Ptprf (NM_019249) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	LARFN5C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201268 representing NM_019249 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCGAGCCAGCGCCAGGGAGGAGGATGGTGCCCCTTGTGCCTGCCCTCGTGATGCTGGGTTTGA TGGCAGGTGCTCATGGCGACAGCAAACCTGTCTTTGTTAAGGTCCCCGAGGACCAGATCGGGCTGTCGGG AGGGGTGGCCTCCTTCGTGTGCCAAGCCACAGGGGAACCCAAGCCTCGAATCACATGGATGAAGAAGGGA AAGAAAGTCAGCTCCCAGCGCTTTGAGGTAATTGAGTTTGACGACGGAGCAGGGTCAGTGCTGCGGATCC AGCCATTACGAGTACAGCGAGACGAAGCCATCTATGAGTGCACGGCCACGAACAGTCTCGGCGAGATCAA CACAAGTGCCAAGCTGTCAGTACTTGAAGAGGACCAGCTGCCGTCTGGGTTCCCGACTATCGACATGGGA CCTCAACTGAAGGTGGTGGAGAAGGCTCGCACTGCCACCATGCTGTGTGCAGCTGGTGGAAATCCAGACC CTGAGATCTCTTGGTTCAAAGACTTCCTTCCTGTGGACCCTGCTTCAAGCAACGGTCGTATCAAGCAGCT GCGTTCAGGTGCATTGCAGATTGAGAGCAGCGAGGAGTCTGACCAAGGCAAGTACGAGTGTGTGGCAACC AACTCGGCAGGCACACGCTACTCGGCCCCCGCCAACCTGTATGTGCGAGTGCGCCGCGTGGCTCCTCGTT TCTCCATCCCTCCCAGCAGCCAAGAGGTAATGCCGGGCGGCAACGTGAACCTCACGTGTGTGGCAGTGGG AGCACCCATGCCGTATGTGAAGTGGATGATGGGCGCCGAGGAACTTACCAAAGAGGACGAGATGCCAGTT GGCCGCAATGTGCTGGAGCTCAGCAATGTCATGCGGTCTGCCAACTATACCTGTGTGGCCATCTCTTCAC TGGGCATGATAGAGGCCACAGCCCAGGTCACAGTAAAAGCTCTGCCAAAGCCTCCAATTGATCTTGTGGT GACAGAGACAACTGCCACCAGTGTTACCCTGACATGGGACTCTGGAAATACCGAGCCTGTGTCTTTCTAC GGCATCCAGTACCGTGCAGCTGGCACAGACGGCCCCTTCCAGGAAGTGGATGGTGTGGCCAGCACCCGAT ACAGCATTGGTGGCCTCAGCCCTTTTTCAGAATATGCCTTCCGTGTGTTGGCTGTTAACAGCATTGGGCG AGGACCACCCAGTGAGGCAGTGCGAGCGCGAACGGGTGAGCAGGCACCCTCCAGCCCTCCACGCCGTGTG CAGGCCCGAATGCTGAGCGCCAGCACCATGCTGGTACAGTGGGAACCCCCTGAAGAGCCCAATGGCCTGG TACGGGGATATCGAGTCTACTACACCCCAGACTCTCGACGCCCCTTGAGCGCCTGGCACAAGCATAACAC CGACGCAGGACTCCTTACCACCGTGGGCAGCCTGCTACCTGGCATCACCTACAGCCTCCGCGTTCTGGCC TTTACTGCCGTGGGCGACGGCCCTCCCAGCCCCACCATCCAAGTCAAGACACAGCAGGGAGTGCCTGCAC AGCCTGCAGACTTCCAGGCCAAAGCCGAGTCGGACACCAGGATCCAGCTATCATGGCTGCTGCCCCCGCA GGAGCGGATCATCAAGTATGAGCTGGTGTACTGGGCAGCAGAGGATGAAGGCCAGCAGCACAAGGTGACC TTCGACCCCACCTCCTCCTACACTCTGGAGGACCTGAAGCCTGACACACTGTACCACTTCCAGCTGGCTG CTCGCTCAGATTTGGGGGTGGGCGTCTTCACTCCCACCGTAGAGGCCTGTACAGCACAGTCCACCCCATC CGCCCCTCCCCAGAAGGTGACATGTGTGAGCACGGGCTCCACCACGGTCCGGGTAAGTTGGGTTCCACCG CCGGCTGACAGCCGCAACGGCATTATCACCCAGTACTCCGTGGCCTATGAGGCAGTGGACGGCGAGGACC GTAAACGGCATGTGGTGGATGGCATCAGCCGTGAGCATTCCAGCTGGGACCTGTTGGGCCTGGAGAAGTG GACGGAGTACCGGGTGTGGGTGCGGGCACACACAGATGTGGGCCCTGGCCCTGAGAGCAGCCCGGTACTG GTGCGCACCGACGAGGACGTGCCTAGCGGGCCACCACGGAAGGTGGAGGTTGAGCCTCTGAACTCCACTG CTGTGCATGTCTCCTGGAAGCTGCCCGTCCCCAACAAGCAGCACGGACAGATTCGTGGCTACCAGGTCAC CTATGTGCGGCTGGAGAATGGGGAGCCCCGAGGCCAGCCCATCATCCAGGATGTCATGCTGGCTGAGGCT CAGGAAACCACCATCAGCGGCCTCACCCCCGAGACCACCTACTCCATTACTGTTGCTGCCTACACCACTA AGGGGGATGGTGCCCGAAGCAAGCCCAAAGTGGTTACTACCACAGGGGCAGTCCCAGGACGGCCCACCAT GATGGTCAGCACCACGGCCATGCACACCGCGCTGCTGCAGTGGCACCCACCCAAGGAGCTGCCTGGAGAG CTGCTGGGCTACCGGCTACAATACCGTAGGGCCGACGAGGCGAGGCCCAACACCATAGATTTTGGCAAGG ATGACCAGCATTTTACAGTCACCGGTCTGCACAAAGGGGCCACCTACATCTTCCGGCTGGCTGCCAAGAA CCGGGCTGGCCCAGGTGAAGAGTTTGAGAAAGAGATCACGACCCCCGAGGATGCGCCCAGTGGCTTTCCT CAAAACCTCCGAGTGACAGGACTGACCACGTCTACCACGGAATTGGCCTGGGACCCGCCGGTGCTGGCAG AGAGGAATGGTCGCATCACCAACTATACCGTGGTCTACCGTGACATCAACAGCCAGCATGAACTCCAGAA TGTCACTGGTGATGTACACCTTACACTCTTGGGCCTCAAACCGGACACCACTTATGACATCAAGGTCCGT GCACATACCAGCAAAGGCGCCGGCCCACTCAGCCCCAGCATCCAGTCCCGGACCATGCCCATGGAGCAAG TGTTTGCCAAGAATTTCCGTGTGGCCGCTGCAATGAAGACATCTGTGCTGCTCAGTTGGGAGGTCCCCGA CTCTTACAAGTCAGCTGTACCCTTCAAGATCCTGTACAATGGGCAGAGCGTGGAGGTGGATGGGCACTCC ATGCGGAAGCTGATTGCAGACCTGCAACCCAACACAGAGTACTCCTTCGTCCTGATGAACCGCGGCACGA GCGCTGGGGGCCTACAGCACCTGGTGTCCATCCGCACTGCCCCAGACCTCCTGCCTCAGAAGCCACTGCC TGCCTCTGCCTTTATAGAGGATGGCCGCTTCTCCCTCTCCATGCCTCAAGTGCAGGACCCCTCGCTAGTC AGGTGGTTCTACATTGTGGTGGTGCCCATTGACCGTGTGGGCGGGAACCTGCTGGCACCAAGATGGAGCA CACCAGAGGAGCTGGAGCTGGACGAGCTTCTGGAGGCCATCGAGCAGGGTGAGGAGAAACAGCGGAGGCG TCGGCGCCAAGCAGAGCGGCTGAAGCCTTACGTGGCAGCTCAAGTGGACGAGCTCCCTGAGACCTTCACC CTGGGGGACAAGAAGAACTACCGTGGCTTCTACAACCGGCCCCTGTCTCCGGATCTGAGTTACCAGTGCT TTGTGCTCGCCTCCCTCAAGGAACCCATGGACCAGAAGCGCTATGCCTCCAGCCCCTACTCGGACGAGAT CGTAGTCCAGGTGACACCAGCCCAGCAGCAGGAGGAGCCCGAGATGCTGTGGGTGACAGGCCCTGTCCTG GCAGTCATTCTCATCATACTCATTGTCATCGCTATCCTCCTATTCAAGAGGAAGAGAACACATTCCCCAT CGTCAAAGGATGAGCAGTCAATCGGGCTGAAGGACTCCCTGTTGGCCCACTCTTCTGACCCTGTGGAAAT GCGAAGGCTTAACTACCAGACCCCAGGTATGCGAGACCACCCGCCCATCCCCATCACTGACCTGGCAGAC AATATTGAACGTCTCAAAGCCAATGATGGGCTCAAGTTCTCCCAGGAGTATGAGTCCATTGACCCTGGAC AGCAGTTCACGTGGGAGAATTCCAACTCAGAGGTGAACAAGCCCAAGAACCGCTATGCAAACGTCATTGC CTATGACCACTCTCGAGTCCTCCTCACCTCCATTGATGGTGTTCCTGGGAGTGACTACATCAATGCCAAC TACATTGATGGCTACCGAAAGCAGAATGCCTACATCGCCACACAGGGTCCGCTGCCTGAGACCATGGGTG ATTTCTGGAGGATGGTGTGGGAACAGCGTATAGCCACAGTGGTCATGATGACCAGGCTAGAGGAGAAATC CCGGGTGAAGTGTGATCAGTATTGGCCAGCCCGTGGCACTGAGACCTATGGCCTCATTCAGGTGACCCTG GTGGACACTGTGGAGTTGGCCACGTACACCATGCGCACCTTCGCACTCCATAAGAGCGGCTCCAGTGAGA AGCGTGAGCTGCGTCAGTTCCAGTTCATGGCCTGGCCAGACCATGGGGTTCCTGAGTACCCCACTCCCAT CTTGGCCTTCCTGAGACGGGTCAAGGCCTGTAACCCGCTAGATGCAGGACCCATGGTGGTGCATTGCAGT GCTGGTGTGGGCCGCACGGGCTGCTTCATTGTCATCGACGCAATGCTGGAGCGTATGAAGCACGAGAAGA CAGTTGACATCTATGGCCACGTGACGTGCATGCGCTCACAAAGGAACTACATGGTGCAGACAGAGGACCA GTATGTGTTCATCCACGAGGCACTGTTAGAGGCTGCCATGTGCGGACACACTGAGGTGCTTGCTCGAAAC CTCTATGCCCACATCCAGAAGCTAGGCCAAGTGCCTCCCGGGGAGAGTGTCACTGCCATGGAGCTTGAGT TCAAGTTGCTGGCCGGCTCCAAGGCCCGCGCGTCTCGCTTCATTAGTGCCAACCTGCCCTGCAACAAGTT CAAGAACCGCCTGGTGAACATCATGCCCTATGAGCTGACTCGAGTGTGCCTGCAACCCATCCGTGGTGTG GAGGGCTCTGACTACATCAATGCCAGCTTTCTAGATGGCTACAGACAGCAGAAGGCCTACATAGCTACAC AGGGGCCTCTGGCGGAGAGCACCGAGGACTTCTGGCGCATGTTATGGGAGCACAATTCCACCATCATCGT CATGCTGACGAAGCTTCGGGAGATGGGCAGGGAGAAATGTCACCAGTACTGGCCAGCAGAGCGCTCTGCT CGCTATCAATACTTTGTCGTTGACCCGATGGCTGAGTACAACATGCCCCAGTATATCCTGCGTGAATTCA AAGTCACAGATGCCCGGGATGGGCAGTCAAGGACAATCCGACAGTTCCAGTTTACAGACTGGCCAGAGCA AGGAGTACCCAAGACGGGGGAAGGCTTCATCGACTTCATCGGGCAGGTGCACAAGACAAAGGAGCAGTTT GGACAGGATGGGCCCATCACAGTACACTGCAGTGCTGGTGTGGGCCGCACAGGGGTGTTTATCACCCTGA GCATTGTCCTGGAGCGCATGCGGTACGAGGGCGTGGTTGACATGTTCCAGACCGTGAAGACCCTCCGCAC ACAGCGCCCTGCAATGGTGCAGACAGAGGACCAATACCAGCTGTGCTACCGTGCGGCCCTGGAATACCTC GGCAGCTTTGATCACTATGCAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201268 representing NM_019249 Red=Cloning site Green=Tags(s) MAPEPAPGRRMVPLVPALVMLGLMAGAHGDSKPVFVKVPEDQIGLSGGVASFVCQATGEPKPRITWMKKG KKVSSQRFEVIEFDDGAGSVLRIQPLRVQRDEAIYECTATNSLGEINTSAKLSVLEEDQLPSGFPTIDMG PQLKVVEKARTATMLCAAGGNPDPEISWFKDFLPVDPASSNGRIKQLRSGALQIESSEESDQGKYECVAT NSAGTRYSAPANLYVRVRRVAPRFSIPPSSQEVMPGGNVNLTCVAVGAPMPYVKWMMGAEELTKEDEMPV GRNVLELSNVMRSANYTCVAISSLGMIEATAQVTVKALPKPPIDLVVTETTATSVTLTWDSGNTEPVSFY GIQYRAAGTDGPFQEVDGVASTRYSIGGLSPFSEYAFRVLAVNSIGRGPPSEAVRARTGEQAPSSPPRRV QARMLSASTMLVQWEPPEEPNGLVRGYRVYYTPDSRRPLSAWHKHNTDAGLLTTVGSLLPGITYSLRVLA FTAVGDGPPSPTIQVKTQQGVPAQPADFQAKAESDTRIQLSWLLPPQERIIKYELVYWAAEDEGQQHKVT FDPTSSYTLEDLKPDTLYHFQLAARSDLGVGVFTPTVEACTAQSTPSAPPQKVTCVSTGSTTVRVSWVPP PADSRNGIITQYSVAYEAVDGEDRKRHVVDGISREHSSWDLLGLEKWTEYRVWVRAHTDVGPGPESSPVL VRTDEDVPSGPPRKVEVEPLNSTAVHVSWKLPVPNKQHGQIRGYQVTYVRLENGEPRGQPIIQDVMLAEA QETTISGLTPETTYSITVAAYTTKGDGARSKPKVVTTTGAVPGRPTMMVSTTAMHTALLQWHPPKELPGE LLGYRLQYRRADEARPNTIDFGKDDQHFTVTGLHKGATYIFRLAAKNRAGPGEEFEKEITTPEDAPSGFP QNLRVTGLTTSTTELAWDPPVLAERNGRITNYTVVYRDINSQHELQNVTGDVHLTLLGLKPDTTYDIKVR AHTSKGAGPLSPSIQSRTMPMEQVFAKNFRVAAAMKTSVLLSWEVPDSYKSAVPFKILYNGQSVEVDGHS MRKLIADLQPNTEYSFVLMNRGTSAGGLQHLVSIRTAPDLLPQKPLPASAFIEDGRFSLSMPQVQDPSLV RWFYIVVVPIDRVGGNLLAPRWSTPEELELDELLEAIEQGEEKQRRRRRQAERLKPYVAAQVDELPETFT LGDKKNYRGFYNRPLSPDLSYQCFVLASLKEPMDQKRYASSPYSDEIVVQVTPAQQQEEPEMLWVTGPVL AVILIILIVIAILLFKRKRTHSPSSKDEQSIGLKDSLLAHSSDPVEMRRLNYQTPGMRDHPPIPITDLAD NIERLKANDGLKFSQEYESIDPGQQFTWENSNSEVNKPKNRYANVIAYDHSRVLLTSIDGVPGSDYINAN YIDGYRKQNAYIATQGPLPETMGDFWRMVWEQRIATVVMMTRLEEKSRVKCDQYWPARGTETYGLIQVTL VDTVELATYTMRTFALHKSGSSEKRELRQFQFMAWPDHGVPEYPTPILAFLRRVKACNPLDAGPMVVHCS AGVGRTGCFIVIDAMLERMKHEKTVDIYGHVTCMRSQRNYMVQTEDQYVFIHEALLEAAMCGHTEVLARN LYAHIQKLGQVPPGESVTAMELEFKLLAGSKARASRFISANLPCNKFKNRLVNIMPYELTRVCLQPIRGV EGSDYINASFLDGYRQQKAYIATQGPLAESTEDFWRMLWEHNSTIIVMLTKLREMGREKCHQYWPAERSA RYQYFVVDPMAEYNMPQYILREFKVTDARDGQSRTIRQFQFTDWPEQGVPKTGEGFIDFIGQVHKTKEQF GQDGPITVHCSAGVGRTGVFITLSIVLERMRYEGVVDMFQTVKTLRTQRPAMVQTEDQYQLCYRAALEYL GSFDHYAT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_019249
ORF Size	5694 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_019249.1, NP_062122.1
RefSeq Size	6545 bp
RefSeq ORF	5697 bp
Locus ID	360406
UniProt ID	Q64604
MW	211.5 kDa
Gene Summary	11 kDa ectodomain isoform of the LAR protein tyrosine phosphatase receptor family; may play a role in homophilic cell adhesion and neurite outgrowth [RGD, Feb 2006]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.