United States
Germany
Japan
United Kingdom
China
Cd300lf (NM_001025111) Rat Tagged ORF Clone
CAT＃: RR200569
- TrueORF®
Cd300lf (Myc-DDK-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug)
"NM_001025111" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD300f; RGD1309733 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200569 representing NM_001025111
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCATTTGTCATTGCTGGCTCTCTTTCTCTTCTGGATCTCAGGCTGCTTCACTGCTCAGGATCCAGTCA CAGGTCCAGAGGAGGTGAGCGGTTACGAGCAGGGCTCCCTGACAGTGTGGTGTCGATATGGCTCATGGTG GAAGGATTACAGCAAGTACTGGTGCCGAGGACCTAAGAGGAGTTCCTGTGAGATTCGAGTTGAAACCGAT GCATCAGAGCGCTTGGTGAAGGAGAACCATGTGTCCATCAGGGACGACCAGACAAACTTCACCTTCACAG TGACCATGGAGGACCTGAGGATGAGCGATGCTGGCATTTACTGGTGTGGAATTACAAAGGCTGGATATGA TCACATGTTTAAAGTTCATGTGAGCATTAACCCAGTGCCAACAACGCCCACCACCACCTCCACCACCACC ATCTTCACAGTGACAACCACTGTAAAAGAGACCAGCACGCTTTCAACGCAGACCAGCCACTACTCTGATA ACAGGTATGACAGTGGTGGTGTCGGCGACGGTAATGGGTTTCTGGATCTCAGCGTGCTCCTCCCAGTCAT CTCTGCAGCCCTGTTGCTTCTCTTGCTGGTGGTCTCACTAATTGCTTGGAGGATGGTGAGGAGACAGAAG AAAGCTGCTGGACCACCCTCAGGGCAGGCTGATTCTGTGACTCACATCTTGTGTCCCCCCAAACCCCATG TACAGCCTCTGGAGGATGATCTCTGTTATGCAAACCTGTCACTGCAGCAGCCCAGAACCTCCCCTTTGAA AAAGGGCTCCTCCATGTCCTCCTCTGGCAAGGACCACCAAGAGGAAGTGGAATACGTCACCATGGCTCCC TTCCCTAGGGAGGAGATTTCATACGCTGCTCTGTCTTTGGCGAGCTTGGGTCAGGAACCTACTTACAGCA ACACTGCCTGCCTTGTCACCCATGGTCCCAGGACAAACCTTGGAGAGGAGACCACAGAGTACAGCAGCAT CAGGAGGCCCATGCCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200569 representing NM_001025111
Red=Cloning site Green=Tags(s) MHLSLLALFLFWISGCFTAQDPVTGPEEVSGYEQGSLTVWCRYGSWWKDYSKYWCRGPKRSSCEIRVETD ASERLVKENHVSIRDDQTNFTFTVTMEDLRMSDAGIYWCGITKAGYDHMFKVHVSINPVPTTPTTTSTTT IFTVTTTVKETSTLSTQTSHYSDNRYDSGGVGDGNGFLDLSVLLPVISAALLLLLLVVSLIAWRMVRRQK KAAGPPSGQADSVTHILCPPKPHVQPLEDDLCYANLSLQQPRTSPLKKGSSMSSSGKDHQEEVEYVTMAP FPREEISYAALSLASLGQEPTYSNTACLVTHGPRTNLGEETTEYSSIRRPMP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001025111 |
| ORF Size | 996 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001025111.1, NP_001020282.1 |
| RefSeq Size | 1818 bp |
| RefSeq ORF | 999 bp |
| Locus ID | 287818 |
| UniProt ID | Q566E6 |
| MW | 36.8 kDa |
| Gene Summary | Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200569 | Cd300lf (untagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 3,990.00 |
|
| RR200569L3 | Lenti ORF clone of Cd300lf (Myc-DDK-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 6,080.00 |
|
| RR200569L4 | Lenti ORF clone of Cd300lf (mGFP-tagged ORF) - Rat CD300 molecule-like family member f (Cd300lf), (10 ug) |
CNY 6,650.00 |
其它Cd300lf产品
