Hprt1 (NM_012583) Rat Tagged ORF Clone
CAT#: RR204071
- TrueORF®
Hprt1 (Myc-DDK-tagged ORF) - Rat hypoxanthine phosphoribosyltransferase 1 (Hprt1), (10 ug)
"NM_012583" in other vectors (3)
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CNY 3,990.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Hgprtase; Hprt |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204071 representing NM_012583
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGACCCTCAGTCCCAGCGTCGTGATTAGTGATGATGAACCAGGTTATGACCTAGATTTATTTTGCA TACCTAATCATTATGCTGAAGATTTGGAAAAGGTGTTTATTCCTCATGGACTGATTATGGACAGGACTGA AAGACTTGCTCGAGATGTCATGAAGGAGATGGGAGGCCATCACATTGTGGCCCTCTGTGTGCTGAAGGGG GGCTATAAGTTCTTTGCTGACCTGCTGGATTACATTAAAGCGCTGAATAGAAATAGTGATAGGTCCATTC CTATGACTGTAGATTTTATCAGACTGAAGAGCTACTGTAATGACCAGTCAACGGGGGACATAAAAGTTAT TGGTGGAGATGATCTCTCAACTTTAACTGGAAAGAACGTCTTGATTGTTGAAGATATAATTGACACTGGT AAAACAATGCAGACTTTGCTTTCCTTGGTCAAGCAGTACAGCCCCAAAATGGTTAAGGTTGCAAGCTTGC TGGTGAAAAGGACCTCTCGAAGTGTTGGATACAGGCCAGACTTTGTTGGATTTGAAATTCCAGACAAGTT TGTTGTTGGATATGCCCTTGACTATAATGAGCACTTCAGGGATTTGAATCATGTTTGTGTCATCAGCGAA AGTGGAAAAGCCAAGTACAAAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204071 representing NM_012583
Red=Cloning site Green=Tags(s) MSTLSPSVVISDDEPGYDLDLFCIPNHYAEDLEKVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKG GYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTG KTMQTLLSLVKQYSPKMVKVASLLVKRTSRSVGYRPDFVGFEIPDKFVVGYALDYNEHFRDLNHVCVISE SGKAKYKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_012583 |
ORF Size | 654 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_012583.2, NP_036715.1 |
RefSeq Size | 1260 bp |
RefSeq ORF | 657 bp |
Locus ID | 24465 |
UniProt ID | P27605 |
MW | 24.5 kDa |
Gene Summary | catalyzes the conversion of IMP and diphosphate to hypoxanthine and 5-phospho-alpha-D-ribose 1-diphosphate [RGD, Feb 2006] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Ultrasound Used for Diagnostic Imaging Facilitates Dendritic Branching of Developing Neurons in the Mouse Cortex
,null,
Frontiers in Neuroscience
,PubMed ID 35368285
[Hprt1]
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Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204071 | Hprt1 (untagged ORF) - Rat hypoxanthine phosphoribosyltransferase 1 (Hprt1), (10 ug) |
CNY 3,990.00 |
|
RR204071L3 | Lenti ORF clone of Hprt1 (Myc-DDK-tagged ORF) - Rat hypoxanthine phosphoribosyltransferase 1 (Hprt1), (10 ug) |
CNY 6,080.00 |
|
RR204071L4 | Lenti ORF clone of Hprt1 (mGFP-tagged ORF) - Rat hypoxanthine phosphoribosyltransferase 1 (Hprt1), (10 ug) |
CNY 6,650.00 |