Nup155 (NM_053952) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202335 representing NM_053952 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCATCTATGCTGGGCTCGATGATGGTGGCCTCTACGTCGGCCCCCTCCCTGCAAGAAGCTTTGGAAA ATGCGGGGAGGCTTATCGACCGACAGTTGCAAGAAGACCGCATGTACCCGGACCTTTCTGAGCTGCTCAT GGTGTCGGCCCCAAACAGTCCTACTGTTTCTGGAATGTCTGATATGGATTACCCTCTGCAAGGACCAGGT CTACTGTCAGTACCGAGCCTTCCAGAGATCAGCACCATCCGAAGAGTTCCCCTCCGCCTGAGCTGGTTGA ACAGTTTGGACACATGCAGTGTAACTGCAATGATGGGTGTTTTCCCTCCTATCAGCAGAGCTTGGCTTAC AATTGACAGTGACATATTCATGTGGAACTATGAAGACGGAGGTGATCTTGCCTATTTTGATGGACTCAGT GAGACAATTCTTGCTGTGGGGCTTGTGAAACCAAAAGCTGGAATCTTTCAACCTCATGTAAGACATTTGT TGGTTTTGGCAACACCTGTGGATATAGTAATTCTTGGACTCAGCTATGCTAATGTTCAGACAGGCTCTGG CATCCTTAATGACAGTGTGTGTGGTGGCCTGCAGCTGCTTCCAGATCCTTTGTACTCCCTTCCCACGGAC AACACTTACCTTTTAACTATCACCTCCACGGATAATGGCAGGATTTTTTTGGCTGGAAAGGACGGCTGTT TGTATGAAGTAGCATACCAAGCAGAAGCAGGCTGGTTCAGTCAAAGATGTAGGAAGATAAACCACTCCAA GAGCTCGCTCTCCTTCCTCGTGCCTTCCTTGCTGCAGTTCACCTTCTCAGAAGATGATCCTATTGTTCAA ATTGAAATTGATAATTCCAGAAATATTTTGTATACCCGATCGGAGAAAGGAGTGATACAGGTTTATGATT TGGGCCATGATGGACAGGGGATGAGTAGAGTTGCTTCAGTTTCACAGAATGCCATTGTCTGTGCTGCTGG GAACATTGCTAGGACTATCGATCGTTCCGTTTTTAAGCCAATTGTTCAAATAGCTGTGATTGAGAACTCT GAATCATTGGACTGTCAGTTACTGGCTGTCACTCATGCAGGTGTGAGGTTGTATTTCAGCACTTGTCCGT TTAGACAGCCATTAGCTCGGCCCAATACACTGACACTGGTTCACGTCCGGTTACCTCCTGGCTTCTCAGC GTCTTCAACAGTTGAAAAGCCTTCAAAAGTACATAAAGCACTTTACAGTAAAGGAATTTTACTGATGACA GCCTCAGAAAATGAAGATAATGACATTTTATGGTGTGTCAACCATGATACATTTCCTTTCCAAAAGCCAA TGATGGAAACTCAGATGACAACTCGTGTTGATGGCCATTCCTGGGCTCTTTCTGCAATAGATGAATTAAA AGTAGATAAAATAATCACGCCTTTAAATAAAGATCATATTCCAATAACTGACTCCCCAGTTGTTGTACAA CAGCACATGTTGCCTCCAAAGAAATTCGTTCTCCTCTCAGCACAGGGTAGTCTTATGTTTCACAAACTTA GACCCGTAGATCAGCTGAGACATCTGCTTGTGAGTAACGTGGGAGGGGATGGAGAAGAGATTGAGAGATT CTTCAAATTACATCAGGAAGACCAAGCTTGTGCGACGTGCCTTATTCTTGCTTGTTCCACCGCTGCCTGT GACAGAGAGGTGTCTGCCTGGGCTACGAGGGCATTCTTTAGGTATGGTGGTGAGGCACAGATGAGATTTC CAGCTACTCTTCCCACTCCGAGTAACGTTGGTCCCATCCTGGGCTCTCCTATGTATTCTAGTTCTCCTGT TCCTACTGGGAGTCCCTATCCAAATCCTTCCTCTTTGGGGACACCATCCCATGGTGCTCAGCCTCCTACC ATGTCAACCCCAATGTCTGCTGTTGGAAACCCAGCCATGCAGGCTGCAAGCCTGAGTGGTTTGACTGGAC CAGAGATTGTGTACTCAGGAAAACACAACGGTATTTGCATTTACTTTTCTCGAATCATGGGAAATATTTG GGATGCTAGCTTAGTTGTTGAAAGAGTATTCAAGAGTTCCAACAGGGAGATCACTGCAATTGAAAGCAGT GTGCCTATCCAGCTGCTGGAGTCAGTGCTACAGGAACTGAAGGGTTTGCAGGAATTTCTAGACAGAAATT CTCAGTTTTCAGGAGGACCACTAGGAAATCCAAATACCACTGCCAAAGTGCAGCAGAGGCTGCTTGGAGT CATGCGTCCCGAGAATGGAAACACCCAGCAAATGCAACAGGAGCTGCAGAGGAAGTTTCATGAGGCTCAA CTGAGTGAGAAGATTTCACTTCAGGCAATCCAGCAGCTAGTTAGAAAATCGTACCAAGCTCTGGCTTTGT GGAAACTCCTTTGTGAACATCAGTTTACTGTCATTGTAGGAGAACTTCAAAAGGAATTTCAAGAGCAGCT GAAGATCACTACCTTTAAGGATCTGGTAATCAGGGAGAAAGAGGTCACTGGAGCACTAATTGCTTCTCTC ATCAACTGCTACATCAGAGACAATGCTGCTGTAGATGGCATTAGTTTACATCTGCAGGACACCTGTCCGC TTCTGTATAGCACAGATGATGCAGTTTGTTCCAAGGCAAATGAACTTCTTCAGCGTTCCCGGCAAGTTCA GAGTAAGAGCGAGAGAGAGAGGATGTTGAGGGAGTCTCTGAAGGAGTACCAGAAAATCAGCAACCAAGTA GACCTCCCCAGTGTGTGTGCTCAGTATCGGCAAGTGCGCTTTTATGAGGGTGTGGTGGAACTGTCTCTTA CTGCTGCGGAGAAGAAAGATCCTCAGGGTCTTGGACTCCATTTCTATAAGCATGGGGAACCGGAAGAGGA TGTGGTTGGTCTTCAGACTTTCCAAGAGAGATTAAACAGTTACAAGTGCATCACAGACACTCTTCAAGAA CTGGTAAATCAAAGTAAGGCAGCTCCTCAGTCTCCTAGTGTACCCAAAAAGCCTGGTCCTCCAGTGTTGT CATCCGATCCCAACATGCTGAGCAATGAAGAGGCAGGACATCACTTTGAACAAATGCTTAAACTGGCTCA GCGATCCAAGGATGAGCTCTTCAGTATTGCTCTCTATAACTGGCTAATCCAAGCTGACCTTGCAGATAAA CTGCTACAGATTGCATCTCCATTTCTTGAGCCACATCTTGTCCGAATGGCCAAAGTGGATCAAAACCGAG TTCGCTATATGGATTTACTCTGGAGGTATTATGAGAAGAACAGGAGTTTCAGCAGTGCTGCTCGGGTCCT GTCCAAGCTGGCCGACATGCACAGCACAGAAATTTCACTGCAGCAGCGACTAGAATACATTGCTCGAGCC ATTCTTAGTGCAAAAAGTTCCACTGCCATTTCATCCATAGCTGCAGATGGTGAATTCCTACATGAACTAG AAGAGAAAATGGAAGTTGCTAGGATCCAACTTCAGATACAAGAGACACTACAAAGGCAGTACTCCCATCA TTCTTCAGTGCAGGATGCAATTTCTCAGCTGGATTCTGAGCTCATGGACATTACTAAGCTTTATGGAGAA TTTGCTGACCCATTTAAACTTGCAGAATGTAAACTTGCAATCATTCATTGTGCTGGTTATTCAGATCCCA TATTGGTACACACACTTTGGCAAGATATCATAGAGAAAGAATTGAGTGATAGCGTAACATTGAGTTCATC AGATCGAATGCATGCTCTTAGCCTCAAGCTTGTTCTCCTTGGCAAAATCTATGCTGGCACACCTCGATTT TTCCCTCTGGATTTCATTGTGCAGTTCTTGGAGCAGCAAGTTTGTACTTTAAACTGGGATGTGGGATTTG TGATACAGACCATGAATGAAATCGGTGTGCCTTTACCTAGGCTACTAGAAGTTTATGATCAGCTCTTCAA ATCAAGGGATCCATTCTGGAACAGAGTGAAAAGTCCACTGCACCTTTTGGATTGTATCCATGTACTGTTG ACAAGATATGTTGAGAATCCTAGCCTAGTTTTAAATTGTGAGAGGAGAAGGTTTACAAATCTCTGCCTGG ATGCCGTCTGTGGTTATCTGGTTGAACTTCAGTCAATGAGCTCTTCGGTGGCAGTACAAGCCATCACTGG GAATTTTAAATCTCTCCAAGCGAAGTTGGAACGGCTCCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202335 representing NM_053952 Red=Cloning site Green=Tags(s) MPSMLGSMMVASTSAPSLQEALENAGRLIDRQLQEDRMYPDLSELLMVSAPNSPTVSGMSDMDYPLQGPG LLSVPSLPEISTIRRVPLRLSWLNSLDTCSVTAMMGVFPPISRAWLTIDSDIFMWNYEDGGDLAYFDGLS ETILAVGLVKPKAGIFQPHVRHLLVLATPVDIVILGLSYANVQTGSGILNDSVCGGLQLLPDPLYSLPTD NTYLLTITSTDNGRIFLAGKDGCLYEVAYQAEAGWFSQRCRKINHSKSSLSFLVPSLLQFTFSEDDPIVQ IEIDNSRNILYTRSEKGVIQVYDLGHDGQGMSRVASVSQNAIVCAAGNIARTIDRSVFKPIVQIAVIENS ESLDCQLLAVTHAGVRLYFSTCPFRQPLARPNTLTLVHVRLPPGFSASSTVEKPSKVHKALYSKGILLMT ASENEDNDILWCVNHDTFPFQKPMMETQMTTRVDGHSWALSAIDELKVDKIITPLNKDHIPITDSPVVVQ QHMLPPKKFVLLSAQGSLMFHKLRPVDQLRHLLVSNVGGDGEEIERFFKLHQEDQACATCLILACSTAAC DREVSAWATRAFFRYGGEAQMRFPATLPTPSNVGPILGSPMYSSSPVPTGSPYPNPSSLGTPSHGAQPPT MSTPMSAVGNPAMQAASLSGLTGPEIVYSGKHNGICIYFSRIMGNIWDASLVVERVFKSSNREITAIESS VPIQLLESVLQELKGLQEFLDRNSQFSGGPLGNPNTTAKVQQRLLGVMRPENGNTQQMQQELQRKFHEAQ LSEKISLQAIQQLVRKSYQALALWKLLCEHQFTVIVGELQKEFQEQLKITTFKDLVIREKEVTGALIASL INCYIRDNAAVDGISLHLQDTCPLLYSTDDAVCSKANELLQRSRQVQSKSERERMLRESLKEYQKISNQV DLPSVCAQYRQVRFYEGVVELSLTAAEKKDPQGLGLHFYKHGEPEEDVVGLQTFQERLNSYKCITDTLQE LVNQSKAAPQSPSVPKKPGPPVLSSDPNMLSNEEAGHHFEQMLKLAQRSKDELFSIALYNWLIQADLADK LLQIASPFLEPHLVRMAKVDQNRVRYMDLLWRYYEKNRSFSSAARVLSKLADMHSTEISLQQRLEYIARA ILSAKSSTAISSIAADGEFLHELEEKMEVARIQLQIQETLQRQYSHHSSVQDAISQLDSELMDITKLYGE FADPFKLAECKLAIIHCAGYSDPILVHTLWQDIIEKELSDSVTLSSSDRMHALSLKLVLLGKIYAGTPRF FPLDFIVQFLEQQVCTLNWDVGFVIQTMNEIGVPLPRLLEVYDQLFKSRDPFWNRVKSPLHLLDCIHVLL TRYVENPSLVLNCERRRFTNLCLDAVCGYLVELQSMSSSVAVQAITGNFKSLQAKLERLH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053952
ORF Size	4170 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053952.1, NP_446404.1
RefSeq Size	4311 bp
RefSeq ORF	4173 bp
Locus ID	117021
UniProt ID	P37199
MW	155 kDa
Gene Summary	nuclear pore complex protein [RGD, Feb 2006]

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