Shank2 (NM_001004133) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	CortBP1; ProSAP1; Spank-3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR200193 representing NM_001004133 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGAGCGTCCCCGGCGGTGGAGCAGCCACCGTGATGATGACCGGTTACAATAATGGTCGCTATCCCC GGAATTCTCTCTACAGTGACTGCATTATTGAGGACAAGACGGTGGTCCTGCAGAAGAAAGACAACGAGGG CTTTGGATTTGTGCTCCGAGGGGCAAAAGCTGATACCCCCATTGAGGAATTCACACCCACGCCGGCATTT CCAGCCCTGCAGTACCTGGAGTCCGTGGATGAAGGAGGGGTGGCATGGCAAGCCGGACTAAGGACCGGGG ACTTCTTGATTGAGGTTAACAATGAAAATGTCGTCAAGGTGGGCCACAGGCAGGTGGTGAACATGATCCG CCAGGGAGGGAATCACCTCGTCCTTAAGGTCGTCACGGTGACCAGGAATCTAGACCCTGATGATACAGCC AGAAAGAAAGCTCCCCCACCTCCAAAGCGGGCTCCGACCACGGCCCTCACCGTGCGTTCCAAGTCCATGA CAGCGGAATTGGAGGAACTCGTGGATAAAGATAAACCGGAAGAGATAGTCCCAGCCTCCAAGCCCTCCAG GACTGCAGAGAACGTGGCCATAGAATCCAGGGTGGCAACCATCAAGCAGCGGCCCACAAGCCGGTGCTTT CCAGCTGCCTCTGATGTGAACTCCGTGTACGAGCGCCAAGGGATTGCTGTAATGACGCCCACGGTCCCTG GGAGCCCGAAAGGCCCATTTCTGGGCCTCCCTCGAGGTACGATGCGAAGGCAGAAATCGATAGACAGCAG AATCTTTCTATCAGGGATAACAGAGGAAGAGCGGCAGTTTCTGGCTCCCCCAATGCTGAAGTTCACCCGA AGCTTGTCCATGCCAGACACTTCTGAGGACATCCCCCCTCCGCCACAGTCTGTGCCCCCCTCTCCCCCTC CACCTTCCCCCACCACATACAACTGTCCCAGGTCCCCGACTCCAAGAGTCTATGGGACAATTAAGCCTGC GTTCAACCAGAACCCCGTCGCCAAGGTGCCCCCAGCCACCAGGTCTGACACTGTGGCCACCATGATGCGG GAAAAGGGGATGTTCTACAGGAGAGAGCTGGACCGCTTTTCCCTGGACTCAGAAGACGTCTACAGCCGCA GCCCCGCCCCACAGGCTGCCTTCCGCACCAAGCGGGGACAGATGCCTGAGAACCCGTACTCGGAGGTGGG AAAGATAGCCAGCAAGGCCGTCTATGTCCCTGCCAAGCCAGCCAGGCGGAAGGGCATGCTGGTAAAGCAG TCCAACGTGGAGGACAGCCCTGAGAAGACGTGCTCCATCCCCATCCCAACCATCATAGTCAAGGAACCCT CCACCAGCAGCAGCGGCAAGAGCAGCCAGGGGAGCAGCATGGAGATCGACCCCCAGGCCACTGAGCCCGG CCAGCTGCGGCCAGATGACAGCCTCACCGTCAGCAGCCCTTTCGCGGCAGCCATCGCTGGGGCCGTGCGT GACCGGGAGAAGCGTCTGGAAGCCAGGAGGAATTCCCCGGCCTTCCTCTCCACCGACCTGGGAGATGAGG ACGTGGGTCTGGGGCCTCCTGCTCCCCGGATGCAGCCCTCCAAGTTTCCCGAGGAGGGTGGGTTTGGTGA TGAGGATGAAACGGAACAGCCGCTATTGCCTACCCCTGGAGCAGCGCCCAGGGAGCTGGAGAACCACTTC CTAGGTGGTGGTGAGGCTGGTGCTCAGGGGGAGGCTGGGGGACCCCTGAGTTCCACATCCAAAGCCAAGG GGCCTGAAAGTGGCCCAGCAGCCGCCCTCAAGAGCAGCAGCCCAGCCAGCCCTGAGAATTACGTGCACCC ACTCACGGGGCGGCTGCTCGACCCCAGCTCCCCGCTGGCCCTGGCACTGTCCGCCAGGGACCGAGCCATG CAGGAGTCCCAGCAAGGACACAAGGGGGAGGCCCCCAAGGCCGACCTTAACAAGCCTCTTTACATCGATA CCAAAATGCGGCCCAGTGTGGAGTCCGGCTTTCCACCGGTCACCAGACAGAACACCAGGGGTCCCCTGCG AAGGCAGGAGACAGAGAACAAGTATGAGACGGACCTGAGCAAGGACCGGAGGGCTGACGACAAGAAGAAC ATGCTGATCAACATCGTGGACACTGCCCAGCAGAAGTCAGCCGGCCTACTGATGGTGCACACAGTGGACA TTCCCGTGGCGGGGCCACCCCTGGAGGAAGAGGAGGACAGAGAGGATGGGGATACAAAGCCAGACCACTC ACCCTCCACAGTGCCAGAAGGCGTTCCCAAAACCGAAGGTGCTTTACAGATCTCCGCTGCCCCGGAGCCC GCCGCTGCGCCCGGCAGGACCATCGTGGCAGCGGGCTCCGTGGAAGAGGCGGTAATTCTGCCATTCCGCA TCCCCCCTCCCCCTCTGGCGTCCGTGGACTTGGATGAGGACTTTCTTTTCACAGAACCATTGCCTCCTCC CCTGGAATTCGCCAATAGTTTTGATATCCCCGACGACCGGGCAGCTTCAGTTCCCGCTCTGGCTGACCTG GTCAAGCAGAAGAAAAGTGACACCCCTCAGCCCCCTTCGTTGAACTCCAGCCAACCAGCCAACTCTACAG ACAGTAAGAAGCCAGCCGGCATCTCAAACTGTCTGCCCTCCTCATTCCTGCCACCCCCCGAAAGCTTTGA CGCGGTCACCGACTCGGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACCTGGAGACAACC AGCACCATCTCCACAGTGTCCAGCATCTCCACGCTGTCTTCAGAGGGCGGGGAGAGCATGGATACTTGCA CAGTCTATGCAGACGGGCAAGCCTTTGTGGTTGACAAGCCCCCAGTACCTCCAAAGCCAAAAATGAAGCC CATCGTTCACAAAAGCAACGCACTTTACCAAGACACGCTCCCAGAAGAGGACACAGATGGCTTTGTGATC CCCCCACCTGCACCCCCGCCCCCGCCCGGCAGTGCCCAGGCCGGTGTGGCGAAGGTCATCCAGCCAAGGA CCTCCAAGTTGTGGGGTGATGTTACAGAGGTCAAAAGCCCAATTCTCTCAGGCCCAAAGGCAAATGTCAT TAGTGAGTTAAACTCCATTCTGCAGCAGATGAACAGGGGGAAATCGGTCAAGCCTGGGGAAGGGCTGGAG CTGCCCGTGGGAGCCAAGTCAGCTAACCTCGCTCCAAGAAGCCCGGAGGTCATGAGCACTGTCTCAGGTA CACGGAGCACGACGGTCACCTTCACTGTCCGCCCCGGAACCTCCCAGCCCATCACCCTACAGAGCCGGCC CCCTGACTATGAAAGCAGGACCTCAGGACCGAGACGTGCCCCAAGCCCTGTGGTTTCGCCAACGGAATTG AGCAAAGAGATTCTGCCCACCCCTCCGTCTGCTGCAGCAGCCTCTCCCTCCCCCACACTCTCAGATGTCT TTAGCCTTCCAAGCCAGTCCCCTGCAGGGGACCTCTTTGGCTTGAACCCAGCAGGACGCAGCAGGTCACC ATCTCCTTCAATATTGCAACAGCCAATCTCAAATAAGCCTTTTACAACTAAGCCTGTCCACCTGTGGACG AAACCAGATGTGGCAGACTGGCTGGAAAGTCTGAACTTGGGTGAACACAAGGAGACGTTCATGGACAATG AGATTGACGGCAGCCATCTGCCAAACCTCCAGAAGGAAGACTTGATAGATCTTGGGGTGACTCGAGTTGG GCACAGGATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGACAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR200193 representing NM_001004133 Red=Cloning site Green=Tags(s) MMSVPGGGAATVMMTGYNNGRYPRNSLYSDCIIEDKTVVLQKKDNEGFGFVLRGAKADTPIEEFTPTPAF PALQYLESVDEGGVAWQAGLRTGDFLIEVNNENVVKVGHRQVVNMIRQGGNHLVLKVVTVTRNLDPDDTA RKKAPPPPKRAPTTALTVRSKSMTAELEELVDKDKPEEIVPASKPSRTAENVAIESRVATIKQRPTSRCF PAASDVNSVYERQGIAVMTPTVPGSPKGPFLGLPRGTMRRQKSIDSRIFLSGITEEERQFLAPPMLKFTR SLSMPDTSEDIPPPPQSVPPSPPPPSPTTYNCPRSPTPRVYGTIKPAFNQNPVAKVPPATRSDTVATMMR EKGMFYRRELDRFSLDSEDVYSRSPAPQAAFRTKRGQMPENPYSEVGKIASKAVYVPAKPARRKGMLVKQ SNVEDSPEKTCSIPIPTIIVKEPSTSSSGKSSQGSSMEIDPQATEPGQLRPDDSLTVSSPFAAAIAGAVR DREKRLEARRNSPAFLSTDLGDEDVGLGPPAPRMQPSKFPEEGGFGDEDETEQPLLPTPGAAPRELENHF LGGGEAGAQGEAGGPLSSTSKAKGPESGPAAALKSSSPASPENYVHPLTGRLLDPSSPLALALSARDRAM QESQQGHKGEAPKADLNKPLYIDTKMRPSVESGFPPVTRQNTRGPLRRQETENKYETDLSKDRRADDKKN MLINIVDTAQQKSAGLLMVHTVDIPVAGPPLEEEEDREDGDTKPDHSPSTVPEGVPKTEGALQISAAPEP AAAPGRTIVAAGSVEEAVILPFRIPPPPLASVDLDEDFLFTEPLPPPLEFANSFDIPDDRAASVPALADL VKQKKSDTPQPPSLNSSQPANSTDSKKPAGISNCLPSSFLPPPESFDAVTDSGIEEVDSRSSSDHHLETT STISTVSSISTLSSEGGESMDTCTVYADGQAFVVDKPPVPPKPKMKPIVHKSNALYQDTLPEEDTDGFVI PPPAPPPPPGSAQAGVAKVIQPRTSKLWGDVTEVKSPILSGPKANVISELNSILQQMNRGKSVKPGEGLE LPVGAKSANLAPRSPEVMSTVSGTRSTTVTFTVRPGTSQPITLQSRPPDYESRTSGPRRAPSPVVSPTEL SKEILPTPPSAAAASPSPTLSDVFSLPSQSPAGDLFGLNPAGRSRSPSPSILQQPISNKPFTTKPVHLWT KPDVADWLESLNLGEHKETFMDNEIDGSHLPNLQKEDLIDLGVTRVGHRMNIERALKQLLDR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001004133
ORF Size	3756 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001004133.1, NP_001004133.1
RefSeq Size	4636 bp
RefSeq ORF	3759 bp
Locus ID	171093
UniProt ID	Q9QX74
MW	134.3 kDa
Gene Summary	binds to cortactin and alpha-latrotoxin receptor and is involved in the structural and functional organization of the post synaptic density [RGD, Feb 2006]

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