Rtn3 (NM_080909) Rat Tagged ORF Clone
CAT#: RR200211
- TrueORF®
Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 1, (10 ug)
"NM_080909" in other vectors (3)
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CNY 12,070.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200211 representing NM_080909
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGTCATCGGCGGCCACTCAGTCTCCGTCAGTCTCCTCGTCGTCCTCCGGGGCCGAGCCGTCAA CGCTTGGCGGCGGCGGCGGGAGCCCTGGAGCCTGCCCCGCCCTGGGGGCGAAGAGCTGCGGCTCCTCCTG TGCGGTGGGATTGAGCTCTCTTTGCTCTGATGAGCCACCATCAAAAATTATGACTTCCTCCTTTCTTTCA TCTTCTGAAATATATAACCCTGACCCTACAACACCACTTGGAAGTGAAACATTAGGCAGCCATCTTGTAG CTAAAGACGGAAAAGATCCCTTGGTTCTTCTAGATAAGAAAACTCTGGACTCACCTCAGGGGACCAACAA GGACAGAGTAGACACTCCGGTTTCTCTTGCAGCTGACATTCCTTGCAGCCACCCTTCTATTCCAGACAGT TTCCCAGAACAACCTACTTTTCTTTCAAAGGAAACTGATCCAACAGAAGAGTGGGTAGTTAAAAACCAAG AACCCAAGAACCCAAGTGAAGTTCCAAGTGGAGAGGACAGAAGTGCACTGGATCTTGGGCAGCGTAAGGC AGAACACATTTGTACACATTCCTTGTCTCCATCTGAACCTGCAGTAGCCAGTGTAGAAAAAGATTCTCCT GAGTCACCATTTGAAGTAATTATTGACAAAGCAACATTTGACAGAGAATTTAAAGATTTGTATAAGGAAA GCACAGATGATTTGGGTGGCTGGGCAGCTCACGGTGACAGAGAATCCCCAGCAGATTTATTGGAAATGAA TGACAAAGTGTTTCCACTGAGAAATAAAGAGGCAGGGCGTTACCCATCCTCTGCACTGCTCGGTAGACAA TTCTCACACACTACAGCAGCACTGGAAGAAGTGTCTAGATGTGTGAATGATATGCATAACTTTACTAATG AAATACTGACTTGGGACTTAGATCCCCAAGCAAAACAACAGGCGGACAAATCACCTTGCACCACAGAAAG TACAGGACTAGACAGGAGTGAACATCGCTCAGAAATTCCAGTTATAAATCTTAAAACAAGCCCTCAACAG AAAATGCCTGTATGTTCTTTTAATGGGAGCACTCCCATTACTAGATCCACAGGTGACTGGACAGAAACAT TTACAGAAGGAAAACCTGTAAAAGGCTACCTCAGCTCCACAAAAGAAGCTGGTGGCAAAGGTGTACCAGA CAGTTCTCAGCCTATCTCAGGCTCTGGAGCAGCCACAGTGGAAGTGACTTTACCTGACCTGAGGGGTACC TGGCCTAACTCTGTGCTAGGGGAAGTCACAGAGGTTGATAGTTCTGGGGAATCTGATGACACAGTAATAG AGGACACCACAGAAAATCCCTCAGTTAAGAACAACAAAGTTTTGCCAGAAAAATCTGACTCCCTTCCAAG TGCTGCTGTAAAAACAAGTGAAAGGGAAAACAAAGAGACTACCAGTCATGAAACTGTGAGGAGTGAAATG TATGAAAACTCTGAGCAGCAGCAGGCCCATGCAGAAACTCCTACTCAGAGGAGTCTGGAAGGTCAGATGT CTCCACAAGTACCTAATATGCTGAATGAAGTCATACCTGAAAATCTTGCTATGACTGACACAACCAGAGT TTGCTCAGCAGCACCTCCAAGTGTTCTTAGTGAGACTGGATTCTCACTAACTGTGCCAGCTTCTGCCAAG TCAGAATCTTTGCTTGGAAAAAATGTTGAAGATACAGATGGTTCCTCTCCAGAGGACTTGATGGCTGCCC TCACAGGAACTGAGGAGAAGGGGACAGTGGATAAAGAGAAAGGTGATGTTTTGGAAGCAGTGTTAGAGAA GATAGCAAACGTTAAAAACACTTTGCCTGTGGAACTCTTGCATGAAAATAAGCTAAGTAGTTCTGAAACC AAAAATACTAAAAGCAAATACAGTGAACACAGCAGAGAAACAAATGGAGGTGCCCCCAAGGTGTCTTCCG ACTTAGAGCAGGAGCAGCTCACCATTAGAGCCATTAAAGAATTAGGGGCTAAGCAGGTGCAGGCTGAAAG AATGTCTCCTGGAGGAAAACTCAAGCGAACCTTTGATCCACAGTCTGGGCCACAGAATTCATCTGACATC CTAGAACACACAGATATCACAACTGGATCTGATCTTGGGATTCCCAAAAAGCCTACTATCATCAAAGACA CTAGAATAGATTCTATTTCCAGCCTTACCAAGACTGAAACAGTTAACAAGCATGTTCTAGCAAGACTTCT CAGTGATTTCCCAGTGCATGATCTGATTTTCTGGCGAGATGTGAAGAAGACTGGGTTTGTCTTTGGCACC ACGCTGATCATGCTGCTCTCTCTGGCAGCTTTCAGTGTTATCAGTGTGGTCTCTTACCTCATCCTGGCTC TACTCTCTGTCACCATCAGCTTCAGAGTCTACAAGTCTGTCATCCAAGCTGTGCAGAAGTCAGAAGAAGG ACATCCATTCAAGGCCTACCTGGATGTGGACATTACACTGTCCTCAGAAGCTTTCCACAGCTACATGAAT GCTGCAATGGTGCATGTCAACAAGGCCCTCAAACTCATTATTCGTCTCTTTCTGGTAGAAGACTTGGTTG ACTCCTTGAAGCTGGCTGTCTTCATGTGGCTGATGACCTACGTCGGTGCTGTTTTTAACGGAATTACCCT TCTGATTCTCGCCGAGCTGCTGGTTTTCAGCGTCCCAATTGTCTATGAGAAGTATAAGACACAGATTGAC CACTATGTTGGGATTGCCCGGGATCAGACCAAGTCAATTGTTGAAAAGATCCAAGCAAAGCTTCCTGGAA TCGCCAAAAAAAAGGCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200211 representing NM_080909
Red=Cloning site Green=Tags(s) MAESSAATQSPSVSSSSSGAEPSTLGGGGGSPGACPALGAKSCGSSCAVGLSSLCSDEPPSKIMTSSFLS SSEIYNPDPTTPLGSETLGSHLVAKDGKDPLVLLDKKTLDSPQGTNKDRVDTPVSLAADIPCSHPSIPDS FPEQPTFLSKETDPTEEWVVKNQEPKNPSEVPSGEDRSALDLGQRKAEHICTHSLSPSEPAVASVEKDSP ESPFEVIIDKATFDREFKDLYKESTDDLGGWAAHGDRESPADLLEMNDKVFPLRNKEAGRYPSSALLGRQ FSHTTAALEEVSRCVNDMHNFTNEILTWDLDPQAKQQADKSPCTTESTGLDRSEHRSEIPVINLKTSPQQ KMPVCSFNGSTPITRSTGDWTETFTEGKPVKGYLSSTKEAGGKGVPDSSQPISGSGAATVEVTLPDLRGT WPNSVLGEVTEVDSSGESDDTVIEDTTENPSVKNNKVLPEKSDSLPSAAVKTSERENKETTSHETVRSEM YENSEQQQAHAETPTQRSLEGQMSPQVPNMLNEVIPENLAMTDTTRVCSAAPPSVLSETGFSLTVPASAK SESLLGKNVEDTDGSSPEDLMAALTGTEEKGTVDKEKGDVLEAVLEKIANVKNTLPVELLHENKLSSSET KNTKSKYSEHSRETNGGAPKVSSDLEQEQLTIRAIKELGAKQVQAERMSPGGKLKRTFDPQSGPQNSSDI LEHTDITTGSDLGIPKKPTIIKDTRIDSISSLTKTETVNKHVLARLLSDFPVHDLIFWRDVKKTGFVFGT TLIMLLSLAAFSVISVVSYLILALLSVTISFRVYKSVIQAVQKSEEGHPFKAYLDVDITLSSEAFHSYMN AAMVHVNKALKLIIRLFLVEDLVDSLKLAVFMWLMTYVGAVFNGITLLILAELLVFSVPIVYEKYKTQID HYVGIARDQTKSIVEKIQAKLPGIAKKKAE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_080909 |
ORF Size | 2820 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_080909.3, NP_543185.2 |
RefSeq Size | 4926 bp |
RefSeq ORF | 2823 bp |
Locus ID | 140945 |
UniProt ID | Q6RJR6 |
MW | 101.5 kDa |
Gene Summary | This gene encodes a member of the reticulon family, a large group of membrane-bound proteins that primarily localize to the endoplasmic reticulum with a small subpopulation at the cell surface. These proteins play roles in endocytosis, exocytosis, trafficking and neuronal outgrowth. Proteins belonging to this family are characterized by a carboxy-terminal reticulon homology domain that consists of a small hydrophilic loop flanked by hydrophobic regions. In rat, this protein has been shown to interact with two members of the synaptic adhesion-like molecule family, suggesting that it may function in trafficking of these proteins. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200211 | Rtn3 (untagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 1, (10 ug) |
CNY 12,070.00 |
|
RR200211L3 | Lenti ORF clone of Rtn3 (Myc-DDK-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 1, (10 ug) |
CNY 13,780.00 |
|
RR200211L4 | Lenti ORF clone of Rtn3 (mGFP-tagged ORF) - Rat reticulon 3 (Rtn3), transcript variant 1, (10 ug) |
CNY 15,200.00 |