Gpr135 (NM_181771) Rat Tagged ORF Clone
CAT#: RR200777
- TrueORF®
Gpr135 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 135 (Gpr135), (10 ug)
"NM_181771" in other vectors (3)
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CNY 3,656.00
CNY 5,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR200777 representing NM_181771
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGCAGGCGCGGCCGCCGAGCCGCCCGGCTGCGAGTGCAACCTTGCCGGGTAGCGCACATCCCG GAGGGGCGGCTTCCACGGCCACCGCGGCCGCGCTGTCGTTCAGCTCCGTAGCCACCGTAACGCTGGGAAA CCAGAGCGATGCGGGCAGGCCGGAGGCGGCTGGGTCTCGGGGACCGGCACCGCTGCTTTGGCACGGGGCA GCAGTGGCCGCCCAGGCGCTCGTGCTCCTGCTCATCTTCTTACTGTCTAGCCTGGGAAACTGCGCGGTGA TGGGGGTGATCGTGAAGCACCGGCAGCTGCGCACGGTCACCAACGCCTTCATCCTATCGCTGTCCCTGTC GGACCTGCTCACTGCGCTGCTCTGCTTACCCGCCGCCTTCCTCGACCTGTTTGCGCCGCCGGGAGACTCG GGGCCCTGGCGCAGCTTCTGCGCCGCCAGCCGCTTCTTCAGTTCGTGTTTCGGCATCGTCTCCACGTTCA GCGTGGCGCTCATCTCGCTGGACCGCTACTGCGCCATCGTGCGGCCGCCGCGGGACAAGCTAGGCCGGCG GCGCGCGCTGCAGCTGCTGGCCGGAGCGTGGCTGGCTGCGCTCGGCTTCTCCCTGCCCTGGGAGCTGCTC CGGGCACCCCGGGAGCCCCCGACTCCGCAGAGCTTCCACCGCTGCCTTTACAGAACCTCCCCAGACCCTG CGCAGCTGGGCGCCGCTTACAGCGTGGGGCTGGTGGTGGCTTGCTACCTGCTGCCCTTCCTGCTGATGTG TTTCTGCCGCTACCACATCTGCAAGACTGTGCGCCTGTCGGACGTGCGTGTGCGGCCGATGACCACCTAT GCGCGCGTGCTGCGCTTTTTCAGCGAGGTGCGCACGGCCACCACCGTGCTCATCATGATTGTCTTTGTCA TCTGCTGCTGGGGCCCCTACTGCTTCCTGGTGTTGTTGGCTGCTACCCGGCAGGGTCAGACCACACAGGC TCCCTCGCTGCTCAATGTGGCAGCTGTTTGGCTGACCTGGGCCAATGGAGCTATCAACCCGGTCATATAT GCCATCCGCAACCCTAACATTTCTATGTTCCTAGGTCGCAACCGCGAAGAGGGATATAGGACTAGAAACA TGGATGTTTTTTTGCCTAGCCAAGGCCTAGGTTTTCAGGCCAGAAGTCGCAATCGCCTTCGAAATGGCTG TGCCAACAGGCTTGGGGCTTGCAGCAGGATGCCTTCTTCCAACCCCGCTAGTGGGTCAGGAGGGGAAGTG GTCATGTGGGCTCGAAAAAACCCAGTTGTGCTCTTCTTCCGAGAGGATCCACCAGACCCAGTTATGGCAG TCTACAAACAGCATAAATCTGAAACCAGGGACAGTAGCATCTCTACAAACAGCATAAATCTGAAACCAGG GACAGTAGCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200777 representing NM_181771
Red=Cloning site Green=Tags(s) MEEQARPPSRPAASATLPGSAHPGGAASTATAAALSFSSVATVTLGNQSDAGRPEAAGSRGPAPLLWHGA AVAAQALVLLLIFLLSSLGNCAVMGVIVKHRQLRTVTNAFILSLSLSDLLTALLCLPAAFLDLFAPPGDS GPWRSFCAASRFFSSCFGIVSTFSVALISLDRYCAIVRPPRDKLGRRRALQLLAGAWLAALGFSLPWELL RAPREPPTPQSFHRCLYRTSPDPAQLGAAYSVGLVVACYLLPFLLMCFCRYHICKTVRLSDVRVRPMTTY ARVLRFFSEVRTATTVLIMIVFVICCWGPYCFLVLLAATRQGQTTQAPSLLNVAAVWLTWANGAINPVIY AIRNPNISMFLGRNREEGYRTRNMDVFLPSQGLGFQARSRNRLRNGCANRLGACSRMPSSNPASGSGGEV VMWARKNPVVLFFREDPPDPVMAVYKQHKSETRDSSISTNSINLKPGTVA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181771 |
ORF Size | 1371 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 1374 bp |
RefSeq ORF | 1374 bp |
Locus ID | 314213 |
UniProt ID | Q7TQN7 |
MW | 49.6 kDa |
Gene Summary | G protein-coupled receptor of the family of rhodopsin-type GPCRs [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN200777 | Gpr135 (untagged ORF) - Rat G protein-coupled receptor 135 (Gpr135), (10 ug) |
CNY 5,990.00 |
|
RR200777L3 | Lenti ORF clone of Gpr135 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 135 (Gpr135), (10 ug) |
CNY 7,790.00 |
|
RR200777L4 | Lenti ORF clone of Gpr135 (mGFP-tagged ORF) - Rat G protein-coupled receptor 135 (Gpr135), (10 ug) |
CNY 8,550.00 |