Capza1 (NM_001109625) Rat Tagged ORF Clone
CAT#: RR204065
- TrueORF®
Capza1 (Myc-DDK-tagged ORF) - Rat capping protein (actin filament) muscle Z-line, alpha 1 (Capza1), (10 ug)
"NM_001109625" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204065 representing NM_001109625
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGACTTCGAGGATCGGGTGTCGGATGAGGAGAAGGTACGCATAGCCGCTAAATTCATCACCCATG CACCCCCAGGGGAGTTCAATGAAGTATTCAATGATGTCCGGCTACTGCTTAATAATGACAATCTTCTCAG GGAAGGGGCAGCACATGCATTTGCCCAGTATAACATGGATCAGTTCACACCTGTGAAGATAGAAGGCTAC GACGACCAGGTCTTAATTACAGAGCATGGCGACCTTGGTAACAGCAGATTTTTAGATCCAAGAAACAAAA TTTCCTTTAAGTTTGATCATTTACGGAAAGAAGCGAGTGACCCGCAGCCAGAGGATGTGGATGGAGGCTT GAAGTCTTGGAGAGACTCGTGTGACAGTGCACTGAGAGCCTATGTGAAAGATCATTATTCCAACGGCTTC TGTACTGTTTACGCTAAAACCATAGATGGACAGCAGACCATTATTGCATGTATTGAAAGCCACCAGTTTC AACCCAAAAACTTCTGGAATGGTCGTTGGAGATCAGAGTGGAAGTTCACCATCACACCACCTACAGCCCA GGTGGTTGGAGTGCTCAAGATTCAGGTTCATTATTATGAAGATGGCAATGTTCAGCTGGTTAGTCATAAA GATGTGCAGGACTCAGTAACTGTTTCTAATGAAGTCCAAACTGCCAAGGAGTTTATTAAAATCATAGAGA GTGCAGAAAATGAGTATCAGACAGCAATTAGTGAAAATTATCAAACTATGTCAGACACCACGTTCAAGGC CTTGCGCCGGCAGCTTCCAGTTACCCGCACCAAAATCGACTGGAACAAGATACTCAGTTACAAGATTGGC AAAGAAATGCAGAATGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204065 representing NM_001109625
Red=Cloning site Green=Tags(s) MADFEDRVSDEEKVRIAAKFITHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNMDQFTPVKIEGY DDQVLITEHGDLGNSRFLDPRNKISFKFDHLRKEASDPQPEDVDGGLKSWRDSCDSALRAYVKDHYSNGF CTVYAKTIDGQQTIIACIESHQFQPKNFWNGRWRSEWKFTITPPTAQVVGVLKIQVHYYEDGNVQLVSHK DVQDSVTVSNEVQTAKEFIKIIESAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIG KEMQNA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001109625 |
ORF Size | 858 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001109625.2, NP_001103095.2 |
RefSeq Size | 3256 bp |
RefSeq ORF | 861 bp |
Locus ID | 691149 |
UniProt ID | B2GUZ5 |
MW | 32.9 kDa |
Gene Summary | F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the formation of epithelial cell junctions.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204065 | Capza1 (untagged ORF) - Rat capping protein (actin filament) muscle Z-line, alpha 1 (Capza1), (10 ug) |
CNY 3,990.00 |
|
RR204065L3 | Lenti ORF clone of Capza1 (Myc-DDK-tagged ORF) - Rat capping protein (actin filament) muscle Z-line, alpha 1 (Capza1), (10 ug) |
CNY 6,080.00 |
|
RR204065L4 | Lenti ORF clone of Capza1 (mGFP-tagged ORF) - Rat capping protein (actin filament) muscle Z-line, alpha 1 (Capza1), (10 ug) |
CNY 6,650.00 |