Pde6a (NM_001107386) Rat Tagged ORF Clone
CAT#: RR202734
- TrueORF®
Pde6a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), (10 ug)
"NM_001107386" in other vectors (3)
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CNY 10,930.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202734 representing NM_001107386
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGAGGTGACAGCAGAGGAGGTAGAAAAGTTCCTGGATTCAAATATTGGCTTTGCCAAACAATACT ATAATTTTCACTACCGGGGGAAGGTCGTCTCAGACCTCCTCGGGGCCAAGGAGGCAGCCGTGGACTTCAG CAACTACCACGATGTGAACAGGGTGGAGGAGAGTGAGATCATCTTTGACCTCCTGCGGGACTTTCAGGAG AACTTACAGGCCGAGAAATGCACGTTCAATGTCATGAAGAAGCTCTGCTTCCTCCTGCGGGCTGACCGCA TGAGCCTGTTCATGTACAGGACCCGCAATGGCATTGCAGAGCTAGCCACCAGGCTCTTCAACGTCCACAA GGATGCCGTCCTAGAGGACTGCTTGGTGATGCCAGACTCCGAGATTGTCTTCCCTTTGGACATGGGTGTC GTGGGCCACGTCGCACACTCCAAAAAGATCGCCAATGTCCCCAACACAGAAGAGGATGAGCATTTCTGCG ACTTCGTGGACAGCCTCACAGAATATCAGACCAAGAGCATCCTGGCATCCCCCATCATGAATGGGAAGGA CGTGGTAGCCATAATTATGGCTGTGAATAAAATAGATGAACCCCACTTCACCAAGAGAGATGAAGAGATT CTTCTCAAATACCTCAACTTTGTGAATCTGATCATGAAGGTATTCCACCTGAGTTACCTGCACAACTGTG AGACCCGGCGTGGCCAGATATTGCTGTGGTCTGGAAGCAAGGTCTTCGAAGAGCTCACGGATATAGAGAG GCAGTTCCACAAGGCCCTGTATACGGTCCGAGCTTTTCTCAACTGTGACAGATACTCTGTAGGACTCTTA GACATGACCAAACAGAAGGAATTTTTTGATGTGTGGCCAGTTCTGATGGGCGAGGCCCCAGCCTACTCTG GTCCCAGGACTCCAGATGGAAGGGAAATTAACTTTTACAAGGTCATTGACTACATCCTGCATGGCAAAGA AGACATCAAAGTAATCCCGAACCCACCTGCTGACCACTGGGCTCTAGTTAGCGGTCTTCCCACTTATGTG GCTCAAAATGGTCTGATCTGCAATATAATGAATGCGCCTGCAGAGGACTTTTTTGAATTCCAGAAAGAGC CTCTGGATGAATCTGGATGGATGATTAAAAACGTACTCTCCATGCCGATTGTCAACAAGAAGGAAGAGAT CGTGGGCGTGGCCACATTTTACAATCGCAAAGATGGGAAGCCCTTCGACGACATGGACGAGACCCTTATG GAGTCTTTGACTCAGTTTCTGGGCTGGTCAGTCTTAAACCCTGACACCTACGAGTCCATGAACAAACTTG AGAACAGGAAGGATATCTTCCAGGACATCGTAAAATATCACGTCAAGTGTGATAATGAGGAAATTCAGAA GATCTTGAAAACCAGAGAGGTGTATGGCAAAGAGCCGTGGGAATGCGAGGAGGAGGAGCTGGCTGAGATC CTGCAAGAAGAACTTCCAGATGCCGAGAAATATGAAATCAACAAGTTTCACTTCAGTGACCTGCCCCTTA CGGAACTGGAGCTGGTGAAATGCGGCATACAGATGTACTATGAGCTCAGAGTGGTGGACAAGTTCCACAT CCCGCAAGAGGCCCTGGTGCGCTTCATGTATTCGCTAAGCAAAGGCTACCGGAGGATCACTTACCACAAT TGGCGGCATGGCTTCAACGTGGGGCAGACCATGTTCTCCTTGCTGATGACAGGAAAGCTGAAGCGATACT TCACTGATTTGGAGGCCTTGGCCATGGTCACTGCAGCCTTCTGTCATGACATTGACCACAGAGGCACGAA CAACCTCTACCAGATGAAATCACAGAACCCCCTGGCCAAGCTTCATGGGTCCTCCATCCTGGAAAGGCAT CATTTGGAGTTTGGCAAAACATTGCTGAGAGATGAGAGCCTGAATATCTTTCAGAACCTGAATCGCCGGC AGCACGAGCATGCGATCCACATGATGGACATTGCGATCATCGCCACGGACCTTGCCCTGTATTTCAAGAA AAGGACAATGTTCCAGAAGATTGTGGATCAGTCAAAGACATATGAGAGTACCCAGGAGTGGACCCAGTAC ATGATGCTGGAGCAGACACGGAAGGAAATTGTGATGGCCATGATGATGACCGCCTGTGACCTCTCAGCCA TCACCAAGCCCTGGGAGGTGCAGAGCAAGGTGGCTCTGCTGGTGGCTGCTGAATTCTGGGAGCAAGGTGA CCTGGAGCGCACAGTGCTGCAACAGAATCCCATTCCCATGATGGACAGAAACAAGGCAGATGAACTCCCC AAGCTTCAGGTCGGCTTCATTGATTTTGTGTGCACCTTTGTCTATAAGGAATTCTCCCGGTTTCATGAGG AGATCACACCCATGTTGGATGGGATCACTAACAACCGTAAGGAGTGGAAGGCGCTGGCTGATGAGTACGA GGCCAGGATGAAAGCATTGGAGGAGGAGAAGCAGAAGCAGCAGGCAGCCAAGCAAGCTGCTTCCGGGAAC CAGCCAGGAGGGAACCCCAGTCCAGGGGGTGCACCTGCATCCAAGTCCTGTTGCATCCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202734 representing NM_001107386
Red=Cloning site Green=Tags(s) MGEVTAEEVEKFLDSNIGFAKQYYNFHYRGKVVSDLLGAKEAAVDFSNYHDVNRVEESEIIFDLLRDFQE NLQAEKCTFNVMKKLCFLLRADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDSEIVFPLDMGV VGHVAHSKKIANVPNTEEDEHFCDFVDSLTEYQTKSILASPIMNGKDVVAIIMAVNKIDEPHFTKRDEEI LLKYLNFVNLIMKVFHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLL DMTKQKEFFDVWPVLMGEAPAYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPADHWALVSGLPTYV AQNGLICNIMNAPAEDFFEFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDDMDETLM ESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEI LQEELPDAEKYEINKFHFSDLPLTELELVKCGIQMYYELRVVDKFHIPQEALVRFMYSLSKGYRRITYHN WRHGFNVGQTMFSLLMTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERH HLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESTQEWTQY MMLEQTRKEIVMAMMMTACDLSAITKPWEVQSKVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELP KLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYEARMKALEEEKQKQQAAKQAASGN QPGGNPSPGGAPASKSCCIQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001107386 |
ORF Size | 2580 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001107386.1, NP_001100856.1 |
RefSeq Size | 4104 bp |
RefSeq ORF | 2583 bp |
Locus ID | 307401 |
MW | 99.8 kDa |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202734 | Pde6a (untagged ORF) - Rat phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), (10 ug) |
CNY 10,930.00 |
|
RR202734L3 | Lenti ORF clone of Pde6a (Myc-DDK-tagged ORF) - Rat phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), (10 ug) |
CNY 12,830.00 |
|
RR202734L4 | Lenti ORF clone of Pde6a (mGFP-tagged ORF) - Rat phosphodiesterase 6A, cGMP-specific, rod, alpha (Pde6a), (10 ug) |
CNY 13,970.00 |