Gpt (NM_031039) Rat Tagged ORF Clone
CAT#: RR202833
- TrueORF®
Gpt (Myc-DDK-tagged ORF) - Rat glutamic-pyruvate transaminase (alanine aminotransferase) (Gpt), (10 ug)
"NM_031039" in other vectors (3)
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CNY 6,370.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | ALAT; Gpt1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR202833 representing NM_031039
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCACGGGTGAATGATCAAAGCCAGGCTTCAAGGAATGGGCTGAAGGGAAAGGTGCTAACTCTGG ACACTATGAACCCATGTGTGCGGAGGGTGGAGTATGCAGTTCGAGGACCCATTGTGCAGCGTGCCTTGGA GCTGGAGCAGGAGCTGCGTCAGGGTGTGAAGAAGCCGTTTACTGAGGTCATCCGTGCCAACATTGGGGAT GCACAAGCCATGGGGCAGAGACCCATCACCTTCTTCCGCCAGGTCCTGGCCCTCTGTGTCTACCCCAATC TTCTGAGCAGTCCTGACTTCCCAGAGGATGCCAAGAGAAGGGCAGAACGCATCTTGCAGGCCTGCGGGGG CCACAGCCTGGGTGCCTATAGCATTAGCTCTGGAATCCAGCCGATCCGGGAGGATGTGGCGCAATACATT GAGAGAAGAGACGGAGGCATCCCCGCAGACCCGAACAACATATTTCTATCCACAGGGGCCAGCGATGCCA TCGTGACAATGCTCAAGCTGCTGGTATCTGGCGAGGGCCGTGCACGAACAGGTGTACTCATTCCCATTCC TCAGTACCCACTGTACTCAGCCGCGCTGGCTGAACTGGACGCCGTGCAAGTGGACTACTACCTGGACGAA GAGCGCGCCTGGGCTCTGGACATCGCAGAGCTGCGGCGCGCTCTGTGCCAGGCACGTGACCGTTGCTGCC CTCGAGTACTGTGCGTCATCAACCCCGGCAACCCCACTGGGCAGGTGCAGACCCGTGAGTGCATCGAGGC CGTAATCCGCTTTGCTTTCAAAGAAGGACTCTTCTTGATGGCTGATGAGGTATACCAGGACAACGTGTAT GCCGAGGGCTCTCAGTTCCATTCATTCAAGAAGGTGCTCATGGAGATGGGGCCACCGTATTCCACGCAGC AGGAGCTTGCTTCTTTCCACTCAGTCTCTAAGGGCTACATGGGCGAGTGCGGGTTTCGTGGTGGCTATGT GGAGGTGGTAAACATGGATGCTGAGGTGCAGAAACAGATGGGGAAGCTGATGAGTGTGCGGCTGTGTCCA CCAGTGCCAGGCCAGGCCTTGATGGACATGGTGGTCAGTCCGCCAACACCCTCCGAGCCGTCCTTCAAGC AGTTTCAAGCAGAGAGACAGGAGGTGCTGGCTGAACTGGCAGCCAAGGCTAAGCTCACGGAGCAGGTCTT CAATGAGGCTCCCGGGATCCGCTGCAACCCAGTGCAGGGCGCCATGTATTCCTTCCCTCAAGTGCAGCTG CCCTTGAAAGCGGTGCAGCGTGCTCAGGAACTGGGCCTGGCCCCTGACATGTTCTTCTGCCTGTGCCTCC TGGAAGAGACTGGCATCTGCGTTGTGCCCGGGAGTGGCTTTGGGCAGCAGGAGGGCACCTATCATTTCCG GATGACCATTCTGCCCCCCATGGAGAAACTGCGGCTGCTGCTGGAAAAACTCAGTCACTTCCATGCCAAG TTCACCCATGAGTACTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202833 representing NM_031039
Red=Cloning site Green=Tags(s) MASRVNDQSQASRNGLKGKVLTLDTMNPCVRRVEYAVRGPIVQRALELEQELRQGVKKPFTEVIRANIGD AQAMGQRPITFFRQVLALCVYPNLLSSPDFPEDAKRRAERILQACGGHSLGAYSISSGIQPIREDVAQYI ERRDGGIPADPNNIFLSTGASDAIVTMLKLLVSGEGRARTGVLIPIPQYPLYSAALAELDAVQVDYYLDE ERAWALDIAELRRALCQARDRCCPRVLCVINPGNPTGQVQTRECIEAVIRFAFKEGLFLMADEVYQDNVY AEGSQFHSFKKVLMEMGPPYSTQQELASFHSVSKGYMGECGFRGGYVEVVNMDAEVQKQMGKLMSVRLCP PVPGQALMDMVVSPPTPSEPSFKQFQAERQEVLAELAAKAKLTEQVFNEAPGIRCNPVQGAMYSFPQVQL PLKAVQRAQELGLAPDMFFCLCLLEETGICVVPGSGFGQQEGTYHFRMTILPPMEKLRLLLEKLSHFHAK FTHEYS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031039 |
ORF Size | 1488 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031039.1, NP_112301.1 |
RefSeq Size | 1744 bp |
RefSeq ORF | 1491 bp |
Locus ID | 81670 |
UniProt ID | P25409 |
MW | 55.1 kDa |
Gene Summary | human homolog is a cytosolic alanine aminotransferase [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN202833 | Gpt (untagged ORF) - Rat glutamic-pyruvate transaminase (alanine aminotransferase) (Gpt), (10 ug) |
CNY 6,370.00 |
|
RR202833L3 | Lenti ORF clone of Gpt (Myc-DDK-tagged ORF) - Rat glutamic-pyruvate transaminase (alanine aminotransferase) (Gpt), (10 ug) |
CNY 8,270.00 |
|
RR202833L4 | Lenti ORF clone of Gpt (mGFP-tagged ORF) - Rat glutamic-pyruvate transaminase (alanine aminotransferase) (Gpt), (10 ug) |
CNY 9,030.00 |