Abca7 (NM_207598) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203110 representing NM_207598 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCTGCACACAGTTGATGCTCCTACTGTGGAAAAATTACACATATCGACGGAGACAACCGATCC AACTAGTAGTGGAGTTACTGTGGCCCCTCTTCCTCTTCTTCATCCTAGTGGCCGTCCGTCACTCACACCC CCCACTGGAGCACCACGAATGCCACTTTCCAAACAAGCCACTACCATCAGCGGGCACGGTGCCCTGGCTG CAGGGCCTTGTCTGCAACGTAAATAACTCCTGCTTCCAACACCCAACGCCTGGAGAGAAGCCTGGCGTCC TGAGCAACTTTAAGGATTCCTTGATCTCGAGGCTCCTTGCCGATGCTCACACAGTGCTGGGGGGCCACAG CACCCAGGACATGCTGGCTGCCCTGGGGAAACTGATCCCCGTGCTCAGGGCTGTTGGGAGCGGAGCCTGG CCACAGGAGAGTAACCAACCGGCTAAGCAAGGGTCAGTGACTGAGCTCTTGGAGAAGATCCTGCAACGGG CATCCCTGGAGACCGTGCTGGGTCAAGCCCAGGATTCCATGAGAAAGTTCTCAGATGCTACCAGAACTGT TGCCCAGGAGCTCCTGACGCTACCCAGCCTTGTGGAACTCCGGGCTTTGCTGCGGAGGCCCCGAGGGTCA GCTGGCTCTCTGGAGCTGATTTCGGAGGCCCTCTGCAGTACCAAGGGGCCCAGCAGTCCAGGGGGTCTGT CCCTCAACTGGTACGAAGCCAACCAGATCAACGAATTCATGGGGCCTGAGCTGGCGCCCACCCTGCCTGA CAGCAGTCTCAGCCCCGCCTGCTCTGAGTTCGTGGGGGCACTGGATGACCACCCTGTGTCTCGGCTGCTC TGGAGACGCCTGAAGCCATTGATCCTCGGGAAAATTCTCTTTGCACCTGACACAAACTTCACTAGGAAAC TCATGGCTCAGGTGAACCAGACCTTTGAGGAGCTGGCTCTGCTGAGGGACCTACACGAACTCTGGGGAGT ACTGGGACCCCAGATCTTCAACTTCATGAATGACAGTACAAACGTGGCCATGCTTCAGAAACTGCTGGAT GTGGAGGGCACAGGGTGGCAGCAGCAGACACCCAAAGGCCAGAAGCAGTTGGAGGCTATCAGAGACTTTC TGGATCCTAGTAGGGGTCGATACAACTGGCAGGAGGCTCATGCAGACATGGGGCGCCTGGCAGAAATACT GGGCCAAATCTTGGAGTGTGTGTCCCTGGACAAGCTGGAGGCTGTGCCCTCAGAGGAAGCTCTTGTGTCC CGTGCCCTGGAGCTGCTGGGTGAGCGCCGCCTCTGGGCAGGCATCGTCTTCCTGAGCCCAGAGCACCCTC TGGACTCATCTGAACCGCCATCTCCAACCACCACGGGTCCTGGCCACCTACGAGTCAAGATTCGAATGGA TATTGATGATGTCACAAGGACCAATAAGATCAGGGACAAGTTTTGGGACCCAGGTCCGTCAGCAGATCCC CTCATGGACCTTCGGTATGTGTGGGGCGGCTTCGTGTACCTGCAGGACCTGCTGGAGCAGGCAGCTGTGC GCGTGCTGAGTGGCAGGGACTCCAGAGCAGGCCTCTATTTGCAGCAGATGCCACACCCCTGCTACGTGGA CGATGTGTTCCTGCGGGTGCTGAGCCGGTCTCTGCCTCTGTTTCTGACTCTGGCCTGGATCTACTCGGTG GCGCTCACTGTGAAGGCCGTGGTGCGCGAGAAAGAGACACGGCTGCGAGAAACCATGCGTGCGATGGGGT TGAGCCGTGCTGTGCTCTGGCTTGGTTGGTTCCTCAGCTGCCTGGGACCCTTCTTGGTCAGCGCTGCATT GTTGGTGTTAGTGCTTAAGCTAGGGAACATCCTTCCTTACAGCCACCCGGTTGTAGTCTTCCTTTTCTTG GCGGCCTTTGCGGTGGCCACCGTCGCTCAGAGTTTTCTGCTCAGTGCCTTCTTCTCCCGGGCCAACCTGG CAGCAGCCTGCGGGGGCCTAGCCTACTTCGCGCTCTACCTGCCCTATGTGCTGTGTGTCGCCTGGCGTGA GCGCCTGCCTCTGGGCGGACTCTTAGCTGTGAGCCTGCTGTCCCCCGTGGCCTTTGGCTTTGGATGCGAG AGCCTGGCTCTACTGGAGGAGCAGGGGGACGGGGCTCAGTGGCACAATTTGGGCACAGGCCCGGCGGAGG ATGTCTTCAGCCTGGCACAGGTGTCTGCCTTCCTGTTGCTTGATGCTGTCATCTACGGCCTTGCCCTCTG GTACCTAGAGGCTGTATGCCCAGGCCAGTATGGAATCCCTGAACCATGGAATTTCCCCTTTCGGAGGAGC TACTGGTGTGGCCCTGGACCTCCCAAGAGTTCTGTCTTGGCCCCTGCCCCACAAGATCCCAAGGTTCTGG TGGAAGAGCCACCACCTGGTCTGGTTCCTGGTGTCTCCATTCGAGGCCTGAAGAAGCATTTTCGTGGCTC TCCACAGCCAGCCCTGCGAGGGCTCAACCTTGACTTCTATGAAGGCCACATCACTGCCTTTTTGGGTCAC AACGGGGCTGGCAAGACAACCACACTGTCCATCTTGAGTGGTCTCTTCCCACCCAGTAGTGGCTCGGCCT CCATCCTGGGCCATGACGTGCAAACCAACATGGCAGCCATCCGGCCCCACCTGGGCATCTGCCCACAGTA CAATGTGCTGTTTGACATGCTGACGGTGGAAGAACACGTTTGGTTCTATGGCCGTTTGAAAGGCGTGAGT GCAGCCGCCATAGACTCCGAGCAGGAACATTTGATTCGGGATGTGGGGCTCATCCCTAAGCGGGACACAC AGACGCGTCACCTCTCTGGAGGAATGCAGCGAAAACTTTCTGTGGCTATTGCCTTTGTGGGTGGCTCTCG TGTGGTTATCATGGACGAGCCCACTGCTGGTGTGGACCCCGCTTCCCGCCGTGGCATTTGGGAATTGCTA CTCAAGTACAGAGAAGGTCGGACACTGATCCTCTCCACTCACCACCTGGATGAGGCAGAGCTCCTGGGAG ACCGTGTGGCCATGGTGGCAAGTGGCTCTTTGTGCTGCTGTGGGTCCCCACTTTTCTTGCGCCGACACTT GGGCTGCGGTTACTACCTGACGCTGGTGAAGAGTTCTCAGTCCCTTGTCACCCATGACTTGAAGGGAGAC ACTGAGGACCCCAGACGGGAAAAGAAGTCAGGCAGCGAGGGGAAGACGGCAGACACAGTGCTCACACGAG ACGGCCCTCACAGGAGCAGCCAGGTCCCAGCTCCTGATGCAGTCCCTGTCACCCCAAGCGCAGCCCTGAT CCTAGAGCTAGTGCAGCGTCACGTGCCCGGAGCGCAGCTGGTAGAGGAACTGCCCCATGAGCTGGTGCTT GCACTGCCCTATGCGGGTGCCCTGGATGGCAGCTTTGCCACAGTCTTCCAAGAGCTGGACCAGCAGCTGG AGCGCCTGGGGCTCACTGGCTACGGGATCTCAGACACCAACCTGGAGGAGATCTTCCTGAAGGTGGTGGA GGAAGCACACGCACACGGAGAAGGTGGTGACCCCAGACAGCAGCAGCACCTTCTCACAGCCACTCCGCAG CCCCACACAGGGCCAGAGGCATCGGTTCTGGAGAATGGGGAGCTGGCTAAGCTGGTGCTGGACCCCCAAG CCCCAAAAGGCTCTGCACCCACCACTGCCCAAGTGCAAGGCTGGACACTCACCTGCCAACAGCTCCGGGC TTTGCTCCACAAGCGTTTTCTGCTCGCTCGCCGCAGCCGCCGGGGCCTGTTTGCACAGATCGTGCTGCCC GCCCTCTTTGTGGGCCTGGCCCTGTTTTTCACCCTCATCGTGCCTCCCTTTGGCCAGTACCCACCCCTGC AGCTCAGCCCTGCTATGTATGGCCCTCAGGTCTCCTTCTTCAGTGAGGATGCCCCTGCCGACCCCAACCG GATGAAGCTGCTGGAGGCTCTGCTAGGGGAGGCCGGGCTGCAGGACCCGAGCGTGCAGGGCAAAGGTTCC AGGGGATCTGAGTGTACACATTCCCTGGCTTGCTACTTCACGGTCCCAGAGGTCCCTCCAGATGTGGCCA GCATTCTGGCTAGCGGCAACTGGACTCCAGACTCTCCATCTCCAGCCTGCCAGTGCAGCCAGCCTGGAGC CCGCCGCTTGCTGCCAGATTGCCCTGCTGGAGCTGGGGGTCCACCACCACCCCAGGCTATGGCTGGCTTT GGGGAGGTGGTCCAGAATCTCACTGGCCGAAATGTGTCTGACTTTCTGGTGAAGACATACCCCAGCCTGG TGCGCCGGGGCCTGAAGACCAAGAAGTGGGTGGATGAGGTCAGATACGGGGGCTTCTCCCTGGGAGGCCG AGATCCAGACCTGCCCTCAGGGCGTGAGGTGGTCCGCACAGTGGCAGAGATGCGGGCACTGCTGAGCCCT CAACCTGGCAATACACTAGATCGTATCCTGAACAACCTCACTCAGTGGGCCCTTGGTCTTGACGCCCGGA ACAGCCTCAAGATCTGGTTCAACAACAAGGGCTGGCATGCCATGGTGGCCTTTGTGAACCGAGCCAACAA TGGACTCCTACGTGCCTTCCTACCATCTGGTTCAGTCCGCCATGCCCACAGCATCACTACACTCAACCAT CCTTTGAACTTGACCAAGGAGCAGCTGTCTGAAGCTACGCTGATAGCCTCCTCTGTGGATGTTCTTGTCT CTATCTGTGTGGTCTTCGCCATGTCATTTGTCCCAGCCAGCTTTACCCTAGTCCTCATAGAAGAACGCAT CACCAGAGCCAAGCATCTGCAGCTGGTCAGTGGCCTGCCCCAAACCCTCTACTGGCTTGGCAACTTCCTC TGGGACATGTGTAACTACTTGGTGGCAGTGTGCATAGTGGTGCTCATCTTCCTGGCCTTTCAGCAGAAAG CCTATGTAGCTCCAGAGAACCTGCCTGCTCTCTTACTCTTGTTACTGCTGTATGGCTGGTCTATTACGCC ACTCATGTACCCAGCCTCCTTCTTCTTCTCCGTGCCCAGCACGGCCTATGTGGTGCTCACCTGCATCAAC CTCTTCATCGGCATCAACAGCAGCATGGCCACTTTCGTGCTGGAACTGCTTTCAGATCAGAACCTTCAAG AAGTGAGCCGGATCCTGAAGCAAGTGTTTCTTATCTTCCCCCACTTCTGCCTTGGCCGAGGGCTCATTGA CATGGTGCGGAACCAGGCCATGGCAGATGCCTTTGAGCGCTTAGGAGACAAGCAATTCCAGTCGCCCCTA CGCTGGGACATCATTGGCAAGAACCTCCTGGCCATGGTGGCCCAGGGTCCCCTGTTCCTCCTAATCACAC TCCTGCTCCAACATCGCAACCGCCTCCTGCCACAACCAAAATCAAGACTGCCGCCGCCCCTGGGGGAGGA GGATGAGGATGTGGTTCGAGAGCGTGAGCGGGTGACCAAGGGAGCCACCCAGGGGGATGTGCTGGTCCTC AGGGACTTGACCAAGGTGTACCGTGGGCAGAGGAGCCCAGCAGTGGATCACCTGTGCTTGGGGATCCCCC CTGGGGAGTGTTTCGGGCTGCTGGGTGTCAATGGGGCAGGGAAGACATCCACCTTCCGCATGGTGACGGG GGACACGCTGCCCAGCAGTGGTGAAGCAGTGCTGGCAGGCCACAATGTGGCCCAGGAGCCGTCTGCTGCG CACCGCAGCATGGGCTACTGTCCCCAGTCTGATGCCATCTTCGATCTGCTGACCGGCCGGGAACATCTAG AACTGTTTGCGCGCCTGCGCGGGGTGCCCGAGGCCCAAGTTGCCCAGACAGCGCTCTCTGGCCTGGTGCG CCTGGGCCTTCCTAGCTACGCAGACCGACCCGCGGGTACCTACAGCGGAGGCAACAAACGGAAGCTGGCG ACAGCTCTGGCTCTGGTTGGTGATCCAGCAGTGGTCTTTCTGGACGAGCCCACCACAGGCATGGACCCAA GTGCGCGGCGATTTCTTTGGAACAACTTGCTGTCCGTGGTGCGTGAGGGCCGCTCCGTAGTGCTCACGTC GCACAGCATGGAAGAGTGTGAAGCGCTCTGCACGCGCCTGGCCATCATGGTGAACGGGCGGTTCCGCTGT CTGGGAAGCGCTCAGCATCTCAAAAGCAGATTCGGGGCTGGCCACACACTGACTCTCAGGGTCCCACCGG ACCAGCCGGAACCGGCGATAGCCTTCATTGTGACCACATTCCCTGATGCTGAGCTTCGGGAGGTGCATGG CAGCCGTCTGCGCTTTCAGCTGCCGCCTGGGGGCGGATGCACGCTGGCACGAGTGTTCAGGGAGCTGGCT GCGCAAGGCAAGGCCCACGGTGTGGAGGACTTCTCTGTGAGCCAGACCACTTTGGAGGAGGTGTTCCTAT ATTTCTCCAAAGACCAGGGGGAAGAGGAGGAAGGCAGTGGACAGGAGACTGAAACGAGGGAAGTTTCCAC ACCTGGCCTGCAGCATCCCAAACGGGTCAGCCGATTCCTGGAAGACCCCAGCTCTGTGGAGACCGTGATC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203110 representing NM_207598 Red=Cloning site Green=Tags(s) MAFCTQLMLLLWKNYTYRRRQPIQLVVELLWPLFLFFILVAVRHSHPPLEHHECHFPNKPLPSAGTVPWL QGLVCNVNNSCFQHPTPGEKPGVLSNFKDSLISRLLADAHTVLGGHSTQDMLAALGKLIPVLRAVGSGAW PQESNQPAKQGSVTELLEKILQRASLETVLGQAQDSMRKFSDATRTVAQELLTLPSLVELRALLRRPRGS AGSLELISEALCSTKGPSSPGGLSLNWYEANQINEFMGPELAPTLPDSSLSPACSEFVGALDDHPVSRLL WRRLKPLILGKILFAPDTNFTRKLMAQVNQTFEELALLRDLHELWGVLGPQIFNFMNDSTNVAMLQKLLD VEGTGWQQQTPKGQKQLEAIRDFLDPSRGRYNWQEAHADMGRLAEILGQILECVSLDKLEAVPSEEALVS RALELLGERRLWAGIVFLSPEHPLDSSEPPSPTTTGPGHLRVKIRMDIDDVTRTNKIRDKFWDPGPSADP LMDLRYVWGGFVYLQDLLEQAAVRVLSGRDSRAGLYLQQMPHPCYVDDVFLRVLSRSLPLFLTLAWIYSV ALTVKAVVREKETRLRETMRAMGLSRAVLWLGWFLSCLGPFLVSAALLVLVLKLGNILPYSHPVVVFLFL AAFAVATVAQSFLLSAFFSRANLAAACGGLAYFALYLPYVLCVAWRERLPLGGLLAVSLLSPVAFGFGCE SLALLEEQGDGAQWHNLGTGPAEDVFSLAQVSAFLLLDAVIYGLALWYLEAVCPGQYGIPEPWNFPFRRS YWCGPGPPKSSVLAPAPQDPKVLVEEPPPGLVPGVSIRGLKKHFRGSPQPALRGLNLDFYEGHITAFLGH NGAGKTTTLSILSGLFPPSSGSASILGHDVQTNMAAIRPHLGICPQYNVLFDMLTVEEHVWFYGRLKGVS AAAIDSEQEHLIRDVGLIPKRDTQTRHLSGGMQRKLSVAIAFVGGSRVVIMDEPTAGVDPASRRGIWELL LKYREGRTLILSTHHLDEAELLGDRVAMVASGSLCCCGSPLFLRRHLGCGYYLTLVKSSQSLVTHDLKGD TEDPRREKKSGSEGKTADTVLTRDGPHRSSQVPAPDAVPVTPSAALILELVQRHVPGAQLVEELPHELVL ALPYAGALDGSFATVFQELDQQLERLGLTGYGISDTNLEEIFLKVVEEAHAHGEGGDPRQQQHLLTATPQ PHTGPEASVLENGELAKLVLDPQAPKGSAPTTAQVQGWTLTCQQLRALLHKRFLLARRSRRGLFAQIVLP ALFVGLALFFTLIVPPFGQYPPLQLSPAMYGPQVSFFSEDAPADPNRMKLLEALLGEAGLQDPSVQGKGS RGSECTHSLACYFTVPEVPPDVASILASGNWTPDSPSPACQCSQPGARRLLPDCPAGAGGPPPPQAMAGF GEVVQNLTGRNVSDFLVKTYPSLVRRGLKTKKWVDEVRYGGFSLGGRDPDLPSGREVVRTVAEMRALLSP QPGNTLDRILNNLTQWALGLDARNSLKIWFNNKGWHAMVAFVNRANNGLLRAFLPSGSVRHAHSITTLNH PLNLTKEQLSEATLIASSVDVLVSICVVFAMSFVPASFTLVLIEERITRAKHLQLVSGLPQTLYWLGNFL WDMCNYLVAVCIVVLIFLAFQQKAYVAPENLPALLLLLLLYGWSITPLMYPASFFFSVPSTAYVVLTCIN LFIGINSSMATFVLELLSDQNLQEVSRILKQVFLIFPHFCLGRGLIDMVRNQAMADAFERLGDKQFQSPL RWDIIGKNLLAMVAQGPLFLLITLLLQHRNRLLPQPKSRLPPPLGEEDEDVVRERERVTKGATQGDVLVL RDLTKVYRGQRSPAVDHLCLGIPPGECFGLLGVNGAGKTSTFRMVTGDTLPSSGEAVLAGHNVAQEPSAA HRSMGYCPQSDAIFDLLTGREHLELFARLRGVPEAQVAQTALSGLVRLGLPSYADRPAGTYSGGNKRKLA TALALVGDPAVVFLDEPTTGMDPSARRFLWNNLLSVVREGRSVVLTSHSMEECEALCTRLAIMVNGRFRC LGSAQHLKSRFGAGHTLTLRVPPDQPEPAIAFIVTTFPDAELREVHGSRLRFQLPPGGGCTLARVFRELA AQGKAHGVEDFSVSQTTLEEVFLYFSKDQGEEEEGSGQETETREVSTPGLQHPKRVSRFLEDPSSVETVI SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_207598
ORF Size	6510 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_207598.1, NP_997481.1
RefSeq Size	6513 bp
RefSeq ORF	6513 bp
Locus ID	299609
UniProt ID	Q7TNJ2
MW	237.7 kDa
Gene Summary	may play a role in platelet function [RGD, Feb 2006]

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