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Usp4 (NM_001106093) Rat Tagged ORF Clone
CAT＃: RR200137
- TrueORF®
Usp4 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 2, (10 ug)
"NM_001106093" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200137 representing NM_001106093
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGGCCGGGGCACCCATGAGCGACCGGATGTGGAGACCCAGAAGACGGAGCTCGGAGCCTTGA TGGGGACCACGCTCCAGCGTGGGGCTCAGTGGTATCTGATCGACAGCCGGTGGTTCAAGCAGTGGAAGAA GTACGTCGGCTTTGACAGCTGGGACATGTACAACGTGGGGGAGCACAATCTCTTTCCTGGACCGATTGAC AACTCTGGGCTCTTCTCAGATCCTGAGAGTCAGACCTTGAAGGAACACTTAATCGATGAGCTGGACTATG TGTTGGTTCCAACTGAAGCCTGGAATAAATTGCTAAATTGGTATGGCTGTGTGGAAGGCCAGCAGCCTAT TGTCAGAAAAGTTGTGGAGCATGGCCTGTTTGTCAAGCACTGCAAAGTGGAAGTGTATTTGCTGGAGTTG AAGCTCTGTGAGAACAGTGACCCCACCAATGTGCTAAGTTGCCATTTTAGCAAAGCAGATACCATTGCAA CTATTGAGAAGGAGATGAGGAAGCTCTTCAACATCCCTGCAGAGCGTGAAACACGACTTTGGAACAAATA CATGAGCAACACCTATGAGCAGTTGAGCAAGCTAGACAACACTATCCAGGATGCTGGGCTGTACCAGGGT CAGGTGCTAGTAATTGAGCCCCAAAATGAAGACGGCACATGGCCCAGGCAGACCCTGCAATCAAATGGAG CTGGCTTCTCTGCCTCGTATAATTGCCAGGAGCCCCCATCACCTCATATCCAGCCTGGCCTCTGTGGACT CGGAAACCTGGGAAACACCTGTTTTATGAATTCTGCTTTGCAGTGCCTGAGCAACACTGGTCCACTGACT GAGTACTTTCTCAAAGATGAGTATGAGGCCGAGATCAACAGAGACAACCCTCTGGGGATGAAAGGGGAGA TTGCAGAGGCCTATGCAGAGCTCATCAAGCAGATGTGGTCTGGAAGGGACACCCACGTAGCACCACGGAT GTTCAAAACGCAAGTGGGACGTTTCGCCCCTCAGTTTTCTGGCTACCAACAACAAGACTCTCAAGAGCTG TTAGCCTTTATTCTAGATGGATTGCATGAAGACCTGAACCGAGTAAAGAAGAAGCCTTACCTGGAGCCAA AGGATGCCAATGGGCGACCAGATGCGGTGGTAGCAAAGGAAGCCTGGGAAAATCACAGGCTGAGGAATGA CTCTGTGATTGTGGATACTTTCCATGGCCTTTTCAAATCTACTTTGGTTTGCCCAGAATGTGCTAAAGTT TCTGTGACCTTTGACCCATTTTGCTATCTAACTCTCCCACTGCCTTTGAAGAAAGATCGAATTATGGAGG TCTTCCTGGTTCCTGCTGACCCTCATTGCAGACCTATCCAGTACCGTGTGACTGTGCCATTGATGGGGGC CATTTCTGACCTGTGTGAAGCACTCTCCAAGCTGTCTGGCATTGCTGCAGAAAATATGGTGGTCACTGAT GTGTATAATCACCGCTTCCACAAAATTTTCCAAATGGATGAAGGTTTAAGCCACATCACGCCTCGAGATG ACATTTTTGTGTATGAGATCTGCACCACTCCCATGGATGGCTCAGAATATATCACTCTCCCAGTCTACTT CAGAGAAAAGAAGTCCAGGCCATCGAGTACTTCCTCTGGGGCCGTGCTCTATGGACAGCCACTTCTTGTT TCTGTCCCTAAGCACAGACTAACCCTCGAGTCCTTATACCAGGCTGTTTGTGAACGTATCAGCCGCTATA TAAAGCAGCCTTTGCCTGAGGAGTTTCTCAGCTCTCCCTTAGAGCCTGGGGCCTGCAATGGCTCTAGGGG TAGCTATGAAGGAGATGAAGAAGAAATGGATCATCAAGAGGAAGGGAAAGAACAGCTTTCTGAAGTGGAA GAAAGTGGTGAGGACAGTCAGGGAGGGGACCCTACTGAGACCACCCAAAAGGCGAAAGGCCCGCCCCGCC ACAAGAGGCTTTTCACCTTCAGCCTCGTGAACTCCTGTGGAACTGCTGATATCAACTCGCTGGCCACTGA CGGAAAACTCCTCAAGCTCAACTCTCGATCCACACTGGCCATTGACTGGGACAGTGAGACCCGAAGCCTT TACTTTGATGAGCAAGAATCTGAGGCCTGTGAGAAGCACACGAGCATGTCCCAGCCTCAGAAGAAGAAGA AGGCTGCAATAGCCCTGCGGGAGTGCATCGAGCTCTTTACTACCATGGAGACCCTTGGGGAGCATGACCC CTGGTACTGTCCCACCTGTAAGAAGCACCAACAGGCAACAAAAAAGTTTGATTTGTGGTCTTTGCCCAAG ATCCTGGTGGTTCATCTCAAACGTTTCTCCTATAACAGATACTGGCGGGATAAACTGGACACAGTTGTGG AATTCCCAGTAAGAGCTCTGAACATGTCCGAGTTTGTGTGTGACCGGGCAGCAAGGCCTTATGTCTATGA CCTGATTGCTGTGTCCAATCACTATGGCGCTATGGGGGTCGGTCACTATACTGCATATGCGAAGAACAGA CTGAATGGGAAATGGTATTACTTTGATGATAGCAGCGTGTCCCTGGCCTCTGAGGATCAGATAGTGACGA AGGCCGCCTACGTGTTGTTTTACCAGCGTCGGGATGATGAGTGTCCCAGCACCTCCTCACCTGTCAGCTT CCCGGGTTCTGATGGAGGGGCGAAGCTCAGCAGCTCACAGCAGGACTTGGGGGAAGAGGAGGCTTACACC ATGGACACCAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200137 representing NM_001106093
Red=Cloning site Green=Tags(s) MAEGRGTHERPDVETQKTELGALMGTTLQRGAQWYLIDSRWFKQWKKYVGFDSWDMYNVGEHNLFPGPID NSGLFSDPESQTLKEHLIDELDYVLVPTEAWNKLLNWYGCVEGQQPIVRKVVEHGLFVKHCKVEVYLLEL KLCENSDPTNVLSCHFSKADTIATIEKEMRKLFNIPAERETRLWNKYMSNTYEQLSKLDNTIQDAGLYQG QVLVIEPQNEDGTWPRQTLQSNGAGFSASYNCQEPPSPHIQPGLCGLGNLGNTCFMNSALQCLSNTGPLT EYFLKDEYEAEINRDNPLGMKGEIAEAYAELIKQMWSGRDTHVAPRMFKTQVGRFAPQFSGYQQQDSQEL LAFILDGLHEDLNRVKKKPYLEPKDANGRPDAVVAKEAWENHRLRNDSVIVDTFHGLFKSTLVCPECAKV SVTFDPFCYLTLPLPLKKDRIMEVFLVPADPHCRPIQYRVTVPLMGAISDLCEALSKLSGIAAENMVVTD VYNHRFHKIFQMDEGLSHITPRDDIFVYEICTTPMDGSEYITLPVYFREKKSRPSSTSSGAVLYGQPLLV SVPKHRLTLESLYQAVCERISRYIKQPLPEEFLSSPLEPGACNGSRGSYEGDEEEMDHQEEGKEQLSEVE ESGEDSQGGDPTETTQKAKGPPRHKRLFTFSLVNSCGTADINSLATDGKLLKLNSRSTLAIDWDSETRSL YFDEQESEACEKHTSMSQPQKKKKAAIALRECIELFTTMETLGEHDPWYCPTCKKHQQATKKFDLWSLPK ILVVHLKRFSYNRYWRDKLDTVVEFPVRALNMSEFVCDRAARPYVYDLIAVSNHYGAMGVGHYTAYAKNR LNGKWYYFDDSSVSLASEDQIVTKAAYVLFYQRRDDECPSTSSPVSFPGSDGGAKLSSSQQDLGEEEAYT MDTN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001106093 |
| ORF Size | 2742 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001106093.2, NP_001099563.1 |
| RefSeq Size | 3955 bp |
| RefSeq ORF | 2745 bp |
| Locus ID | 290864 |
| MW | 103.7 kDa |
| Gene Summary | Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200137 | Usp4 (untagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 2, (10 ug) |
CNY 11,780.00 |
|
| RR200137L3 | Lenti ORF clone of Usp4 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 2, (10 ug) |
CNY 13,490.00 |
|
| RR200137L4 | Lenti ORF clone of Usp4 (mGFP-tagged ORF) - Rat ubiquitin specific peptidase 4 (proto-oncogene) (Usp4), transcript variant 2, (10 ug) |
CNY 14,820.00 |
