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Hap1 (NM_024133) Rat Tagged ORF Clone
CAT＃: RR200387
- TrueORF®
Hap1 (Myc-DDK-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 1, (10 ug)
"NM_024133" in other vectors (3)
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CNY 7,700.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HAP1-A; HAP1-B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200387 representing NM_024133
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCCCGAAGGACCAGGTGCAGAGCAGTGCCGGGGACGGGACGGGGTCGGGGGACCCAGCAACAGGCA CCCCCACGACCCAGCCTGCAGCGGATCCCGCTCCGGAGCCCTCGGCAGAGCCCAAACCTGCTCCGGCGCA GGGAACCGGGTCCGGACAGAAATCAGGATCCCGAACCAAGACAGGAGGAAGCTTTTGTAGGTCCAGGATC CGTGGCGACTCGGACGCACCATGGACCCGCTACATATTCCAGGGGCCTTACGGTCCCCGGGCTACTGGCC TGGGCACTGGAAGGGCTGAGGGAATCTGGAAGACGCCAGCCGCGTACATCGGCCGAAGGCCCGGCGTGTC CGGCCCTGAGCGCGCGGCGTTTATTCGAGAGCTGCAGGAAGCGCTGTGTCCTAATCCACTGCCCAGGAAG AAGATCACCGAAGATGATATCAAAGTGATGTTGTATTTGCTGGAAGAGAAAGAACGGGACCTGAACACAG CCGCTCGCATCGGCCAGTCCCTGGTGAAACAGAATAGTGTTCTGATGGAGGAGAATAACAAGCTGGAAAC CATGCTGGGCTCAGCCAGAGAGGAGATTTTACATCTCCGGAAGCAGGTGAACCTGCGAGATGATCTCCTT CAGCTCTACTCGGACTCCGATGACGATGAGGAGGATGAAGAGGATGAGGAAGAGGAAGAGGGAGAAGAGG AGGAACGAGAAGGACAGAGGGACCAAGATCAGCAGCACGACCATCCCTATGGTGCCCCCAAGCCGCCCCC TAAGGCTGAGACGCTGCACCACTGCCCACAGCTGGAAGCCCTGAAGCAGAAGCTGAAACTGCTGGAAGAA GAGAACGACCATCTTCGAGAGGAGGCCTCCCACCTTGACAACCTGGAAGACAAAGAACAGATGCTCATTC TGGAGTGTGTGGAACAGTTTTCTGAAGCCAGCCAGCAGATGGCAGAGCTATCCGAGGTGTTGGTGCTGAG GCTGGAAGGCTATGAGAGGCAGCAGAAGGAGATCACTCAGCTGCAGGCCGAGATCACCAAGCTACAACAG CGTTGTCAGTCTTATGGGGCCCAGACGGAGAAACTGCAGCAGCAGCTGGCCTCAGAGAAGGGAGTCCACC CAGAGAGCCTGCGAGCTGGCTCCCACATGCAGGATTATGGAAGCAGGCCTCGTGAACGCCAGGAGGATGG GAAGAGCCATCGTCAGCGTTCCTCAATGCCTGCAGGTTCTGTCACCCACTATGGATACAGTGTGCCTCTG GATGCACTTCCAAGTTTCCCAGAGACACTGGCGGAGGAGCTCCGGACATCCCTGAGGAAGTTCATCACTG ACCCTGCGTATTTCATGGAGAGATGTGACACTCGCTGCAGAGAGGAACGAAAGAAGGAGCAGGGGACAAT GCCACCCCCACCGGTGCAAGATCTCAAGCCGCCTGAAGATTTCGAGGCTCCAGAGGAGCTGGTTCCTGAG GAGGAGCTGGGGGCCATAGAAGAGGTGGGGACAGCTGAGGATGGGCCGGCAGAAGAGACAGAGCAGGCAT CTGAGGAGACCGAGGCCTGGGAGGAGGTGGAACCGGAGGTGGACGAGGCCACAAGGATGAATGTGGTGGT CTCTGCCCTGGAGGCCAGCGGCCTGGGCCCTTCACACCTGGACATGAAGTATGTCCTCCAGCAACTGTCC AACTGGCAGGACGCCCATTCTAAGCGGCAGCAGAAGCAGAAGGTGGTCCCGAAAGGTGAGTGTTCCCGCA GAGGACACCCTCCTGCCAGTGGGACAAGCTACCGATCATCAACCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200387 representing NM_024133
Red=Cloning site Green=Tags(s) MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRI RGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRK KITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLL QLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEE ENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQ RCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPL DALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPE EELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLS NWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSYRSSTL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_024133 |
| ORF Size | 1797 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_024133.3 |
| RefSeq Size | 3691 bp |
| RefSeq ORF | 1800 bp |
| Locus ID | 29430 |
| UniProt ID | P54256 |
| MW | 67 kDa |
| Gene Summary | Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that is homologous to the human huntingtin-associated protein 1. The human protein interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Two transcripts encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200387 | Hap1 (untagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 1, (10 ug) |
CNY 7,700.00 |
|
| RR200387L3 | Lenti ORF clone of Hap1 (Myc-DDK-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 1, (10 ug) |
CNY 9,600.00 |
|
| RR200387L4 | Lenti ORF clone of Hap1 (mGFP-tagged ORF) - Rat huntingtin-associated protein 1 (Hap1), transcript variant 1, (10 ug) |
CNY 10,450.00 |
