Elp1 (NM_080899) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Ikbkap
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203572 representing NM_080899 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAAATCTGAAGTTGCATCAGACCCTGGAATTTAGGGACATTCAGGCACCAGGGAAACCTCAGTGTT TCTGTCTGCGAGCTGAACAGGGGACAGTTCTGATTGGCTCAGAACGTGGACTGACAGAAGTAGATCCTGT GAAAAAAGAAGTGAAGACTGAAATTTCTTTGGTGGCAGAAGGTTTTCTTCCAGAGGATGGAAGTGGCTGC ATTGTGGGTATTCAGGACTTGCTGGATCAAGAATCTGTGTGTGTGGCCACTGCCTCTGGGGACGTCATAG TCTGCAATGTCAGCACACAGCAGCTGGAATGTGTTGGGAATGTGGCCAGTGGTATCTCTGTCATGAGCTG GAGTCCTGATCAAGAACTGCTGCTGCTTGCCACAGCTCAACAGACCCTGATTATGATGACAAGAGACTAT GAAGTAATCACAGAGCAGCAGATCCACCAGGATGATTTTGGAGAAGGCAAGTTTATCACCGTTGGATGGG GCAGTAAGGACACGCAGTTCCACGGCTCAGAAGGCAGGCCAATAACCTTTCCGGTGCAAATGCATGAGTC TGCCTTGTCCTGGGATGACCACAGACCACAAATTACCTGGCGTGGGGATGGGCAATTTTTTGCTGTGAGT GTTGTTTGCTCACAAACAGGGGCTCGGAAAATCCGAGTATGGAACCGAGAGTTTGCCTTGCAGTCAACCA GTGAGTCTGTGCCAGGGCTGGGGCCCTCCCTGGCTTGGAAACCCTCAGGCAGTCTGATTGCATCTACTCA GGATAAACCCAACCAGCAAGACGTTGTGTTTTTTGAGAAAAATGGACTTCTTCATGGGTATTTTACACTT CCTTTCTTGAAAGATGAAGTTAAGGTAAATGACTTGCTGTGGAATGCAGATTCCTCGGTGCTTGCTGTGT GGCTAGAAGACCTCCCGAAGGAAGGCAGTTCCACTCTGAAAAGCTATGTCCAGCTCTGGACCGTGGGGAA CTATCACTGGTATCTCAAGCAAAGCCTGCCCTTCAGCACCACGGGGAAGAACCAGATCGTGTCTCTGCTG TGGGACCCAGTGACGCCGGGCCGACTGCATGTTCTCTGTCAGGGCTGGCGGTATCTATGCTGTGACTGGC ACTGGACCACTGATCGGAGCTCTGGAAATAGTGCCGATGACCTGGCAAATGTGGCTGTCATTGATGGAAA CAAGGTGTTGGTAACAGTCTTCCAGCGGACTGTGACCCCACCTCCCATGTGTACCTACCGGCTGCTGATC CCACATCCAGTGAATCAAGTCATGTCCTCTGCTCACCTTGGAAATGACCTTGCTGTCCTTGACGCCAGTA ACCAGATTTCTGTCTACAAGTGTGATGATAAACCAGACATGGATTCCACAGTGAAACTAGGAGCTGTGGG TGGAACTGGATTTAAAGTTCCTCTTAGAACACCTCATCTGGAAAAAAGATACAGGATTCAGTTTGGGAAC AAGGAAGAGGAGGAGGATGTAAGCCCACTGCAATTTCGATTTCTCACCTGGATTGAAGGTGACGCTTTCC TGGCCATAAGCCACAGTCACTCCAGCCCACAGTCCATCATTCACCATCTGACTATGGCCGGTTCTGAGGG GGATGAGGAACAAGGACAGCTGAATGTCAGTTCATCTGTGACAGTGGATGGAGTTGTCATTGGTTTATGT TGCTGTTCTAAGACCAAGTCATCAGCAGTACAGCTGGCTGACGGCCAGGTGCTCAAGTACCTTTGGGAGT CGCCTTCTTCGGCTGTGGAGCCCTGGAAGAACTCTGAAGGGCGTCCTGTCCGATTTGCTCGTCCATGCAC CCAGATGGAAGCAGCCGCCATTGGAGGGGAGGAATGTGTCCTTGGTCTGACCGACAGGTGTCGCTTTTTC ATCAACGACACTGAGGTTGCATCAAATATCACGTCATTTGCAGTGTGTGATGACTTTCTACTGGTGACAA CCCATTCCCACACCTGCCAGTGTTTCTCTCTAAGTGGTGCCTCACTTAAAATGCTGCAGGCAGGTCTGTG TGGCAGTCAGGTGCCCAGTGGAGAGATTCTGAGGAAGGTGGAGAGGGGTTCCCGGATCGTCACAGTCGTC CCCCAGGACACAAAGCTTATACTCCAGATGCCGAGGGGGAACTTGGAGGTTGTTCATCATCGGGCCTTGG TCTTGGCTCAGATTCGGAAGTGGCTGGACAAACTTATGTTTAAAGAAGCGTTTGAATGCATGAGGAAACT GAGAATTAACCTCAATCTGATTCATGATCATAACCCCAAGGTGTTTCTTGAAAATGTGGAAACCTTCATA AAACAGATTGATTCTGTAAATCATCTAAACTTGTTTTTCACAGAACTAAAGGAAGAAGATGTCACAAAGA CCATGTACCCTCCCCCGGTTACCAAGAGTGTCCAGGTGTCCACAAATCCTGATGGAAAGAAAGTTGACCT CATCTGTGACGCTATGAGAGTGGCCATGGAGACCATCAATCCTCGCAAGTTCTGCCTGTCCATACTCACA TCTCATGTGAAGAAGACGACTCCAGAACTGGACATTGTGCTGCAGAAGGTACACGAACTTCAAGGAAAAA TTCCCTTTGTTCCTGAGAGTGTGAGTGCAGAGGAGGCTTTGAAATACCTGCTGCTCCTGGTAGATGTTAA TGAATTATTTAATCATTCTCTTGGGACCTATGACTTTGATTTGGTCCTCATGGTAGCTGAGAAGTCACAA AAGGATCCTAAGGAATATCTTCCATTTCTTAATACACTTAAGAAAATGGAAACTAATTACCAACGGTTCA CCATAGACAAGTACTTGAAACGATATGAAAAGGCCCTTGGACACCTCAGCAAGTGTGGACCTGAGTATTT CACAGAATGCTTAAATTTGATAAAAGATAAAAACTTGTATAAAGAAGCTCTGAAGTTATATCGGCCAGAC TCACCACAGTACCAGGCGGTCAGTGTTGCTTATGGCGAGCACCTGGTGCAGGAGCTCCTGTACGAGCCAG CAGGGCTTGTGTTTGCCCGCTGTGGGGCCCATGAGAAGGCTCTGGAAGCGTTTTTGGCTTGTGGCAGCTG GCAGCAAGCCCTTTGTATGGCTGCCCAGCTTCAGATGGCCAAAGACAAGGTGGCTGGCCTTGCTAGAACT CTGGCAGGAAAGCTGGTCGAGCAGAGGAAACACAGTGAGGCAGCAACAGTCCTGGAACAGTACGCCCTGG ATTATGAAGAAGCCGTGCTCTTGCTGCTAGAAGGATCTGCCTGGGAAGAAGCTCTGAGGCTGGTATACAA GTATGACAGAGTGGATATTATAGAAACCAGCGTAAAGCCTTCCATTTTAGAAGCCCAAAAAAATTACATG GACTTTCTGGACTCTCAGACAGCCACATTCATTCGCCATAAAAACCGCTTGAAGGTGGTTCGAGAGCTCA AGAGCCAACGACCACGGGTGCATGTGGATCACGAGGTGGCCCATGGCCGAGAGACGGACCTCTTCTCGGA AACCAGCAGTATTAGGAGTGGCAGCGAGATGAGTGGCAGATACTCCCACAGTAACTCCAGGATATCTGCG AGGTCATCTAAAAACCGCCGCAAGGCAGAACGGAAGAAGCATAGCCTCAAAGAAGGGAGCCCCCTGGAGG GCCTGGCACTTCTGGAGGCGCTGAGTGAGGTGGTACAGAGCATTGAGAAGCTGAAAGATGAAGTGCACGC TATTTTAAAGGTGCTCTTTCTCTTTGAGTTCGAAGAGCAAGCAAGGGAGTTACAGAGGGCCTTTGAGAGT ACTCTGCAGCTGATGGGGACGGCACTTCCAGACATCTGGACTCTCACCGGCCAACAGAGTTCATCCACAC CTGTCCTAGGTCCCAGTTCCACTGTGAATAGTATCATGGCTTCTTACCAGCAGCAGAAGACTTGTGTCCC TGTTCTCGATGCTTGGGCTTGTTTGCCACCGAAGATCGACCAGAGAAGCCAGTGGAAGCTGAGCCTGCTG GAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203572 representing NM_080899 Red=Cloning site Green=Tags(s) MRNLKLHQTLEFRDIQAPGKPQCFCLRAEQGTVLIGSERGLTEVDPVKKEVKTEISLVAEGFLPEDGSGC IVGIQDLLDQESVCVATASGDVIVCNVSTQQLECVGNVASGISVMSWSPDQELLLLATAQQTLIMMTRDY EVITEQQIHQDDFGEGKFITVGWGSKDTQFHGSEGRPITFPVQMHESALSWDDHRPQITWRGDGQFFAVS VVCSQTGARKIRVWNREFALQSTSESVPGLGPSLAWKPSGSLIASTQDKPNQQDVVFFEKNGLLHGYFTL PFLKDEVKVNDLLWNADSSVLAVWLEDLPKEGSSTLKSYVQLWTVGNYHWYLKQSLPFSTTGKNQIVSLL WDPVTPGRLHVLCQGWRYLCCDWHWTTDRSSGNSADDLANVAVIDGNKVLVTVFQRTVTPPPMCTYRLLI PHPVNQVMSSAHLGNDLAVLDASNQISVYKCDDKPDMDSTVKLGAVGGTGFKVPLRTPHLEKRYRIQFGN KEEEEDVSPLQFRFLTWIEGDAFLAISHSHSSPQSIIHHLTMAGSEGDEEQGQLNVSSSVTVDGVVIGLC CCSKTKSSAVQLADGQVLKYLWESPSSAVEPWKNSEGRPVRFARPCTQMEAAAIGGEECVLGLTDRCRFF INDTEVASNITSFAVCDDFLLVTTHSHTCQCFSLSGASLKMLQAGLCGSQVPSGEILRKVERGSRIVTVV PQDTKLILQMPRGNLEVVHHRALVLAQIRKWLDKLMFKEAFECMRKLRINLNLIHDHNPKVFLENVETFI KQIDSVNHLNLFFTELKEEDVTKTMYPPPVTKSVQVSTNPDGKKVDLICDAMRVAMETINPRKFCLSILT SHVKKTTPELDIVLQKVHELQGKIPFVPESVSAEEALKYLLLLVDVNELFNHSLGTYDFDLVLMVAEKSQ KDPKEYLPFLNTLKKMETNYQRFTIDKYLKRYEKALGHLSKCGPEYFTECLNLIKDKNLYKEALKLYRPD SPQYQAVSVAYGEHLVQELLYEPAGLVFARCGAHEKALEAFLACGSWQQALCMAAQLQMAKDKVAGLART LAGKLVEQRKHSEAATVLEQYALDYEEAVLLLLEGSAWEEALRLVYKYDRVDIIETSVKPSILEAQKNYM DFLDSQTATFIRHKNRLKVVRELKSQRPRVHVDHEVAHGRETDLFSETSSIRSGSEMSGRYSHSNSRISA RSSKNRRKAERKKHSLKEGSPLEGLALLEALSEVVQSIEKLKDEVHAILKVLFLFEFEEQARELQRAFES TLQLMGTALPDIWTLTGQQSSSTPVLGPSSTVNSIMASYQQQKTCVPVLDAWACLPPKIDQRSQWKLSLL E TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_080899
ORF Size	3993 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_080899.1, NP_543175.1
RefSeq Size	4645 bp
RefSeq ORF	3996 bp
Locus ID	140934
UniProt ID	Q8VHU4
MW	149.2 kDa
Gene Summary	mutation of gene is associated with autosomal recessive disorder familial dysautonomia (FD) [RGD, Feb 2006]

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