Itpr1 (NM_001270596) Rat Untagged Clone

Specifications

Product Data
Type	Rat Untagged Clone
Tag	Tag Free
Synonyms	I145TR; InsP3R; InsP3R1; IP3R1; P400
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >RN217790 representing NM_001270596 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACAAAATGTCTAGTTTCCTACATATCGGAGACATTTGTTCTCTGTATGCAGAGGGATCTACGA ATGGATTTATCAGCACCTTGGGCTTGGTTGATGACCGTTGCGTTGTACAGCCAGAAGCTGGGGACCTTAA CAATCCACCCAAGAAATTCAGAGACTGCCTCTTTAAGCTATGTCCTATGAATCGATATTCTGCACAGAAG CAGTTCTGGAAAGCTGCTAAGCCCGGGGCCAACAGTACAACAGATGCGGTGCTGCTCAACAAATTGCATC ATGCTGCAGACTTGGAAAAGAAGCAGAATGAGACAGAAAACAGGAAATTGCTGGGGACGGTCATCCAGTA TGGCAACGTGATCCAGCTCCTACATTTGAAAAGCAATAAATACTTAACTGTGAATAAGAGGCTTCCTGCC CTGCTGGAGAAGAACGCCATGAGAGTGACGTTGGATGAGGCTGGAAATGAAGGGTCTTGGTTTTACATTC AGCCGTTCTACAAGCTCCGCTCCATCGGAGACAGTGTGGTCATAGGTGACAAGGTAGTTTTGAACCCTGT CAATGCTGGGCAGCCGCTACATGCCAGCAGCCATCAGCTGGTGGATAACCCGGGCTGCAATGAGGTCAAC TCCGTCAACTGTAACACAAGCTGGAAAATAGTGCTTTTCATGAAATGGAGTGATAACAAAGATGACATTC TAAAAGGAGGTGATGTGGTAAGACTCTTCCATGCTGAGCAAGAGAAGTTTCTCACGTGCGATGAGCACAG GAAGAAGCAGCACGTCTTCCTGAGGACAACCGGCAGACAGTCAGCCACGTCGGCCACCAGCTCCAAAGCC CTGTGGGAAGTGGAGGTAGTCCAGCATGACCCATGTCGGGGTGGAGCTGGGTACTGGAATAGCCTCTTCC GGTTCAAGCACCTGGCTACAGGGCATTACTTGGCAGCAGAGGTGGACCCTGATCAGGACGCATCTCGGAG CAGGTTGAGAAATGCCCAAGAAAAAATGGTGTACTCTCTGGTCTCTGTGCCTGAGGGCAACGACATCTCC TCCATCTTTGAGCTAGACCCCACAACTCTACGTGGAGGTGACAGCCTTGTCCCAAGGAACTCCTATGTCC GGCTCAGACACCTGTGTACAAACACCTGGGTTCACAGCACAAACATCCCCATTGACAAGGAAGAGGAAAA ACCTGTCATGCTGAAGATTGGTACCTCTCCCCTGAAGGAGGACAAGGAAGCTTTTGCCATAGTCCCCGTT TCTCCTGCTGAGGTTCGGGACCTGGACTTTGCCAATGATGCCAGCAAGGTGCTGGGCTCCATCGCTGGGA AGTTGGAAAAGGGCACCATCACCCAGAATGAGAGAAGGTCTGTCACCAAGCTGCTGGAAGACTTGGTTTA CTTTGTCACGGGTGGAACGAACTCTGGCCAAGACGTGCTTGAAGTAGTCTTCTCTAAGCCCAACCGAGAG CGGCAGAAGCTGATGAGGGAACAGAATATTCTCAAGCAGATCTTCAAGCTTTTGCAGGCCCCCTTCACGG ACTGCGGGGATGGCCCAATGCTTCGGCTGGAGGAGCTGGGGGACCAGCGGCACGCTCCTTTCAGACACAT TTGCCGACTCTGCTACAGGGTCCTGAGACACTCACAGCAAGACTACAGGAAGAACCAGGAGTACATAGCC AAGCAGTTTGGCTTCATGCAGAAGCAGATTGGCTATGATGTGCTGGCCGAAGACACCATCACTGCCCTGC TCCACAACAACCGAAAGCTCCTGGAAAAGCACATCACCGCAGCAGAGATTGACACGTTTGTCAGCCTGGT GCGAAAGAACAGGGAGCCCAGGTTCTTGGATTACCTCTCTGACCTCTGTGTATCCATGAATAAGTCAATC CCTGTGACCCAGGAGCTCATTTGTAAAGCTGTGCTGAATCCCACCAATGCTGACATCCTGATTGAGACCA AGTTGGTTCTTTCTCGTTTTGAGTTTGAAGGCGTTTCCACCGGAGAGAATGCTCTGGAAGCTGGGGAAGA TGAGGAAGAGGTGTGGCTGTTCTGGAGGGACAGCAACAAAGAGATCCGTAGCAAGAGTGTTAGGGAATTG GCACAAGATGCTAAAGAGGGACAGAAGGAAGACAGGGATGTCCTCAGCTACTACAGGTATCAGCTGAACC TCTTTGCAAGGATGTGTCTGGACCGCCAGTACCTGGCCATCAATGAAATATCCGGGCAGCTGGATGTTGA CCTCATCCTCCGCTGCATGTCCGATGAGAACCTCCCCTATGACCTCAGAGCATCCTTTTGCCGCCTCATG CTTCACATGCATGTGGACCGAGATCCCCAGGAGCAGGTGACCCCTGTGAAATACGCCCGTCTGTGGTCAG AAATTCCCTCCGAGATCGCCATTGACGACTATGACAGCAGTGGAGCATCGAAGGATGAAATCAAGGAGAG GTTTGCTCAGACGATGGAGTTTGTGGAGGAGTACCTGAGAGATGTGGTTTGTCAAAGATTCCCCTTTTCT GATAAGGAGAAAAATAAGCTCACATTTGAGGTTGTGAACTTAGCCAGGAACCTCATATACTTTGGCTTCT ACAACTTTTCTGACCTTCTCCGATTAACCAAGATCCTCTTGGCAATCTTAGACTGTGTTCATGTGACAAC CATCTTCCCCATTAGCAAGATGACGAAAGGCGAAGAGAATAAAGGCAGCAACGTGATGAGATCTATCCAT GGAGTTGGGGAGCTCATGACCCAGGTGGTGCTTCGGGGAGGAGGCTTTTTGCCCATGACTCCTATGGCTG CTGCCCCTGAAGGAAATGTGAAGCAGGCAGAGCCAGAGAAAGAGGATATCATGGTCATGGACACCAAGTT AAAGATCATTGAAATACTCCAGTTTATTTTGAATGTGAGATTGGATTATAGGATCTCCTGCCTCCTGTGT ATATTTAAGCGGGAGTTTGATGAAAGCAATTCCCAGTCATCAGAAACATCCTCCGGAAACAGCAGCCAAG AAGGGCCAAGTAATGTACCAGGTGCTCTTGACTTTGAACACATTGAAGAGCAAGCAGAAGGCATCTTTGG GGGAAGTGAGGAGAACACACCTCTGGACTTGGATGATCATGGTGGCAGAACCTTCCTCAGGGTCCTGCTC CACTTGACAATGCATGATTACCCACCCCTGGTGTCTGGGGCCCTGCAGCTCCTCTTCCGGCATTTCAGCC AGAGGCAGGAGGTCCTTCAGGCCTTCAAACAGGTTCAACTGCTGGTTACTAGCCAAGATGTGGACAACTA CAAACAGATCAAGCAAGACTTGGACCAGCTAAGATCCATCGTGGAGAAGTCTGAGCTCTGGGTATACAAA GGCCAGGGTCCCGATGAGCCTATGGACGGAGCCTCTGGTGAAAACGAACATAAGAAAACTGAGGAGGGGA CCAGTAAGCCACTGAAGCACGAAAGCACCAGCAGCTACAACTACCGAGTGGTGAAAGAGATTCTGATTCG ACTTAGCAAGCTCTGTGTTCAAGAGAGCGCGTCGGTGAGGAAGAGCAGGAAGCAGCAGCAGCGACTGCTG AGGAACATGGGTGCCCATGCTGTGGTGCTGGAGCTGCTGCAGATCCCCTATGAGAAGGCCGAAGATACCA AGATGCAGGAGATCATGAGGCTGGCTCACGAATTTCTGCAGAATTTCTGCGCAGGCAACCAGCAGAACCA AGCTTTGCTACATAAACACATAAACCTGTTTCTCAACCCAGGGATCCTGGAAGCAGTGACCATGCAGCAC ATCTTCATGAACAACTTCCAGCTGTGCAGTGAGATCAACGAGAGAGTGGTCCAGCATTTCGTTCACTGCA TAGAGACCCACGGTCGAAATGTCCAGTATATCAAGTTTCTCCAGACTATTGTCAAGGCCGAAGGGAAATT CATTAAAAAGTGCCAAGACATGGTCATGGCTGAGCTTGTCAATTCTGGAGAGGACGTCCTCGTGTTCTAC AATGACAGAGCCTCTTTCCAGACTCTGATCCAGATGATGCGGTCCGAGCGTGACCGGATGGATGAGAACA GCCCTCTCATGTACCACATCCATCTGGTGGAGCTCTTGGCTGTGTGCACAGAGGGCAAGAATGTGTACAC AGAGATCAAGTGCAACTCCCTGCTCCCGCTCGATGACATCGTTCGTGTGGTCACTCATGAAGACTGTATC CCCGAGGTTAAGATTGCTTACATCAACTTCCTGAATCACTGCTATGTGGACACTGAGGTGGAGATGAAGG AGATTTACACAAGTAATCACATGTGGAAGTTGTTTGAGAATTTCCTCGTGGACATCTGCAGGGCCTGTAA CAACACAAGTGACAGGAAGCATGCAGACTCGGTTCTGGAGAAGTACGTCACTGAAATCGTGATGAGCATT GTCACCACCTTCTTCAGCTCTCCCTTCTCAGACCAGAGTACCACTCTGCAGACTCGCCAGCCTGTCTTTG TGCAGCTCCTGCAAGGCGTGTTCCGAGTTTACCACTGCAACTGGTTGATGCCGAGCCAGAAAGCCTCGGT GGAGAGCTGTATCCGCGTGCTCTCTGACGTAGCCAAGAGCCGGGCCATAGCCATTCCTGTTGACCTAGAC AGCCAAGTCAACAACCTCTTCCTGAAGTCCCACAACATTGTGCAGAAAACAGCCATGAACTGGCGGTTAT CAGCCCGCAACGCTGCTCGTAGAGACTCTGTCCTGGCAGCTTCCAGAGACTACCGAAATATTATTGAGAG GTTACAGGACATCGTGTCTGCCCTGGAGGACCGACTCAGGCCCCTGGTCCAGGCCGAGCTGTCTGTGCTC GTGGATGTTCTACACAGACCGGAACTGCTTTTCCCTGAGAACACGGATGCCAGGAGGAAATGCGAAAGTG GAGGTTTCATCTGCAAGCTCATAAAACACACCAAGCAGCTGCTAGAGGAGAATGAAGAGAAACTGTGCAT TAAAGTCTTACAGACCCTCAGGGAAATGATGACCAAAGATAGAGGCTATGGAGAAAAGCAAATTTCCATT GATGAATTGGAAAATGCCGAGCTGCCACAGCCACCGGAAGCTGAGAACTCCACAGAGGAGCTTGAACCAA GTCCACCCCTGAGGCAACTGGAAGACCATAAAAGGGGTGAGGCACTCCGGCAAATTTTGGTCAACCGTTA CTATGGAAACATCAGACCTTCAGGAAGAAGAGAGAGCCTTACCAGCTTTGGCAATGGTCCACTATCACCA GGAGGACCCAGCAAGCCTGGGGGAGGAGGGGGAGGTCCTGGGTCCGGCTCCACAAGCCGGGGTGAGATGA GCCTGGCTGAGGTTCAATGTCATCTCGACAAGGAAGGCGCCTCCAATCTGGTCATCGACCTCATAATGAA TGCATCCAGCGACCGAGTATTCCACGAAAGCATTCTCCTGGCCATCGCACTTCTGGAAGGAGGCAACACC ACTATCCAGCACTCATTTTTCTGCCGGCTGACGGAAGATAAGAAATCAGAGAAGTTCTTCAAGGTCTTTT ACGATCGAATGAAGGTGGCCCAGCAGGAAATCAAGGCGACAGTGACCGTGAACACCAGCGATTTGGGAAA CAAAAAGAAAGATGATGAAGTGGACCGGGATGCCCCATCCCGGAAGAAAGCCAAAGAGCCCACAACACAG ATAACAGAAGAGGTCCGGGATCAGCTCTTGGAAGCATCTGCTGCCACCAGAAAAGCCTTTACCACCTTCC GGAGGGAGGCCGACCCTGATGACCATTACCAATCTGGGGAGGGCACCCAGGCTACAACGGACAAAGCCAA GGATGACCTAGAGATGAGTGCAGTCATCACCATCATGCAGCCTATCCTGCGCTTCCTGCAGCTCCTGTGT GAAAACCACAACCGCGATCTGCAGAATTTCCTTCGTTGCCAAAATAATAAGACCAACTACAACTTGGTGT GTGAGACACTGCAGTTTCTGGACTGTATCTGTGGGAGCACAACTGGAGGCCTTGGTCTTCTCGGACTGTA CATAAATGAAAAGAATGTAGCTCTTATCAACCAAACCCTGGAGAGTCTGACTGAGTACTGTCAAGGGCCT TGCCATGAAAACCAGAACTGCATCGCCACCCACGAGTCCAATGGCATCGATATCATCACAGCCCTCATCC TTAACGATATCAACCCTCTGGGGAAGAAACGGATGGACCTGGTGTTAGAACTGAAGAACAATGCTTCAAA GCTACTCCTGGCCATCATGGAAAGCAGACACGATAGTGAAAATGCAGAGAGGATCCTGTACAACATGAGG CCCAAGGAGCTGGTGGAAGTGATCAAGAAGGCCTACATGCAAGGCGAAGTGGAGTTTGAGGATGGGGAGA ACGGTGAGGATGGAGCAGCCTCCCCCAGGAACGTGGGCCATAACATCTACATCCTCGCTCACCAGTTGGC TCGGCATAACAAAGAACTTCAAACCATGCTGAAACCTGGAGGCCAGGTGGATGGCGATGAAGCTCTGGAG TTCTATGCGAAGCACACAGCACAAATTGAGATTGTCAGACTGGACCGAACTATGGAACAGATTGTCTTCC CTGTGCCCAGCATCTGTGAATTTCTGACTAAGGAATCGAAACTTCGAATATATTACACCACAGAGAGGGA TGAGCAAGGCAGCAAGATCAATGACTTCTTCCTGCGCTCCGAGGACCTCTTTAATGAGATGAACTGGCAG AAGAAACTTCGAGCCCAGCCTGTCTTGTACTGGTGCGCCCGAAACATGTCTTTCTGGAGCAGCATCTCCT TCAACCTGGCCGTCCTGATGAACCTGCTGGTGGCGTTTTTCTACCCATTTAAAGGAGTGAGAGGAGGAAC ACTAGAGCCGCACTGGTCCGGCCTCCTGTGGACAGCCATGCTCATCTCTCTGGCAATTGTCATTGCGCTG CCCAAGCCCCATGGCATCCGGGCCTTAATTGCTTCTACAATCCTACGACTGATATTTTCAGTTGGATTAC AGCCCACACTGTTTCTGCTGGGAGCTTTCAATGTCTGCAATAAAATCATCTTCCTGATGAGCTTTGTGGG CAACTGTGGGACCTTCACCAGAGGCTACCGAGCCATGGTTCTGGACGTTGAGTTCCTCTATCATTTGCTG TATCTACTGATCTGTGCCATGGGCCTCTTCGTACATGAGTTTTTCTATAGTTTGCTGCTTTTTGATTTAG TGTACAGAGAGGAGACTTTGCTTAATGTCATTAAAAGTGTCACCCGCAATGGACGGTCCATCATCTTGAC AGCGGTCCTGGCTCTGATTCTGGTTTACCTGTTCTCAATTGTGGGCTACCTGTTCTTCAAGGATGACTTT ATCTTGGAAGTAGACAGGTTGCCCAATGAGACAGCTGGTCCAGAAACTGGTGAGAGTTTGGCCAACGATT TCCTGTACTCTGATGTGTGCCGGGTAGAGACGGGGGAGAACTGCACCTCTCCGGCACCCAAAGAAGAGCT GCTCCCTGTGGAAGAAACGGAACAGGATAAGGAGCACACGTGTGAGACACTGCTCATGTGCATCGTCACC GTTCTGAGTCACGGGCTGCGGAGTGGGGGCGGAGTAGGAGATGTGCTCAGGAAGCCATCCAAAGAGGAGC CTCTGTTTGCTGCAAGGGTGATCTATGACCTCCTCTTCTTCTTCATGGTCATCATCATTGTCCTGAACCT CATTTTTGGGGTCATCATCGACACCTTTGCTGACCTGAGGAGTGAGAAGCAGAAGAAGGAAGAGATCTTA AAAACCACGTGCTTCATTTGTGGCTTGGAAAGGGACAAGTTTGACAATAAGACCGTCACCTTTGAAGAGC ACATTAAGGAAGAACACAACATGTGGCACTATCTGTGCTTCATCGTGCTGGTGAAAGTGAAGGACTCCAC CGAGTACACCGGGCCCGAGAGCTACGTGGCAGAGATGATCAGGGAAAGAAACCTTGATTGGTTCCCCAGG ATGAGAGCCATGTCCCTGGTCAGCAGTGACTCTGAAGGGGAACAGAACGAGCTGAGGAACCTGCAGGAGA AGCTGGAGTCCACCATGAAGCTGGTCACCAATCTTTCTGGCCAGCTGTCAGAACTAAAGGACCAGATGAC AGAACAAAGGAAGCAGAAACAAAGAATTGGCCTTCTAGGACATCCTCCTCACATGAATGTCAACCCACAG CAGCCAGCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001270596
Insert Size	8202 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001270596.1, NP_001257525.1
RefSeq Size	9739 bp
RefSeq ORF	8202 bp
Locus ID	25262
Gene Summary	This gene belongs to the Insp3 receptor family. It encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2012] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.

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