Myh11 (NM_001170600) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217716 representing NM_001170600 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGAAAGGGCAGCTCAGTGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATAAACAGCC CAATGGCCCAGGCCGACTGGGTAGCCAAGAAGCTAGTATGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTTGTAGAGCTGGTGGAAAACGGAAAGAAGGTCACA GTGGGCAAAGATGACATCCAAAAAATGAACCCGCCCAAGTTCTCCAAGGTGGAAGACATGGCCGAGCTGA CATGCCTCAATGAGGCTTCTGTGCTGCACAACCTGAGGGAGCGGTACTTCTCAGGCCTCATCTACACCTA CTCGGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGCACCTACCCATCTACTCAGAAAAGATTGTGGAC ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCTGATACGGCCTACAGAA GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCTGGAAAGACAGAGAA CACAAAGAAAGTCATACAGTATTTGGCTGTGGTGGCATCCTCCCACAAAGGCAAGAAAGACAGCAGCATC ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCAAAAACTG TCAAGAATGACAACTCCTCCCGCTTTGGCAAGTTCATTCGCATCAACTTTGATGTCACTGGTTACATTGT GGGTGCCAATATCGAAACATATCTTCTGGAAAAGTCTCGGGCTATTCGACAGGCTAGGGACGAGAGAACG TTCCACATCTTCTACTATCTGATCGCTGGAGCCAAGGAGAAGATGAGAAATGACCTACTTTTGGAGAGCT TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA GGAGACACTAGAGGCCATGTCCATCATGGGCTTCAGTGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA TCATCCGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG ATAACACAGCTGCTCAGAAAGTTTGCCACCTTGTGGGGATTAATGTGACAGATTTCACCAGAGCCATCCT GACCCCACGTATAAAAGTTGGACGGGATGTGGTGCAGAAAGCTCAGACCAAAGAACAGGCTGACTTTGCC ATTGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTGAACAAAGCCT TGGATAAGACCCATCGTCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCTGGGTTTGAAATCTTTGA GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAATGAGAAGTTGCAGCAGCTGTTCAATCACACC ATGTTCATCCTGGAGCAGGAAGAGTACCAACGAGAGGGCATCGAGTGGAACTTCATCGACTTTGGCCTGG ACCTACAGCCTTGTATTGAACTGATTGAGCGGCCGAACAACCCTCCTGGTGTGCTGGCCCTGCTGGATGA AGAATGCTGGTTCCCCAAAGCTACGGACAAGTCTTTTGTGGAGAAGCTGTGCTCAGAGCAGGGCAATCAC CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG TGGACTATAATGCAAGTGCGTGGCTGACCAAGAACATGGACCCGCTAAATGATAATGTGACCTCACTCCT CAATGCCTCCTCGGACAAGTTTGTGGCCGACCTGTGGAAGGATGTGGACCGCATTGTGGGGTTGGACCAG ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG GCCAGCTCTACAAAGAGCAGCTGGGGAAGCTGATGACCACACTGCGCAACACCACGCCTAACTTTGTGCG CTGCATCATCCCCAACCATGAAAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTTCTGGAGCAGCTGCGG TGCAATGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT TCCGGCAACGCTACGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGACGGAAAGCAAGCCTG CATTCTCATGATCAAAGCCCTTGAACTTGACCCCAATCTGTACAGGATTGGGCAGAGCAAAATCTTCTTT CGTACGGGAGTCCTGGCCCACCTAGAGGAAGAACGAGACCTGAAGATTACTGATGTCATCATGGCTTTCC AGGCAATGTGTCGTGGCTACCTGGCCAGAAAAGCCTTCACCAAGAGGCAGCAACAGTTAACAGCCATGAA GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGAGGCTCTTCACTAAA GTAAAGCCGCTGCTCCAGGTGACGCGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAGAAGA TCAAGGAGCGGCAGCAGAAGGCAGAGTCTGAGCTGAAGGAGCTGGAGCAAAGACACACTCAGCTGGCTGA GGAGAAGACCCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAAGAGATGCGG GTCCGCTTGGCAGCGAAGAAGCAGGAGCTAGAGGAGATCCTCCATGAGATGGAGGCCCGCTTGGAGGAGG AGGAGGACCGGAGCCAACAACTGCAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTGGACCTGGAGGA GCAACTGGAGGAGGAAGAAGCTGCCAGACAGAAACTACAGCTAGAGAAGGTCACAGCTGAGGCCAAGATC AAGAAACTGGAGGATGACATCTTGGTTATGGATGACCAGAACAATAAGCTTTCCAAAGAAAGAAAACTCC TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA ACTAAAGAGCAAGCATGAGTCCATGATCTCAGAGCTAGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCACGAGCAGATCGCTGACC TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC CAGGCTCGATGAAGAGATCACCCAGAAAAACAATGCCCTAAAGAAGATTCGAGAGCTGGAGGGCCATGTC TCAGACCTACAGGAGGACCTGGACTCAGAGCGAGCTGCTAGGAACAAGGCTGAGAAACAGAAGCGAGACC TGGGGGAGGAACTGGAGGCGCTCAAGACAGAGCTGGAAGATACACTGGACAGCACAGCTACCCAGCAGGA GCTCAGAGCCAAGAGGGAACAGGAAGTGACAATGCTGAAGAAGGCCCTGGATGAAGAGACGCGATCCCAT GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAAC AGTTCAAAAGGGCCAAGGCAAACCTGGATAAAAGCAAGCAGACGCTGGAGAAGGAGAACGCAGACCTGGC TGGGGAGCTGCGTGTCCTGGGCCAGGCAAAGCAGGAGGTAGAGCACAAGAAGAAGAAGCTGGAGGGGCAG CTGCAGGAGCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGACTGAGCTCAGTGACAAGGTCCACA AACTACAGAATGAAGTGGAGAGTGTCACAGGCATGCTCAATGAGGCAGAAGGCAAGGCCATTAAACTGGC CAAGGAAGTGGCTTCCCTTGGATCTCAACTTCAGGACACCCAAGAGCTGCTTCAAGAAGAAACCCGGCAG AAGCTCAATGTGTCCACCAAGCTGCGCCAGTTGGAAGATGAGAGGAACAGCCTGCAGGACCAGCTGGATG AGGAGATGGAAGCTAAGCAAAACCTTGAGCGCCATGTCTCCACCTTGAACATTCAGCTCTCAGACTCTAA GAAGAAGCTGCAGGACCTTGCAAGCACTATTGAGGTCATGGAGGAGGGGAAGAAGAGGTTGCAGAAAGAG ATGGAGGGTCTCGGCCAGCAGTATGAGGAGAAGGCCGCTGCCTATGACAAACTGGAGAAAACTAAGAACA GGCTTCAGCAGGAGCTAGATGACTTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA AAAGAAGCAGAAGAAATTCGATCAGTTGTTAGCTGAGGAAAAGAACATCTCCTCCAAGTATGCGGATGAG AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAAACGAAGGCTTTGTCTCTGGCCCGGGCCCTGGAGG AAGCCCTGGAAGCCAAAGAGGAACTGGAAAGGACCAACAAGATGCTCAAGGCCGAGATGGAAGACCTGGT CAGCTCCAAGGATGACGTGGGCAAGAATGTGCACGAGCTGGAGAAGTCCAAACGAGCCCTGGAGACCCAG ATGGAAGAGATGAGAACTCAGCTAGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAAGACGCCAAGCTGC GGCTGGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGAGATCTCCAGGCTCGGGATGAGCAGAA TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCATGAGTATGAGACAGAACTGGAAGATGAACGGAAG CAAAGGGCTCTGGCGGCAGCAGCCAAGAAGAAGCTGGAAGGGGATCTGAAAGACCTGGAACTCCAGGCTG ACTCAGCCGTCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTGCAGGCTCAGATGAAGGACTT CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGAGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG AAAGCCAAGAGTCTGGAGGCAGAGCTCATGCAGCTCCAGGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCTGAGGAGCTGGCTAGCAGCTTATCAGGAAGGAATACACT GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAAGAGCAGGGC AACATGGAGGCCATGAGTGACAGAGTCCGCAAGGCCACATTGCAGGCTGAGCAACTGAGCAATGAGCTGG TCACTGAGCGCAGTGCAGCTCAGAAGAATGAGAGTGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT CCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTTAAGTCCACTGTTGCGGCACTAGAG GCCAAGATTGTGCAGCTGGAGGAGCAGATTGAACAAGAGGCCAGAGAAAAACAGGCAGCCACCAAGTTGC TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTACTGCAGGTGGAGGATGAGCGCAAGATGGTGGAGCA GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTCAAGAGGCAGCTGGAAGAGGCCGAG GAGGAATCCCAGCGCATCAATGCCAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACTGAGAGCA ATGAGGCCATGGGCCGTGAGGTGAATGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT TCCTTCCAGAAGGGCTGGAGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGATGCT CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001170600
Insert Size	5919 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001170600.1, NP_001164071.1
RefSeq Size	6555 bp
RefSeq ORF	5919 bp
Locus ID	24582
Gene Summary	transcription decreases following transformation with mutant p53 [RGD, Feb 2006]

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