Nsd1 (NM_001107337) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR201931 representing NM_001107337 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTCACCCGAAGAAAAAGTCTACACCATTGAAATATGAAGTTGGAGATCTCATTTGGGCAAAATTCA AGAGACGCCCATGGTGGCCCTGCAGGATTTGTTCTGATCCATTGATTAATACGCACTCAAAAATGAAAGT TGCCAATAGGAGGCCATATCGAGAATACTACGTGGAGGCTTTTGGAGATCCTTCTGAAAAAGCCTGGGTG GCTGGAAAAGCAATCGTCATGTTTGAAGGCAGACATCAATTTGAAGAACTACCTGTCCTTAGGAAAAGAG GGAAACAGAAAGAAAAAGGATATAGGCATAAGGTTCCTCAGAAAATCCTGAGTAAGTGGGAAGCCAGTGT TGGCCTTGCTGAGCAGTGTGATGTCCCCAGAGGACCTAAGACCCAGAAGTGTGTCCCCAGCTCGGCCAAG CTGGACAGCGAGGAAGACATGCCATTCGAGGACTGTGCAAACGATCCTGATTCAGAACATGACTTGTTGC TTAATGGCTGCTTGAAGTCTCTGGCTTTTGATTCTGAGCATTCTGCAGATGAAAAGGAAAAGCCCTGTGC CAAGTCTCGAGTTAGGAAAAGCTCTGATAATATAAAAAGGACTAGTGTGAAAAAGGGCCTCATGCCATTC GAAGCACAGAAAGAAGAACGGAGGGGGAAGTCTCCAGAGAACCTTGGCCTAGACTTTCTCTCTGGGGGTG TATCTGATAAACAGGCTTCTAATGAACTTTCCAGGATAGCAAACAGCCTCACAGGGTCTAGCGCCGCACC AGGACAGTTCCTCTTTTCCTCGTGTGGACAGAACACTGCTAAGACAGATTTTGAGACTCCAAATTGTGAT TCTTTATCAGGTTTGTCTGAAAGTGCTTTAATCTCTAAACATTCTGGAGAGAAAAAGAAACTCCAACCAG GCCAGGTCTGCAGCTCAAAAGTACAGCTCTGCTATGTTGGAGCTGGTGATGAGGAAAAGCGAAGTGATTC TGTCAGTGTGTGTACCACTTCTGATGATGGCAGCAGTGACCTGGATCCTACAGAACACAACTCAGAATTC CATAAAAGTGTCCTCGAAGTTACAGATGCGTTGGATAAAACAGAGAATGCTTTATCCATGCACAAAAATG AAACAAAGTATTCTAGGTATCCTGCCACAAACAGGGTAAAAGAGAAACAGAAATCCCTTATTACTAATTC ACATACAGATCACTTAATGGATTCTACCAAGACAGTGGAGCCTGGAACTGCTGAGATATCTCAGGTCAAT CTTTCTGATCTTAAAATCAGTAGTCCTATTCCCAAACCCCAACCAGAATTTAGAAATGATGGTCTCACTA CAAAATTCAATGCACCGCCAGGCATTCGTAATGAAAACTCACTGACAAAGGGTGGGCTTGCAAATCAAAC TCTGTTACCTTTGAAATGCCGACAGCCCAAGTTCCGGAGTATAAAATGCAAGCATAAAGAAAGCCCAACT GCTGCAGAAACCTCAGCTACGAGTGAGGACCTCAGTTTGAAATGCTGTTCTTCTGATACCAACGGCTCTC CTATGACCAGCATATCCAAAAGTGGGAAAGGAGAAGGACTAAAACTACTGAACAATATGCATGAGAAAAC CAGAGATTCGAGTGACATAGAAACAGCAGTGGTGAAGCATGTGCTGTCAGAATTGAAGGAGCTCTCTTAC AGATCATTAAGTGAGGATGTCAGTGATTCTGGAACATCCAAGTCATCAAAGCCGTTGCTGTTTTCTTCTG CTTCTAGTCAGAATCATATACCCATCGAACCAGATTACAAGTTTAGCACATTGCTAATGATGCTGAAAGA TATGCATGATAGTAAGACGAAGGAGCAAAGGTTGATGACTGCTCAAAATGTGGCTTCCTACCGCACTCCC GATCGCGGGGACTGTTCTTCTGGTAGTCCTGTAGGAACATCAAAGGTTTTGGTGTTAGGAGGCTCCACAC ACAATTCTGAAAAACCTGGAGATAGCACTCAGGACTCAGTTCGCTTGAGTCCTGGTGGGGGTGACTCTGC ATTGTCTGGAGAATTGTCTTCTTCCCTATCCGTCTTACCTTCTGACAAAAGAGATCTTCCTGCTTGTGGT AAAATTCGCTCTAACTGTATCCCAAGGCGCAACTGTGGCCGAGCAAAGCTATCACCCAAATTGAGAGTAA CAATTTCAACCCAGATGGCAAAACCTTCAGTAAACCCAAAAGCCTTAAAGACTGAGCGAAAGAGAAAGTT GAGCCGACTTCCAGCTGTGACACTTGCAGCTAACGGACTGGGAAACAAAGAAAGTGGAGGCTCAGTGAAT GGCCCATTGAAGGGTGGGGCTCAAGATCCTGCTAAAGAAGAGCCCCTTCAGCAAATGGACCTCTTAAGAA ATGAAGAGACCCATTTTGATAGCAAGGTTAAACAATCTGACCCTGATAAAATTCTTGAAAAAGAACCTTC TTTTGAGAACAGAAAAGGCCCAGAGGTGGGCTCTGAAATTAACATTGAGAATGATGAACCCCATGGTGTT GACCAAGTGGTCCCTAAAAAGCGGTGGCAGCGCTTAAACCAAAGGCGCCCTAAGCCTGGGAAACGTGCGA GTAGATTTAGGGAGAAAGAAAACTCTGAGGGTGCTTTTGGAGTCTTACTTCCTGGTGACCCTGTGCAGAA GGGCCGGGATGATTACTTAGAACAAAGAGCTCCTCCTACAAGCATACTCGAGGACTCAGCAGCAGATCCC AATCACGTTAGCCACTCAGAATCAGTTGGGCCTCGGCTGAATGTTTGTGATAAGTCCAGTGTCAGCATGG GCGACTTAGAAAAGGAGACGGGAATTCCCAGTTTGACGCCACAGACCAAGATCCCTGAGCCAGCTGTTCG GTCAGAGAAGAAACGCCTTAGGAAGCCGAGCAAGTGGCTTCTGGAATACACAGAAGAATATGATCAGATA TTTGCTCCTAAGAAAAAACAAAAGAAGGTACAGGAACAAGTGCATAAGGTAAGTTCCCGATGTGAAGACG AAAGCCTTCTTGCCCGATGTCGATCTAGTGCTCAGAACAAGCAGGTGGATGAGAATTCCTTGATTTCAAC TAAAGAAGAGCCTCCAGTTCTTGAAAGAGAGGCTCCATTTTTGGAAGGGCCATTGGTTCAGTCAGATCTT GGAGTTGCACATGCTGAGTTGCCACAGCTGACCTTGTCTGTTCCTGTGGCTCCAGAAGTTTCTCCGAGGC CTACCCTTGAGTCTGAGGAATTGCTTGTTAAGACACCAGGAAATTATGAAGGTAAGCGTCAAAGAAAACC AACTAAGAAACTTCTTGAATCCAATGATTTAGACCCTGGATTTATGCCAAAGAAGGGCGATCTTGGCCTT TCTAGAAAGTGCTGTGAGTCTGGTCACTTGGAGAATGGCGTTGGCGACTCACGTGCTACACCTCATTTGA AGGAGTTTGGTGGAGGCACTACCAGGATATTTGATAAGCCAAGGAAACGAAAACGGCAAAGGCATGGCAC AGCCAGAGTGCACTACAAAAGAGTGAAGAAGGAGGACTCAGCAAGAGAGACGCCCAGCTCAGCAGAGGGA GAACTGATGATTCACAGGACTGCTGCCAGCCCCAAGGAGATTCTCGAAGAGGGCATAGAACATGATCCTG GGATGTCTGCATCCAAAAGACTGCAGGGTGAACGAGGCGGAGGAGCAGCACTCAAGGAGAACGTCTGTCA GAACTGTGAGAAACTGGGTGAGCTGTTGTTGTGTGAGGCTCAGTGCTGTGGGGCTTTCCACCTGGAGTGC CTTGGCCTGACCGAGATGCCTCGAGGAAAATTCATCTGCAACGAGTGTCGCACAGGGATCCACACCTGTT TTGTATGTAAGCAGAGTGGAGAGGATGTGAAAAGGTGCCTTCTGCCTTTATGTGGCAAGTTTTACCATGA AGAGTGTGTTCAGAAGTACCCACCAACTGTCGTACAGAACAAGGGCTTCAGGTGCCCCCTCCACATCTGT ATAACTTGCCATGCTGCTAATCCAGCCAATGTGTCTGCTTCTAAAGGTCGTCTGATGCGCTGTGTCCGCT GTCCTGTGGCATACCATGCTAATGACTTTTGTCTTGCTGCTGGGTCAAAAATCCTCGCATCTAATAGTAT CATCTGCCCTAATCACTTCACCCCTAGACGGGGCTGCCGAAATCATGAGCATGTTAATGTCAGCTGGTGT TTTGTATGCTCAGAAGGAGGCAGCCTTCTGTGCTGTGATTCTTGTCCTGCTGCTTTTCATCGTGAATGCC TAAATATAGATATCCCCGAAGGAAACTGGTACTGCAATGACTGTAAGGCAGGCAAAAAGCCACACTACAG GGAGATTGTCTGGGTCAAAGTTGGCCGCTACAGGTGGTGGCCCGCTGAGATCTGCCATCCTCGAGCTGTT CCCTCTAACATTGACAAAATGAGGCATGACGTGGGAGAATTCCCTGTTCTCTTCTTTGGGTCCAATGACT ATCTGTGGACTCATCAGGCTCGGGTCTTCCCTTACATGGAAGGGGATGTGAGCAGCAAGGATAAGATGGG GAAAGGAGTGGATGGGACATATAAGAAAGCTCTTCAGGAAGCTGCAGCAAGATTTGAAGAGTTAAAAGCT CAAAAAGAACTAAGACAGCTGCAGGAAGACCGGAAGAACGACAAGAAGCCACCTCCTTATAAACATATAA AGGTGAACCGGCCCATTGGCAGAGTACAGATCTTCACTGCAGACTTGTCAGAAATTCCCCGTTGCAACTG TAAAGCTACTGATGAGAACCCCTGTGGGATAGACTCAGAGTGCATCAACCGGATGCTGCTCTACGAGTGC CACCCCACAGTGTGTCCTGCTGGAGGACGCTGTCAGAATCAGTGCTTCAGCAAGCGCCAGTATCCAGATG TTGAAATTTTCCGCACATTACAAAGGGGCTGGGGTCTCCGGACAAAAACAGATATTAAAAAGGGTGAATT TGTGAATGAATATGTGGGTGAGCTAATAGATGAAGAAGAGTGCAGAGCTCGGATCCGTTACGCACAAGAA CACGATATCACTAATTTCTATATGCTCACGCTAGACAAAGATCGAATTATTGATGCTGGTCCTAAAGGAA ACTATGCTCGGTTCATGAATCACTGCTGCCAGCCTAACTGTGAGACACAGAAGTGGTCTGTGAATGGAGA TACCCGAGTTGGTCTTTTTGCTTTGAGTGACATTAAAGCAGGAACTGAACTTACCTTCAATTATAACCTA GAATGTCTTGGGAATGGAAAGACTGTCTGTAAATGTGGAGCCCCGAACTGCAGTGGCTTCTTGGGTGTGA GGCCAAAGAATCAACCCATTGTCACAGAAGAAAAGTCCAGGAAATTCAAGAGGAAGCCACATGGGAAGCG CAGAAGCCAGGGTGAAGTCACCAAGGAGAGAGAGGATGAATGTTTCAGCTGTGGAGATGGTGGTCAGCTC GTCTCCTGCAAGAAGCCAGGCTGCCCGAAAGTTTACCATGCAGACTGTCTCAATCTGACCAAACGACCAG CAGGGAAGTGGGAGTGTCCTTGGCACCAGTGTGACGTGTGTGGGAAGGAAGCAGCCTCCTTCTGTGAGAT GTGCCCCAGCTCCTTTTGCAAGCAGCATCGAGAAGGGATGCTTTTCATTTCCAAGCTCGATGGGCGCCTG TCTTGTACTGAGCATGATCCCTGTGGGCCCAACCCTCTGGAACCAGGGGAGATCCGTGAGTATGTGCCTC CCACAGCGACGCTCTCTCCAAGCCCTGGCACTCAGCAGACAGAGCAGTCATCAGAGATGGGTACTCAGGG GCCCAAGAAATCTGATCAACCACCTACTGATGCCACCCAGATGCTGTCACTCTCCAAAAAAGCCGTAACA GGGACTTGTCAGAGGCCACTGCTCCCTGAGAGGCCTCCTGAGAGAACTGACTCTAGTTCCCACCTTTTAG ATAGGATCAGAGACCTTGCTGGGTCAGGGACCAAATCCCAGTCCTTGGTATCCAGCCAGAGACCACAGGA CAGGCCCCCTGCTAAAGAAGGACCAAGACCTCAGCCACCTGACAGAGCCTCTCCAGTGACCAGGCCAAGC TCCTCCCCTTCAGTCAGCTCCCTGCCATTGGAAAGACCTCTGAGAATGACTGAACCAAGGCTGGATAAAT CCATAGGTGCTGCCAGCCCAAAGTCCCAAGCAGTGGAGAAACCTCCAGCCCCCACTGGCCTGAGACTTTC ATCACCAGACAGGCTGCTAAACACCAACAGCCCTAAACCCCAGATTTCTGACAGGCCCCCAGACAAGTCC CACGCCTCCTTGACCCAGAGACTTCCACCTCCTGAGAAAGTGCTGTCCGCTGTGGTCCAGTCTCTAGTAG CTAAAGAGAAAGCACTGAGGCCTGTGGACCAGAACACCCAGGCGAAGCACAGAGCTGCTGTGGTGATGGA TCTCATAGACCTAACGCCTCGCCAGAAGGAGCGGGCTGCTTCTCCTCAGGAAGTCACAGCGCAGGCTGAT GAGAAGACACCAGTGCTAGAGTCAAGCTCAAGGCCGACTAGCAGAGGTCTGGGGCATGTGCCCCGAGCTG TGGAGAAAGGCGGCGTGTCAGATCCCCTCCAGCCGCCTGGGAAGTCTGCAGCTCCTTCAGAACACCCTTG GCAAGCTGTTAAGTCGCTCACCCATGCCAGATTCCTTTCTCCGCCTTCTGCCAAGGCCTTTTTATATGAA TCAGCAATTCAGGCCTCAGGAAGAGCTCCTGTGGGGTCTGAGCAGACCCCCGGCCCTCCCAGCCCAGCAC CAGGCCTGCTCAAACAGGTGAAGCAGCTATCTAGAGGCCTCACTGCCAAGAGTGGGCAGTCCTTCAGGTC TCTTGGGAAGATCTCAGCTTCCCTCCCCAATGAAGAAAAGAAGTTGACAACCACAGAACAGAGTCCCTGG GGCCTGGGAAAAGCCTCACCGGGGGCAGGGCTTTGGCCAATAGTGGCTGGACAAACACTAGCCCAGGCTT GCTGGTCATCTGGGGGCACACAAACTTTGGCACAAAACTGCTGGTCCCTTGGAAGAGGGCAAGACCCCAA ACCAGAACAGAATGCCATCCAAGCTCTTAACCAGGCCCCTTCTAGTCGCAAATGTGCAGAGTCAGAACAG AAAAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR201931 representing NM_001107337 Red=Cloning site Green=Tags(s) MCHPKKKSTPLKYEVGDLIWAKFKRRPWWPCRICSDPLINTHSKMKVANRRPYREYYVEAFGDPSEKAWV AGKAIVMFEGRHQFEELPVLRKRGKQKEKGYRHKVPQKILSKWEASVGLAEQCDVPRGPKTQKCVPSSAK LDSEEDMPFEDCANDPDSEHDLLLNGCLKSLAFDSEHSADEKEKPCAKSRVRKSSDNIKRTSVKKGLMPF EAQKEERRGKSPENLGLDFLSGGVSDKQASNELSRIANSLTGSSAAPGQFLFSSCGQNTAKTDFETPNCD SLSGLSESALISKHSGEKKKLQPGQVCSSKVQLCYVGAGDEEKRSDSVSVCTTSDDGSSDLDPTEHNSEF HKSVLEVTDALDKTENALSMHKNETKYSRYPATNRVKEKQKSLITNSHTDHLMDSTKTVEPGTAEISQVN LSDLKISSPIPKPQPEFRNDGLTTKFNAPPGIRNENSLTKGGLANQTLLPLKCRQPKFRSIKCKHKESPT AAETSATSEDLSLKCCSSDTNGSPMTSISKSGKGEGLKLLNNMHEKTRDSSDIETAVVKHVLSELKELSY RSLSEDVSDSGTSKSSKPLLFSSASSQNHIPIEPDYKFSTLLMMLKDMHDSKTKEQRLMTAQNVASYRTP DRGDCSSGSPVGTSKVLVLGGSTHNSEKPGDSTQDSVRLSPGGGDSALSGELSSSLSVLPSDKRDLPACG KIRSNCIPRRNCGRAKLSPKLRVTISTQMAKPSVNPKALKTERKRKLSRLPAVTLAANGLGNKESGGSVN GPLKGGAQDPAKEEPLQQMDLLRNEETHFDSKVKQSDPDKILEKEPSFENRKGPEVGSEINIENDEPHGV DQVVPKKRWQRLNQRRPKPGKRASRFREKENSEGAFGVLLPGDPVQKGRDDYLEQRAPPTSILEDSAADP NHVSHSESVGPRLNVCDKSSVSMGDLEKETGIPSLTPQTKIPEPAVRSEKKRLRKPSKWLLEYTEEYDQI FAPKKKQKKVQEQVHKVSSRCEDESLLARCRSSAQNKQVDENSLISTKEEPPVLEREAPFLEGPLVQSDL GVAHAELPQLTLSVPVAPEVSPRPTLESEELLVKTPGNYEGKRQRKPTKKLLESNDLDPGFMPKKGDLGL SRKCCESGHLENGVGDSRATPHLKEFGGGTTRIFDKPRKRKRQRHGTARVHYKRVKKEDSARETPSSAEG ELMIHRTAASPKEILEEGIEHDPGMSASKRLQGERGGGAALKENVCQNCEKLGELLLCEAQCCGAFHLEC LGLTEMPRGKFICNECRTGIHTCFVCKQSGEDVKRCLLPLCGKFYHEECVQKYPPTVVQNKGFRCPLHIC ITCHAANPANVSASKGRLMRCVRCPVAYHANDFCLAAGSKILASNSIICPNHFTPRRGCRNHEHVNVSWC FVCSEGGSLLCCDSCPAAFHRECLNIDIPEGNWYCNDCKAGKKPHYREIVWVKVGRYRWWPAEICHPRAV PSNIDKMRHDVGEFPVLFFGSNDYLWTHQARVFPYMEGDVSSKDKMGKGVDGTYKKALQEAAARFEELKA QKELRQLQEDRKNDKKPPPYKHIKVNRPIGRVQIFTADLSEIPRCNCKATDENPCGIDSECINRMLLYEC HPTVCPAGGRCQNQCFSKRQYPDVEIFRTLQRGWGLRTKTDIKKGEFVNEYVGELIDEEECRARIRYAQE HDITNFYMLTLDKDRIIDAGPKGNYARFMNHCCQPNCETQKWSVNGDTRVGLFALSDIKAGTELTFNYNL ECLGNGKTVCKCGAPNCSGFLGVRPKNQPIVTEEKSRKFKRKPHGKRRSQGEVTKEREDECFSCGDGGQL VSCKKPGCPKVYHADCLNLTKRPAGKWECPWHQCDVCGKEAASFCEMCPSSFCKQHREGMLFISKLDGRL SCTEHDPCGPNPLEPGEIREYVPPTATLSPSPGTQQTEQSSEMGTQGPKKSDQPPTDATQMLSLSKKAVT GTCQRPLLPERPPERTDSSSHLLDRIRDLAGSGTKSQSLVSSQRPQDRPPAKEGPRPQPPDRASPVTRPS SSPSVSSLPLERPLRMTEPRLDKSIGAASPKSQAVEKPPAPTGLRLSSPDRLLNTNSPKPQISDRPPDKS HASLTQRLPPPEKVLSAVVQSLVAKEKALRPVDQNTQAKHRAAVVMDLIDLTPRQKERAASPQEVTAQAD EKTPVLESSSRPTSRGLGHVPRAVEKGGVSDPLQPPGKSAAPSEHPWQAVKSLTHARFLSPPSAKAFLYE SAIQASGRAPVGSEQTPGPPSPAPGLLKQVKQLSRGLTAKSGQSFRSLGKISASLPNEEKKLTTTEQSPW GLGKASPGAGLWPIVAGQTLAQACWSSGGTQTLAQNCWSLGRGQDPKPEQNAIQALNQAPSSRKCAESEQ KK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107337
ORF Size	7143 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107337.1, NP_001100807.1
RefSeq Size	9233 bp
RefSeq ORF	7146 bp
Locus ID	306764
MW	262.5 kDa

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