Robo3 (NM_001108135) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202319 representing NM_001108135 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCACGCATCGTGGAGCAGCCGCCAGATCTGCTCGTTTCCAGGGGCGAGCCGGCCACTCTGCCCTGCC GTGCTGAAGGCCGGCCTCGACCCAACATCGAGTGGTACAAGAATGGGGCGCGCGTGGCCACTGCACGCGA GGATCCGCGGGCTCACCGCCTGCTGCTGCCCAGCGGCGCCCTCTTCTTTCCCCGCATCGTGCACGGGCGT CGCTCTCGGCCTGACGAGGGTGTCTACACCTGTGTGGCTCGCAACTACCTGGGAGCAGCGGCTAGCAGAA ACGCCTCTCTGGAAGTAGCTGTCCTCCGAGACGATTTCCGGCAATCTCCTGGGAACGTGGTGGTAGCAGT GGGGGAGCCAGCAGTAATGGAATGCGTGCCCCCTAAGGGTCACCCAGAGCCTTTGGTGACCTGGAAGAAG GGCAATACAAAGCTTAAGGAAGAGGAGGGAAGGATCACCATACGTGGAGGAAAGCTGATGATGTCACATA CATTCAAGAGTGATGCTGGCATGTACATGTGTGTAGCCTCCAACATGGCTGGAGAACGAGAGAGTGGGGC AGCTGAACTTGTGGTACTGGAGCGTCCCTCATTTCTGCGTAGACCAATAAACCAGGTTGTCCTGGCTGAT GCCCCTGTGAATTTCCTGTGTGAGGTGCAGGGAGATCCCCAGCCCAATCTACGCTGGCGCAAGGATGATG GGGAGCTGCCCACTGGCAGGTATGAGATTCGGAGTGACCACAGCCTTTGGATCGAGCAAGTGAGTGCTGA AGACGAGGGGACTTACACCTGTGTGGCAGAAAACAGTGTGGGCCGAGCAGAGGCATCTGGCTCCCTCAGT GTTCACGTCCCACCACAGTTTGTGACTAAGCCCCAGAACCAGACAGCAGCTCCTGGAGAAAACGTGTCTT TCCAATGTGAGACCAAAGGAAACCCACCACCTGCCATCTTCTGGCAGAAGGAAGGGAGTCAAGTCTTGCT TTTCCCTAGTCAGTCACTCCAGCCCATGGGACGCCTCTTAGTCTCTCCAAGAGGCCAGCTCAACATCACT GAAGTAAAGATCGGGGATGGTGGCTACTATGTGTGCCAGGCTGTCAGTGTGGCTGGTAGCATCCTGGCTA AGGCCCTGTTAGAGATAAAAGGAGCCTCCATAGATGGACTACCTCCCATCATTCTCCAGGGACCAGCCAA TCAGACATTGGTACTTGGCTCTTCTGTGTGGCTGCCATGCAGAGTGATTGGAAACCCTCAGCCCAATGTC CAATGGAAGAAGGATGAGACGTGGCTGCAGGGGGATAACTCACAGTTCAACTTAATGGACAACGGCACAC TATACATTGCCAGTGTACAGGAGATGGACATGGGTTTCTACAGCTGTGTGGCCAAGAGTTCCATAGGGGA GGCCACATGGAATAGCTGGCTTAGGAAGCGAGAACATTGGGGAGCATCACCAGGTCCAGCAACAGAACCC GGTAATCCTCCAGGCCCTCCCTCTCAGCCAATAGTCACAGAGGTAACCACAAACAGCATTACCTTGACCT GGAAGCCCAATCCACAGTCTGGGGCCAGAGCTACCTCCTATGTGATAGAGGCCTTCAGCCAAGCAGCTGG CAACACGTGGCGGACGGTGGCAGATGGGGTACAGCTGGAAACGCACACCGTCAGCGGCCTGCAGCCCAAT ACCATCTACCTATTCCTCGTGAGAGCTGTTGGAGCCTGGGGCCTCAGTGAACCCAGTCCTGTCTCTGAGC CTGTTCAAACCCAGGACAGCAGCCTATCTAGGCCAGTGGAGGACCCGTGGAAAGGCCAGCAAGGACTGGC TGAAGTGGCCGTGCGAATGCAGGAGCCCATAGTCCTTGGACCCAGAACTCTGCAGGTGTCCTGGACTGTA GAGGGCCCTGGCCAGCTGGTTCAGGGATTCCGTGTGTCTTGGAGGATTGCAGGCCTTGACCAGGGAAGCT GGACAGCGCTAGACGTACAGAGTCCACACAAGCAAAGTACTGTGCTAAGAGGCCTCCCCCCAGGGTCCCA AATACAGATCAAGGTGCAAGTCCAAGGCCAGGAGGGGCTGGGGGCTGAAAGCCCCTCTGTGACCCGGAGC ATTCCTGAAGAGGCCCCCAGTGGCCCCCCTCAGGGAGTGGCTGTGGCCTTGGGGGGTGATCGCAACAGCA GTATCACTGTATCCTGGGAGCCTCCACTTCCCTCCCAGCAAAATGGGGTTATCACTGAATACCAGATCTG GTGCCTGGGTAATGAAAGCCGATTCCACCTCAATCGATCTGCAGCAGGCTGGGCACGCTCTGTGATGTTC CGGGGACTGCTACCGGGCCTGCTCTATAGAGCCATAGTGGCAGCAGCTACCAGCGCTGGCGTGGGCGTGG CCAGTGCCCCAGTGCTAGTGCAGCTGCCATTCCCTCCGGTTGCGGAGCCCGGGCCTGAGGTCAGCGAGGG GCTGGCGGAACGGTTGTCTAGGGTGCTGCGGAAGCCTGCTTTCCTGGCTGGCAGCAGCGCGGCCTGTGGG GCGCTGCTTCTCGGGCTCTGCGCCGCCCTCTATCGGCGCCAGAAGCAACGCAAAGAGCTCAGTCATTATA CCGCCTCCTTTGCCTACACACCTGCAGTGTCCTTTCCACACTCAGAGGGCCTTTCTGGATCCAGTTCCAG GCCACCCATGGGCCTTGGCCCTGCTGCCTACCCATGGCTGGCAGACTCCTGGCCCCACCCATCTCGAAGT CCCTCAGCTCAGGAACCCCGGGGGAGCTGCTGCCCCAGCAATCCTGATCCAGATGATAGATACTACAATG AGGCAGGAATCTCCCTGTACTTGGCTCAGACTGCACGGGGTGCTAATGCCCCTGGCGAGGGTCCTGTCTA CAGCACCATTGACCCAGTAGGGGAAGAGCTACAGACCTTCCACGGAGGGTTCCCTCAACATTCTTCTGGG GACCCAAGCACCTGGAGCCAGTATGCTCCCCCCGAATGGAGCGAGGGGGACAGTGGAGCCAGGGGAGGCA AAGGGAAGCTTCTAGGCAAACCTGTGCAGATGCCCTCTTTGAGCTGGCCAGAAGCCCTGCCGCCACCTCC CCCTTCCTGTGAATTGAGCTGCCCAGAGGGGCCTGAGGAGGAGCTGAAGGGCAGTTCAGATCTGGAAGAG TGGTGCCCACCAATGCCTGAGAAAAAACACTTGATTGACTCACGTTCCAGTGGTGCGTGCATGGTGGCTC CAGCCCGAGGGGAAACTCCCTCCCCTACCTCTTCTTACGGACAGCAGTCCACAGCCACTCTTACCCCCTC ACCTCCTGACCCTCCCCAGCCCCCTAGCGACATCCCACATCTCCATCAGATGCCCAGGAGGGTGCCCCTT GGACCAAGTTCTCCTCTCAGCGTATCCCAGCCTGCTCTGAGTAGCCATGATGGAAGGCCTGTTGGCCTGG GTGCTGGCCCTATAGTCTCTTATCACCCTAGCCCCAGTCCTGTCCCTAGTACAGCCAGCAGTGCACCAGG GAGAACTCGCCAGGTAACTGGAGAGATGACTCCCCCACTCCATGGACACCGTGCCCGAATCCGGAAGAAA ACCAAGGCTCTTCCACATAGAAGGGAACACAGTCCTGGGGACTTGCCCCCACCACCCTTGCCACCACCAG AGGAAGAGACGAGCTGGCCCTTGGGTCTGAGAGCAGCAGGCAGCATGTCTTCCCTAGAGCGAGAGCACAG TGGGGAGAGGAGAGTGGCCCAGGCAGTGCCTCTAGGGGCCCAACCTCCAGGGGCCCAACGTGGCCCCCAC CCTGATGGTAAGGAGAGCCAGGGCAATGGGAGGGGCCTGGAGGCTTGCAGAAGTCCCCAAAGTCCCCAGA TTCAGTTGGATTCACGTGTCTGGTCAACATTGATATTGTCTCTTATCTCTTTCACTTCCTCTAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202319 representing NM_001108135 Red=Cloning site Green=Tags(s) MPRIVEQPPDLLVSRGEPATLPCRAEGRPRPNIEWYKNGARVATAREDPRAHRLLLPSGALFFPRIVHGR RSRPDEGVYTCVARNYLGAAASRNASLEVAVLRDDFRQSPGNVVVAVGEPAVMECVPPKGHPEPLVTWKK GNTKLKEEEGRITIRGGKLMMSHTFKSDAGMYMCVASNMAGERESGAAELVVLERPSFLRRPINQVVLAD APVNFLCEVQGDPQPNLRWRKDDGELPTGRYEIRSDHSLWIEQVSAEDEGTYTCVAENSVGRAEASGSLS VHVPPQFVTKPQNQTAAPGENVSFQCETKGNPPPAIFWQKEGSQVLLFPSQSLQPMGRLLVSPRGQLNIT EVKIGDGGYYVCQAVSVAGSILAKALLEIKGASIDGLPPIILQGPANQTLVLGSSVWLPCRVIGNPQPNV QWKKDETWLQGDNSQFNLMDNGTLYIASVQEMDMGFYSCVAKSSIGEATWNSWLRKREHWGASPGPATEP GNPPGPPSQPIVTEVTTNSITLTWKPNPQSGARATSYVIEAFSQAAGNTWRTVADGVQLETHTVSGLQPN TIYLFLVRAVGAWGLSEPSPVSEPVQTQDSSLSRPVEDPWKGQQGLAEVAVRMQEPIVLGPRTLQVSWTV EGPGQLVQGFRVSWRIAGLDQGSWTALDVQSPHKQSTVLRGLPPGSQIQIKVQVQGQEGLGAESPSVTRS IPEEAPSGPPQGVAVALGGDRNSSITVSWEPPLPSQQNGVITEYQIWCLGNESRFHLNRSAAGWARSVMF RGLLPGLLYRAIVAAATSAGVGVASAPVLVQLPFPPVAEPGPEVSEGLAERLSRVLRKPAFLAGSSAACG ALLLGLCAALYRRQKQRKELSHYTASFAYTPAVSFPHSEGLSGSSSRPPMGLGPAAYPWLADSWPHPSRS PSAQEPRGSCCPSNPDPDDRYYNEAGISLYLAQTARGANAPGEGPVYSTIDPVGEELQTFHGGFPQHSSG DPSTWSQYAPPEWSEGDSGARGGKGKLLGKPVQMPSLSWPEALPPPPPSCELSCPEGPEEELKGSSDLEE WCPPMPEKKHLIDSRSSGACMVAPARGETPSPTSSYGQQSTATLTPSPPDPPQPPSDIPHLHQMPRRVPL GPSSPLSVSQPALSSHDGRPVGLGAGPIVSYHPSPSPVPSTASSAPGRTRQVTGEMTPPLHGHRARIRKK TKALPHRREHSPGDLPPPPLPPPEEETSWPLGLRAAGSMSSLEREHSGERRVAQAVPLGAQPPGAQRGPH PDGKESQGNGRGLEACRSPQSPQIQLDSRVWSTLILSLISFTSSK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001108135
ORF Size	3915 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001108135.1, NP_001101605.1
RefSeq Size	5682 bp
RefSeq ORF	3918 bp
Locus ID	315564
MW	139.9 kDa

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