Itpr1 (NM_001270597) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	I145TR; InsP3R; InsP3R1; IP3R1; P400
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217786 representing NM_001270597 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACAAAATGTCTAGTTTCCTACATATCGGAGACATTTGTTCTCTGTATGCAGAGGGATCTACGA ATGGATTTATCAGCACCTTGGGCTTGGTTGATGACCGTTGCGTTGTACAGCCAGAAGCTGGGGACCTTAA CAATCCACCCAAGAAATTCAGAGACTGCCTCTTTAAGCTATGTCCTATGAATCGATATTCTGCACAGAAG CAGTTCTGGAAAGCTGCTAAGCCCGGGGCCAACAGTACAACAGATGCGGTGCTGCTCAACAAATTGCATC ATGCTGCAGACTTGGAAAAGAAGCAGAATGAGACAGAAAACAGGAAATTGCTGGGGACGGTCATCCAGTA TGGCAACGTGATCCAGCTCCTACATTTGAAAAGCAATAAATACTTAACTGTGAATAAGAGGCTTCCTGCC CTGCTGGAGAAGAACGCCATGAGAGTGACGTTGGATGAGGCTGGAAATGAAGGGTCTTGGTTTTACATTC AGCCGTTCTACAAGCTCCGCTCCATCGGAGACAGTGTGGTCATAGGTGACAAGGTAGTTTTGAACCCTGT CAATGCTGGGCAGCCGCTACATGCCAGCAGCCATCAGCTGGTGGATAACCCGGGCTGCAATGAGGTCAAC TCCGTCAACTGTAACACAAGCTGGAAAATAGTGCTTTTCATGAAATGGAGTGATAACAAAGATGACATTC TAAAAGGAGGTGATGTGGTAAGACTCTTCCATGCTGAGCAAGAGAAGTTTCTCACGTGCGATGAGCACAG GAAGAAGCAGCACGTCTTCCTGAGGACAACCGGCAGACAGTCAGCCACGTCGGCCACCAGCTCCAAAGCC CTGTGGGAAGTGGAGGTAGTCCAGCATGACCCATGTCGGGGTGGAGCTGGGTACTGGAATAGCCTCTTCC GGTTCAAGCACCTGGCTACAGGGCATTACTTGGCAGCAGAGGTAGACCCTGACTTTGAGGAAGAATGCCT GGAGTTTCAGCCCTCAGTGGACCCTGATCAGGACGCATCTCGGAGCAGGTTGAGAAATGCCCAAGAAAAA ATGGTGTACTCTCTGGTCTCTGTGCCTGAGGGCAACGACATCTCCTCCATCTTTGAGCTAGACCCCACAA CTCTACGTGGAGGTGACAGCCTTGTCCCAAGGAACTCCTATGTCCGGCTCAGACACCTGTGTACAAACAC CTGGGTTCACAGCACAAACATCCCCATTGACAAGGAAGAGGAAAAACCTGTCATGCTGAAGATTGGTACC TCTCCCCTGAAGGAGGACAAGGAAGCTTTTGCCATAGTCCCCGTTTCTCCTGCTGAGGTTCGGGACCTGG ACTTTGCCAATGATGCCAGCAAGGTGCTGGGCTCCATCGCTGGGAAGTTGGAAAAGGGCACCATCACCCA GAATGAGAGAAGGTCTGTCACCAAGCTGCTGGAAGACTTGGTTTACTTTGTCACGGGTGGAACGAACTCT GGCCAAGACGTGCTTGAAGTAGTCTTCTCTAAGCCCAACCGAGAGCGGCAGAAGCTGATGAGGGAACAGA ATATTCTCAAGCAGATCTTCAAGCTTTTGCAGGCCCCCTTCACGGACTGCGGGGATGGCCCAATGCTTCG GCTGGAGGAGCTGGGGGACCAGCGGCACGCTCCTTTCAGACACATTTGCCGACTCTGCTACAGGGTCCTG AGACACTCACAGCAAGACTACAGGAAGAACCAGGAGTACATAGCCAAGCAGTTTGGCTTCATGCAGAAGC AGATTGGCTATGATGTGCTGGCCGAAGACACCATCACTGCCCTGCTCCACAACAACCGAAAGCTCCTGGA AAAGCACATCACCGCAGCAGAGATTGACACGTTTGTCAGCCTGGTGCGAAAGAACAGGGAGCCCAGGTTC TTGGATTACCTCTCTGACCTCTGTGTATCCATGAATAAGTCAATCCCTGTGACCCAGGAGCTCATTTGTA AAGCTGTGCTGAATCCCACCAATGCTGACATCCTGATTGAGACCAAGTTGGTTCTTTCTCGTTTTGAGTT TGAAGGCGTTTCCACCGGAGAGAATGCTCTGGAAGCTGGGGAAGATGAGGAAGAGGTGTGGCTGTTCTGG AGGGACAGCAACAAAGAGATCCGTAGCAAGAGTGTTAGGGAATTGGCACAAGATGCTAAAGAGGGACAGA AGGAAGACAGGGATGTCCTCAGCTACTACAGGTATCAGCTGAACCTCTTTGCAAGGATGTGTCTGGACCG CCAGTACCTGGCCATCAATGAAATATCCGGGCAGCTGGATGTTGACCTCATCCTCCGCTGCATGTCCGAT GAGAACCTCCCCTATGACCTCAGAGCATCCTTTTGCCGCCTCATGCTTCACATGCATGTGGACCGAGATC CCCAGGAGCAGGTGACCCCTGTGAAATACGCCCGTCTGTGGTCAGAAATTCCCTCCGAGATCGCCATTGA CGACTATGACAGCAGTGGAGCATCGAAGGATGAAATCAAGGAGAGGTTTGCTCAGACGATGGAGTTTGTG GAGGAGTACCTGAGAGATGTGGTTTGTCAAAGATTCCCCTTTTCTGATAAGGAGAAAAATAAGCTCACAT TTGAGGTTGTGAACTTAGCCAGGAACCTCATATACTTTGGCTTCTACAACTTTTCTGACCTTCTCCGATT AACCAAGATCCTCTTGGCAATCTTAGACTGTGTTCATGTGACAACCATCTTCCCCATTAGCAAGATGACG AAAGGCGAAGAGAATAAAGGCAGCAACGTGATGAGATCTATCCATGGAGTTGGGGAGCTCATGACCCAGG TGGTGCTTCGGGGAGGAGGCTTTTTGCCCATGACTCCTATGGCTGCTGCCCCTGAAGGAAATGTGAAGCA GGCAGAGCCAGAGAAAGAGGATATCATGGTCATGGACACCAAGTTAAAGATCATTGAAATACTCCAGTTT ATTTTGAATGTGAGATTGGATTATAGGATCTCCTGCCTCCTGTGTATATTTAAGCGGGAGTTTGATGAAA GCAATTCCCAGTCATCAGAAACATCCTCCGGAAACAGCAGCCAAGAAGGGCCAAGTAATGTACCAGGTGC TCTTGACTTTGAACACATTGAAGAGCAAGCAGAAGGCATCTTTGGGGGAAGTGAGGAGAACACACCTCTG GACTTGGATGATCATGGTGGCAGAACCTTCCTCAGGGTCCTGCTCCACTTGACAATGCATGATTACCCAC CCCTGGTGTCTGGGGCCCTGCAGCTCCTCTTCCGGCATTTCAGCCAGAGGCAGGAGGTCCTTCAGGCCTT CAAACAGGTTCAACTGCTGGTTACTAGCCAAGATGTGGACAACTACAAACAGATCAAGCAAGACTTGGAC CAGCTAAGATCCATCGTGGAGAAGTCTGAGCTCTGGGTATACAAAGGCCAGGGTCCCGATGAGCCTATGG ACGGAGCCTCTGGTGAAAACGAACATAAGAAAACTGAGGAGGGGACCAGTAAGCCACTGAAGCACGAAAG CACCAGCAGCTACAACTACCGAGTGGTGAAAGAGATTCTGATTCGACTTAGCAAGCTCTGTGTTCAAGAG AGCGCGTCGGTGAGGAAGAGCAGGAAGCAGCAGCAGCGACTGCTGAGGAACATGGGTGCCCATGCTGTGG TGCTGGAGCTGCTGCAGATCCCCTATGAGAAGGCCGAAGATACCAAGATGCAGGAGATCATGAGGCTGGC TCACGAATTTCTGCAGAATTTCTGCGCAGGCAACCAGCAGAACCAAGCTTTGCTACATAAACACATAAAC CTGTTTCTCAACCCAGGGATCCTGGAAGCAGTGACCATGCAGCACATCTTCATGAACAACTTCCAGCTGT GCAGTGAGATCAACGAGAGAGTGGTCCAGCATTTCGTTCACTGCATAGAGACCCACGGTCGAAATGTCCA GTATATCAAGTTTCTCCAGACTATTGTCAAGGCCGAAGGGAAATTCATTAAAAAGTGCCAAGACATGGTC ATGGCTGAGCTTGTCAATTCTGGAGAGGACGTCCTCGTGTTCTACAATGACAGAGCCTCTTTCCAGACTC TGATCCAGATGATGCGGTCCGAGCGTGACCGGATGGATGAGAACAGCCCTCTCATGTACCACATCCATCT GGTGGAGCTCTTGGCTGTGTGCACAGAGGGCAAGAATGTGTACACAGAGATCAAGTGCAACTCCCTGCTC CCGCTCGATGACATCGTTCGTGTGGTCACTCATGAAGACTGTATCCCCGAGGTTAAGATTGCTTACATCA ACTTCCTGAATCACTGCTATGTGGACACTGAGGTGGAGATGAAGGAGATTTACACAAGTAATCACATGTG GAAGTTGTTTGAGAATTTCCTCGTGGACATCTGCAGGGCCTGTAACAACACAAGTGACAGGAAGCATGCA GACTCGGTTCTGGAGAAGTACGTCACTGAAATCGTGATGAGCATTGTCACCACCTTCTTCAGCTCTCCCT TCTCAGACCAGAGTACCACTCTGCAGACTCGCCAGCCTGTCTTTGTGCAGCTCCTGCAAGGCGTGTTCCG AGTTTACCACTGCAACTGGTTGATGCCGAGCCAGAAAGCCTCGGTGGAGAGCTGTATCCGCGTGCTCTCT GACGTAGCCAAGAGCCGGGCCATAGCCATTCCTGTTGACCTAGACAGCCAAGTCAACAACCTCTTCCTGA AGTCCCACAACATTGTGCAGAAAACAGCCATGAACTGGCGGTTATCAGCCCGCAACGCTGCTCGTAGAGA CTCTGTCCTGGCAGCTTCCAGAGACTACCGAAATATTATTGAGAGGTTACAGGACATCGTGTCTGCCCTG GAGGACCGACTCAGGCCCCTGGTCCAGGCCGAGCTGTCTGTGCTCGTGGATGTTCTACACAGACCGGAAC TGCTTTTCCCTGAGAACACGGATGCCAGGAGGAAATGCGAAAGTGGAGGTTTCATCTGCAAGCTCATAAA ACACACCAAGCAGCTGCTAGAGGAGAATGAAGAGAAACTGTGCATTAAAGTCTTACAGACCCTCAGGGAA ATGATGACCAAAGATAGAGGCTATGGAGAAAAGGGTGAGGCACTCCGGCAAATTTTGGTCAACCGTTACT ATGGAAACATCAGACCTTCAGGAAGAAGAGAGAGCCTTACCAGCTTTGGCAATGGTCCACTATCACCAGG AGGACCCAGCAAGCCTGGGGGAGGAGGGGGAGGTCCTGGGTCCGGCTCCACAAGCCGGGGTGAGATGAGC CTGGCTGAGGTTCAATGTCATCTCGACAAGGAAGGCGCCTCCAATCTGGTCATCGACCTCATAATGAATG CATCCAGCGACCGAGTATTCCACGAAAGCATTCTCCTGGCCATCGCACTTCTGGAAGGAGGCAACACCAC TATCCAGCACTCATTTTTCTGCCGGCTGACGGAAGATAAGAAATCAGAGAAGTTCTTCAAGGTCTTTTAC GATCGAATGAAGGTGGCCCAGCAGGAAATCAAGGCGACAGTGACCGTGAACACCAGCGATTTGGGAAACA AAAAGAAAGATGATGAAGTGGACCGGGATGCCCCATCCCGGAAGAAAGCCAAAGAGCCCACAACACAGAT AACAGAAGAGGTCCGGGATCAGCTCTTGGAAGCATCTGCTGCCACCAGAAAAGCCTTTACCACCTTCCGG AGGGAGGCCGACCCTGATGACCATTACCAATCTGGGGAGGGCACCCAGGCTACAACGGACAAAGCCAAGG ATGACCTAGAGATGAGTGCAGTCATCACCATCATGCAGCCTATCCTGCGCTTCCTGCAGCTCCTGTGTGA AAACCACAACCGCGATCTGCAGAATTTCCTTCGTTGCCAAAATAATAAGACCAACTACAACTTGGTGTGT GAGACACTGCAGTTTCTGGACTGTATCTGTGGGAGCACAACTGGAGGCCTTGGTCTTCTCGGACTGTACA TAAATGAAAAGAATGTAGCTCTTATCAACCAAACCCTGGAGAGTCTGACTGAGTACTGTCAAGGGCCTTG CCATGAAAACCAGAACTGCATCGCCACCCACGAGTCCAATGGCATCGATATCATCACAGCCCTCATCCTT AACGATATCAACCCTCTGGGGAAGAAACGGATGGACCTGGTGTTAGAACTGAAGAACAATGCTTCAAAGC TACTCCTGGCCATCATGGAAAGCAGACACGATAGTGAAAATGCAGAGAGGATCCTGTACAACATGAGGCC CAAGGAGCTGGTGGAAGTGATCAAGAAGGCCTACATGCAAGGCGAAGTGGAGTTTGAGGATGGGGAGAAC GGTGAGGATGGAGCAGCCTCCCCCAGGAACGTGGGCCATAACATCTACATCCTCGCTCACCAGTTGGCTC GGCATAACAAAGAACTTCAAACCATGCTGAAACCTGGAGGCCAGGTGGATGGCGATGAAGCTCTGGAGTT CTATGCGAAGCACACAGCACAAATTGAGATTGTCAGACTGGACCGAACTATGGAACAGATTGTCTTCCCT GTGCCCAGCATCTGTGAATTTCTGACTAAGGAATCGAAACTTCGAATATATTACACCACAGAGAGGGATG AGCAAGGCAGCAAGATCAATGACTTCTTCCTGCGCTCCGAGGACCTCTTTAATGAGATGAACTGGCAGAA GAAACTTCGAGCCCAGCCTGTCTTGTACTGGTGCGCCCGAAACATGTCTTTCTGGAGCAGCATCTCCTTC AACCTGGCCGTCCTGATGAACCTGCTGGTGGCGTTTTTCTACCCATTTAAAGGAGTGAGAGGAGGAACAC TAGAGCCGCACTGGTCCGGCCTCCTGTGGACAGCCATGCTCATCTCTCTGGCAATTGTCATTGCGCTGCC CAAGCCCCATGGCATCCGGGCCTTAATTGCTTCTACAATCCTACGACTGATATTTTCAGTTGGATTACAG CCCACACTGTTTCTGCTGGGAGCTTTCAATGTCTGCAATAAAATCATCTTCCTGATGAGCTTTGTGGGCA ACTGTGGGACCTTCACCAGAGGCTACCGAGCCATGGTTCTGGACGTTGAGTTCCTCTATCATTTGCTGTA TCTACTGATCTGTGCCATGGGCCTCTTCGTACATGAGTTTTTCTATAGTTTGCTGCTTTTTGATTTAGTG TACAGAGAGGAGACTTTGCTTAATGTCATTAAAAGTGTCACCCGCAATGGACGGTCCATCATCTTGACAG CGGTCCTGGCTCTGATTCTGGTTTACCTGTTCTCAATTGTGGGCTACCTGTTCTTCAAGGATGACTTTAT CTTGGAAGTAGACAGGTTGCCCAATGAGACAGCTGGTCCAGAAACTGGTGAGAGTTTGGCCAACGATTTC CTGTACTCTGATGTGTGCCGGGTAGAGACGGGGGAGAACTGCACCTCTCCGGCACCCAAAGAAGAGCTGC TCCCTGTGGAAGAAACGGAACAGGATAAGGAGCACACGTGTGAGACACTGCTCATGTGCATCGTCACCGT TCTGAGTCACGGGCTGCGGAGTGGGGGCGGAGTAGGAGATGTGCTCAGGAAGCCATCCAAAGAGGAGCCT CTGTTTGCTGCAAGGGTGATCTATGACCTCCTCTTCTTCTTCATGGTCATCATCATTGTCCTGAACCTCA TTTTTGGGGTCATCATCGACACCTTTGCTGACCTGAGGAGTGAGAAGCAGAAGAAGGAAGAGATCTTAAA AACCACGTGCTTCATTTGTGGCTTGGAAAGGGACAAGTTTGACAATAAGACCGTCACCTTTGAAGAGCAC ATTAAGGAAGAACACAACATGTGGCACTATCTGTGCTTCATCGTGCTGGTGAAAGTGAAGGACTCCACCG AGTACACCGGGCCCGAGAGCTACGTGGCAGAGATGATCAGGGAAAGAAACCTTGATTGGTTCCCCAGGAT GAGAGCCATGTCCCTGGTCAGCAGTGACTCTGAAGGGGAACAGAACGAGCTGAGGAACCTGCAGGAGAAG CTGGAGTCCACCATGAAGCTGGTCACCAATCTTTCTGGCCAGCTGTCAGAACTAAAGGACCAGATGACAG AACAAAGGAAGCAGAAACAAAGAATTGGCCTTCTAGGACATCCTCCTCACATGAATGTCAACCCACAGCA GCCAGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270597
Insert Size	8130 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270597.1, NP_001257526.1
RefSeq Size	9667 bp
RefSeq ORF	8130 bp
Locus ID	25262
UniProt ID	P29994
Gene Summary	This gene belongs to the Insp3 receptor family. It encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (3) lacks three consecutive exons in the coding region, but maintains the reading frame, compared to variant 1. The resulting isoform (3) lacks an internal segment, compared to isoform 1.

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