Smc1b (NM_001130498) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Smc1l2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202488 representing NM_001130498 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCACCTTGAGTTGCTGCTCGTGGAGAATTTCAAGTCGTGGCGAGGCCGCCAGGTCATCGGCCCTT TCAAGAGGTTCACCTGTATCATCGGCCCCAACGGCTCGGGAAAGTCTAATGTAATGGATGCACTTAGTTT TGTAATGGGAGAAAAAACAACTAATTTAAGAGTAAAAAACATTCAAGAACTTATTCATGGAGCACACATT GGAAAACCTGTTTCTTCTTCTGCAAGTGTGACCATTATATACGTAGAAGACAGTGGAGAAGAGAAAACAT TCACAAGGATTATCCGAGGGGGGTGCTCAGAATTTCATTTTGGGGATAAACCTGTGAGTCGTTCTGTGTA TGTAGCCCAGTTGGAAAACATAGGCATAATAGTCAAAGCACAGAACTGTCTAGTTTTTCAGGGAACTGTA GAGTCAATATCCATGAAAAAACCCAAAGAGAGAACCCAATTTTTTGAAGAAATCAGTACTTCAGGAGAGT TTATAGGAGAATATGAGGCAAAGAAAAAGAAGTTACAAAAAGCCGAAGAAGATGCACAATTTCATTTTAA CAGGAAAAAAAATGTAGCTGCAGAACGGAAGCATGCAAAAATAGAGAAAGAAGAGGCAGAACATTATCAA AGTCTCCTTGAAGAATTGAAAACAAACAAGATACAACTGATGCTTTTCCAACTATATTATAATGAGGAAA AAATCAATGTTTTAAACACTGAATTAGAGCATATGGACAGGAATTTAAGTGTTGTAAAAGACACTCTTTC TCACCATGAAAACATAATTAAAGCTAAGAAAAAGGATTATGGAATGTTAACTAGACAACTACAGCAAACA GCAAAAGAACTGAAATCTGTCGAAGCAGTTTTAAATCAGAAGAGGCCTCAGTACATTAAAGCTAAAGAAA ACACTTCTCACCATCTAAAAAAATTGGATTTGTCTAAGAAATTGATAACAGACAATGAAAAACAATGTGC TAAGCAGGAAGATGGCATTCGAGCCTTAGTGGCAGAACTGGTTGATTTAGATAGAGCATGGAGAAGTTTT GAAAAGCAGATGGAAGAGAAAATCTTACACAAAGGAAGAGACATTGAATTGGAAAATAGTCAGCTGGACC GTTATAAAGAGCTTAAGGAACAAGTCAGACGGAGGGTAGGTATAATGACACAACAACTGGAAAAACTACA GTGGGAACAGAAGGCAGAAAAAGAAAGACTGGCATTTGAAAGGAGGAGGCATGGAGACACTCAGGGAAAT CTAAAACAAATAAAAGAACAAATAGAAGAGCATAAAAAACGAATAGAGAAATTGGAGGAATATACAAAGA CGTGCATGGATTGCTTGGAAGATAAAAAACAGCAAGAAGAAGCCCTGATAAAAGAAATTGACAATACAAA ATCAAGAATGTCTGAAGTTAATGAAGAATTGAGTCTTATTAGAAATGAATTGCAGAATGCTGGAATTGAT AACCATGAAGGAAAACGTCAGCGGAAAAGAGCAGAAGTTCTCGAACACCTTAAAAGACTTTACCCAGATT CTGTGTTTGGAAGACTTCTTGATCTATGTCATCCTATTCATAAGAAATACCAGCTGGCTGTGACTAAGCT TTTTGGCCGGTACATGGTTGCCATTGTTGTTGCCTCAGAAAAGGTAGCAAAAGATTGTATTCGGTTTCTG AAGGAGGAAAGAGCTGAACCTGAGACATTCCTTGCTCTAGATTACCTTGATATCAAGCCAATCAATGAAC GACTAAGGGAAATTAAAGGCTGTAAGATGATGATCGATGTTATAAAGACCCAGTTTCCTCAGCTGAAGAA AGTGATTCAGTTTGTTTGTGGAAATGGCCTTGTCTGTGAGACTGTGGAAGAGGCAAGACATATTGCATTT GGTGGACCTGAAAGACGAAAGGCAGTAGCACTTGATGGAACACTGTTTTTGAAATCTGGAGTGATCTCAG GAGGGTCAAGTGACTTAAAGCACAAAGCTCTGTGTTGGGATGAGAAGGAGTTACACAGTCTAAGAGATAA AAGAAACCAACTTGTCCAAGAGCTAAAGGAGTTAATGAAGACGCTCCGCAAGGAAACAGATCTGAAACAA ATACAGGCATTAGTACAAGGAACCAATACACGACTCAAATATTCACAAAATGAACTAGAGATGATTAAAA AGAAGCACCTTGCTACATTTTACCGGGAACAATCTCAGCTACAAAGTGAATTACTGAATATTGATTCTCA GTGTACAATGTTGAGCGAAGGAATCAACAAACAGCAACAAAAGATTGAAGAATTTCAAGATAAGATAGAC AAGGTAGAAGATGACATATTCCAAGACTTCTGTGAAGAAATTGGTGTGGAAAATATCCGTGAATTTGAGA ACAAACATGTTAAACAGCAGCAAGAAAATGATCAAAAAAGATTAGAGTTTGAAAAACAAAAAACTCGGCT CAATATTCAGCTTGAATATAGTCGAAATCAACTTAAGAAGAAACTGAATAAGATCGACACATTAAAAACA ACAATCCAGAAGGGCAGAGAAGACATTGATAACCTAAAGAAGACCGAAGAAGAGTGTCTGAAAATTGTTG AAGAACTCATGGTGAAACAAGAACAAGTTAAGGAAGTGCTTGCCACACAGAGTTCCAACATTGAGAAAAT TCATATTCAAATTGAAGAGGAACGCAAGAAGTTTTTGGCTGTTGATAGGGAAGTTGGAAAATTACAGAAG GAAGTTGTAATCATTCAGGGTTCTTTGGAACAGAAACAACTAGAGAAGCATAACTTGCTCCTGGACTGCA AAGTTCAAGACATTGACATAAGTCTCATGCTGGGGTCATTGGAGGACATCATTGAAGTGGAGCTAACTGA AACAGAAAGTACCCAGGCAACGGCTGATATCTATGAAAAAGAAGCATCCATCCAAATAGACTACAGCCCT CTAAGGGAGGATTGGAAGGCTCTACAATCAGATAAGGAGGTGGAGGCCCACCTCACACTCCTTCTACAGC AAGTAGCATCCCAAGAAAATACTCTACTGAAGACTGCAGCTCCAAACCTGCGAGCACAGGAGAACTTAAA GGCTGTCAGAGACAAATTTCAAGAATCTGCAGATGCTTTTGAGGCCAGCAGAAAGGAAGCCAGAATATGT AGGCAAGAGTTTGAACAGGTGAAAAGACGGAGGTACGATGCTTTCAGTCAGTGTTTTGAGCACATCTCGG TCTCAATTGATCAAATCTACAAGAAGCTCTGCAGGAACAACAGTGCCCAGGCATTTCTTAGCCCAGAGAA CCCTGAAGAACCTTACTTAGATGGAATTAGCTATAATTGTGTGGCTCCAGGCAAACGGTTCATGCCTATG GACAACTTGTCAGGGGGAGAAAAGTGTGTGGCTGCTCTGGCTCTTCTGTTTGCTGTACACAGTTTTCGAC CTGCTCCATTCTTTGTATTAGATGAAGTAGATGCAGCCCTGGACAATACTAACATCGGCAAAGTCTCAGG TTACATCAAAGAGCAGAGTCAGGAACAATTTCAGATGATAATCATTTCCCTGAAAGAGGAGTTTTACTCC AGAGCTGATGCACTGATAGGCGTCTACCCAGAGCACGATGAATGCATGTTCAGCCATGTGTTGACTCTGG ACCTTTCCAAGTATCCAGACACTGAAGACCAAGAAGGCAGCAGGAGCCACCGGAAACCCAGAGCACGAGT ATCACAGTCCCCGCAGTCTCCCCAGTCTCGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202488 representing NM_001130498 Red=Cloning site Green=Tags(s) MGHLELLLVENFKSWRGRQVIGPFKRFTCIIGPNGSGKSNVMDALSFVMGEKTTNLRVKNIQELIHGAHI GKPVSSSASVTIIYVEDSGEEKTFTRIIRGGCSEFHFGDKPVSRSVYVAQLENIGIIVKAQNCLVFQGTV ESISMKKPKERTQFFEEISTSGEFIGEYEAKKKKLQKAEEDAQFHFNRKKNVAAERKHAKIEKEEAEHYQ SLLEELKTNKIQLMLFQLYYNEEKINVLNTELEHMDRNLSVVKDTLSHHENIIKAKKKDYGMLTRQLQQT AKELKSVEAVLNQKRPQYIKAKENTSHHLKKLDLSKKLITDNEKQCAKQEDGIRALVAELVDLDRAWRSF EKQMEEKILHKGRDIELENSQLDRYKELKEQVRRRVGIMTQQLEKLQWEQKAEKERLAFERRRHGDTQGN LKQIKEQIEEHKKRIEKLEEYTKTCMDCLEDKKQQEEALIKEIDNTKSRMSEVNEELSLIRNELQNAGID NHEGKRQRKRAEVLEHLKRLYPDSVFGRLLDLCHPIHKKYQLAVTKLFGRYMVAIVVASEKVAKDCIRFL KEERAEPETFLALDYLDIKPINERLREIKGCKMMIDVIKTQFPQLKKVIQFVCGNGLVCETVEEARHIAF GGPERRKAVALDGTLFLKSGVISGGSSDLKHKALCWDEKELHSLRDKRNQLVQELKELMKTLRKETDLKQ IQALVQGTNTRLKYSQNELEMIKKKHLATFYREQSQLQSELLNIDSQCTMLSEGINKQQQKIEEFQDKID KVEDDIFQDFCEEIGVENIREFENKHVKQQQENDQKRLEFEKQKTRLNIQLEYSRNQLKKKLNKIDTLKT TIQKGREDIDNLKKTEEECLKIVEELMVKQEQVKEVLATQSSNIEKIHIQIEEERKKFLAVDREVGKLQK EVVIIQGSLEQKQLEKHNLLLDCKVQDIDISLMLGSLEDIIEVELTETESTQATADIYEKEASIQIDYSP LREDWKALQSDKEVEAHLTLLLQQVASQENTLLKTAAPNLRAQENLKAVRDKFQESADAFEASRKEARIC RQEFEQVKRRRYDAFSQCFEHISVSIDQIYKKLCRNNSAQAFLSPENPEEPYLDGISYNCVAPGKRFMPM DNLSGGEKCVAALALLFAVHSFRPAPFFVLDEVDAALDNTNIGKVSGYIKEQSQEQFQMIIISLKEEFYS RADALIGVYPEHDECMFSHVLTLDLSKYPDTEDQEGSRSHRKPRARVSQSPQSPQSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001130498
ORF Size	3741 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001130498.1, NP_001123970.1
RefSeq Size	4026 bp
RefSeq ORF	3744 bp
Locus ID	300121
MW	144.7 kDa

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