATGTTTG TATCATCCAACTGATTTGTGCTCCTGCCCCATGCAGAACAAATGTCTTAGGTACCGATACCCACTTGAAG ACCTCCCATCTCTGCTTTATGGTGTCAAAGTGAGAGCACAGTCCTATGACACTTGGGTCAATCGTGTCAC AGAAGCATTATCAGCTAGCTTCAACCATAAGAAAGATTTGATTGAATTGAGAGTAATGCTGGAAGATGCT GAGGATAGGAAATACCCAGAGAATGATCTCTTTCGAAAGCTCAGGGATGCGGTAAAAGAAGCTGAGACCT GTGGTTCTGTGGCTCAGCTGCTTCTGAGCAAAAAACAAAAACACAGGCAGAGCTCAGATAGTGGGAAGAC TCGGACCAAACTGACAGTAGAAGAATTGAAGGCCTTTGTCCAACAACTTGTTAGTCTTCCGTGTGTCATC AGCCAAACTCGACAAGTAAAGAATCTGCTGGATGATGTAGAAGAATTTCATGAACGTGCCCAGGAGGCCA TGATGGATGAAACTCCAGATTCTTCCAAACTCCAAGTGTTAATAGACATGGGCTCTAGTCTCTATGTGGA ACTACCTGAATTGCCCCGACTAAAGCAAGAGCTACAGCAGGCTCGGTGGCTGGATGAAGTAAGACTGACT CTATCAGACCCACAACAAGTCACTTTGGATGTCATGAAGAAATTGATTGACTCTGGGGTAGGCTTGGCAC CTCACCATGCTGTGGAAAAGGCAATGGCTGAACTACAGGAACTTCTTACAGTCTCTGAACGATGGGAAGA GAAGGCGAAGGTCTGCCTGCAGGCAAGACCACGGCACAGTATGGCAAATTTAGAAAATATTGTAAATGAA GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTAAAAGAAGCCTTACAAAAGGCTCGTGAAT GGACAGCTAAAGTGGAAGCTATTCAGAGCGGCAGCAACTATGCTTACTTGGAACAGCTTGAGAGCTTGTC TGCTAAAGGACGCCCTATCCCTGTGCGCCTCGATGCCTTGCCTCAAGTGGAGTCACAAGTAGCAGCAGCG CGAGCCTGGAGGGAGCGGACGGGGCGGACCTTCCTTAAGAAGAATTCCAGCCATACATTGTTACAGGTGC TAAGTCCACGAACAGACATTGGTGTATATGGCAGTGGCAAAAACAGAAGGAAAAAAGTAAAAGAAATAAT AGAAAAAGAAAAGGAGAAGGACCTAGACCTTGAACCTTTGAGTGACTTAGAAGAAGGACTGGAGGAGACG AGGGACGCAGCCATGGTGGTGGCAGTTTTCAAAGAACGAGAACAAAAGGAGATTGAAGCCATGCATTCCC TCAGAGCAGCCAACCTAGCCAAGATGACCATTGTGGACCGCATAGAAGAAGTAAAGTTTTGTATTTGCCG CAAGACAGCGAGTGGGTTTATGCTACAGTGTGAGCTCTGCAAAGACTGGTTCCATAATGGCTGTGTTCCC CTTCCTAAGTCAGGTTCCCAGAAAAAGGGATCCAGCTGGCAAGCCAAAGACGTAAAATTCCTTTGCCCTC TCTGCATGAGATCCCGGAGGCCGAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCC TGTACGATTGCCTGAAGGAGAAGCACTGCAGTGTTTGACAGAGCGTGCTATGAGTTGGCAAGATAGAGCA CGACAAGCTTTAGCCACAGATGAACTGTCTTCTGCCCTGGCCAAACTCTCTGTGTTAAGCCAGCGTATGG TAGAACAAGCAGCCCGAGAAAAAACTGAAAAGATTATCAGTGCAGAACTCCAGAAAGCAGCCGCCAATCC AGACTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCTTTTAACCGGGTGGTGAGCAGTGTATCCTCT TCCCCCCACCAAGCGATGGACTATGATGATGAAGAAACAGACTCTGATGAAGATATTCGAGAGACATATG GCTATGACATGAAGGATACAGCCAGTGTAAAGTCCTCTAGCAGTCTGGAACCCAATCTCTTTTGCGATGA AGAGATTCCTATCAAATCGGAGGAAGTGGTGACCCACATGTGGACAGCACCTTCATTCTGTGCAGAGCAT GCATATTCTTCTGCTTCTAAGAGTTGTTCTCAAGGTTCTAGCACTCCAAGAAAGCAGCCTCGGAAGAGCC CTTTGGTTCCCAGAAGTTTGGAGCCTCCAGTATTAGAATTGTCTCCTGGAGCAAAAGCCCAGTTGGAAGA ACTCATGATGGTTGGAGACCTCCTAGAAGTGTCTCTGGATGAGACACAACACATATGGCGGATTTTGCAG GCCACACATCCACCCTCAGAGGACAGATTCTTACATATCATGGAGGATGATAGCGTAGAAGAGAAACCAT TAAAAATGAAAGGAAAGGATTCTTCAGAGAAGAAACGGAAGCGGAAATTAGAAAAGGTAGAACAGTTTTT TGGAGAAGGAAAACAGAAGACCAAGGAGCTAAAGAAAATAGATAAACCTAAAAAGAAGAAATTAAAATTA AATGTGGACAAATCAAAAGAGCTGAATAAATTGGCTAAGAAGTTAGCAAAAGAAGAAGAGCGAAAGAAAA AGAAAGAAAAGGCTGCTGCAGCCAAAGTGGAGCTTGCGAAAGAGAGCACCGAGAAGAAAAGAGAGAGAAA GGTGCTGGATATCCCTTCAAAGTATGACTGGTCGGGCGCAGAGGAGTCTGATGATGAGAATGCTGTATGT GCAGCACAGAACTGCCAAAGGCCGTGTAAGGACAAGGTAGACTGGGTACAATGTGATGGTGGCTGTGATG AGTGGTTTCATCAAGTTTGTGTTGGTGTATCCCCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAA CTGTGCAAAGAAGCAGGGGCCAGATAGCCCAGGCCACGCACCACCTCCTCCCTTCATAATGAGCTACAAA CTACCAATGGAGGATCTTAAAGAGACCAGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277177
Insert Size	5073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277177.1, NP_001264106.1
RefSeq Size	10937 bp
RefSeq ORF	5073 bp
Locus ID	312678

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