Shank2 (NM_133441) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CortBP1; ProSAP1; Spank-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200192 representing NM_133441 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCTTTGTTAAATGCCTTCACCAAGAAGGAAGTGCCCTTCCGAGAGGCCCCAGCTTATTCCAACC GCAGGCGGCGGCCCCCCAACACATTGGCTGCCCCCAGAGTTCTTCTGCGTTCCAATAGCGACAACAACCT CAATGCCGGTGCGCCTGAATGGGCTGTCTGCTCTGCAGCCACCTCCCACCGAAGCCTCTCACCCCAGCTG CTGCAGCAGACACCCAGCAAACCTGATGGAGCCACAAAGAGCCTTGGAAGCTACGCCCCTGGGCCCCGCA GCCGCTCTCCCTCACTCAACAGGCTGGGTGGCGCTGGTGAGGATGGCAAGAGGCCACAGCCGCCACATTG GCATGTGGGGTCGCCCTTCACTCCTGGTGCCAACAAGGACTCCCTCTCGACCTTTGAGTACCCAGGGCCC CGGAGGAAGCTGTATAGTGCGGTGCCTGGGAGACTCTTCGTCGCCATCAAGCCATACCAACCCCAAGTCG ACGGCGAGATCCCCCTTCACCGAGGCGACAGGGTCAAAGTTCTGAGCATCGGCGAGGGCGGCTTCTGGGA AGGCAGCGCCCGTGGCCACATCGGGTGGTTCCCAGCTGAGTGTGTGGAGGAGGTACAATGTAAGCCCCGG GACAGCCAAGCAGAAACCCGTGCGGACCGCAGCAAGAAGCTCTTTCGGCATTACACAGTGGGCTCCTATG ACAGCTTTGATGCTGCCAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAGAAGAAAGACAACGA GGGCTTTGGATTTGTGCTCCGAGGGGCAAAAGCTGATACCCCCATTGAGGAATTCACACCCACGCCGGCA TTTCCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGAGGGGTGGCATGGCAAGCCGGACTAAGGACCG GGGACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGGCAGGTGGTGAACATGAT CCGCCAGGGAGGGAATCACCTCGTCCTTAAGGTCGTCACGGTGACCAGGAATCTAGACCCTGATGATACA GCCAGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCACCCTGCGTTCCAAGTCCA TGACAGCGGAATTGGAGGAACTCGTGGATAAAGCCTCAGTCCGGAAGAAGAAGGATAAACCGGAAGAGAT AGTCCCAGCCTCCAAGCCCTCCAGGACTGCAGAGAACGTGGCCATAGAATCCAGGGTGGCAACCATCAAG CAGCGGCCCACAAGCCGGTGCTTTCCAGCTGCCTCTGATGTGAACTCCGTGTACGAGCGCCAAGGGATTG CTGTAATGACGCCCACGGTCCCTGGGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCG AAGGCAGAAATCGATAGACAGCAGAATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCT CCCCCAATGCTGAAGTTCACCCGAAGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCAC AGTCTGTGCCCCCCTCTCCCCCTCCACCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAG AGTCTATGGGACAATTAAGCCTGCGTTCAACCAGAACCCCGTCGCCAAGGTGCCCCCAGCCACCAGGTCT GACACTGTGGCCACCATGATGCGGGAAAAGGGGATGTTCTACAGGAGAGAGCTGGACCGCTTTTCCCTGG ACTCAGAAGACGTCTACAGCCGCAGCCCCGCCCCACAGGCTGCCTTCCGCACCAAGCGGGGACAGATGCC TGAGAACCCGTACTCGGAGGTGGGAAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGG CGGAAGGGCATGCTGGTAAAGCAGTCCAACGTGGAGGACAGCCCTGAGAAGACGTGCTCCATCCCCATCC CAACCATCATAGTCAAGGAACCCTCCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGAT CGACCCCCAGGCCACTGAGCCCGGCCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCCTTCGCG GCAGCCATCGCTGGGGCCGTGCGTGACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCGGCCTTCC TCTCCACCGACCTGGGAGATGAGGACGTGGGTCTGGGGCCTCCTGCTCCCCGGATGCAGCCCTCCAAGTT TCCCGAGGAGGGTGGGTTTGGTGATGAGGATGAAACGGAACAGCCGCTATTGCCTACCCCTGGAGCAGCG CCCAGGGAGCTGGAGAACCACTTCCTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCC TGAGTTCCACATCCAAAGCCAAGGGGCCTGAAAGTGGCCCAGCAGCCGCCCTCAAGAGCAGCAGCCCAGC CAGCCCTGAGAATTACGTGCACCCACTCACGGGGCGGCTGCTCGACCCCAGCTCCCCGCTGGCCCTGGCA CTGTCCGCCAGGGACCGAGCCATGCAGGAGTCCCAGCAAGGACACAAGGGGGAGGCCCCCAAGGCCGACC TTAACAAGCCTCTCTACATCGATACCAAAATGCGGCCCAGTGTGGAGTCCGGCTTTCCACCGGTCACCAG ACAGAACACCAGGGGTCCCCTGCGAAGGCAGGAGACAGAGAACAAGTATGAGACGGACCTGAGCAAGGAC CGGAGGGCTGACGACAAGAAGAACATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCCGGCC TACTGATGGTGCACACAGTGGACATTCCCGTGGCGGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGA TGGGGATACAAAGCCAGACCACTCACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTA CAGATCTCCGCTGCCCCGGAGCCCGCCGCTGCGCCCGGCAGGACCATCGTGGCAGCGGGCTCCGTGGAAG AGGCGGTAATTCTGCCATTCCGCATCCCCCCTCCCCCTCTGGCGTCCGTGGACTTGGATGAGGACTTTCT TTTCACAGAACCATTGCCTCCTCCCCTGGAATTCGCCAATAGTTTTGATATCCCCGACGACCGGGCAGCT TCAGTTCCCGCTCTGGCTGACCTGGTCAAGCAGAAGAAAAGTGACACCCCTCAGCCCCCTTCGTTGAACT CCAGCCAACCAGCCAACTCTACAGACAGTAAGAAGCCAGCCGGCATCTCAAACTGTCTGCCCTCCTCATT CCTGCCACCCCCCGAAAGCTTTGACGCGGTCACCGACTCGGGGATCGAGGAGGTGGACAGCCGGAGTAGC AGCGACCACCACCTGGAGACAACCAGCACCATCTCCACAGTGTCCAGCATCTCCACGCTGTCTTCAGAGG GCGGGGAGAGCATGGATACTTGCACAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGT ACCTCCAAAGCCAAAAATGAAGCCCATCGTTCACAAAAGCAACGCACTTTACCAAGACACGCTCCCAGAA GAGGACACAGATGGCTTTGTGATCCCCCCACCTGCACCCCCGCCCCCGCCCGGCAGTGCCCAGGCCGGTG TGGCGAAGGTCATCCAGCCAAGGACCTCCAAGTTGTGGGGTGATGTTACAGAGGTCAAAAGCCCAATTCT CTCAGGCCCAAAGGCAAATGTCATTAGTGAGTTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCG GTCAAGCCTGGGGAAGGGCTGGAGCTGCCCGTGGGAGCCAAGTCAGCTAACCTCGCTCCAAGAAGCCCGG AGGTCATGAGCACTGTCTCAGGTACACGGAGCACGACGGTCACCTTCACTGTCCGCCCCGGAACCTCCCA GCCCATCACCCTACAGAGCCGGCCCCCTGACTATGAAAGCAGGACCTCAGGACCGAGACGTGCCCCAAGC CCTGTGGTTTCGCCAACGGAATTGAGCAAAGAGATTCTGCCCACCCCTCCGTCTGCTGCAGCAGCCTCTC CCTCCCCCACACTCTCAGATGTCTTTAGCCTTCCAAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAA CCCAGCAGGACGCAGCAGGTCACCATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCTTTTACA ACTAAGCCTGTCCACCTGTGGACGAAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGGGTGAAC ACAAGGAGACGTTCATGGACAATGAGATTGACGGCAGCCATCTGCCAAACCTCCAGAAGGAAGACTTGAT AGATCTTGGGGTGACTCGAGTTGGGCACAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200192 representing NM_133441 Red=Cloning site Green=Tags(s) MKSLLNAFTKKEVPFREAPAYSNRRRRPPNTLAAPRVLLRSNSDNNLNAGAPEWAVCSAATSHRSLSPQL LQQTPSKPDGATKSLGSYAPGPRSRSPSLNRLGGAGEDGKRPQPPHWHVGSPFTPGANKDSLSTFEYPGP RRKLYSAVPGRLFVAIKPYQPQVDGEIPLHRGDRVKVLSIGEGGFWEGSARGHIGWFPAECVEEVQCKPR DSQAETRADRSKKLFRHYTVGSYDSFDAASDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPA FPALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDT ARKKAPPPPKRAPTTALTLRSKSMTAELEELVDKASVRKKKDKPEEIVPASKPSRTAENVAIESRVATIK QRPTSRCFPAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLA PPMLKFTRSLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRS DTVATMMREKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPAR RKGMLVKQSNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFA AAIAGAVRDREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAA PRELENHFLGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALA LSARDRAMQESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKD RRADDKKNMLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGAL QISAAPEPAAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAA SVPALADLVKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSS SDHHLETTSTISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPE EDTDGFVIPPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKS VKPGEGLELPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPS PVVSPTELSKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFT TKPVHLWTKPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_133441
ORF Size	4410 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_133441.1, NP_597685.1
RefSeq Size	8182 bp
RefSeq ORF	4413 bp
Locus ID	171093
UniProt ID	Q9QX74
MW	158.3 kDa
Gene Summary	binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.