Akr7a2 (NM_134407) Rat Tagged ORF Clone

CAT#: RR200557

  • TrueORF®

Akr7a2 (Myc-DDK-tagged ORF) - Rat aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase) (Akr7a2), (10 ug)



  "NM_134407" in other vectors (3)

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CNY 3,990.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Rat Tagged ORF Clone
Tag Myc-DDK
Synonyms Aiar
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RR200557 representing NM_134407
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGGTCTCCGGCACCCCGCGCCGTCTCTGGCGCCCCTCTCCGGCCCGGTACGGTGCTGGGCACCA
TGGAGATGGGGCGCCGCATGGATGCGAGTGCTAGCGCTGCGACCGTACGCGCCTTCCTGGAGCGCGGCCT
CAACGAGCTGGACACGGCCTTCATGTATTGCGACGGCCAGTCCGAAAGCATCCTGGGCAGCCTGGGGCTC
GGGCTGGGCAGCGGCGACTGCACAGTGAAAATTGCCACCAAGGCCAACCCTTGGGACGGGAAGTCGCTGA
AGCCTGACAGTGTCCGGTCCCAATTAGAGACGTCATTGAAGCGGCTGCAGTGTCCCCGGGTGGACCTCTT
CTACTTACACGCTCCTGACCACGGCACTCCTATCGTGGAGACCCTGCAGGCCTGCCAACAGCTGCATCAG
GAGGGCAAGTTTGTGGAGCTTGGCTTGTCCAACTATGCCTCCTGGGAGGTAGCCGAGATCTATACCCTCT
GTAAAAGCAACGGCTGGATCCTGCCAACTGTGTACCAGGGCATGTACAACGCCACCACCCGGCAGGTGGA
GACTGAGCTCCTCCCCTGCCTCAGATACTTCGGACTGAGGTTCTATGCCTACAACCCTTTGGCTGGAGGC
CTGCTGACTGGCAAATACAGATATGAAGACAAGGATGGGAAACAGCCCGAGGGCCGCTTCTTTGGGAATA
GCTGGTCTGAGACCTACAGGAACCGCTTCTGGAAGGAACACCACTTTGAGGCCATTGCCCTGGTAGAAAA
GGCCCTGAAGACCACCTATGGCACCAGTGCCCCCAGCATGACCTCGGCTGCCCTGCGCTGGATGTACCAT
CACTCACAGCTCCAGGGCACCCGAGGGGACGCAGTCATCTTGGGCATGTCCAGCCTGGAGCAGCTGGAGC
AGAACTTGGCGGCCACTGAGGAAGGTCCCCTGGAGCCCGCTGTCGTGGAGGCCTTTAACCAAGCCTGGAA
CGTGGTCGCCCACGAGTGTCCCAACTACTTCAGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RR200557 representing NM_134407
Red=Cloning site Green=Tags(s)

MSRSPAPRAVSGAPLRPGTVLGTMEMGRRMDASASAATVRAFLERGLNELDTAFMYCDGQSESILGSLGL
GLGSGDCTVKIATKANPWDGKSLKPDSVRSQLETSLKRLQCPRVDLFYLHAPDHGTPIVETLQACQQLHQ
EGKFVELGLSNYASWEVAEIYTLCKSNGWILPTVYQGMYNATTRQVETELLPCLRYFGLRFYAYNPLAGG
LLTGKYRYEDKDGKQPEGRFFGNSWSETYRNRFWKEHHFEAIALVEKALKTTYGTSAPSMTSAALRWMYH
HSQLQGTRGDAVILGMSSLEQLEQNLAATEEGPLEPAVVEAFNQAWNVVAHECPNYFR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_134407
ORF Size 1014 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_134407.1, NP_599234.1
RefSeq Size 1272 bp
RefSeq ORF 1017 bp
Locus ID 171445
UniProt ID Q8CG45
MW 37.6 kDa
Gene Summary Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. Acts as a 2-carboxybenzaldehyde reductase.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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