Lrrk1 (NM_001191624) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217724 representing NM_001191624 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCCGTGTCACAGCGACCACCCAGTATGTACTGGTGTGTGGGGACAGAAGGATCAGTTGTGTGTC CAGGGCCTGCCATGGAGACCCATAATGGTGCCGAAGACATGGGCAGCAAACTGTCTTTACCAGGTGGCAA CTCCCCAGTGCAGAGCCCCAACATGGAAGAAATCCACACAGCATACAAGCAGAGAAACCTCTCCCGAGCC CGGGACCTGCTCAGGGAAGTTTGTGAAGAGAGCGAATCCCCACAGGAAAAGGGCCAGTTGCTGAGCATCT CAGCAGCCCACGGGGATCTAGAGACCGTTCGGTTCCTGCTCACTGAGAAGCGCGTGGAGCTGCCGATGGA GCCCACCGATGACAACCCAGCTGTGGTGGCAGCGCATTTTGGACATGCTGAAGTAGTACACGAATTGCTG GAGTCCTTATCAGGTCCCTGTGCCTCGCAGCGGCTGCTGAACTGGATGCTGGCCCTGGCTTGCCAGCGAG GGCACCTGGAGGTTGTGAAGCTGCTGGTCCTGACGCACGGGGCTGACCCGGAGAACTACGCCGTCAGGAA GAATGAGTTCCCGGTCATTGTGAGGTTGCCCCTCTACGCGGCCATCAAGGCAGGGAATGAAGACATTGCC ATATTCCTGCTTCGGCACGGGGCCTATTTCTGCTCCTACATCCTATTGGATAGCCCTGACCCAAGCAAAC ATCTGCTCAGGAAGTATTTCATCGAGGCCAGCGCCCTGCCCAGCAGCTGTCCCGGGAAAACCGCACTTTG TGTGAAATGGTCCCATCTTAAGTTGCCCTGGGTGGACCTCGACTGGCTCTTAGACATCTCCTGTCAGATC ACAGAGCTTGACCTCTCTGCCAACTGCCTGTCTTCTCTCCCCTCCATCATTCCTTGGGGACTGATCAATC TGAAGAAGCTGAACCTCTCAAACAACCACCTGGGGGAGCTGCCCTGTGTGCAATCATCGGACGAAATCAT CTGCTCCAGGTTAGTTGAAATCGACATCTCCAGTAACAAACTGTCCCACCTCCCGCCTGGATTCTTGCAC CTCTCAAAACTTGAAAGGCTGACTGCTTCCAAAAACTATCTGGAGCGGTTGTTTGAAGAAGAAAATGCCA CAAACTGGATCGGCCTGCGGAAGCTGGAGGAACTCGACCTAGCTGACAACAGACTGACGGAGCTCCCTGT CCAATTTATGCACTCCTTCAAGTCTCTCACCAATCTGAATGTCTCCAGGAACAGCCTCAAGAGCTTTCCA GACCCCTGGTCCTGCCCTCTGAAATGTTGTAAAGCCTCCAAAAATGCTCTGGAATCGCTGCCAGACAAAA TGGCCGTCTTCTGGAAAAACCACCTCAGGGACGTGGATTTCTCCGAGAACTCTCTGAAAGCGGTGCCCCT GGGGGTTTTCCAACTTGATGCCCTCATGTTCTTGAGGTTACAGGGAAACCTGCTGTTGTCACTGCCACAT CATGAGAAGTGGACCTGCAGACAACTCAAGACCCTGGACCTCTCCAGAAACCAACTTGGCAAAAATGAAG ATGGGCTTAAAACAAAGCGGATCTCCCTTTTTACCACCAGAGGGCGCCAGCGTTCTGGAACTGAGACAGC GTCCGTGCTAGAATTCCCAGCTTTTCTAAGCGAGTCTTTGGAGGTCCTTTGTCTGAATGACAACCATCTT GATGCAGTTCCTCCGTCAGTCTGCCTGCTGAAGAACCTCTCAGAGCTCTACTTGGGCAATAACCCTGGTC TCCGAGAGCTCCCTCCGGAGCTAGGCCAGCTGGGAAACCTCTGGCAACTGGACATTGAAGATCTGAACAT TGGCAACGTGCCTGCAGAGGTCAGGAAAGAAGGCCCAAAAGCAACGCTGTCTTTCCTGCGTGCTCAGCTG CGCAAAGCAGAGAAGTGTAAACTGATGAAGATGATCCTCGTGGGCCCCCCACGCCAGGGCAAGTCCACAC TCCTGGAGATCTTACAGACAGGGAAGGCCCCGCAGCCAGCACACAGCGAAGCCACTGTCAGGACCACCAA GTGGGAGCTCCAGAGACCAGCAGGCTCCAAAGCCAAGGTTGAGTCTGTGGAGTTCAACGTCTGGGACATC GGGGGCCCTGCCAGCATGGCCACAGTCAATCAGTGCTTCTTCACAGATAAGGCCCTATATGTGGTGGTCT GGAACCTGGCCCTGGGGGAAGAAGCTGTGGCCAACCTCCAGTTCTGGCTTCTCAACATTGAGGCCAAGGC CCCAAACGCCGTTGTGCTGGTGGTTGGGACACATCTGGACCTTATCGAAGCTAAATTCCGAGTAGAGAGG ATCGCAACACTGCGTGCCTACGTGCTGGCCCTCTGCCGCTCACCATCGGGGTCCAGAGCTACAGGATTCC CAGACATCACTTTCAAACACTTGCACGAGATCTCCTGCAAGAATCTGGAAGGGCAGGAGGGGCTGAGGCA ACTGATCTTCCATGTCACGTGCAACATGAAGGACGTGGGCAGCACTATCGGCTGTCAAAAACTCGCTGGC AGGCTGATCCCCAGGAGCTACATAAGCCTACAGGAGGCTGTGCTGGCTGAACAACAGCGCCGCAGCCTGG AAGATCAAGTACAGTACCTGACGGACAGGCAGCTGGAGCAGCTGGTGGAGCAGACGCCTGGCAATGACAT CAAAGACTATGAGGACCTGCAGTCTGCCATCAGCTTCCTCATAGAAACCGGAACCCTGCTGCACTTCCCA GACACGAGCCATGGCCTAAGGAATCTCTACTTCCTGGATCCCATCTGGCTCTCTGAATGCCTACAGAGGA TCTTTAATATCAAGGGCTCGCGGTCGGTGGCGAAGAATGGGGTGATCCGAGCAGAGGACCTCAGGATGCT GCTGGTGGGGACAGGCTTCACGCAGCAGACGGAGGAGCAATACTTCCAGTTCCTTGCCAAGTTCGAGATT GCCCTTCCGGTTGCCAATGACAGTTACCTCCTACCACACCTCCTCCCATCCAAACCTGGGCTGGACACCC ACAGCATGCGGCACCCAATGGCTAACACCATCCAGCGGGTGTTTAAGATGAGCTTCGTGCCTGTTGGCTT CTGGCAAAGGTTCATAGCACGGATGCTGATCAGCTTGGCTGAGATGGACCTGCAGCTTTTTGAAAACAAG AAGAATACGAAAAGCAGGAACCGGAAAGTCACCATTTACAGCTTTACAGGGAACCAGAGAAACCGCTGCA GCACATTCAGAGTCCGAAGGAATCAGACCATCTACTGGCAGGAAGGGCTGCTGGTCACTTTCGATGGGGG CTACCTCAGTGTGGAATCCTCAGACGTGAACTGGAAAAAGAAAAAAAGCGGAGGGATTAAAATCGTCTGC CAGTCAGAAATGAGGGACTTCTCAGCAATGGCTTTTATCACAGACCACGTCAACTCCCTGATCGACCAGT GGTTCCCCGCCCTGACAGCCACAGAAAGTGACGGGACCCCACTTATGGAGCAGTATGTGCCCTGCCCCGT CTGTGAAGCATCCTGGGCCCAGCATGCAGATCCAAACGAGAGGTCGGAGAGCGTGCAGTACTTCGATATG GAAGATTGTGTGCTCACAGCCATTGAAGGGAACTTCATCTCCTGCCCCAGACACCCAGACCTCCCAGTGC CACTTCAGGAGCTTGTACCAGAGCTGTTTATGACTGACTTCCCAGCCAGGCTTTTCCTGGAGAACAGCAA GCTGGAGCACACAGAGGGTGAGAACAGCATCCTGGGCCAAGGTGGCAGTGGCACAGTCATCTACCAGGCC CAGTACCAGGGCCAGCCTGTGGCTGTGAAGAGATTCCACATCAAGAAGTTCAAGAATTCTGCTAATGCCC CGGCAGACACCATGCTGAGGCACCTGAGAGCCATGGATGCCATGAAGAACTTCTCGGATTTCCGTCAGGA GGCCAGCATGCTGCATGCCTTGCAGCACCCCTGCATTGTGTCACTCATCGGCATCAGCATCCACCCCCTC TGCTTCGCCCTGGAGCTCGCCCCGCTGGGCAGCCTCAACACTGTGCTGTCTGAGAATGCCAAAGATTCTT CCTTTATGCCCCTGGGACACATGCTCACCCAAAAAATAGCCTACCAGATTGCCTCGGGCCTGGCCTACCT GCACAAGAAGAACATCATCTTCTGTGACCTCAAGTCAGACAACATCCTGGTGTGGTCACTGAGCGTCAAG GAGCTCATCAACATCAAACTGTCAGACTACGGCATCTCGAGGCAGTCCTTTCACGAGGGGGCCCTGGGTG TGGAGGGCACCCCAGGCTACCAGGCTCCAGAGATCAGGCCTCGCATTGTGTATGACGAGAAGGTAGACAT GTTCTCCTATGGCATGGTGCTGTATGAGCTGCTCTCAGGACAGCGTCCTGCGCTGGGCCACCACCAACTT CAGATTGTCAAGAAGTTGTCCAAGGGCATCCGCCCAGTGCTGGGGCAGCCAGAGGAAGTGCAATTCTATC GACTGCAGGCGCTCATGATGGAGTGCTGGGACACGAAGCCGGAGAAGCGTCCCCTGGCCCTGTCTGTGGT GAGCCAGATGAAAGACCCAACCTTCGCCACCTTCATGTACATGCTGCCCTGTGGGAAGCAGTCGTCCTTC TTCTCATCCCAGAGCCAGGAGTACACTGTGGTGTTCTGGGATGGGAAGGAGGAGTCAAGGAATTACACGG TGGTCAACACCGAGAAGGGCCTTCTGGAAGTACAGAGGATGACCTGTCCTGGGATGAAGCTGAGCTGTCA GCTCAAGGTCCAGAGCTCAGTGTGGCTAGCCACCGAGGACCAGAAAATCTATATCTACAGCCTTAAGGGT ATGTGCCCATTAAGCATGCCCCAGCAGGCCCTGGACACGCCGGCTGTTGTCACCTGTTTCTTGGCAGTAC CTGTTATTAAAAAGAACTCCTTTCTGGTGCTGGCTGGCCTGGCTGATGGACTCGTGGCTGTGTTTTCTAT GGCACGGGGCACCCCAAAAGAAAGCTGCTCCTACCTGTGCTCGCACACAGCCAACAGGTCCAAGTTCTGC ATCCCAGACGAAGACTCACGGCAGAACCCCTACCCAGTGAGGGCAATGGAAGTGGTCAACAGCGGGTCTG AGGTCTGGTACAGCAATGGGCCAGGCCTCCTCATCATCGACTGTGCCATCCTGGACATCAGCAGGCGGCT GGAGCCCTATGCAGCCCCATCTGTGGTCACATCACTTGTGTGCAGCTCAGACTGCAGAGGAGAGGAGACA GTCTGGTGCCTGGACGACAAGGGCAATTCCTTAGTGATGTACCATTCAGCCACCTACCAGCTGTGTGCCC GGTACTTCTGCGGGGACCCCAGTCCTCTCAGGGACACGTTTTCTGTACAGCCCTCAGTCCCAGAAAGCCC AATCCGTCACATAACCACCTCAAAGGAGCCTGAGGAAGAGTGCATCGCCGACGTGAGCATCATGTACAGC GAGGAGCTGGGCATGCAGATCCTCACCCACCAGGACTCGCTCACTGACTACTGCTCCGTATCCTCGTACT CATCCACACCCCACCCAGACCCCAAGTCCCCCACCAGCCTGCCCAGCTCCCTCACCAGCTATTCCAGTGT GCCTTTCTCCGCCAATTATGAAGACCCAGACAGGCTGCAGGAGCCCAGCGTCACCCCTGACAGAACTGAG CATGATCTGAGCCCCATGGATGGGGAGACGTTCAGCCAACACCTGCAGGCTGTGAAGGTGCTTGCTGTCC AAGACCTCATTTGGGTCCCTAGACATGGTGGAGATATCATTGTCATCGGCCTGGAGAAGGATTCGGGTGC CCAGCGGGGCAGGGTCATCGCTGTTTTAAAAGCCAGAGAGCTCAATCCGCATGGGGTCCTGGTGGATGCG GCAGTGGTGGCAAAGGACACCGTAGTGTGCGGCTTTGCAAACGAAAACACAGAGTGGTGCCTGGCTGTCT GGAGGGGCTGGGGCGCCAGGGAGTTTGACATCTTCTACCAGTCCTATGAGGAGCTGGGCCGGCTGGAGGC GTGTCCTCGCAAGAGAAGGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001191624
Insert Size	6042 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001191624.1, NP_001178553.1
RefSeq Size	6445 bp
RefSeq ORF	6042 bp
Locus ID	308703

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