Arfgef1 (NM_001277056) Rat Untagged Clone
CAT#: RN217698
Arfgef1 (untagged) - Rat ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1)
CNY 17,670.00
货期*
8周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
经常一起买 (3)
Specifications
Product Data | |
Type | Rat Untagged Clone |
Tag | Tag Free |
Synonyms | BIG1; RGD1560793 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RN217698 representing NM_001277056
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTATGAGGGCAAGAAGACGAAGAACATGTTCCTGACCCGGGCCCTGGAGAAGATTTTGGCCGACAAGG AAGTGAAGAAGGCGCATCACTCCCAGCTGCGCAAAGCGTGCGAGGTGGCCCTAGAGGAAATAAAAGTTGA AACTGAGAAACAAAGTCCTCCTCATGGAGAAGCAAAAGCTGGATCAGGCACCCTTCCACCAGTGAAATCA AAGACAAATTTTATTGAAGCAGACAAGTACTTCTTACCCTTTGAGTTGGCATGCCAGTCCAAATGTCCCC GCATAGTTAGTACATCTCTAGACTGTTTGCAGAAACTTATTGCTTATGGGCACTTGACTGGCAGTGCTCC AGATAGTACGACACCAGGCAAAAAGTTAATTGATAGAATTATTGAAACAATATGTGGCTGCTTCCAAGGC CCTCAAACAGATGAAGGAGTTCAGTTGCAGATAATAAAGGCTTTACTTACTGCAGTAACATCACAACACA TAGAAATTCACGAAGGAACTGTGCTGCAAGCTGTGAGGACATGCTACAATATCTATTTAGCCAGCAAAAA TCTCATCAATCAGACAACAGCCAAAGCTACTCTTACTCAGATGCTAAATGTTATCTTTGCACGCATGGAA AACCAAGCATTACAGGAAGCCAAACAAATGGAAAGAGAAAGACATCGGCAGCAGCATCACTTGTTACAGT CTCCAGTAAGCCATCACGAGCCCGAGTCACCTCATCTTAGATATTTGCCACCTCAGACTGTTGATCATAT AGCCCAAGAACAGGAAGGGGATCTTGATCCCCAAACACACGATGTGGATAAAAGTCTCCAAGATGACATA GAGCCTGAGAACGGATCTGATATTTCCAGTGCAGAGAATGAACAGACAGAAGCTGATCAGGCAACTGCAG CTGAAACATTATCTAAAGATGATGTGTTGTGTGATGGAGAGTGTGAGGAAAAGCCACAGGATATTGTGCA GAGCATTGTGGAAGAGATGGTGGACATCATTGTTGGAGACATGGGGGAAGGAACAGCTGTAAGTGCAAGT GCAGATGGCAACGCTGGAGCTGTAGAGGATGGCAGTGACAGTGAGAACGTGCAAGCCAATGGGATCCCGG GAACACCCATCTCTGCCGCATACACACCATCGTTACCTGACGACAGATTGTCTGTCTCCTCCAATGATAC TCAGGAATCTGGAAATTCTTCAGGACCTTCACCTGGTGCTAAGTTTTCCCACATTTTACAAAAGGATGCC TTTCTAGTGTTCAGGTCATTGTGTAAGCTGTCAATGAAACCACTGTCAGATGGACCACCAGATCCAAAGT CTCATGAGTTACGGTCCAAGATTCTTTCATTGCAGTTGCTTCTGTCCATTCTGCAGAATGCAGGACCTGT TTTCAGGACAAATGAGATGTTTATTAATGCTATCAAGCAGTATCTGTGTGTTGCACTCTCAAAAAACGGA GTCTCATCTGTTCCAGAGGTTTTTGAGCTTTCTCTTTCCATATTTCTAACTTTGTTGTCAAACTTCAAGA CACATCTGAAGATGCAAATTGAGGTATTCTTTAAAGAAATTTTCCTGTACATTTTGGAAACTTCTACAAG CTCATTTGATCATAAGTGGATGGTGATTCAGACACTGACAAGGATCTGTGCAGATGCTCAGAGTGTGGTG GATATTTATGTAAATTATGACTGTGACTTGAATGCAGCAAATATATTTGAAAGACTAGTAAATGACCTGT CAAAAATTGCCCAAGGAAGGGGCAGTCAAGAACTTGGGATGAGTAATGTTCAGGAATTGAGCCTGCGGAA AAAGGGGTTAGAATGTTTAGTTTCAATTCTTAAGTGTATGGTTGAATGGAGTAAGGATCAGTACGTGAAT CCCAACTCTCAAACCACCCTTGGTCAGGAAAAACCCTCAGAGCAAGAGATAAGTGAAATCAAACACCCCG AGACTATAAACAGATATGGAAGTTTAAACTCCTTAGAATCCACATCATCTTCAGGGATTGGCAGCTACAG CACACAGATGTCTGGCACTGATAATCCAGAACAATTCGAGGTCCTAAAGCAGCAAAAAGAAATAATAGAA CAAGGGATTGATTTATTTAATAAGAAACCAAAGAGAGGAATACAGTACCTCCAAGAACAAGGGATGCTTG GCACCACTCCTGAAGATATAGCTCAGTTCTTACATCAAGAAGAAAGATTAGACTCTACACAGGCGGGGGA GTTCCTGGGAGATAACGATAAGTTCAATAAGGAAGTCATGTATGCATATGTAGACCAGCATGACTTTTCG GGAAAAGACTTTGTTTCAGCTCTTCGTCTGTTTCTGGAAGGATTCCGGCTTCCAGGAGAAGCTCAGAAAA TTGATCGATTAATGGAAAAATTTGCTGCAAGATACCTAGAGTGCAACCAAGGACAAACCCTTTTTGCTAG TGCAGATACTGCTTATGTTTTGGCCTACTCAATTATCATGTTGACCACAGACCTTCACAGTCCACAGGTT AAAAACAAAATGACGAAAGAACAGTATATTAAAATGAATCGTGGTATCAATGACAGTAAAGATCTCCCTG AAGAGTATCTATCTGCCATTTATAATGAGATAGCTGGGAAAAAAATATCAATGAAAGAAACAAAAGAACT AACAATCCCTACAAAATCAACTAAACAGAATGTAGCCAGTGAAAAGCAAAGACGACTTCTGTATAATTTA GAAATGGAACAGATGGCCAAGACAGCTAAAGCACTCATGGAAGCTGTGAGCCATGTTCAGGCACCTTTTA CAAGTGCAACACACTTGGAGCACGTGAGGCCTATGTTTAAGTTGGCTTGGACCCCTTTTCTGGCTGCCTT CAGTGTGGGTCTACAAGACTGTGATGATACTGACGTGGCCTCTCTCTGCCTCGAAGGGATACGATGTGCG ATCAGAATTGCATGCATCTTCAGCATTCAGCTGGAAAGAGATGCATATGTCCAGGCACTAGCCAGATTTA CCTTACTTACAGTGAGTTCTGGTATTACTGAAATGAAACAGAAGAATATCGACACAATAAAAACACTCAT CACAGTGGCTCACACAGATGGAAATTATTTAGGAAATTCGTGGCATGAGATCCTGAAGTGCATTAGTCAG TTAGAGCTTGCACAACTTATAGGAACTGGAGTGAAGCCTCGGTACATTTCTGGGACCGTGAGAGGCAGAG AAGGGTCTCTCACTGGGACAAAAGATCAGGCTCCGGATGAATTTGTTGGTCTGGGGCTAGTTGGAGGAAA TGTGGACTGGAAGCAGATAGCCAGTATTCAGGAGTCTATTGGAGAAACCAGTTCTCAAAGTGTCGTGGTT GCTGTAGACAGAATATTCACAGGATCCACAAGGCTAGATGGAAATGCTATTGTCGATTTTGTTCGTTGGC TCTGTGCCGTGTCTATGGATGAATTGCTTTCCACTACACACCCAAGAATGTTTAGTCTACAAAAAATAGT AGAAATATCATACTATAACATGGGAAGAATAAGACTGCAGTGGTCTCGGATTTGGGAAGTTATTGGAGAT CATTTTAATAAGGTTGGTTGCAATCCTAATGAAGATGTAGCCATATTTGCAGTAGACTCCCTGAGGCAGC TATCGATGAAGTTTTTAGAGAAAGGAGAGCTTGCAAACTTCAGATTCCAAAAGGATTTCTTAAGACCTTT TGAACATATAATGAAACGAAACAGGTCTCCAACAATTCGAGATATGGTCGTGCGTTGTATAGCGCAGATG GTTAACTCTCAGGCTGCCAATATCCGTTCAGGATGGAAGAACATTTTCTCGGTGTTCCACCTAGCTGCAT CAGACCAGGATGAGAGCATAGTAGAGCTAGCATTCCAGACGAGCGGGCACATCGTCACTCTTGTATTTGA AAAGCACTTTCCAGCCACTATTGATTCTTTCCAGGATGCAGTGAAGTGTTTATCTGAATTTGCGTGTAAT GCGGCTTTCCCAGACACAAGTATGGAAGCAATCCGACTTATTCGCCATTGTGCAAAATATGTGTCTGATA GACCTCAGGCTTTCAAAGAGTACACAAGTGATGACATGAACGTGGCACCTGAAGACAGGGTGTGGGTGAG AGGGTGGTTCCCAATCCTCTTTGAGTTGTCCTGTGTCATCAATAGATGCAAATTAGATGTAAGAACCAGG GGCTTAACAGTGATGTTTGAAATAATGAAAACATACGGCCACACTTACGAAAAACACTGGTGGCAGGACT TGTTCAGAATTGTTTTCAGAATCTTTGACAACATGAAGTTGCCAGAACAGCAGACAGAGAAAGCTGAATG GATGACAACAACTTGTAACCATGCCCTTTATGCGATATGCGACGTGTTCACCCAGTATTTAGAAGTCCTC AGTGATGTACTTTTGGATGATATTTTTGCCCAGCTTTACTGGTGTGTACAACAAGACAATGAGCAGTTAG CACGATCTGGTACGAACTGCTTAGAAAATGTGGTTATTCTGAATGGTGAGAAGTTTACTCTAGAAATCTG GGATAAAACCTGTAATTGCACCCTGGATATCTTCAAAACCACAATTCCTCATGCGCTTCTGACTTGGCGT CCTACTTCTGGAGAGGCTGCCCCCCCATCTCCATCAGCTATGAGTGAAAAGCAGTTGGATGCAATATCAC AGAAATCTGTAGACATACATGATTCTGCTCAACCAAGATCTTCAGATAACAGGCAGCAAGCACCACTTGT TTCTGTTTCTCCTGCGAGTGAAGAAGTTAGCAAAGGGAGACCCACAGCAAAATTCCCAGAACAAAAATTG TTTGCTGCCCTGTTGATTAAATGTGTTGTGCAGCTGGAACTCATCCAGACCATCGACAACATTGTGTTCT TCCCAGCCACAAGCAAGAAGGAAGATGCAGAAAACTTAGCTGCAGCCCAGAGAGATGCAGTGGACTTCGA TGTTCGAGTTGATACTCAAGACCAAGGAATGTACCGCTTTTTAACATCACAACAACTCTTCAAGCTACTG GACTGCTTATTAGAGTCACATAGATTTGCAAAAGCATTTAATTCCAACAATGAACAGAGGACTGCTCTAT GGAAAGCAGGCTTCAAAGGCAAGTCCAAGCCCAATCTCCTAAAACAAGAGACAAGCAGCCTGGCGTGTGG ACTGCGCATCCTCTTCCGGATGTACACAGACGAGAGCAGAGTCAGCGCCTGGGAGGAGGTCCAGCAGAGG CTTTTAAACGTCTGCAGTGAGGCACTGAGTTACTTCCTCACTCTAACATCTGAAAGTCACCGGGAAGCCT GGACCAACTTACTGTTACTGTTCCTCACTAAAGTCCTCAAGATCAGTGACAACAGGTTTAAAGCTCACGC ATCATTCTACTACCCTCTGTTATGTGAAATTATGCAATTTGACTTGATTCCTGAACTTCGTGCTGTTCTT AGAAGATTTTTCCTGCGAATTGGAATAGTATTTCAGATATCACAACCACCTGAACAGGAACTTGGGATAA ACAAGCAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001277056 |
Insert Size | 5541 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001277056.1, NP_001263985.1 |
RefSeq Size | 6945 bp |
RefSeq ORF | 5541 bp |
Locus ID | 312915 |
UniProt ID | D4A631 |
Gene Summary | Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RR217698 | Arfgef1 (myc-DDK-tagged) - Rat ADP-ribosylation factor guanine nucleotide-exchange factor 1(brefeldin A-inhibited) (Arfgef1) |
CNY 17,670.00 |
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