Dnai1 (NM_001024342) Rat Tagged ORF Clone
CAT#: RR201269
- TrueORF®
Dnai1 (Myc-DDK-tagged ORF) - Rat dynein, axonemal, intermediate chain 1 (Dnai1), (10 ug)
"NM_001024342" in other vectors (3)
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CNY 9,030.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Dnaic1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR201269 representing NM_001024342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGAAACAGATTCGTAAGCAGAGTGTCATTTTGACCAGAGGAGCCAGGAAGCGAGAGGAAGACT CAGGGACTGATGTGGGAGAAGGAGCCGATGACTGGGCCCAATCCAAAGCCGCTGTGCGGCCCCCTGACCA GCTGGAGCTGACTGACGCGGAGTTAAAGGAGGAGTTCACTCGGATCCTGACAGCAAACAACCCACATGCA CCCCAGAATATTGTCAGGTACAGCTTCAAAGAACGCACATACAAGCTTATTGGTTTTGTGAACCAAATGG CAATTCACTTCAGTCAGGTGGGGAACCTGATACCCAAAGACTCAGATGAAGGCCGTCGACAGCACTACCG TGATGAGCTGGTGGCAGGTTCACAGGAGTCAGTCAAGGTGGTGACTTCAGACACAGAAATCCTTGAAGAA GAGGAAGAACCCAAAGAAGGAGAAGGAGAAGGAGAAGGAGAAGCAGAAGGAGAAGCAGAAGCTGGGAGCC AAACGGACGTACCTGCTGCAGCCGAGACGACTGAAAAGGTGATTGAAGAAGAACTGATGGCTCCTGTGCA GCCCAAGGAGCGAAAACTCACGAACCAGTTTAACTTCAGCGAGCGAGCATCTCAGACCTTCAACAACCCT CTCCGGGATCGAGAATGTCAGATGGAGCCTCCACCTCGGACAAACTTTTCAGCCACAGCCAATCAGTGGG AGATCTATGATGCCTATGTAGATGAGCTCGAGAAACTGGAAAAGACCAAAGAGAAGGAGAAGACCAAGAC TCCAGTGGCTAAGAAAACAGAGAAGATGGCCATGAGGAAACTAACATCTATGGAGTCTCAGAGTGATGAT ATCACCAAAGTGACCCAAGCTGCTAAAATCGTGGAGCGAATGGTCAACCAAAATACATACGATGATGTCG CTCAAGATTTTAAGTACTATGAGGATGCTGCTGATGAATACAGGGACCAGGAGGGTACGCTGCTGCCTCT CTGGAAGTTTCAAAATGACAAAGCCAAGCGCCTGGCCGTCACCGCCCTCTGCTGGAATCCAAAGTACAAG GATCTTTTTGCTGTGGGACATGGCTCCTATGACTTCATGAAACAGAGTCGGGGCATGCTGCTGCTCTACA GTATGAAGAACCCTAGTTTCCCTGAGTACATGTTCAGCAGTGAGAGCGGCATCATGTGTCTGGACATGCA TATGGACCACCCCTACCTGGTGGTCGTGGGCTACTATGACGGCAACGTGGCCATCTACAACCTCAAGAAG CCTCACTCACAGCCCTGCTTCCGCAGCACCTCCAAGTCTGGCAAGCACACGGACCCTGTATGGCAGGTCA AATGGCAGAAGGATGACATGGACCACAACCTTAACTTCTTTTCTGTGTCATCTGATGGCAGGATCGTGTC ATGGACCCTTGTAAAGAGCGAGCTGGTCCACATCGATGTTATCAAGCTGAAGGACGAAGGCAGCACTACA GAAATCCCTGAGGGGCTGCAGCTGCACACTGTAGGCTGTGGCACTGCCTTTGACTTCCACAAAGAGATTG ACTATCTGTTCCTCGTGGGCACAGAAGAGGGGAAAATCTACAAGTGCTCCAAATCCTACTCCAGCCAGTT CTTGGACACCTATGATGCTCACAACATGGCAGTAGACGCTGTGTTATGGAACCCATACCACGCCAGGGTC TTCATATCTTGCAGTTCTGACTGGACAGTGAAGATCTGGGACCACGCCATCAAGACCCCCATGTTCATCT ATGATCTGAACGCAGCTGTGGGTGACGTGGCCTGGGCACCGTACTCTTCCACTGTGTTTGCAGCAGTCAC CACGGATGGGAAGGCCCACGTGTTTGACTTGGCTGTCAACAAGTATGAAGCCATCTGCAACCAGCCTGTG GTGGCCAAGAAGAAGAACAAGATTACCCATGTGCAGTTCAACCCCATTCACCCCATCATAATAGTGGGCG ATGACCGTGGACACATCACCTGTCTCAAGCTCTCTCCCAACTTACGAAAGATGCCGAAGGAGAAGAAGGG GCAGGAAGTGCAGAAGGGTCCAGCTGTGGAGATCGCAAAATTGGACAAACTGCTAAACCTTGTGAGGGAA GTAAAAACCAAAACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR201269 representing NM_001024342
Red=Cloning site Green=Tags(s) MPSKQIRKQSVILTRGARKREEDSGTDVGEGADDWAQSKAAVRPPDQLELTDAELKEEFTRILTANNPHA PQNIVRYSFKERTYKLIGFVNQMAIHFSQVGNLIPKDSDEGRRQHYRDELVAGSQESVKVVTSDTEILEE EEEPKEGEGEGEGEAEGEAEAGSQTDVPAAAETTEKVIEEELMAPVQPKERKLTNQFNFSERASQTFNNP LRDRECQMEPPPRTNFSATANQWEIYDAYVDELEKLEKTKEKEKTKTPVAKKTEKMAMRKLTSMESQSDD ITKVTQAAKIVERMVNQNTYDDVAQDFKYYEDAADEYRDQEGTLLPLWKFQNDKAKRLAVTALCWNPKYK DLFAVGHGSYDFMKQSRGMLLLYSMKNPSFPEYMFSSESGIMCLDMHMDHPYLVVVGYYDGNVAIYNLKK PHSQPCFRSTSKSGKHTDPVWQVKWQKDDMDHNLNFFSVSSDGRIVSWTLVKSELVHIDVIKLKDEGSTT EIPEGLQLHTVGCGTAFDFHKEIDYLFLVGTEEGKIYKCSKSYSSQFLDTYDAHNMAVDAVLWNPYHARV FISCSSDWTVKIWDHAIKTPMFIYDLNAAVGDVAWAPYSSTVFAAVTTDGKAHVFDLAVNKYEAICNQPV VAKKKNKITHVQFNPIHPIIIVGDDRGHITCLKLSPNLRKMPKEKKGQEVQKGPAVEIAKLDKLLNLVRE VKTKT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001024342 |
ORF Size | 2115 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001024342.1, NP_001019513.1 |
RefSeq Size | 2441 bp |
RefSeq ORF | 2118 bp |
Locus ID | 500442 |
UniProt ID | Q5XIL8 |
MW | 80.1 kDa |
Gene Summary | Part of the dynein complex of respiratory cilia.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN201269 | Dnai1 (untagged ORF) - Rat dynein, axonemal, intermediate chain 1 (Dnai1), (10 ug) |
CNY 9,030.00 |
|
RR201269L3 | Lenti ORF clone of Dnai1 (Myc-DDK-tagged ORF) - Rat dynein, axonemal, intermediate chain 1 (Dnai1), (10 ug) |
CNY 10,830.00 |
|
RR201269L4 | Lenti ORF clone of Dnai1 (mGFP-tagged ORF) - Rat dynein, axonemal, intermediate chain 1 (Dnai1), (10 ug) |
CNY 11,970.00 |