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Gnas (NM_021845) Rat Tagged ORF Clone
CAT＃: RR200466
- TrueORF®
Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug)
"NM_021845" in other vectors (3)
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CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ALEX; G-alpha-8; Gnas1; Gnpas; Nesp55; SCG6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200466 representing NM_021845
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCATGCTCAACTGCCTCCACGGCAATAATATGTCAGGACAACACGATATCCCCCCTGAAGTCGGGG ACCAGCCCGAGCAAGAACCTTTGGAAGCCCAAGGGGCCGCTGCCCCCGGTGCTGGGGTTGGCCCAGCCGA AGAAATGGAGACCGAACCGTCTAACAATGAGCCCATCCCCGACGAGACTGACAGTGAGGTCTGTGGACCC CCTGAAGACTCCAAATCTGACATCCAGAGCCCCAGTCAGGCCTTCGAGGAAGTCCAAGTGGGCGGAGACT ACAGCCCACCTCCAGAGGAAGCCATGCCATTCGAGATCCAACAGCCCAGCCTGGGAGATTTCTGGCCCAC CCTGGAGCAGCCTGGACCATCTGGGACCCCATCAGGCATCAAAGCCTTCAACCCAGCGATTTTGGAGCCC GGGACCCCCACTGGTGCACACCCAGGCCTGGGAGCCTATAGCCCCCCACCAGAAGAAGCTATGCCATTTG AGTTCAATGAGCCTGCCCAGGAAGACCGTTGCCAGCCTCCCTTGCAAGTCCCAGACCTTGCGCCAGGGGG TCCGGAAGCATGGGTCTCCAGAGCTCTTCCCGCGGAGCCCGGGAACCTCGGATTTGAAAACACTGGCTTC CGAGAAGACTACAGCCCTCCACCTGAAGAATCTGTGCCATTTCAGCTCGATGGAGAAGAATTCGGGGGCG ATAGCCCACCCCCAGGACTCCCGCGAGTCACGCCACAAATCGGCATTGGCGGCGAGTTCCCGACAGTCGC GGTCCCGAGTACGCTCTGCCTCGCTCCCGCCGCGAACGCGCCTCCCCTCTGGGTCCAGGGCGCCATTGGC AGACCATTCCGCGAGGCTGTCAGATCTCCTAACTTCGCATACGACATTTCCCCAATGGAGATCACCAGAC CCCTGCTTGAGATTGGCAGAGCCTCCACTGGGGTCGACGACGACACCGCTGTCAATATGGACAGCCCCCC AATCGCAAGTGATGGCCCGCCCATCGAAGTCTCGGGAGCCCCAGTTAAGAGCGAGCACGCAAAGAGACCC CCACTTGAGCGACAAGCAGCCGAGACAGGAAACAGCCCTATCAGCAGCACCACTGCAGAGGAGGCAAAAG TCCCCTCCCTCGAGCGAGGAGAAGGATCCCCCACCCAACCTGAAACAGTGCATATCAAGCCAGCTCCTGT TGCGGAGTCCGGAACGGACTCTTCCAAAGCCGATCCGGACTCAGCTACACACGCAGTTCTTCAGATCGGT CCTGAGGAAGTCGGAGGAGTCCCAACCATGCCCACCGATCTTCCGCCTGCTTCTGAAGATGCGGGCCCAG ATGTCCGGGCAGAACCAGACGGAGGGACAGCCCCAGCCACCCCTGCCGAGTCCGAAGACAACAGAGAACC AGCCGCCGCCGCCGCCGCCGAGCCAGCCGCCGAGCCAGCCGCTGAGCCAGCCGCCGAGCCAGCCGCCGAG CCAGCCGCCGAGCCAGCTGCCGAGGCAGTCCCTGACACCGAAGCCGAGTCTGCCTCCGGGGCAGTCCCCG ACACCCAAGAGGAGCCCGCAGCCGCGGCAGCCTCTGCCACGCCGGCGGAGCCTGCCGCCCGGGCAGCCCC CGTCACCCCTACGGAGCCCGCTACCCGGGCTGTCCCTTCTGCCAGAGCCCATCCAGCCGCCGGGGCTGTC CCTGGAGCCTCAGCGATGTCAGCCGCTGCTAGGGCAGCCGCCGCTAGAGCAGCCTATGCAGGTCCTCTGG TCTGGGGAGCCAGGTCACTCTCGGCTACTCCAGCCGCTCGGGCATCCCTCCCTGCCCGCGCAGCAGCTGC CGCCCGAGCAGCCTCTGCTGCCCGCGCAGTCGCTGCCGGCCGGTCAGCCTCTGCCGCCCCAAGCAGGGCC CATCTTAGACCCCCCAGCCCGGAGATCCAGGTTGCTGACCCGCCTACTCCGCGGCCTGCTCCGCGGCCGA GTGCCTGGCCTGACAAATACGAACGTGGCCGAAGCTGCTGCAGGTATGAGGCTGCGTCCGGCATCTGCGA GATCGAGTCCTCCAGCGATGAGTCGGAAGAAGGGGCCACTGGCTGCTTCCAGTGGCTTCTGCGGCGAAAC CGCCGCCCTGGCCAGCCCCGGAGCCACACAGTCGGGAGCAACCCGGTCCGCAACTTCTTCGCCCGAGCCT TCGGAAGCTGCTTCGGTCTATCTGAGTGTACCCGATCACGATCCCTCAGCCCCGGGAAGGCCAAGGATCC TATGGAAGAGAGGCGCAAACAGATGCGCAAAGAAGCCATGGAGATGCGAGAGCAGAAGCGCGCAGATAAG AAACGCAGCAAGCTCATCGACAAGCAACTGGAGGAGGAGAAGATGGACTACATGTGTACACACCGCCTGC TGCTTCTAGGTGCTGGAGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTACATGTTAATGG GTTTAACGGAGAGGGCGGCGAAGAGGACCCGCAGGCTGCAAGGAGCAACAGCGATGGTGAGAAGGCCACC AAAGTGCAGGACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGG TGCCCCCCGTGGAGCTGGCCAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGT GCCAAACTTTGACTTCCCACCTGAATTCTATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTTCGT GCCTGCTACGAGCGCTCCAACGAGTACCAGCTGATCGACTGTGCCCAGTACTTCCTGGACAAGATTGATG TGATCAAGCAGGCCGACTACGTGCCAAGTGACCAGGACCTGCTTCGCTGCCGCGTCCTGACCTCTGGAAT CTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCCAGCGCGATGAA CGCCGCAAGTGGATCCAGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTACA ACATGGTCATCCGGGAGGACAACCAGACCAACCGTCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTG GAACAACAGATGGCTGCGTACCATCTCTGTGATCCTCTTCCTCAACAAGCAAGATCTGCTTGCTGAGAAG GTCCTCGCTGGGAAATCGAAGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACCACTCCTGAGGATG CGACTCCCGAGCCCGGAGAGGACCCACGCGTGACCCGGGCCAAGTACTTCATCCGGGATGAGTTTCTGAG AATCAGCACTGCTAGTGGAGATGGACGTCACTACTGCTACCCTCACTTTACCTGCGCCGTGGACACTGAG AACATCCGCCGTGTCTTCAACGACTGCCGTGACATCATCCAGCGCATGCATCTTCGCCAATACGAGCTGC TC ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA >RR200466 representing NM_021845
Red=Cloning site Green=Tags(s) MGMLNCLHGNNMSGQHDIPPEVGDQPEQEPLEAQGAAAPGAGVGPAEEMETEPSNNEPIPDETDSEVCGP PEDSKSDIQSPSQAFEEVQVGGDYSPPPEEAMPFEIQQPSLGDFWPTLEQPGPSGTPSGIKAFNPAILEP GTPTGAHPGLGAYSPPPEEAMPFEFNEPAQEDRCQPPLQVPDLAPGGPEAWVSRALPAEPGNLGFENTGF REDYSPPPEESVPFQLDGEEFGGDSPPPGLPRVTPQIGIGGEFPTVAVPSTLCLAPAANAPPLWVQGAIG RPFREAVRSPNFAYDISPMEITRPLLEIGRASTGVDDDTAVNMDSPPIASDGPPIEVSGAPVKSEHAKRP PLERQAAETGNSPISSTTAEEAKVPSLERGEGSPTQPETVHIKPAPVAESGTDSSKADPDSATHAVLQIG PEEVGGVPTMPTDLPPASEDAGPDVRAEPDGGTAPATPAESEDNREPAAAAAAEPAAEPAAEPAAEPAAE PAAEPAAEAVPDTEAESASGAVPDTQEEPAAAAASATPAEPAARAAPVTPTEPATRAVPSARAHPAAGAV PGASAMSAAARAAAARAAYAGPLVWGARSLSATPAARASLPARAAAAARAASAARAVAAGRSASAAPSRA HLRPPSPEIQVADPPTPRPAPRPSAWPDKYERGRSCCRYEAASGICEIESSSDESEEGATGCFQWLLRRN RRPGQPRSHTVGSNPVRNFFARAFGSCFGLSECTRSRSLSPGKAKDPMEERRKQMRKEAMEMREQKRADK KRSKLIDKQLEEEKMDYMCTHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKAT KVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPNFDFPPEFYEHAKALWEDEGVR ACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDE RRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEK VLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTE NIRRVFNDCRDIIQRMHLRQYELL TRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-NotI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_021845 |
| ORF Size | 3432 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_021845.5, NP_068617.6 |
| RefSeq Size | 3826 bp |
| RefSeq ORF | 3435 bp |
| Locus ID | 24896 |
| MW | 122.9 kDa |
| Gene Summary | This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene. [provided by RefSeq, Apr 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200466 | Gnas (untagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 9,120.00 |
|
| RR200466L3 | Lenti ORF clone of Gnas (Myc-DDK-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 10,930.00 |
|
| RR200466L4 | Lenti ORF clone of Gnas (mGFP-tagged ORF) - Rat GNAS complex locus (Gnas), transcript variant 1, (10 ug) |
CNY 12,070.00 |
