United States
Germany
Japan
United Kingdom
China
Vcl (NM_001107248) Rat Tagged ORF Clone
CAT＃: RR203691
- TrueORF®
Vcl (Myc-DDK-tagged ORF) - Rat vinculin (Vcl), (10 ug)
"NM_001107248" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 17,100.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203691 representing NM_001107248
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGTTTCACACGCGCACGATCGAGAGCATCCTGGAGCCGGTGGCGCAGCAGATCTCTCACCTGG TGATTATGCACGAGGAGGGCGAGGTGGACGGCAAAGCCATTCCTGACCTCACCGCGCCCGTGGCCGCTGT GCAGGCGGCCGTCAGCAACCTCGTCCGGGTTGGGAAAGAGACCGTTCAGACCACTGAGGATCAGATTTTG AAGAGAGATATGCCGCCAGCATTTATTAAGGTCGAGAATGCTTGCACGAAGCTTGTTCAGGCAGCTCAGA TGCTTCAGTCAGACCCATACTCTGTTCCTGCGCGGGATTACCTCATCGACGGCTCCAGGGGAATCCTCTC TGGAACGTCTGACCTCCTGCTTACCTTTGATGAGGCTGAGGTTCGAAAAATTATAAGAGTTTGCAAAGGG ATTTTGGAATACCTCACAGTGGCAGAGGTAGTGGAGACTATGGAAGACTTGGTCACTTACACAAAGAACC TTGGGCCCGGAATGACGAAGATGGCCAAAATGATCGATGAGAGACAGCAGGAGTTGACGCACCAGGAACA CCGTGTGATGCTGGTGAACTCAATGAACACTGTCAAAGAGCTGCTTCCTGTTCTCATTTCAGCTATGAAG ATTTTTGTTACAACCAAAAACTCAAAAAACCAAGGAATAGAAGAAGCTCTGAAAAATCGAAATTTTACTG TAGGAAAGATGAGTGCTGAGATTAACGAGATCATTCGTGTGTTACAACTCACCTCCTGGGATGAGGACGC CTGGGCCAGCAAGGACACTGAAGCCATGAAGAGAGCGCTGGCCTCCATAGACTCGAAACTGAACCAGGCC AAAGGTTGGCTCCGTGACCCCAACGCTTCCCCAGGGGACGCCGGTGAGCAGGCCATCAGGCAGATCTTGG ACGAAGCTGGAAAAGTTGGTGAACTTTGTGCAGGCAAGGAACGCAGGGAGATCCTAGGAACCTGCAAGAT GCTAGGGCAGATGACTGACCAAGTGGCTGACCTCCGAGCCAGAGGACAAGGAGCCTCGCCAGTGGCCATG CAGAAGGCCCAGCAAGTGTCTCAGGGGCTCGACGTGCTTACTGCGAAAGTGGAAAATGCAGCTCGAAAGC TGGAAGCCATGACGAACTCAAAGCAGAGTATTGCGAAGAAGATTGACGCTGCGCAGAATTGGCTGGCAGA TCCCAATGGTGGACCTGAAGGAGAAGAACAGATCCGAGGGGCTTTGGCTGAAGCTCGGAAGATTGCTGAG TTATGTGATGATCCTAAGGAGAGGGACGACATCCTCCGCTCTCTTGGAGAGATAGCGGCTCTGACCTCTA AATTAGGAGACTTGCGAAGACAGGGGAAAGGAGACTCCCCAGAGGCTCGAGCCTTGGCTAAACAAGTGGC AACCGCGCTACAGAACCTGCAGACCAAAACCAATAGGGCTGTGGCCAACAGCAGGCCTGCCAAGGCAGCC GTCCACCTGGAAGGCAAGATTGAGCAGGCACAGCGGTGGATTGATAACCCTACAGTGGATGACCGAGGAG TCGGTCAGGCTGCCATCCGTGGACTTGTGGCTGAAGGGCATCGGCTGGCCAATGTCATGATGGGACCTTA TCGCCAAGATCTTCTCGCCAAGTGTGACCGTGTAGACCAGCTAGCAGCTCAGCTGGCTGACCTGGCTGCC CGAGGGGAGGGGGAGAGTCCTCAGGCGAGAGCGCTTGCTTCTCAGCTTCAGGACTCCTTAAAGGATCTTA AAACCCAGATGCAGGAAGCTATGACTCAAGAGGTGTCCGACGTTTTCAGCGATACTACAACTCCCATCAA GCTGTTGGCAGTGGCGGCCACTGCTCCTCCTGATGCGCCCAATAGGGAAGAGGTATTTGACGAGAGAGCA GCCAACTTTGAAAACCATTCAGGAAGGCTTGGGGCCACGGCAGAGAAGGCAGCTGCTGTTGGTGCTGCCA ATAAATCGACAGTGGAAGGCATTCAGGCATCCGTGAAGACAGCCCGAGAACTCACTCCCCAGGTGATCTC AGCTGCTCGAATCTTACTGAGGAACCCTGGTAACCAAGCTGCGTATGAACATTTTGAGACCATGAAGAAC CAGTGGATTGATAATGTTGAGAAAATGACAGGGCTGGTGGACGAGGCCATTGATACCAAATCTCTGTTGG ATGCTTCTGAAGAAGCAATTAAAAAAGACCTGGACAAGTGTAAGGTAGCCATGGCCAATATTCAGCCTCA GATGCTGGTCGCTGGAGCAACCAGTATTGCTCGTCGGGCCAACCGGATTCTGCTGGTTGCTAAGAGGGAG GTAGAGAACTCTGAGGATCCGAAGTTCCGAGAGGCTGTGAAAGCTGCCTCTGATGAACTGAGCAAAACAA TCTCCCCCATGGTTATGGATGCCAAGGCTGTGGCTGGAAACATCTCTGACCCTGGCCTGCAAAAGAGCTT CCTGGACTCAGGATATCGGATCCTGGGAGCTGTGGCCAAAGTTAGAGAAGCCTTCCAACCTCAGGAGCCT GACTTCCCGCCTCCTCCACCAGACCTTGAACAGCTACGTCTAACTGATGAGCTGGCTCCTCCCAAACCAC CTCTGCCTGAGGGTGAAGTCCCTCCACCCAGGCCACCCCCACCAGAAGAGAAGGATGAAGAGTTCCCTGA GCAGAAAGCCGGGGAGGTGATTAACCAGCCGATGATGATGGCCGCCAGGCAGCTCCACGATGAAGCTCGG AAGTGGTCTAGCAAGGGCAATGACATCATCGCAGCAGCCAAGCGCATGGCTCTGCTGATGGCGGAGATGT CTCGGCTGGTAAGAGGGGGCAGTGGTACCAAGCGGGCACTTATTCAGTGTGCCAAGGATATCGCCAAGGC CTCTGATGAGGTGACGAGGTTGGCCAAGGAGGTTGCCAAGCAGTGCACAGATAAGCGGATTAGAACCAAT CTCTTACAGGTCTGTGAGCGAATCCCAACTATAAGCACCCAGCTCAAAATCCTGTCCACAGTGAAGGCCA CCATGCTGGGCCGGACCAACATCAGTGACGAGGAGTCTGAGCAGGCCACAGAGATGCTGGTTCATAATGC CCAGAACCTCATGCAGTCTGTGAAAGAGACTGTGCGAGAGGCCGAAGCTGCTTCAATCAAGATTCGAACA GACGCCGGATTTACTCTGCGCTGGGTCAGAAAGACTCCCTGGTACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203691 representing NM_001107248
Red=Cloning site Green=Tags(s) MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQIL KRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKG ILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMK IFVTTKNSKNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQA KGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAM QKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAE LCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAA VHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAA RGEGESPQARALASQLQDSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERA ANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKN QWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKRE VENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEP DFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEAR KWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTN LLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRT DAGFTLRWVRKTPWYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001107248 |
| ORF Size | 3198 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001107248.1, NP_001100718.1 |
| RefSeq Size | 5270 bp |
| RefSeq ORF | 3201 bp |
| Locus ID | 305679 |
| UniProt ID | P85972 |
| MW | 116.6 kDa |
| Gene Summary | Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203691 | Vcl (untagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 17,100.00 |
|
| RR203691L3 | Lenti ORF clone of Vcl (Myc-DDK-tagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 18,720.00 |
|
| RR203691L4 | Lenti ORF clone of Vcl (mGFP-tagged ORF) - Rat vinculin (Vcl), (10 ug) |
CNY 20,520.00 |
