Pom121 (NM_053622) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR203649 representing NM_053622 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTCCGGCGGCTGCGGCGGCTGACGGAGGCGAGCGGCGGCGGCCTCCGTTGGGCGTACGGGAAGGCC GGGGCCGGACCCGCGGCTGCGGAGGACCAGCGGGTGCCGCGGCACTGGGCCTAGCGCTGCTCGGCCTCGC GCTCTACCTAGTGCCCGCGGCGGCCGCGCTGGCCTGGCTGGCCGTGGGGGCGAGCGCGGCCTGGTGGGGC CTGAGCCGAGAACCCCGCGGCCCGAGGGGCCTGTCGTCGTTCGTGCGCGAGTCGCGCCGTCACCCGCGGC CCGCGCTCACCGCTTCACCGCTTCCAGCCAAGTCGCCAGTCAACGGTAGCCTCTGCGAACCCCGCAGCCC GCTTGGAGGCCCCGACCCCGCTGAACTCTTACTCATGGGCAGCTACCTGGGCAAGCCAGGCCCGCCGGAG CCTGCCCTCCCGCAGGACCCTAGAGACAGGCCAGGGCGCCGCCCACCCTCACGGTCCCCGCCGTCGTCTT CGACGGCCCAGCGAGTCCACCACGTCTACCCCGCGCTCCCCACCCCACTTTTGCGACCTTCTCGGAGGCC GCCCCACCGAGATTGTGGGCCTTTATCCAGTCGGTTTGTTATAACACCCCGAAGACGATATCCCATTCAG CAAGCCCAGTATTCCTTGCTGGGGGCACTTCCAACCGTATGCTGGAATGGTGGCCATAAGAAAGCCGTGC TATCTGCACGGAACTCGAGGATGGTGTGCAGCCCAGTGACAGTGAGGATTGCCCCTCCAGACAGCAAGCT ATTTCGATCACCAATGCCGGAGCAGATACTCAGCACCACACTGTCTTCACCATCAAGTAATGCCCCAGAC CCTTGTGCAAAGGAGACGGTGCTGAATGCCCTCAAGGAAAAGAAGAAAAGGACAGTGGCAGAGGAAGACC AGCTGCATCTTGATGGCCAGGAAAACAAGAGAAGACGCCACGATAGCAGTGGGAGTGGACATTCGGCATT CGAGCCCCTTGTGGCCAACGGAGTCCCTGCTGCATTTGTGCCTAAGCCTGGCTCCCTGAAGAGAAGTCTG GCTTCCCAGAGCTCGGATGACCACTTGAACAAACGGTCTCGCACCTCCTCTGTGAGCTCCCTGACTAGCA CATGCACAGGAGGCATCCCCAGCTCCAGCCGCAACGCCATCACCAGCTCCTACAGCTCCACCCGAGGTGT CTCACAGCTGTGGAAGAGGAGTGGTCCCACCTCTTCTCCCTTCTCCAGCCCAGCTTCCTCCCGCTCCCAG ACACCAGAGAGGCCAGCCAAGAAGACAAGAGAGGAGGAACCATGTCACCAGTCCAGTTCTTCAGCTCCCT TGGTGACAGACAAGGAGTCCCCAGGAGAAAAGGTTACAGATCCAGCCACGGGGAAGCAGCAGAGCCTGTG GACTTCCCCACCCACACCTGGCAGCTCCGGGCAACGCAAACGCAAGATTCAGCTGCTGCCCTCCCGGCGA GGGGACCAGCTCACCTTGCCTCCACCCCCTGAGCTTGGCTATTCCATCACTGCTGAAGACCTGGACATGG AGAGGAGAGCCTCACTGCAGTGGTTTAACAAGGTCTTGGAGGATAAGACGGATGATGCCTCTACCCCAGC CACTGACACCTCCCCAGCCACCAGCCCTCCCTTCACACTCACCCTGCCTACCGTGGGGCCTGCTGCCTCA CCAGCCTCCCTCCCAGCTCCCAGCTCTAACCCACTGTTGGAGAGTTTGAAGAAGATGCAGGAGTCTCCAG CCCCATCCTCCTCAGAACCTCCTGAGGCAGCAACAGTTGCGGCCCCTTCACCTCCGAAGACACCTAGCCT CCTGGCCCCTCTTGTCTCACCACTGACAGGGCCCCTAGCCAGCACCTCCTCAGACTCCAAACCTACAACC ACCTTCCTGGGGCTGGCTTCTGCTTCATCCGCCACACCACTCACTGACACCAAGGCCCCTGGAGTCTCTC AGGCCCAGCTGTGTGTCAGCACCCCAGCCGCCACAGCTCCCTCCCCAACTCCAGCCAGCACGCTGTTTGG GATGCTGAGTCCACCTGCCAGCTCCTCTTCCCTTGCCACTCCTGGCCCAGCTTGCGCTTCTCCCATGTTT AAGCCCATTTTCCCGGCCACACCTAAGAGTGAGAGTGATAATCCCTTGCCAACTAGCTCCTCAGCCGCCA CGACAACTCCAGCCAGCACCGCCCTCCCCACAACAGCAACCGCCACAGCTCACACTTTCAAGCCCATTTT TGAGAGCGTGGAGCCATTTGCAGCCATGCCCCTGTCACCTCCCTTCTCCCTGAAGCAGACAACTGCTCCA GCCACCACTGCAGCCACATCAGCTCCTCTCCTCACTGGCCTGGGCACTGCCACTTCCACAGTGGCCACTG GCACCACAGCTTCTGCCTCCAAGCCTGTGTTTGGCTTTGGAGTGACCACTGCAGCGAGCACTGCCAGTAC CATAGCTTCCACCTCCCAGTCAATTCTGTTTGGGGGAGCCCCGCCTGTTACTGCTTCCAGTTCCGCCCCA GCCCTGGCCTCCATCTTCCAATTTGGCAAGCCCCTTGCCCCAGCAGCGTCTGTGGCGGGCACCTCCTTTA GCCAGTCCCTTGCCAGCTCTGCCCAGACTGCTGCCAGCAATAGTAGTGGGGGCTTCAGTGGTTTTGGTGG GACCCTCACCACCTCCACCTCTGCCCCTGCCACCACCAGCCAGCCCACTCTGACCTTCAGCAACACTGTC ACCCCCACCTTCAACATTCCCTTTAGCGCCAGTGCCAAGCCTGCGCTCCCAACCTACCCCGGAGCCAACT CACAGCCCACATTTGGAGCCACTGATGGGGCCACCAAGCCGGCACTTGCCCCAAGTTTTGGCAGCTCTTT CACTTTTGGCAACTCTGTGGCCTCAGCCCCGTCGGCAGCCCCAGCACCAGCCGCCTTTGGTGGTGCTGCA CAGCCAGCTTTCGGAGGGTTGAAAGCCTCAGCCTCCACCTTCGGCACCCCTGCCAGCACTCAGCCGGCTT TCGGTAGCACCACCTCCGTGTTCTCCTTTGGTTCAGCCACCACATCTGGCTTTGGTGCTGCTGCCGCCAC CACACAGACCACCCACAGTGGGAGCAGCAGCTCTCTGTTTGGCAGCTCTACTCCATCCCCATTCACATTC GGTGGCTCAGCAGCTCCTGCTGGTGGTGGAGGCTTTGGGCTTAGTGCTACACCAGGTACCGGCTCCACCT CTGGAACTTTTAGCTTTGGATCTGGACAGAGCGGGACCACGGGCACCACCACCTCCTTTGGGGGAAGTCT GAGTCAGAACACCCTGGGCGCACCTAGCCAGAGCTCACCGTTCGCCTTCAGTGTGGGCAGTACACCTGAA AGCAAGCCTGTGTTTGGAGGCACATCCACACCTACTTTTGGGCAGAGTGCCCCTGCCCCAGGAGTCGGCA CCACAGGCAGCAGCCTCTCATTTGGGGCCCCTTCAACACCTGCCCAAGGCTTTGTTGGAGTTGGACCTTT CGGATCAGGAGCCCCTTCCTTTTCCATTGGCGCGGGATCCAAGACCCCAGGGGCTCGACAGCGACTTCAG GCCCGAAGGCAGCACACCCGCAAGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR203649 representing NM_053622 Red=Cloning site Green=Tags(s) MSPAAAAADGGERRRPPLGVREGRGRTRGCGGPAGAAALGLALLGLALYLVPAAAALAWLAVGASAAWWG LSREPRGPRGLSSFVRESRRHPRPALTASPLPAKSPVNGSLCEPRSPLGGPDPAELLLMGSYLGKPGPPE PALPQDPRDRPGRRPPSRSPPSSSTAQRVHHVYPALPTPLLRPSRRPPHRDCGPLSSRFVITPRRRYPIQ QAQYSLLGALPTVCWNGGHKKAVLSARNSRMVCSPVTVRIAPPDSKLFRSPMPEQILSTTLSSPSSNAPD PCAKETVLNALKEKKKRTVAEEDQLHLDGQENKRRRHDSSGSGHSAFEPLVANGVPAAFVPKPGSLKRSL ASQSSDDHLNKRSRTSSVSSLTSTCTGGIPSSSRNAITSSYSSTRGVSQLWKRSGPTSSPFSSPASSRSQ TPERPAKKTREEEPCHQSSSSAPLVTDKESPGEKVTDPATGKQQSLWTSPPTPGSSGQRKRKIQLLPSRR GDQLTLPPPPELGYSITAEDLDMERRASLQWFNKVLEDKTDDASTPATDTSPATSPPFTLTLPTVGPAAS PASLPAPSSNPLLESLKKMQESPAPSSSEPPEAATVAAPSPPKTPSLLAPLVSPLTGPLASTSSDSKPTT TFLGLASASSATPLTDTKAPGVSQAQLCVSTPAATAPSPTPASTLFGMLSPPASSSSLATPGPACASPMF KPIFPATPKSESDNPLPTSSSAATTTPASTALPTTATATAHTFKPIFESVEPFAAMPLSPPFSLKQTTAP ATTAATSAPLLTGLGTATSTVATGTTASASKPVFGFGVTTAASTASTIASTSQSILFGGAPPVTASSSAP ALASIFQFGKPLAPAASVAGTSFSQSLASSAQTAASNSSGGFSGFGGTLTTSTSAPATTSQPTLTFSNTV TPTFNIPFSASAKPALPTYPGANSQPTFGATDGATKPALAPSFGSSFTFGNSVASAPSAAPAPAAFGGAA QPAFGGLKASASTFGTPASTQPAFGSTTSVFSFGSATTSGFGAAAATTQTTHSGSSSSLFGSSTPSPFTF GGSAAPAGGGGFGLSATPGTGSTSGTFSFGSGQSGTTGTTTSFGGSLSQNTLGAPSQSSPFAFSVGSTPE SKPVFGGTSTPTFGQSAPAPGVGTTGSSLSFGAPSTPAQGFVGVGPFGSGAPSFSIGAGSKTPGARQRLQ ARRQHTRKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_053622
ORF Size	3597 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_053622.1, NP_446074.1
RefSeq Size	5508 bp
RefSeq ORF	3600 bp
Locus ID	113975
UniProt ID	P52591
MW	120.8 kDa
Gene Summary	may act to anchor components of the nuclear complex to the pore membrane domain of the nuclear envelope [RGD, Feb 2006]

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