Shank2 (NM_133440) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CortBP1; ProSAP1; Spank-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200191 representing NM_133440 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCGTCCCCGGCGGTGGAGCAGCCACCGTGATGATGACCGGTTACAATAATGGTCGCTATCCCC GGAATTCTCTCTACAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAGAAGAAAGACAACGAGGG CTTTGGATTTGTGCTCCGAGGGGCAAAAGCTGATACCCCCATTGAGGAATTCACACCCACGCCGGCATTT CCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGAGGGGTGGCATGGCAAGCCGGACTAAGGACCGGGG ACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGGCAGGTGGTGAACATGATCCG CCAGGGAGGGAATCACCTCGTCCTTAAGGTCGTCACGGTGACCAGGAATCTAGACCCTGATGATACAGCC AGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCACCCTGCGTTCCAAGTCCATGA CAGCGGAATTGGAGGAACTCGTGGATAAAGCCTCAGTCCGGAAGAAGAAGGATAAACCGGAAGAGATAGT CCCAGCCTCCAAGCCCTCCAGGACTGCAGAGAACGTGGCCATAGAATCCAGGGTGGCAACCATCAAGCAG CGGCCCACAAGCCGGTGCTTTCCAGCTGCCTCTGATGTGAACTCCGTGTACGAGCGCCAAGGGATTGCTG TAATGACGCCCACGGTCCCTGGGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCGAAG GCAGAAATCGATAGACAGCAGAATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCC CCAATGCTGAAGTTCACCCGAAGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGT CTGTGCCCCCCTCTCCCCCTCCACCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGT CTATGGGACAATTAAGCCTGCGTTCAACCAGAACCCCGTCGCCAAGGTGCCCCCAGCCACCAGGTCTGAC ACTGTGGCCACCATGATGCGGGAAAAGGGGATGTTCTACAGGAGAGAGCTGGACCGCTTTTCCCTGGACT CAGAAGACGTCTACAGCCGCAGCCCCGCCCCACAGGCTGCCTTCCGCACCAAGCGGGGACAGATGCCTGA GAACCCGTACTCGGAGGTGGGAAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGGCGG AAGGGCATGCTGGTAAAGCAGTCCAACGTGGAGGACAGCCCTGAGAAGACGTGCTCCATCCCCATCCCAA CCATCATAGTCAAGGAACCCTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGATCGA CCCCCAGGCCACTGAGCCCGGCCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCCTTCGCGGCA GCCATCGCTGGGGCCGTGCGTGACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCGGCCTTCCTCT CCACCGACCTGGGAGATGAGGACGTGGGTCTGGGGCCTCCTGCTCCCCGGATGCAGCCCTCCAAGTTTCC CGAGGAGGGTGGGTTTGGTGATGAGGATGAAACGGAACAGCCGCTATTGCCTACCCCTGGAGCAGCGCCC AGGGAGCTGGAGAACCACTTCCTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCCTGA GTTCCACATCCAAAGCCAAGGGGCCTGAAAGTGGCCCAGCAGCCGCCCTCAAGAGCAGCAGCCCAGCCAG CCCTGAGAATTACGTGCACCCACTCACGGGGCGGCTGCTCGACCCCAGCTCCCCGCTGGCCCTGGCACTG TCCGCCAGGGACCGAGCCATGCAGGAGTCCCAGCAAGGACACAAGGGGGAGGCCCCCAAGGCCGACCTTA ACAAGCCTCTCTACATCGATACCAAAATGCGGCCCAGTGTGGAGTCCGGCTTTCCACCGGTCACCAGACA GAACACCAGGGGTCCCCTGCGAAGGCAGGAGACAGAGAACAAGTATGAGACGGACCTGAGCAAGGACCGG AGGGCTGACGACAAGAAGAACATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCCGGCCTAC TGATGGTGCACACAGTGGACATTCCCGTGGCGGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGATGG GGATACAAAGCCAGACCACTCACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTACAG ATCTCCGCTGCCCCGGAGCCCGCCGCTGCGCCCGGCAGGACCATCGTGGCAGCGGGCTCCGTGGAAGAGG CGGTAATTCTGCCATTCCGCATCCCCCCTCCCCCTCTGGCGTCCGTGGACTTGGATGAGGACTTTCTTTT CACAGAACCATTGCCTCCTCCCCTGGAATTCGCCAATAGTTTTGATATCCCCGACGACCGGGCAGCTTCA GTTCCCGCTCTGGCTGACCTGGTCAAGCAGAAGAAAAGTGACACCCCTCAGCCCCCTTCGTTGAACTCCA GCCAACCAGCCAACTCTACAGACAGTAAGAAGCCAGCCGGCATCTCAAACTGTCTGCCCTCCTCATTCCT GCCACCCCCCGAAAGCTTTGACGCGGTCACCGACTCGGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGC GACCACCACCTGGAGACAACCAGCACCATCTCCACAGTGTCCAGCATCTCCACGCTGTCTTCAGAGGGCG GGGAGAGCATGGATACTTGCACAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGTACC TCCAAAGCCAAAAATGAAGCCCATCGTTCACAAAAGCAACGCACTTTACCAAGACACGCTCCCAGAAGAG GACACAGATGGCTTTGTGATCCCCCCACCTGCACCCCCGCCCCCGCCCGGCAGTGCCCAGGCCGGTGTGG CGAAGGTCATCCAGCCAAGGACCTCCAAGTTGTGGGGTGATGTTACAGAGGTCAAAAGCCCAATTCTCTC AGGCCCAAAGGCAAATGTCATTAGTGAGTTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCGGTC AAGCCTGGGGAAGGGCTGGAGCTGCCCGTGGGAGCCAAGTCAGCTAACCTCGCTCCAAGAAGCCCGGAGG TCATGAGCACTGTCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTCCGCCCCGGAACCTCCCAGCC CATCACCCTACAGAGCCGGCCCCCTGACTATGAAAGCAGGACCTCAGGACCGAGACGTGCCCCAAGCCCT GTGGTTTCGCCAACGGAATTGAGCAAAGAGATTCTGCCCACCCCTCCGTCTGCTGCAGCAGCCTCTCCCT CCCCCACACTCTCAGATGTCTTTAGCCTTCCAAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAACCC AGCAGGACGCAGCAGGTCACCATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCTTTTACAACT AAGCCTGTCCACCTGTGGACGAAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGGGTGAACACA AGGAGACGTTCATGGACAATGAGATTGACGGCAGCCATCTGCCAAACCTCCAGAAGGAAGACTTGATAGA TCTTGGGGTGACTCGAGTTGGGCACAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200191 representing NM_133440 Red=Cloning site Green=Tags(s) MMSVPGGGAATVMMTGYNNGRYPRNSLYSDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAF PALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTA RKKAPPPPKRAPTTALTLRSKSMTAELEELVDKASVRKKKDKPEEIVPASKPSRTAENVAIESRVATIKQ RPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAP PMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRSD TVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARR KGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAA AIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAAP RELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALAL SARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKDR RADDKKNMLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQ ISAAPEPAAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAAS VPALADLVKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSS DHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEE DTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKSV KPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSP VVSPTELSKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTT KPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133440
ORF Size	3777 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133440.1, NP_597684.1
RefSeq Size	7688 bp
RefSeq ORF	3780 bp
Locus ID	171093
UniProt ID	Q9QX74
MW	135.1 kDa
Gene Summary	binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

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