Sec16a (NM_001276417) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217765 representing NM_001276417 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCACCACCTCAGGCAGTCCCGTCTGGTGTGGCTGGCCCACCCCCAGCTGGGAATCCTCGAAGCT CGTTCTGGGCCAACAGCCCTTACAGGAAGCCAGCCAATAATAATGCACCAGTGGCTCCCATAACACGCCC ACTGCAGCCAGTAACGGATCCGTTTGCTTTTAACAGACAGACTCTTCAAAACACACCAGTGGGCAGTTCA TCCAAAAGCAGCCCACCTAATTTGCCTGGCCCAGCCCTCCCTGTTTTTTCTCAGTGGCCTGGTTTGCCTG TGCCTCCCACAAATGCTGGGGATAGCTCCACAGGACTTCATGAGCCTCTGCCAGGAACTCTGTCACAGCC TAGAGCAGACCCCAGTCTATTTCCTCCTGCATCTACCCCTTCTTCATTGCCTGGGCTGGAGGTGAACAGG AGTGCTGAGGTTGATTCCAGCTCAGGACCTGAAGTCCAGATGCTGCCACATCCACCTCACTACATTCCAG GAGTGGGTCCTGAGCAGCCTCACGGAGGCCATCTGAATGACAGTGGGGCTGGGCCTGACCAATCCATGAA TAGGCACGCCCCACATGATGGTGCTGTGGCCCATGCAGCATCTCCTTTCTTCCCTCAGCCCCAGATGCCA GGTCAGTGGGGGCCTGTGCATGGAGGCCCTCCGCCCTCCTATCATCATCACTCACCCTGCCTGGAGGGCC CTGTTCAGGACATGGGGCCCCAGGCTGCCAGCTTGCCCCATTTCCCCTCTCCATCTAGTCTACACGAGGG ACCTGGCCATGAGTCCCGTGTCCCACAAACGTTTACTCCAGCATCCTTGGCCAGTGGTGAAGGAAATGAG ATAGTCCACCAGCAAAGTAAAAATTACCCATCGAGTAGCTTCCCCCCTAAACACAGTTTTGAGCAGAATT CCAGAGTTGGGAATATGTGGGCAAGCGCGGAGTTCAGGCAGAATCCAGGAGTTAATAAAGAGCATCTGCT AGACCCTGCCCATGTGAATCCCTTCACTCAGGGAAACAGCCCTGAGAACCAGGTGCACCATCCCCCAGTG GCCGCAACTAACCATGCCCTGCAAGAAGCAGCCTCCGGAGCGCTCTCAATGTTTTTTCAAGGGGAGGAGA CAGAGAACGAGGAAAATCTCTCATCTGAAAAAGCAGGCCTTGATGACAGATTAGACTCGGGTAGCTTCTC TTCTACCTCCAGACTGGGCCATCCACCACTCCCTGGAGCTGGTGGTGTCTACCAGGCCGCTCAGGAAGGA GATGTGCAACCTTATTTTTCTCAGTTTGTGGGCATCCGTCATGACAAACAGACCACTATGACTCCTGCTA ATGATGCGTGGGGTGATATTCCGGGTGCTGGGACCCACTGTGCTAGTGGGCCACAGTGTGAGAATGTAGA GAACCTAGAATTTGTTCAGAATCAAGAAGTTTTGCCACGTGAGACCGTGAATGTGGATCCCTTTTCCCCG AGTGATCAGTTTCGATATGGGCCCCTTCCTGGGCCAGCTGCCTCCAGGCCTGTTACTTCGGGCCTCACTA GAGGAGGAGGGCTAAATCTTGAGGCCCCTGACATGCCACTGCACCCTACACGACCTGACAGTGTGTCATC CAGCTACAGCAACCATAGTCACAGAAGTCCTCCTGGGTCAGCCAGACCCCAAGAATTGGTGGGCACATTC ATTCAGCAAGAAGTTGGAAAACTCGAAGATGATACGTCAGGGAGTTTTTTTAAGCAAATTGATTCTTCTC CGGTTGGAGGTGAAACAGATGAGATGACCAGGAGTCAAAATTACTGCAGCAGCCTGTCCCAGCCTTCAAC TCCAAGCCCTCCAAAACCTACGGGAGTCTTCCAGACAAGTGCAAATAGTTCTTTTGAGCCAGTAAAATCC CACTTGGTTGGAGTGAAACCAGTTGAAGCAGATCGTGCCAACATGGTGGTTGAAGTGAGAGGGACCCAGA ACTGCCAGAAGCACGGGGCCGCTGTTCTACCACCTGATGCCACCCCAGGAAACCTGGAACAGCCACCAGA CAACATGGAGACCCTGTGTGCACCTCAGGCCTGCCCTTTGCCTCTTAGCACCACTGGAGAGGCTGGGCAA CTGGTTTCACACACAGCAGGGCCACCCTTGGATACTGTGCGCCCCATGCCTGACAAGAGGTCCTCAGCCA GGGCCCAGGGGCCTGTGAAATGTGAGAGCCCAGCCACAACTCTGTGGGCACAAAACGAACTGCCAGATTT TGGGGGCAATGTCCTTCTAGCCCCTGCTGCCCCTGCACTTTATGTTCCTGTAAAACCAAAACCTTCAGAG GTTGTTCATCATCCAGAAAAGGGCATGTCTGGACAGCAGTCATGGAAATCAGGGTCTGTGCCCCCTTTGC AGAACCAAGACCCTCCTGGTGCTTCTGAGAACCTTGAGAACCCTCCCAAGGTAGGAGAAGAGGAGGCCCT TCCATTGCAGGCAAGTTCTGGTTATGCGAGTTTGCTGTCCTCACCACCCACTGAATCTTTACACAATCAA CCTGTCCTGATTGCCCAGCCTGATCAAAGCTATAATTTGGCTCAGCCCATTAATTTTTCTGTGTCCTTAC CGAATCCTAATGAGAAGAATCAGTGCTGGGGAGATGCTGTGGTGGGGGAGAAATCCATGGTGAGCACCCG GGCTCTTGGTGCTGACCATGAAGAACATGCTGCCTTGTCTGCAATTCCAGCCAGTACTGTCACTGGCTCG TCTTTGCCTAACAGTGTCACCCAGAACTGTGCCCCTCGAGGTTCTGGGTCTTCTGAAATGATTGCTAGCC AGCCTGCCAGTTGGTTGGTTCAACAGCTGTCCCCTCAGATTCCTCAAAGTCAGCACCCCAATGCAGAGAA GGGTCCTTCTGAGTTTGTTAATAGCCCTGCTAATGGAAGTGTGATGCTAGCTCCACCTGCAAACAGTGCT GTGATACCTAACAGTAATAAGGGGTATCACTCTAGAAACCCGGAAGAAGCCATCGGTGCCCTAGACTTCA CATTGAGTAGAACTTTGGAAAATCCTGTGAGAATGTGTAGCCCATCTCATTCAAACAGCCCAGCTTCTCA GCAGCCTCTTGCCAATCATCCCAGACAATCTGGGCCTGGGCTGCATAACCAGGATCATTTCTACCAGCAG GTAACAAAAGATGCTCAGGATCACCGTAGCCTAGAAAGAGGCCAGTCAGAGCTGGTGCCTCCTCCGCCAC AGAATTCTCCCCAACAAGTACCCCAAGCTGCGTGTCCAGAGCCTTCAAATCCAGAAAGCCCACCCACACA AGGACAGTCTGAAGGCTTGGTCCAGCCATCAGCAAGTCCAGCTCCAGTCAACACAGGCCAGCAGCCTCAG CCACCGCAGTCCTCCAGTGCATCGGTTACATCTACCAACTCCAACCAGGCAGCTGTGCGGTCAGAACAGC CATGGCTACACCCACCTCCCACTACTTTTGGCCCAGCACCTCAAGACTTGGCATCCTACTACTATTATAG ACCCCCGTATGAAGCCTACCAGTCTCCGTACCCTTCACCGTACCCATCAGATCCTGGAGCAGCCTCTTTA TACTACCAAGATATCTATGGCCTGTATGAGCCTCGTTACAGACCCTATGACAGCTCGGCATCTGCTTATG CCGAGAACCACCGCTACTCTGAACCTGAGCGGCCCAGCTCCCGTGCAAGTCACTATTCTGACCAGCTCAC TCCCAGGCAAGGATATCCTGAAGGATACTACAATTCTAAGAGTGGATGGAGCAGTCACAGTGACTACTAC GCAAATTACTACTCTGGCCAGTATGATTATGGAGACCCAGGCCGCTGGGACCGTTACTATGGGTCTCGAT TTAGGGACCCTCGCACCTGGGACCGGAGGTACTGGTATGACTCTGACCATGACCCATACAGGAAGGACAA CTATGCTTACAGTGACAGGCCTGAGAAATGTGATGATCACTGGAGGTATGACCCTCGCTTCACTGGGAGC TTCGATGATGACACTGAGCTCCACAGGGACCCCTATGGAGAAGAAGTAGACAGACGCAGCATCCACAGCG AGCACTCAGCCCGGAGCCTGCGCAGTACTCACAGCCTGCCCAGCCGCCGCAGCAGCCTCAGCTCCCATTC ACACCAGAGTCAGATTTACAGAAGCCACCATGTGACTGGCGGTTCCTTTGAGGCCCCCCATGCCCCAGGC TCATTTCATGGTGATTATGCCTATGGTACCTATGCCAGCAATTTCAGCGGTGCCCATGGTTTTCCAGAGT ACAGCTACCCTGCAGACACCTCCTGGCCTGCTGCGGAGCAAGTTCCATCAAGACCGACCTCTCCTGAGAA GTTTACAGTGCCTCACGTCTGTGCCAGGTTTGGTCCTGGTGGTCAGCTCCTCAAAGTGATACCCAACCTG CCTTCAGAAGGACAGCCCGCATTGGTGGAGATCCACAGCTTAGAGACCTTGCTGCAGCACACACCTGAGC AGGAAGAAATGCGCTCCTTCCCAGGACCTCTCGGCAAAGATGACACTCACAAAGTAGATGTTATTAACTT TGCACAAAACAAAGCGACAAAATGTTTGCAGAATGAAAGTTTAATTGACAAAGAGTCTGCAAGTCTTCTT TGGAACTTCATTATTCTGTTATGCAGACAGAATGGGACCGTCGTGGGAACAGACATCGCAGAGCTCTTGT TACGGGACCATAGAACTGTGTGGCTTCCTGGGAAGTCACCCAACGAGGCAAACCTGATAGATTTTACCAA TGAGGCTGTGGAGCAAGTGGAAGAGGAGGAGTCAGGGGAGGCCCAGCTCTCATTTCTCACAGACAGTCAG ACAGTGACCAGCAGTGTGCTGGAGAAGGAGACTGAGCGGTTCCGTGAACTCTTGCTGTACGGTCGGAAGA AGGATGCTCTGGAGTCTGCCATGAAAAATGGCTTATGGGGTCACGCCCTGCTGCTTGCAAGTAAGATGGA CAGCCGGACACATGCCCGTGTCATGACCAGGTTTGCCAACAGTCTTCCGATCAATGACCCCCTGCAGACT GTCTACCAGCTCATGTCTGGACGGATGCCTGCTGCATCCACGTGTTGTGGAGATGAGAAATGGGGAGATT GGAGGCCACATCTTGCAATGGTTTTGTCCAACCTGAACAACAACATGGATGTGGAATCTCGGACCATGGC TACCATGGGTGATACCCTAGCATCAAAAGGCCTGCTGGATGCCGCACATTTTTGCTACCTCATGGCCCAG GTTGGATTTGGGGTTTATACAAAGAAAACTACCAAACTTGTTTTGATAGGCTCAAATCACAGTTTGCCAT TTTTAAAGTTTGCCACTAATGAAGCCATTCAAAGGACGGAGGCTTATGAGTACGCTCAGTCCCTTGGGGC GCATACCTGTTCCTTACCTAACTTCCAGGTGTTTAAATTCATCTACTTGTGCCGCCTGGCTGAAATGGGA CTCGCTACACAGGCCTTCCACTACTGTGAAGTAATAGCCAAGAGTGTCCTGACACAGCCTACTGCATACT CCCCAGTGCTGATTAGCCAGTTGATTCAGATGGCTTCCCAGTTACGCCTCTTCGATCCTCAACTGAAGGA GAAGCCAGAGGAGGAGTCCTTTGTGGAGCCTGCCTGGTTGGTGCAGCTGCAGCACGTGGAGAGGCAGATC CAGGAGGGCACTGTGCTGTGGAACCAGGATGGAACTGAACCCCAGCAGTGTCACATCACATCGGGCTCTG AGGTGGAGCAGCCAGATGGCCCTGGACTCAACCAGCAGGCAGGGCCAAGGGCCGACAACCCTCTCCTGAT GCCAAGCACTGAGCCTTTGGTGCACAGCGTGCAGCTGCTGCCCACAGCTCCTCAGACATTGCCTGATGGC CAGCCTGCTCACCTTGCCAGGGTGCCCATGTTCCCAGTACCAATGTCTCGTCCCCCCCTGGAGCTGAGTG CTGCCTATGGACCCCCGGGGTCTGTACTTGGCTTCCCAGAATCCTCCAGATCTGATCCTGCAGTGCTGCA TCCTGGGCAGGCCCTACCACCCGCTACACCAGGTCTCCAGGAAAGTGGGCCACCACTCCAGGAGGCCAGA AGCCCAGATCCAGAAATGGTGCCACAGGGCTCACCTGTCAGACACTCCCCTCCTGAGCCCCGTGAAGAGG AGTTTGGCAAGAACGTCGCTGACCTGGGCTCCTCTAGAACAGCACAGGACTCGGAAACCTCCCCAGTGTG GGATCTTGGCAGCTCCAGTCTGACACGTGCCCCATCTTTGACACCTGATTCTGAAGGGAAGAAACCTGCA CAAGCTGTCAAAAAGGAGCCCAAGGAGCCCAAGAAGACCGAGTCCTGGTTCTCTCGCTGGCTGCCTGGGA AGAAAAGGACAGAAGCTTATCTACCAGATGACAAGAACAAATCAATTGTTTGGGATGAAAAGAAAAACCA GTGGGTGAATCTGAATGAACCGGAGGAGGAGAAGAAGGCTCCACCCCCACCTCCAACATCGTTCCCCAGG GTTCCCCAGGCGGCTCCCACTGGGCCTGCAGGACCACCCATGGCCTCCGTGAATGTGTTTTCTAGAAAAG CAGGTGGGTCCAGAGCTCGCTATGTGGATGTTCTCAACCCAAGTGGAACACAGCGGAGTGAACCAGCTCT TGCTCCTGCGGATTTCTTTGCTCCTCTTGCCCCGCTGCCAATCCCTTCTAACTTATTTGTACCAAACCCA GATGCAGAAGAGTCACAACCTGCAGATGGGACTGGCTGTAGGGGACAGGCACCAGCTGGGACTCAATCTA AGGCAGAGCCCACCCTGGAAGCCAAGGTGGTAAGCTCTACAGTATCAGCCCCTGGGCCTGAACTCTCACC CTCCAAACCAGCAGATGGCTCCCAGGGAGGAGAGGCTCCTGGAGACCACTGTCCTGCAGGGGCCACTCCT GGTGGGTCTGTGCCCTTTTACAACCCTGCGCAGTTGGTGCAGGCGTCTGTCACCTCGGGGACTTCAAGGC CAGGAAGGATTGGCCAAAGAAAATATGCGGCACTGAACTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276417
Insert Size	7041 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276417.1, NP_001263346.1
RefSeq Size	8889 bp
RefSeq ORF	7041 bp
Locus ID	100360302

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