Magi1 (NM_001030045) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Baiap1; BAP-1; MAGI-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR202632 representing NM_001030045 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAAGTGATCCAGAAGAAGAACCACTGGACTGGCCGCGTTCACGAATGCACCGTGAAGCGGGGAC CCCAGGGCGAGCTGGGGGTGACGGTGCTGGGGGGCGCGGAGCATGGGGAGTTTCCGTACGTGGGGGCGGT GGCGGCGGCCGAGGCGGCGGGGCTTCCCGGCGGTGGCGAGGGGCCGAAGCTGGCTGAAGGTGAGCTGCTG CTGGAGGTGCAGGGGGTCCGGGTGTCCGGCTTGCCCCGCTATGACGTGCTGGGAGTCATCGACAGCTGCA AGGAGGCCGTCACCTTCAAAGCCGTCAGACAAGGAGGAAGGCTCAACAAGGACCTACGACATTTCCTCAA CCAACGGTTCCAGAAGGGGTCTCCAGATCATGAGCTCCAGCAGACCATAAGGGACAACCTCTACCGCCAT GCTGTGCCTTGCACAACCCGGTCTCCCAGAGAAGGAGAAGTGCCTGGCGTCGATTACAGCTTTCTGACTG TGAAGGAGTTCCTGGACCTCGAGCAGAGTGGGACCCTGTTGGAAGTCGGCACCTATGAAGGAAACTATTA TGGGACACCCAAACCTCCCAGCCAGCCAGTCAGTGGGAAAGTGATCACGACGGATGCCTTGCACAGCCTG CAGTCTGGCTCCAAACAGTCGACCCCTAAGCGAACAAAATCCTACAATGATATGCAAAATGCTGGCATAG TCCACACGGAGAATGAGGAAGAGGAGGATGTTCCTGAAATGAACAGTAGCTTCACAGCCGACTCTGGAGA CCAGGACGAGCCCACTCTCCAAGAAGCGACGCTCCCGCCTGTGAATAGTAGCGCCCTTGCTGCTCCCATC ACGGACCCTTCTCAGAAGTTCCCTCAGTACCTACCTCTTTCTGCAGAGGATAATTTAGGTCCTCTACCTG AAAACTGGGAGATGGCCTATACTGAAAATGGAGAAGTCTATTTTATAGACCACAACGCGAAAACAACATC ATGGTTAGACCCTCGGTGCCTGAACAAACAGCAGAAGCCTCTGGAAGAATGTGAAGATGATGAAGGGGTA CACACCGAGGAGCTGGACAGTGAACTAGAGCTGCCTGCTGGCTGGGAAAAGATTGAAGACCCTGTCTACG GTGTCTACTATGTAGACCACATCAACAGGAAGACGCAATATGAAAACCCAGTCCTAGAAGCCAAAAGGAA GAGACAGCTTGAACAACAGCAGCAGCAGCAGCAGCACCAGCAGCAGCCGCAGCAGCCTCAGCCACCGCAG CCAGAAGAGTGGACAGAAGATCATGCATCTGTTGTGCCTCCTGTTGCTCCTTCCCATCCCCCAAGCAACC CGGAGCCAGCCAGGGAAGCTCCACTTCAGGGCAAACCTTTTTTTACAAGAAACCCTTCTGAGCTGGAAGG CAAGTTCATTCACACAAAGCTACGGAAAAGCAGTCGAGGCTTTGGCTTCACGGTGGTTGGAGGGGACGAA CCTGATGAGTTCCTGCAGATCAAGAGCCTTGTCCTAGATGGTCCTGCTGCACTGGATGGCAAGATGGAGA CAGGAGATGTAATCGTCAGTGTGAATGACACCTGTGTTTTGGGACACACACATGCTCAAGTTGTGAAAAT TTTCCAGTCCATTCCCATTGGTGCCAGTGTGGACCTTGAACTCTGCAGAGGTTATCCATTGCCTTTTGAC CCGGATGACCCTAACACAAGTTTAGTGACCTCGGTGGCCATTTTGGACAAAGAACCAATAATTGTAAACG GACAAGAGACCTATGATTCACCAGCAAGCCACAGTAGTAAAACGGGCAAAGTCAGCAACATGAAGGATGC CAGGCCAAGCAGCCCCGCAGACGTGGCTTCCAACAGTTCTCATGGGTATCCCAACGACACAGTCTCCTTG GCTTCCTCCATAGCCACCCAGCCAGAGCTAATAACCGTTCACATAGTCAAAGGGCCAATGGGATTTGGCT TTACGATCGCAGATAGTCCCGGTGGGGGTGGCCAAAGAGTGAAGCAGATTGTTGACAGTCCGCGCTGCAG AGGCCTCAAAGAAGGGGATCTGATTGTGGAGGTGAATAAGAGGAACGTGCAGGCCCTGACGCACAATCAA GTCGTGGATATGCTGATTGAGTGTCCAAAGGGAAGTGAGGTCACACTGTTGGTGCAGCGAGGAGGGCTAC CAGTTCCCAAGAAGAGTCCAAAGTCGCAGCCACTGGAGAGGAAAGACAGCCAGAACAGCTCCCAGCACAG TGTGTCCAGCCTCCGGAGCCTGCACACAGCTTCCCCGAGCCACAGTGCACAGGTGCTCCCTGAGTACCCA CCTGCAGACGTCCCTGCTCCAGATCAGACCGACAGCTCTGGGCAGAAAAAGCCAGATCCCTTTAAAATCT GGGCCCAGTCGAGGAGCATGTATGAAGACCGACCTATGTCACCTTCGCCTGCGTCAGGACTGAGCAAGGG TGAAAGAGACAGAGAGATCAATTCCACGAATTTTGGAGAATGTCAGATTCCAGATTACCAGGAGCAGGAC ATCTTCCTCTGGAGAAAAGAGACCGGTTTTGGATTTAGGATTCTGGGTGGAAATGAGCCAGGGGAACCCA TTTATATCGGTCACATCGTACCACTGGGTGCTGCTGACACAGACGGCCGCCTGAGGTCTGGAGATGAATT AATCTGTGTGGATGGGACACCAGTAATTGGGAAATCACACCAGCTCGTGGTCCAGCTTATGCAACAAGCT GCCAAGCAAGGCCACGTCAATCTCACAGTGAGGCGTAAAGTGGTGTTTACGGTCCCCAAAGCAGAGAACG AGGTGCCCTCACCAGCCTCATCGCACCACAGTAGCAACCAGCCAGCGTCCCTGACGGAAGAGAAGCGCAC ACCGCAGGGCAGCCAGAACTCGCTGAACACCGTGAGCTCAGGCAGCGGCAGCACCAGCGGCATTGGCAGT GGTGGTGGCGGAGGCAGCGGCGTGGTGAGCGCTGTGCTGCAGCCATATGATGTGGAGATTCGGCGTGGGG AGAACGAGGGCTTTGGGTTTGTCATCGTGTCCTCCGTGAGCAGGCCCGAAGCGGGCACCACCTTCGCAGG CAATGCATGTGTGGCTATGCCTCACAAAATAGGTCGGATTATTGAGGGGAGCCCTGCTGACCGCTGTGGC AAGCTGAAAGTAGGAGACCGGATCTTGGCGGTAAATGGATGTTCCATCACCAACAAATCCCATTCTGACA TTGTCAACCTAATCAAAGAAGCAGGCAACACAGTGACTCTCCGCATCATCCCCGGGGATGAGTCCTCCAA TGCCACACTGCTGACTAATGCGGAGAAGATTGCCACCATCACCACCACTCATGCCCCCTCACAACAGGGG ACCCAGGAAACAAGGACCACCACCAAACCAAAGCCGGATTCTCAGTTTGAGTTCAAAGGACCGCAGGCTA CACAGGAGCAAGATTTTTACACTGTGGAATTGGAAAGAGGAGCCAAGGGATTTGGCTTTAGTCTTCGAGG AGGGCGAGAGTATAACATGGACCTTTATGTTCTGCGCTTAGCAGAGGATGGTCCTGCAGAAAGATGCGGA AAGATGAGGATTGGCGATGAAATTCTAGAGATCAATGGTGAGACCACCAAAAACATGAAACACTCTCGGG CCATAGAACTGATCAAGAATGGCGGCCGCAGAGTCCGTCTGTTTCTGAGGCGGGGAGACGGCTCGGTCCC AGAATATGGTGGGTCAAACTATGAAAACATCCCTTCCTTCCCTGGCATGACTCCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR202632 representing NM_001030045 Red=Cloning site Green=Tags(s) MSKVIQKKNHWTGRVHECTVKRGPQGELGVTVLGGAEHGEFPYVGAVAAAEAAGLPGGGEGPKLAEGELL LEVQGVRVSGLPRYDVLGVIDSCKEAVTFKAVRQGGRLNKDLRHFLNQRFQKGSPDHELQQTIRDNLYRH AVPCTTRSPREGEVPGVDYSFLTVKEFLDLEQSGTLLEVGTYEGNYYGTPKPPSQPVSGKVITTDALHSL QSGSKQSTPKRTKSYNDMQNAGIVHTENEEEEDVPEMNSSFTADSGDQDEPTLQEATLPPVNSSALAAPI TDPSQKFPQYLPLSAEDNLGPLPENWEMAYTENGEVYFIDHNAKTTSWLDPRCLNKQQKPLEECEDDEGV HTEELDSELELPAGWEKIEDPVYGVYYVDHINRKTQYENPVLEAKRKRQLEQQQQQQQHQQQPQQPQPPQ PEEWTEDHASVVPPVAPSHPPSNPEPAREAPLQGKPFFTRNPSELEGKFIHTKLRKSSRGFGFTVVGGDE PDEFLQIKSLVLDGPAALDGKMETGDVIVSVNDTCVLGHTHAQVVKIFQSIPIGASVDLELCRGYPLPFD PDDPNTSLVTSVAILDKEPIIVNGQETYDSPASHSSKTGKVSNMKDARPSSPADVASNSSHGYPNDTVSL ASSIATQPELITVHIVKGPMGFGFTIADSPGGGGQRVKQIVDSPRCRGLKEGDLIVEVNKRNVQALTHNQ VVDMLIECPKGSEVTLLVQRGGLPVPKKSPKSQPLERKDSQNSSQHSVSSLRSLHTASPSHSAQVLPEYP PADVPAPDQTDSSGQKKPDPFKIWAQSRSMYEDRPMSPSPASGLSKGERDREINSTNFGECQIPDYQEQD IFLWRKETGFGFRILGGNEPGEPIYIGHIVPLGAADTDGRLRSGDELICVDGTPVIGKSHQLVVQLMQQA AKQGHVNLTVRRKVVFTVPKAENEVPSPASSHHSSNQPASLTEEKRTPQGSQNSLNTVSSGSGSTSGIGS GGGGGSGVVSAVLQPYDVEIRRGENEGFGFVIVSSVSRPEAGTTFAGNACVAMPHKIGRIIEGSPADRCG KLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVTLRIIPGDESSNATLLTNAEKIATITTTHAPSQQG TQETRTTTKPKPDSQFEFKGPQATQEQDFYTVELERGAKGFGFSLRGGREYNMDLYVLRLAEDGPAERCG KMRIGDEILEINGETTKNMKHSRAIELIKNGGRRVRLFLRRGDGSVPEYGGSNYENIPSFPGMTP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001030045
ORF Size	3765 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001030045.1, NP_001025216.1
RefSeq Size	3768 bp
RefSeq ORF	3768 bp
Locus ID	500261
UniProt ID	Q4L1J4
MW	136.3 kDa
Gene Summary	May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.