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Sardh (NM_053664) Rat Tagged ORF Clone
CAT＃: RR202318
- TrueORF®
Sardh (Myc-DDK-tagged ORF) - Rat sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_053664" in other vectors (3)
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CNY 11,880.00
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CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202318 representing NM_053664
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCACTGAGCCGGGTCCTCCGTGTAGCTGCCACCTGTCCTCGAGGGAGATCTGCTCGCAACCTGG GGCTACAGCCCCTAGCCAGGGAGGCCAGGCCCACCACTGAGAAGAGTGTTCCATACCAGTGCACCCTGAA GGAGGAGGCCCAAGGTGCCTCTGTGGTGCCCCAAGGACCAAGCCAGCCCCTGCCTAGCACAGCCAATGTT GTGGTCATTGGTGGGGGCAGTTTGGGCTGCCAGACTCTGTACCACTTGGCCAAGCTGGGTGTAGGTGGAG TTGTGCTGCTGGAGAGAGAGCGACTGACCTCTGGGACCACCTGGCACACAGCAGGCCTACTGTGGCAGCT GCGGCCCAGCGATGTGGAAGTAGAGCTTCTGGCTCACACACGGCAAGTGGTGAGCCATGATCTGGAAGAG GAGACTGGGCTTCACACAGGCTGGATCCAGAATGGTGGCCTCTTCATCGCCTCCAACCAGCAGCGCCTGG ATGAATACAAGAGGCTCATGTCGTTGGGCAAGGCCTACGGTATAGAATCCCATGTGCTGAGCCCTGCAGA GACCAAGGATCTGTATCCGCTGATGAATGTGGACGACCTCTATGGGACCCTGTATGTGCCTCGGGATGGT ACCATGGACCCGGCTGGCACCTGCACTACCCTCACCAGAGCAGCAGTGGCTAGGGGAGCTCAGGTCATTG AAAACTGTGCAGTGACTGGCATCCGTGTGCGGACAGACGACTTTGGAGTGCGGCGGGTGACAGCTGTGGA GACTCAACATGGCTCCATCCAGACACCCTGCGTGGTCAACTGTGCAGGGGTGTGGGCGAGCTCCGTGGGC CGGATGGCTGGAGTGAAGGTCCCGCTGGTGGCCATGCACCATGCCTACGTTGTCACTGAGCGCATTGAGG GGATCCAGAACATGCCCAATGTCCGGGATCATGATGCTTCTGTCTACCTCCGCCTGCAGGGGGATGCCTT GTCTGTGGGTGGCTATGAGGCCAACCCCATCTTTTGGGATGAGGTGTCAGACAAATTTGCCTTTGGCCTC TTTGACCTGGACTGGGATGTGTTCACTCAGCACATTGAAGGTGCCATTAACCGTGTCCCCGTGTTGGAGA AAACGGGGATCAAGTCCACTGTCTGTGGCCCTGAATCCTTCACACCCGACCATAAGCCCCTGATGGGGGA GGCTCCTGAGCTCCGAGGCTTCTTCCTTGGCTGTGGCTTCAACAGTGCAGGCATGATGTTGGGTGGTGGC TGTGGACAGGAGCTGGCCCATTGGATTGTGCATGGGCGCCCAGAGAAGGACATGTACAGCTATGACATCA GGCGTTTCCACCACTCGCTCACGGACCACCCACGCTGGATCCGAGAGCGCAGCCACGAGTCCTATGCCAA GAACTACTCGGTTGTCTTTCCTCACGATGAGCCCCTGGCGGGCCGCAACATGAGGAGGGACCCTCTGCAC GAGGAGCTGCTTGGACAAGGCTGTGTGTTTCAAGAACGACAGGGCTGGGAACGGCCAGGATGGTTCAACC CCCAAGAAACAGCTCAGGTCCTAGACTACGACTACTATGGGGCCTACGGACACCAGGCACACAAGGACTA CGCCTACAGCAGGCTGCTGGGCGATGAATATACCTTCGACTTCCCACCCCACCACTGCGTGATCCAGAAG GAATGTCTGGCCTGCAGAACGGCTGCCGCCGTGTTCAACATGTCTTACTTCGGGAAGTTCTACCTTCTGG GGGCAGACGCCAGGAAGGCTCCAGACTGGCTCTTCTCAGCAGATGTCAACCGACCCCCAGGCTCCACGGT GTACACATGCATGCTGAACCAACGGGGAGGCACCGAGAGCGACCTGACTGTTAGCTGCCTGGCCCCTGGT GCCCAGGCCAGTCCCCTGGCCCCTGCCTTTGAAGGGGATGGCTACTACCTAGCCGTAGGTGGGGCTGTGG CCCAACATAACTGGTCCCACATAAACACGGTGCTGCAGGATCAGGAGTTCCGGTGCCAGCTTATGGATTG TTCTGAAGATCTGGGCATGCTCAGTATCCAGGGCCCAGCCAGCCGTGACATTCTCCAGGATGTGCTTGAT GCTGACCTGAGCAATGAAGCCTTCCCCTTCTCCACCCACCAGCTGGTGAGAGCTGCTGGACACCTGGTCC GAGCCATCCGGCTATCCTTTGTCGGGGAGCTGGGATGGGAGCTGCATGTGCCCCAAGCGTCCTGCTTGCC TGTGTACCGGGCTGTGATGGCTGCAGGGGCGAAGCACGGCCTAGTCAATGCAGGCTACCGTGCCATCGAC TCCCTGAGCATTGAGAAAGGCTATCGGCACTGGCATGCAGACCTGAGGTCCGATGACAGCCCCCTGGAGG CAGGGTTGGCCTTCACCTGCAAGCTCAAGACCTCAGTGCCCTTCCTAGGCCGAGAGGCCCTGGAGAAGCA GCGTGCCACAGGCCTCCGACGACGCCTGGTGTGCCTCACTGTGGAAGAGGAGGTTCCTATGTTCGGCCTA GAGGCCATCTGGAGAAATGGCCAAGTGGTGGGCCATGTCCGGAGAGCTGACTTTGGATTCACCGTGAACA AGACCATCGCCTATGGCTACATCCGAGACCCCAGTGGAGGGCCAGTCTCTCTGGACTTTGTGAAGAATGG GGACTATGCCTTGGAGAGAATGGGGGTCACCTATGCTGCCCAGGTTCACCTGAAGTCTCCCTTTGATCCT GACAACAAGCGGGTGAAGGGAATCTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202318 representing NM_053664
Red=Cloning site Green=Tags(s) MASLSRVLRVAATCPRGRSARNLGLQPLAREARPTTEKSVPYQCTLKEEAQGASVVPQGPSQPLPSTANV VVIGGGSLGCQTLYHLAKLGVGGVVLLERERLTSGTTWHTAGLLWQLRPSDVEVELLAHTRQVVSHDLEE ETGLHTGWIQNGGLFIASNQQRLDEYKRLMSLGKAYGIESHVLSPAETKDLYPLMNVDDLYGTLYVPRDG TMDPAGTCTTLTRAAVARGAQVIENCAVTGIRVRTDDFGVRRVTAVETQHGSIQTPCVVNCAGVWASSVG RMAGVKVPLVAMHHAYVVTERIEGIQNMPNVRDHDASVYLRLQGDALSVGGYEANPIFWDEVSDKFAFGL FDLDWDVFTQHIEGAINRVPVLEKTGIKSTVCGPESFTPDHKPLMGEAPELRGFFLGCGFNSAGMMLGGG CGQELAHWIVHGRPEKDMYSYDIRRFHHSLTDHPRWIRERSHESYAKNYSVVFPHDEPLAGRNMRRDPLH EELLGQGCVFQERQGWERPGWFNPQETAQVLDYDYYGAYGHQAHKDYAYSRLLGDEYTFDFPPHHCVIQK ECLACRTAAAVFNMSYFGKFYLLGADARKAPDWLFSADVNRPPGSTVYTCMLNQRGGTESDLTVSCLAPG AQASPLAPAFEGDGYYLAVGGAVAQHNWSHINTVLQDQEFRCQLMDCSEDLGMLSIQGPASRDILQDVLD ADLSNEAFPFSTHQLVRAAGHLVRAIRLSFVGELGWELHVPQASCLPVYRAVMAAGAKHGLVNAGYRAID SLSIEKGYRHWHADLRSDDSPLEAGLAFTCKLKTSVPFLGREALEKQRATGLRRRLVCLTVEEEVPMFGL EAIWRNGQVVGHVRRADFGFTVNKTIAYGYIRDPSGGPVSLDFVKNGDYALERMGVTYAAQVHLKSPFDP DNKRVKGIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053664 |
| ORF Size | 2757 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053664.2, NP_446116.1 |
| RefSeq Size | 3069 bp |
| RefSeq ORF | 2760 bp |
| Locus ID | 114123 |
| UniProt ID | Q64380 |
| MW | 101.4 kDa |
| Gene Summary | a flavoenzyme involved in the oxidative degradation of choline to glycine [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202318 | Sardh (untagged ORF) - Rat sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 11,880.00 |
|
| RR202318L3 | Lenti ORF clone of Sardh (Myc-DDK-tagged ORF) - Rat sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 13,490.00 |
|
| RR202318L4 | Lenti ORF clone of Sardh (mGFP-tagged ORF) - Rat sarcosine dehydrogenase (Sardh), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 14,920.00 |
