Yif1b (NM_198734) Rat Tagged ORF Clone

CAT#: RR200127

  • TrueORF®

Yif1b (Myc-DDK-tagged ORF) - Rat Yip1 interacting factor homolog B (S. cerevisiae) (Yif1b), transcript variant 1, (10 ug)



  "NM_198734" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Yip1b
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>NM_198734 ORF sequence, RR200127 may differ due to SNPs.
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCACGCGACAGGTTTGGCGGCGCCGGCGGGGACGCCCCGGCTGCGTAAGTGGCCCTCAAAGCGGAGG
GTCCCAGTGTCCCAACCGGGCATGGCTGATCCCCACCAGTTTTTTGACGACACGAGTTCAGCCCCGAGC
CGGGGCTATGGAGGCCAGCCATCACCTGGTAGCCTGGGTTACCCCACCTCTTCATCGGAGGCAGCCTTC
CTGGCTGCTCCTATGTCCAACATGGCGATGGCCTACGGGAGCAGCCTGGCTGCTCAGGGCAAAGAGCTG
GTAGATAAGAATATCGACCGCTTCATCCCTGTCAGCAAGCTCAAGTATTACTTCGCCGTGGACACCGTG
TACGTGGGCAAGAAGCTCGGACTGCTCGTCTTTCCCTACCTTCACCAGGACTGGGAGGTCCAGTACCAA
CAGGACACCCCCGTGGCGCCCCGCTTTGACATCAATGCTCCAGACCTCTACATTCCAGCCATGGCTTTC
ATAACCTACATCTTGGTGGCCGGCCTTGCACTGGGGACCCAGGACAGGTTCTCCCCAGACCTCCTGGGA
CTACAGGCGAGCTCGGCACTGGCTTGGCTGACTCTGGAAGTCGTAGCCATTCTGCTCAGTCTTTACCTG
GTCACTGTCAACACCGACCTTACCACCATTGACCTGGTGGCCTTCTTGGGTTACAAATATGTGGGGATG
ATTGGCGGGGTCCTCACAGGCCTGCTCTTTGGGAAGATTGGCTACTACCTGGTGCTTGCCTGGTGCTGT
GTGTCCATCTTTGTGTTCATGATCCGGACACTGCGGCTCAAGATCCTGGCCCAGGCAGCGGCCGAAGGA
GTGCCGGTGCGCGGGGCTCGGAACCAGCTGCGCATGTACTTGACTATGGCGGTGGCGGCTGCGCAGCCG
GTACTCATGTACTGGCTCACCTTCCACCTTGTACGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
>Peptide sequence encoded by RR200127
Blue=ORF Red=Cloning site Green=Tag(s)

MHATGLAAPAGTPRLRKWPSKRRVPVSQPGMADPHQFFDDTSSAPSRGYGGQPSPGSLGYPTSSSEAAF
LAAPMSNMAMAYGSSLAAQGKELVDKNIDRFIPVSKLKYYFAVDTVYVGKKLGLLVFPYLHQDWEVQYQ
QDTPVAPRFDINAPDLYIPAMAFITYILVAGLALGTQDRFSPDLLGLQASSALAWLTLEVVAILLSLYL
VTVNTDLTTIDLVAFLGYKYVGMIGGVLTGLLFGKIGYYLVLAWCCVSIFVFMIRTLRLKILAQAAAEG
VPVRGARNQLRMYLTMAVAAAQPVLMYWLTFHLVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_198734
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_198734.3
RefSeq Size 1183 bp
RefSeq ORF 936 bp
Locus ID 292768
UniProt ID Q6PEC3
MW 34 kDa
Gene Summary Involved in the anterograde traffic pathway from the endoplasmic reticulum to the plasma membrane and the organization of the Golgi architecture (Probable). Plays a key role in targeting to neuronal dendrites receptors such as HTR1A (PubMed:18685031).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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