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Sema6b (NM_053471) Rat Tagged ORF Clone
CAT＃: RR203840
- TrueORF®
Sema6b (Myc-DDK-tagged ORF) - Rat sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), (10 ug)
"NM_053471" in other vectors (3)
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CNY 11,500.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR203840 representing NM_053471
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGACCCCGCGAGCGCCCCCTCCACGCCCGGCCCTGCTGTTCCTCCTGCTGTTGCTTCTGAGGGTCA CCCATGGCCTTTTCCCAGATGAACCACCTCCACTCAGTGTGGCTCCCAGGGACTACCTGAGCCACTACCC CGTGTTCGTGGGCAGCGGGCCTGGTCGTCTGACCCCTGCAGAGGGTGCTGAGGACCTCAACATCCAGAGA GTGCTACGTGTTAACAGGACACTGTTCATCGGGGACAGAGACAACCTGTACCAAGTAGAACTGGAGCCAT CCACATCCACGGAGCTGCGGTATCAGCGGAAGCTTACCTGGCGCTCCAACCCCAGTGACATCGATGTGTG TCGGATGAAGGGCAAGCAAGAGGGTGAGTGTCGGAACTTTGTCAAGGTGCTCCTGCTTCGTGACGAATCC ACGCTCTTCGTGTGCGGCTCCAATGCATTCAATCCCATCTGTGCCAATTACAGTATGGACACACTGCAGC TTCTTGGAGACAACATCAGTGGTATGGCCCGCTGCCCCTACGACCCCAAGCATGCCAATGTCGCCCTCTT CTCAGATGGGATGCTCTTCACAGCCACAGTAACTGACTTCCTAGCCATCGACGCTGTTATCTACCGTAGC CTTGGGGACCGGCCCACACTGCGCACAGTAAAGCATGACTCCAAGTGGTTTAAAGAGCCATACTTTGTGC ATGCGGTGGAGTGGGGAAGCCACGTCTACTTCTTCTTCCGGGAGATCGCCATGGAGTTTAACTATCTGGA AAAGGTGGTGGTGTCCCGTGTGGCCCGTGTATGCAAGAATGATGTGGGCGGCTCCCCACGGGTGCTGGAG AAGCAGTGGACTTCCTTCCTGAAGGCCCGGCTCAACTGCTCCGTGCCTGGGGACTCACACTTCTACTTCA ATGTACTGCAGGCTGTGACTGGTGTGGTGAGCCTTGGCGGCCGTCCAGTGATTCTTGCTGTCTTCTCAAC TCCTAGCAACAGCATCCCTGGCTCAGCTGTCTGTGCCTTTGACATGAACCAAGTGGCTGCTGTGTTTGAA GGCCGCTTCCGGGAGCAGAAGTCACCTGAGTCAATCTGGACCCCAGTGCCTGAGGACCAAGTACCACGGC CCAGGCCCGGGTGCTGTGCAGCGCCCGGTATGCAGTACAACGCATCCAATGCCCTTCCTGACGAGATTCT CAACTTTGTAAAGACCCACCCACTGATGGACGAAGCGGTGCCCTCCCTGGGCCACTCGCCTTGGATTGTG AGAACTCTGATACGGCACCAGCTGACCCGAGTGGCTGTGGATGTGGGTGCAGGCCCATGGGGCAATCAGA CAATAGTCTTCCTTGGCTCTGAGGTTGGCACAGTCCTCAAATTCCTTGTGAAGCCCAATGCCAGTGTCTC AGGGACCACAGGGCCCAGCATCTTTTTGGAGGAGTTTGAGACCTACCGGCCAGACAGGTGTGGACGATCC AGCAGTGCTGGTGAGTGGGGACAACGACTTCTGAGCCTGGAGCTAGATGCTGCCTCAGGTGGCCTGCTGG CAGCCTTCCCCCGCTGTGTGGTTCGTGTTCCTGTTGCCCGCTGCCAGCTGTACTCGGGGTGCATGAAGAA CTGCATTGGCAGCCAAGATCCATACTGCGGGTGGGCCCCCGATGGCTCCTGCATCTTCCTCAGACCAGGA ACCAGTGCCACGTTTGAGCAAGATGTGTCCGGGGCCAGCACCTCTGGCTTAGGTGACTGCACTGGACTCT TGCGCGCCAGCCTCTCAGATGACCGCGCAGGGCTGGTATCGGTGAACCTGCTGGTGACGTCCTCGGTGGC AGCGTTCGTGGTGGGTGCCGTGGTGTCCGGTTTCAGCGTGGGCTGGTTCGTGGGTCTCCGCGAGCGGCGG GAGCTGGCCCGGCGCAAGGACAAGGAAGCCATCCTGGCGCATGGCGGCAGCGAGGCAGTGCTGAGCGTGA GCCGACTGGGCGAGCGCAGGGGAACCGGGACTGGGGGTCGTGGGGGAGCCGGCGGCGGTCCCGGGGGTCC CCCGGAGGCCCTGCTGGCCCCGCTTATGCAGAATGGCTGGACAAAGGCGGCACTGCTGCATGGTGGTCCT CACGACCTGGATTCGGGGCTGCTGCCCACCCCTGAGCAGACGCCTCTGCCCCAGAAACGCCTGCCCACAA CACACCCACACGCCCACGCCCTAGGACCGCGAGCCTGGGACCACAGCCACGCGCTGCTGTCGGCCTCTGC CTCCACATCCTTGCTCCTGCTGGCGCACACCCGCGCCCCTGAGCAGCCCCCAGTGCCGACTGAGTCAGGC CCGGAATCTCGCCTCTGCGCCCCAAGATCCTGCCGGGCCTCTCACCCAGGCGACTTCCCACTCACGCCTC ACGCCAGCCCGGACCGCCGGCGGGTTGTGTCCGCACCCACGGGCCCCTTGGACTCTTCTTCGGTGGGCGA CGACCTTCCAGGGCCATGGAGCCCACCTGCAACCAGCAGCCTGCGGAGGCCGGGCCCCCATGGGCCCCCA ACAGCCGCCCTGCGGCGCACACACACATTCAACAGCGGCGAGGCGAGGCCCGGGGGTCACCGTCCTCGCC GCCACGCACCCGCGGACTCTACACACTTGCTGCCCTGCGGGACGGGCGAGCGGACTGCACCCCCGGTACC C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR203840 representing NM_053471
Red=Cloning site Green=Tags(s) MWTPRAPPPRPALLFLLLLLLRVTHGLFPDEPPPLSVAPRDYLSHYPVFVGSGPGRLTPAEGAEDLNIQR VLRVNRTLFIGDRDNLYQVELEPSTSTELRYQRKLTWRSNPSDIDVCRMKGKQEGECRNFVKVLLLRDES TLFVCGSNAFNPICANYSMDTLQLLGDNISGMARCPYDPKHANVALFSDGMLFTATVTDFLAIDAVIYRS LGDRPTLRTVKHDSKWFKEPYFVHAVEWGSHVYFFFREIAMEFNYLEKVVVSRVARVCKNDVGGSPRVLE KQWTSFLKARLNCSVPGDSHFYFNVLQAVTGVVSLGGRPVILAVFSTPSNSIPGSAVCAFDMNQVAAVFE GRFREQKSPESIWTPVPEDQVPRPRPGCCAAPGMQYNASNALPDEILNFVKTHPLMDEAVPSLGHSPWIV RTLIRHQLTRVAVDVGAGPWGNQTIVFLGSEVGTVLKFLVKPNASVSGTTGPSIFLEEFETYRPDRCGRS SSAGEWGQRLLSLELDAASGGLLAAFPRCVVRVPVARCQLYSGCMKNCIGSQDPYCGWAPDGSCIFLRPG TSATFEQDVSGASTSGLGDCTGLLRASLSDDRAGLVSVNLLVTSSVAAFVVGAVVSGFSVGWFVGLRERR ELARRKDKEAILAHGGSEAVLSVSRLGERRGTGTGGRGGAGGGPGGPPEALLAPLMQNGWTKAALLHGGP HDLDSGLLPTPEQTPLPQKRLPTTHPHAHALGPRAWDHSHALLSASASTSLLLLAHTRAPEQPPVPTESG PESRLCAPRSCRASHPGDFPLTPHASPDRRRVVSAPTGPLDSSSVGDDLPGPWSPPATSSLRRPGPHGPP TAALRRTHTFNSGEARPGGHRPRRHAPADSTHLLPCGTGERTAPPVP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_053471 |
| ORF Size | 2661 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_053471.2, NP_445923.1 |
| RefSeq Size | 3847 bp |
| RefSeq ORF | 2664 bp |
| Locus ID | 84609 |
| UniProt ID | O70141 |
| MW | 95.8 kDa |
| Gene Summary | may play roles in neurogenesis and axon guidance [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN203840 | Sema6b (untagged ORF) - Rat sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), (10 ug) |
CNY 11,500.00 |
|
| RR203840L3 | Lenti ORF clone of Sema6b (Myc-DDK-tagged ORF) - Rat sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), (10 ug) |
CNY 13,110.00 |
|
| RR203840L4 | Lenti ORF clone of Sema6b (mGFP-tagged ORF) - Rat sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B (Sema6b), (10 ug) |
CNY 14,350.00 |
