Shank2 (NM_201350) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CortBP1; ProSAP1; Spank-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200190 representing NM_201350 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGCAGCCCCACATCCAGTGAGGACGAGATGGCCCAGAGTTTCTCTGACTATTCTGTGGGCTCAG AGTCAGACAGCTCCAAAGAAGAGACCATCTATGACACGATCAGAGCCACCACAGAGAAGCCAGGCGGGGT GAAGATGGAGGACCTCCAGGGTAATACCCTGGTGATTCGAGTGGTCATCCAGGATCTGCAGCAGACGAAG TGCATTCGATTTAACCCGGACGCCACCGTGTGGGTGGCCAAGCAGCGGATACTGTGCACGCTGAACCAAG GCCTGAAGGATGTACTCAACTATGGGCTCTTTCAGCCCGCCAGCAACGGGCGTGACGGCAAGTTCCTGGA TGAGGAACGGCTCCTGCGGGAATACCCACAGCCCATGGGCCAGGGTGTGCCTTCCTTGGAGTTTCGCTAC AAGAAGCGTGTGTACAAGCAGTCCAACCTGGATGAGAAGCAGCTGGCCAGGCTCCACACAAAGACCAATC TGAAGAAGTTTATGGACCACACTCAGCATCGCTCAGTGGAGAAACTGGTCAAGCTCCTGGACAGAGGCCT GGATCCCAATTTCCATGACCTGGAGACTGGAGAGACGCCCCTGACCCTGGCTGCCCAGCTCGATGGCTCC ATGGAGGTCATCAAAGCTCTCAGAAATGGGGGTGCACATTTGGACTTCCGCTCCAGGGATGGGATGACTG CCCTTCACAAGGCTGCCCGGATGAGGAACCAGGTTGCCCTGAAGACCCTCCTGGAGCTTGGCGCCTCCCC AGATTACAAAGACAGCTACGGGCTCACACCGCTGTATCACACGGCCATTGTGGGGGGTGACCCTTACTGC TGCGAGCTCCTTCTCCACGAGCATGCCTCCGTGTGCTGCAAGGATGAAAATGGCTGGCACGAGATTCACC AGGCCTGCAGGTACGGCCATGTCCAGCATCTGGAACATCTGCTTTTCTATGGCGCAGACATGAGCGCACA GAATGCCTCAGGGAACACAGCCCTGCATATCTGCGCCCTCTACAATCAGGACAGCTGTGCCAGAGTCCTA CTGTTTCGGGGTGGAGACAAGGAGTTGAAAAACTACAACAGCCAGACACCGTTCCAGGTGGCTATAATAG CGGGGAACTTTGAACTTGCCGAGTACATCAAGAACCACAAGGAAACAGACATTGTGCCCTTCCGAGAGGC CCCAGCTTATTCCAACCGCAGGCGGCGGCCCCCCAACACATTGGCTGCCCCCAGAGTTCTTCTGCGTTCC AATAGCGACAACAACCTCAATGCCGGTGCGCCTGAATGGGCTGTCTGCTCTGCAGCCACCTCCCACCGAA GCCTCTCACCCCAGCTGCTGCAGCAGACACCCAGCAAACCTGATGGAGCCACAAAGAGCCTTGGAAGCTA CGCCCCTGGGCCCCGCAGCCGCTCTCCCTCACTCAACAGGCTGGGTGGCGCTGGTGAGGATGGCAAGAGG CCACAGCCGCCACATTGGCATGTGGGGTCGCCCTTCACTCCTGGTGCCAACAAGGACTCCCTCTCGACCT TTGAGTACCCAGGGCCCCGGAGGAAGCTGTATAGTGCGGTGCCTGGGAGACTCTTCGTCGCCATCAAGCC ATACCAACCCCAAGTCGACGGCGAGATCCCCCTTCACCGAGGCGACAGGGTCAAAGTTCTGAGCATCGGC GAGGGCGGCTTCTGGGAAGGCAGCGCCCGTGGCCACATCGGGTGGTTCCCAGCTGAGTGTGTGGAGGAGG TACAATGTAAGCCCCGGGACAGCCAAGCAGAAACCCGTGCGGACCGCAGCAAGAAGCTCTTTCGGCATTA CACAGTGGGCTCCTATGACAGCTTTGATGCTGCCAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTG CAGAAGAAAGACAACGAGGGCTTTGGATTTGTGCTCCGAGGGGCAAAAGCTGATACCCCCATTGAGGAAT TCACACCCACGCCGGCATTTCCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGAGGGGTGGCATGGCA AGCCGGACTAAGGACCGGGGACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGG CAGGTGGTGAACATGATCCGCCAGGGAGGGAATCACCTCGTCCTTAAGGTCGTCACGGTGACCAGGAATC TAGACCCTGATGATACAGCCAGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCAC CCTGCGTTCCAAGTCCATGACAGCGGAATTGGAGGAACTCGATAAACCGGAAGAGATAGTCCCAGCCTCC AAGCCCTCCAGGACTGCAGAGAACGTGGCCATAGAATCCAGGGTGGCAACCATCAAGCAGCGGCCCACAA GCCGGTGCTTTCCAGCTGCCTCTGATGTGAACTCCGTGTACGAGCGCCAAGGGATTGCTGTAATGACGCC CACGGTCCCTGGGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCGAAGGCAGAAATCG ATAGACAGCAGAATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCCCCAATGCTGA AGTTCACCCGAAGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGTCTGTGCCCCC CTCTCCCCCTCCACCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGTCTATGGGACA ATTAAGCCTGCGTTCAACCAGAACCCCGTCGCCAAGGTGCCCCCAGCCACCAGGTCTGACACTGTGGCCA CCATGATGCGGGAAAAGGGGATGTTCTACAGGAGAGAGCTGGACCGCTTTTCCCTGGACTCAGAAGACGT CTACAGCCGCAGCCCCGCCCCACAGGCTGCCTTCCGCACCAAGCGGGGACAGATGCCTGAGAACCCGTAC TCGGAGGTGGGAAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGGCGGAAGGGCATGC TGGTAAAGCAGTCCAACGTGGAGGACAGCCCTGAGAAGACGTGCTCCATCCCCATCCCAACCATCATAGT CAAGGAACCCTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGATCGACCCCCAGGCC ACTGAGCCCGGCCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCCTTCGCGGCAGCCATCGCTG GGGCCGTGCGTGACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCGGCCTTCCTCTCCACCGACCT GGGAGATGAGGACGTGGGTCTGGGGCCTCCTGCTCCCCGGATGCAGCCCTCCAAGTTTCCCGAGGAGGGT GGGTTTGGTGATGAGGATGAAACGGAACAGCCGCTATTGCCTACCCCTGGAGCAGCGCCCAGGGAGCTGG AGAACCACTTCCTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCCTGAGTTCCACATC CAAAGCCAAGGGGCCTGAAAGTGGCCCAGCAGCCGCCCTCAAGAGCAGCAGCCCAGCCAGCCCTGAGAAT TACGTGCACCCACTCACGGGGCGGCTGCTCGACCCCAGCTCCCCGCTGGCCCTGGCACTGTCCGCCAGGG ACCGAGCCATGCAGGAGTCCCAGCAAGGACACAAGGGGGAGGCCCCCAAGGCCGACCTTAACAAGCCTCT CTACATCGATACCAAAATGCGGCCCAGTGTGGAGTCCGGCTTTCCACCGGTCACCAGACAGAACACCAGG GGTCCCCTGCGAAGGCAGGAGACAGAGAACAAGTATGAGACGGACCTGAGCAAGGACCGGAGGGCTGACG ACAAGAAGAACATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCCGGCCTACTGATGGTGCA CACAGTGGACATTCCCGTGGCGGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGATGGGGATACAAAG CCAGACCACTCACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTACAGATCTCCGCTG CCCCGGAGCCCGCCGCTGCGCCCGGCAGGACCATCGTGGCAGCGGGCTCCGTGGAAGAGGCGGTAATTCT GCCATTCCGCATCCCCCCTCCCCCTCTGGCGTCCGTGGACTTGGATGAGGACTTTCTTTTCACAGAACCA TTGCCTCCTCCCCTGGAATTCGCCAATAGTTTTGATATCCCCGACGACCGGGCAGCTTCAGTTCCCGCTC TGGCTGACCTGGTCAAGCAGAAGAAAAGTGACACCCCTCAGCCCCCTTCGTTGAACTCCAGCCAACCAGC CAACTCTACAGACAGTAAGAAGCCAGCCGGCATCTCAAACTGTCTGCCCTCCTCATTCCTGCCACCCCCC GAAAGCTTTGACGCGGTCACCGACTCGGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACC TGGAGACAACCAGCACCATCTCCACAGTGTCCAGCATCTCCACGCTGTCTTCAGAGGGCGGGGAGAGCAT GGATACTTGCACAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGTACCTCCAAAGCCA AAAATGAAGCCCATCGTTCACAAAAGCAACGCACTTTACCAAGACACGCTCCCAGAAGAGGACACAGATG GCTTTGTGATCCCCCCACCTGCACCCCCGCCCCCGCCCGGCAGTGCCCAGGCCGGTGTGGCGAAGGTCAT CCAGCCAAGGACCTCCAAGTTGTGGGGTGATGTTACAGAGGTCAAAAGCCCAATTCTCTCAGGCCCAAAG GCAAATGTCATTAGTGAGTTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCGGTCAAGCCTGGGG AAGGGCTGGAGCTGCCCGTGGGAGCCAAGTCAGCTAACCTCGCTCCAAGAAGCCCGGAGGTCATGAGCAC TGTCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTCCGCCCCGGAACCTCCCAGCCCATCACCCTA CAGAGCCGGCCCCCTGACTATGAAAGCAGGACCTCAGGACCGAGACGTGCCCCAAGCCCTGTGGTTTCGC CAACGGAATTGAGCAAAGAGATTCTGCCCACCCCTCCGTCTGCTGCAGCAGCCTCTCCCTCCCCCACACT CTCAGATGTCTTTAGCCTTCCAAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAACCCAGCAGGACGC AGCAGGTCACCATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCTTTTACAACTAAGCCTGTCC ACCTGTGGACGAAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGGGTGAACACAAGGAGACGTT CATGGACAATGAGATTGACGGCAGCCATCTGCCAAACCTCCAGAAGGAAGACTTGATAGATCTTGGGGTG ACTCGAGTTGGGCACAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200190 representing NM_201350 Red=Cloning site Green=Tags(s) MPRSPTSSEDEMAQSFSDYSVGSESDSSKEETIYDTIRATTEKPGGVKMEDLQGNTLVIRVVIQDLQQTK CIRFNPDATVWVAKQRILCTLNQGLKDVLNYGLFQPASNGRDGKFLDEERLLREYPQPMGQGVPSLEFRY KKRVYKQSNLDEKQLARLHTKTNLKKFMDHTQHRSVEKLVKLLDRGLDPNFHDLETGETPLTLAAQLDGS MEVIKALRNGGAHLDFRSRDGMTALHKAARMRNQVALKTLLELGASPDYKDSYGLTPLYHTAIVGGDPYC CELLLHEHASVCCKDENGWHEIHQACRYGHVQHLEHLLFYGADMSAQNASGNTALHICALYNQDSCARVL LFRGGDKELKNYNSQTPFQVAIIAGNFELAEYIKNHKETDIVPFREAPAYSNRRRRPPNTLAAPRVLLRS NSDNNLNAGAPEWAVCSAATSHRSLSPQLLQQTPSKPDGATKSLGSYAPGPRSRSPSLNRLGGAGEDGKR PQPPHWHVGSPFTPGANKDSLSTFEYPGPRRKLYSAVPGRLFVAIKPYQPQVDGEIPLHRGDRVKVLSIG EGGFWEGSARGHIGWFPAECVEEVQCKPRDSQAETRADRSKKLFRHYTVGSYDSFDAASDCIIEDKTVVL QKKDNEGFGFVLRGAKADTPIEEFTPTPAFPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHR QVVNMIRQGGNHLVLKVVTVTRNLDPDDTARKKAPPPPKRAPTTALTLRSKSMTAELEELDKPEEIVPAS KPSRTAENVAIESRVATIKQRPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKS IDSRIFLSGITEEERQFLAPPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGT IKPAFNQNPVAKVPPATRSDTVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPY SEVGKIASKAVYVPAKPARRKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQA TEPGQLRPDDSLTVSSPFAAAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEG GFGDEDETEQPLLPTPGAAPRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPEN YVHPLTGRLLDPSSPLALALSARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTR GPLRRQETENKYETDLSKDRRADDKKNMLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTK PDHSPSTVPEGVPKTEGALQISAAPEPAAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEP LPPPLEFANSFDIPDDRAASVPALADLVKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPP ESFDAVTDSGIEEVDSRSSSDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKP KMKPIVHKSNALYQDTLPEEDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPK ANVISELNSILQQMNRGKSVKPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITL QSRPPDYESRTSGPRRAPSPVVSPTELSKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGR SRSPSPSILQQPISNKPFTTKPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGV TRVGHRMNIERALKQLLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_201350
ORF Size	5517 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_201350.1, NP_958738.1
RefSeq Size	5625 bp
RefSeq ORF	5520 bp
Locus ID	171093
UniProt ID	Q9QX74
MW	199.9 kDa
Gene Summary	binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

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