Sirt5 (NM_001004256) Rat Tagged Lenti ORF Clone
CAT#: RR202333L4
- LentiORF®
Lenti ORF clone of Sirt5 (mGFP-tagged ORF) - Rat sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (Sirt5), (10 ug)
"NM_001004256" in other vectors (3)
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CNY 6,650.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | mGFP |
Synonyms | MGC93823 |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RR202333).
|
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001004256 |
ORF Size | 930 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001004256.1 |
RefSeq Size | 1370 bp |
RefSeq ORF | 933 bp |
Locus ID | 306840 |
UniProt ID | Q68FX9 |
Gene Summary | NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202333 | Sirt5 (untagged ORF) - Rat sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (Sirt5), (10 ug) |
CNY 2,640.00 |
|
RR202333 | Sirt5 (Myc-DDK-tagged ORF) - Rat sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (Sirt5), (10 ug) |
CNY 3,990.00 |
|
RR202333L3 | Lenti ORF clone of Sirt5 (Myc-DDK-tagged ORF) - Rat sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae) (Sirt5), (10 ug) |
CNY 6,080.00 |