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Gtf2i (NM_001001512) Rat Tagged ORF Clone
CAT＃: RR202933
- TrueORF®
Gtf2i (Myc-DDK-tagged ORF) - Rat general transcription factor II I (Gtf2i), (10 ug)
"NM_001001512" in other vectors (3)
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CNY 11,780.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gtf2ird1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR202933 representing NM_001001512
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGTAGCGATGTCCGCGCTGCCTGCGGAGGATGAGGAGTCTTCGGAGAGCAGGATGGTGGTGA CGTTCCTCATGTCTGCCCTGGAGTCCATGTGTAAAGAGCTGGCCAAGTCCAAGGCTGAGGTGGCCTGCAT CGCCGTGTATGAGACAGATGTGTTTGTCGTTGGAACCGAAAGAGGGCGTGCTTTTGTCAATACCAGAAAA GATTTCCAAAAAGATTTTGTGAAGTATTGTGTTGAAGAAGAAGAAAAGGCCGCCGAGATGCATAAGATGA AATGTACAACCCAGGCCAACCGGATGAGCGTGGATGCTGTAGAAATTGAGACCCTCAGAAAAACGGTGGA GGACTATTTCTGCTTTTGTTATGGGAAAGCCTTAGGCAAATCCACAGTGGTCCCTGTGCCATATGAGAAG ATGCTGCGTGACCAGTCAGCTGTGGCGGTGCAGGGGCTTCCCGAGGGGGTCGCCTTCAAGCATCCCGAGC ACTATGACCTCGCAACCTTGAAGTGGATTCTGGAGAACAAGGCGGGGATTTCCTTCATCATCAAGAGACC TTTCCTCGAGCCGAAGAAACACCTTGGTGGTCGAGTGATGGCGGCCGATGCTGACAGGCCCATGCTGTCT CCGGGTGGAAGTTGTGGCCCCATCAAAGTGAAAACTGAGCCCACGGAAGATTCTGATGATGATTATTCTC CACCTACCAAGAGGCCAAAGAGCAGTGAGCCGCCCCCACCCCCACCGGTCCCAGAGCCCACCAACGCCGG CAAGCGCAAAGTGAGGGAGTTCAACTTTGAGAAATGGAACGCGCGCATCACTGACCTACGGAAACAAGTG GAAGAATTGTTTGAAAGAAAATACGCTCAAGCTATAAAAGCCAAAGGCCCGGTGACGATCCCATACCCTC TTTTCCAGTCGCACGTCGAAGATCTGTATGTAGAGGGCCTTCCTGAAGGGATCCCCTTTAGAAGGCCATC AACGTACGGCATTCCTCGCCTGGAGAGGATATTGCTGGCAAAGGAGAGGATCCGCTTTGTGATCAAGAAA CATGAGCTTCTGAACTCAACACGAGAAGATTTACAGCTGGACAAGCCCGCATCAGGAGTGAAGGAAGAGT GGTATGCTCGTATCACCAAACTGAGGAAGATGGTTGACCAGCTCTTCTGCAAGAAATTTGCTGAGGCCTT GGGAAGCACAGAAGCCAAGGCTGTACCGTACCAAAAATTCGAGGCCTATCCCAATGATCTCTATGTGGAA GGACTTCCAGAAAACATTCCTTTCCGAAGCCCCTCATGGTATGGAATCCCAAGACTGGAAAAAATCATCC AAGTGGGCAATCGAATTAAATTTGTTATCAAGAGACCAGAACTGCTGACTCACAGTACAACTGAGGTGAC TCAGCCACGGACGAATACGCCAGTCAAGGAAGATTGGAACGTCAGGATCACCAAGCTCCGCAAGCAGGTG GAGGAGATCTTTAACTTGAAGTTTGCTCAAGCTCTTGGCCTCACAGAGGCTGTGAAGGTGCCGTATCCTG TGTTCGAATCCAACCCAGAATTTCTGTATGTGGAAGGGCTCCCTGAGGGGATCCCCTTCCGAAGCCCTAC CTGGTTTGGGATTCCACGCCTTGAGAGGATTGTCCGTGGGAGCAATAAGATCAAGTTTGTTGTTAAAAAG CCCGAGCTAGTTGTGTCCTACTTGCCTCCTGGGATGGCTAGCAAAATCAACACTAAAGCATTGCAGTCCC CGAAACGACCACGAAGCCCTGGGAGCAACTCCAAGGTCCCTGAAATCGAGGTCACTGTGGAAGGCCCCAA CAACAACAGTCCTCAGACCTCTACCGTTCGAACTCCTACCCAGACCAACGGTTCAAACGTTCCCTTCAAG CCTCGAGGGAGAGAGTTTTCCTTTGAGGCCTGGAACGCTAAGATCACAGACCTGAAGCAGAAAGTGGAGA ATCTCTTCAATGAAAAGTGCGGGGAAGCTCTTGGCCTTAAGCAGGCTGTGAAAGTACCGTTTGCGCTATT TGAGTCCTTCCCAGAGGACTTCTACGTAGAAGGCTTACCCGAGGGCGTGCCCTTCCGAAGACCATCCACT TTTGGCATTCCAAGGCTGGAGAAGATCCTCCGGAACAAGGCCAAGATAAAGTTCATCATTAAAAAACCTG AGATGTTCGAGACGGCCATCAAAGAGAGCACCTCTTCCAAAAGCCCTCCGAGGAAGACGAACTCATCCCC CAGTGTCAATACGACAGCCTCAGGTGTGGAAGATCTTAACATCATTCAGGTGACAATCCCAGATGACGAC AACGAACGACTCTCCAAAGTCGAGAAGGCCAGGCAGCTTCGGGAGCAAGTCAACGACCTCTTCAGCCGAA AGTTTGGTGAAGCTATTGGGATGGGCTTCCCCGTGAAGGTCCCCTACAGGAAAATCACCATCAACCCTGG CTGCGTGGTGGTCGATGGCATGCCCCCGGGGGTGTCCTTCAAAGCCCCCAGCTATCTGGAGATCAGCTCC ATGAGAAGGATCTTAGATTCTGCTGAGTTTATCAAGTTCACTGTCATTAGACCATTCCCAGGACTGGTGA TTAATAACCAGCTGGTTGATCAGAATGAGTCTGAAGGCCCTGTGATACAAGAATCAGCTGAGGCAAGCCA GTTGGAAGTTCCAGCAACAGAAGAAATCAAAGAGACAGATGGGAGCTCACAGATCAAGCAGGAGCCAGAC CCCACGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR202933 representing NM_001001512
Red=Cloning site Green=Tags(s) MAQVAMSALPAEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVYETDVFVVGTERGRAFVNTRK DFQKDFVKYCVEEEEKAAEMHKMKCTTQANRMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEK MLRDQSAVAVQGLPEGVAFKHPEHYDLATLKWILENKAGISFIIKRPFLEPKKHLGGRVMAADADRPMLS PGGSCGPIKVKTEPTEDSDDDYSPPTKRPKSSEPPPPPPVPEPTNAGKRKVREFNFEKWNARITDLRKQV EELFERKYAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERILLAKERIRFVIKK HELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQLFCKKFAEALGSTEAKAVPYQKFEAYPNDLYVE GLPENIPFRSPSWYGIPRLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLRKQV EEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPTWFGIPRLERIVRGSNKIKFVVKK PELVVSYLPPGMASKINTKALQSPKRPRSPGSNSKVPEIEVTVEGPNNNSPQTSTVRTPTQTNGSNVPFK PRGREFSFEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVEGLPEGVPFRRPST FGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSKSPPRKTNSSPSVNTTASGVEDLNIIQVTIPDDD NERLSKVEKARQLREQVNDLFSRKFGEAIGMGFPVKVPYRKITINPGCVVVDGMPPGVSFKAPSYLEISS MRRILDSAEFIKFTVIRPFPGLVINNQLVDQNESEGPVIQESAEASQLEVPATEEIKETDGSSQIKQEPD PTW TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001512 |
| ORF Size | 2739 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001512.2, NP_001001512.2 |
| RefSeq Size | 3988 bp |
| RefSeq ORF | 2742 bp |
| Locus ID | 353256 |
| UniProt ID | Q5U2Y1 |
| MW | 103 kDa |
| Gene Summary | Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box (By similarity). Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN202933 | Gtf2i (untagged ORF) - Rat general transcription factor II I (Gtf2i), (10 ug) |
CNY 11,780.00 |
|
| RR202933L3 | Lenti ORF clone of Gtf2i (Myc-DDK-tagged ORF) - Rat general transcription factor II I (Gtf2i), (10 ug) |
CNY 13,490.00 |
|
| RR202933L4 | Lenti ORF clone of Gtf2i (mGFP-tagged ORF) - Rat general transcription factor II I (Gtf2i), (10 ug) |
CNY 14,820.00 |
