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Mpp5 (NM_001108034) Rat Tagged ORF Clone
CAT＃: RR200931
- TrueORF®
Mpp5 (Myc-DDK-tagged ORF) - Rat membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (Mpp5), (10 ug)
"NM_001108034" in other vectors (3)
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CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR200931 representing NM_001108034
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACAACATCGTATATGAATGGGCATGTGACAGAGGAATCAGACAGCGGAATAAAAAATCTTGGTCTTG CATCACCAGAGGAACATCCGAAACACAGAGAGATGGCTGTTGACTGCCCTGGAGATTTGGGCACCAGATT GATGCCAGTGCGAAGAAGTGCACAGTTAGAGCGCATTCGACAGCAACAGGAGGATATGAGACGTAGGCGT GAAGAAGAAGGGAAAAAGCAAGAACTTGACCTAAATTCTTCCATGAGACTTAAGAAACTAGCTCAAATTC CTCCGAAGACTGGAATAGATAACCCTATATTTGACACAGAAGAAGGAATTGTTTTAGAAAGTCCTCACTA TGCTGTGAAAATATTAGAAGTTGAAGACTTGTTCTCTTCACTTAAACATATCCAGCACACTTTGGTTGAT TCTCAGAGCCAGGAGGATATTTCACTGCTTTTACAGCTTGTACAAAATAGAGATTTTCAGAATGCATTCA AGATACACAATGCTGTCACAGTGCACATGAACAAGGCCAGCCCTCCGTTTCCTCTTATCGCCAATGTACA AGACCTTGTACAAGAGGTACAAACTGTCTTAAAGCCAGTCCATCAGAAGGAAGGACAAGAATTAACTGCT TTGTTAAATGCTCCACATATTCAGGCACTTTTACTGGCCCATGATAAGGTTGCTGAGCAGGAAATGCAGC TAGAGCCCATAACAGATGAGAGAGTCTATGAAAGTATCGGCCATTATGGGGGAGAAACTGTAAAAATAGT TCGTATAGAAAAGGCTCGGGATATTCCATTGGGTGCCACGGTTCGTAATGAGATGGATTCTGTCATCATA AGTCGGATAGTGAAAGGAGGCGCGGCAGAGAAGAGCGGGCTTCTGCACGAAGGAGACGAAGTCCTAGAGA TCAATGGCATCGAAATCCGGGGCAAGGACGTCAATGAGGTTTTTGACTTGTTGTCTGATATGCACGGTAC GTTGACATTTGTTCTGATTCCTAGTCAACAGATCAAGCCTCCTCCCGCCAAAGAAACTGTAATCCACGTG AAAGCTCATTTTGACTATGACCCCTCAGATGACCCTTATGTTCCCTGTCGAGAGTTAGGACTGTCTTTTC AAAAAGGAGATATACTTCATGTGATTAGTCAAGAAGATCCGAATTGGTGGCAGGCCTATAGAGAAGGAGA CGAAGACAATCAGCCTCTAGCTGGACTTGTTCCAGGGAAAAGTTTTCAGCAGCAAAGGGAAGCCATGAAG CAAACTATAGAAGAAGATAAGGAGCCAGAAAAATCAGGAAAACTATGGTGTGCAAAGAAGAATAAAAAGA AGAGGAAAAAGGTTTTATATAATGCCAATAAAAATGATGATTATGACAATGAAGAGATCCTAACGTATGA AGAAATGTCACTTTACCATCAGCCAGCAAATAGGAAAAGACCTATCATCTTGATTGGTCCCCAGAACTGT GGCCAGAATGAACTGCGCCAGAGACTCATGAACAAAGAAAAAGACCGCTTTGCATCTGCAGTTCCTCATA CAACTCGGAATAGGCGAGACCACGAAGTAGCTGGGAGAGATTACCACTTTGTTTCACGGCAAGCATTTGA AGCAGATATAGCAGCTGGCAAGTTCATTGAACATGGTGAATTTGAGAAAAACCTGTATGGAACCAGCATA GATTCTGTACGACAAGTAATCAACTCTGGCAAAATATGTCTTTTAAGTCTTCGAGCACAGTCCTTGAAGA CCCTCCGGAATTCAGATTTGAAACCATACATCATCTTCATTGCACCACCTTCACAAGAGAGACTTCGGGC GTTGTTAGCCAAGGAAGGCAAGAACCCAAAGCCGGAAGAATTAAGGGAGATTATTGAGAAGACGAGAGAG ATGGAACAGAACAACGGCCACTACTTCGACACTGCGATTGTGAATTCAGATCTTGATAAAGCCTATCAGG AACTGCTTAGGCTTATTAACAAACTGGACACCGAGCCTCAGTGGGTGCCATCCACCTGGCTAAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR200931 representing NM_001108034
Red=Cloning site Green=Tags(s) MTTSYMNGHVTEESDSGIKNLGLASPEEHPKHREMAVDCPGDLGTRLMPVRRSAQLERIRQQQEDMRRRR EEEGKKQELDLNSSMRLKKLAQIPPKTGIDNPIFDTEEGIVLESPHYAVKILEVEDLFSSLKHIQHTLVD SQSQEDISLLLQLVQNRDFQNAFKIHNAVTVHMNKASPPFPLIANVQDLVQEVQTVLKPVHQKEGQELTA LLNAPHIQALLLAHDKVAEQEMQLEPITDERVYESIGHYGGETVKIVRIEKARDIPLGATVRNEMDSVII SRIVKGGAAEKSGLLHEGDEVLEINGIEIRGKDVNEVFDLLSDMHGTLTFVLIPSQQIKPPPAKETVIHV KAHFDYDPSDDPYVPCRELGLSFQKGDILHVISQEDPNWWQAYREGDEDNQPLAGLVPGKSFQQQREAMK QTIEEDKEPEKSGKLWCAKKNKKKRKKVLYNANKNDDYDNEEILTYEEMSLYHQPANRKRPIILIGPQNC GQNELRQRLMNKEKDRFASAVPHTTRNRRDHEVAGRDYHFVSRQAFEADIAAGKFIEHGEFEKNLYGTSI DSVRQVINSGKICLLSLRAQSLKTLRNSDLKPYIIFIAPPSQERLRALLAKEGKNPKPEELREIIEKTRE MEQNNGHYFDTAIVNSDLDKAYQELLRLINKLDTEPQWVPSTWLR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001108034 |
| ORF Size | 2025 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001108034.1, NP_001101504.1 |
| RefSeq Size | 2703 bp |
| RefSeq ORF | 2028 bp |
| Locus ID | 314259 |
| UniProt ID | B4F7E7 |
| MW | 77.2 kDa |
| Gene Summary | Plays a role in tight junction biogenesis and in the establishment of cell polarity in epithelial cells (By similarity). Also involved in adherens junction biogenesis by ensuring correct localization of the exocyst complex protein EXOC4/SEC8 which allows trafficking of adherens junction structural component CDH1 to the cell surface (By similarity). Plays a role through its interaction with CDH5 in vascular lumen formation and endothelial membrane polarity (By similarity). Required during embryonic and postnatal retinal development (By similarity). Required for the maintenance of cerebellar progenitor cells in an undifferentiated proliferative state, preventing premature differentiation, and is required for cerebellar histogenesis, fissure formation and cerebellar layer organization (By similarity). Plays a role in the radial and longitudinal extension of the myelin sheath in Schwann cells (By similarity). May modulate SC6A1/GAT1-mediated GABA uptake by stabilizing the transporter (By similarity). May play a role in the T-cell receptor-mediated activation of NF-kappa-B (By similarity). Required for localization of EZR to the apical membrane of parietal cells and may play a role in the dynamic remodeling of the apical cytoskeleton (By similarity). Required for the normal polarized localization of the vesicular marker STX4 (By similarity). Required for the correct trafficking of the myelin proteins PMP22 and MAG (PubMed:20237282).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN200931 | Mpp5 (untagged ORF) - Rat membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (Mpp5), (10 ug) |
CNY 8,740.00 |
|
| RR200931L3 | Lenti ORF clone of Mpp5 (Myc-DDK-tagged ORF) - Rat membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (Mpp5), (10 ug) |
CNY 10,450.00 |
|
| RR200931L4 | Lenti ORF clone of Mpp5 (mGFP-tagged ORF) - Rat membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) (Mpp5), (10 ug) |
CNY 11,590.00 |
