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Sh3glb1 (NM_001011929) Rat Tagged ORF Clone
CAT＃: RR204100
- TrueORF®
Sh3glb1 (Myc-DDK-tagged ORF) - Rat SH3-domain GRB2-like endophilin B1 (Sh3glb1), (10 ug)
"NM_001011929" in other vectors (3)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204100 representing NM_001011929
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATCATGGATTTCAACGTGAAGAAGCTGGCGGCCGACGCGGGCACTTTCCTCAGCCGGGCCGTGC AGTTCACAGAGGAAAAGCTTGGCCAAGCAGAGAAGACAGAACTGGATGCTCACCTGGAAAATCTCCTCAG CAAGGCGGAGTGCACCAAAGTGTGGACAGAGAAGATAATGAAGCAGACTGAGGTGCTGTTGCAGCCAAAT CCAAATGCCAGGATAGAAGAATTTGTTTATGAGAAACTGGACAGAAAAGCGCCAAGTCGTATAAACAACC CAGAACTTTTGGGACAGTATATGATTGATGCAGGCACTGAGTTTGGCCCAGGGACAGCTTATGGTAATGC CCTTATTAAATGTGGAGAAACACAGAAGCGAATTGGAACAGCTGACAGAGAGCTGATTCAAACATCAGCC TTAAATTTCCTCACTCCTTTAAGAAACTTTATAGAAGGGGATTACAAGACAATCGCAAAAGAAAGGAAGC TATTACAGAATAAGAGACTGGATTTGGATGCTGCAAAAACAAGACTAAAAAAGGCAAAAGCTGCAGAAAC TAAAAGTTCATCTGAACAGGAATTAAGAATAACTCAAAGTGAATTTGATCGTCAGGCAGAGATTACCCGT CTTCTGCTGGAAGGAATCAGCAGTACACACGCCCATCATCTCCGCTGTCTGAATGACTTTGTAGAAGCCC AGATGACTTACTATGCACAATGTTACCAGTACATGTTAGACCTACAAAAACAACTGGGAAGTTTTCCATC CAATTATGTTTCTAACAACAATCAGACTTCTGGGACACCTGTGCCGTATACTTTGTCAAATACCATTGGT CCTTCTGCTGTGGCTTCAACAGGTAGCCTGGTAATCACCTGTCCTCCTAACCTCAGTGACCTTAAAGACT CCAGCAGCACCAGGAAGGCCAGGGTGCTGTACGATTATGACGCTGCCAACAGTACGGAGCTGTCACTCCT GGCAGACGAGGTAATCACTGTGTTCAGTGTCGTTGGAATGGACTCCGACTGGCTAATGGGGGAGAGAGGA AATCAGAAGGGCAAGGTGCCAATTACCTACTTAGAGCTTCTCAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204100 representing NM_001011929
Red=Cloning site Green=Tags(s) MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKVWTEKIMKQTEVLLQPN PNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSA LNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETKSSSEQELRITQSEFDRQAEITR LLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYVSNNNQTSGTPVPYTLSNTIG PSAVASTGSLVITCPPNLSDLKDSSSTRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERG NQKGKVPITYLELLN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001011929 |
| ORF Size | 1095 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001011929.1, NP_001011929.1 |
| RefSeq Size | 1795 bp |
| RefSeq ORF | 1098 bp |
| Locus ID | 292156 |
| UniProt ID | Q6AYE2 |
| MW | 40.8 kDa |
| Gene Summary | May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation. Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204100 | Sh3glb1 (untagged ORF) - Rat SH3-domain GRB2-like endophilin B1 (Sh3glb1), (10 ug) |
CNY 3,656.00 |
|
| RR204100L3 | Lenti ORF clone of Sh3glb1 (Myc-DDK-tagged ORF) - Rat SH3-domain GRB2-like endophilin B1 (Sh3glb1), (10 ug) |
CNY 6,080.00 |
|
| RR204100L4 | Lenti ORF clone of Sh3glb1 (mGFP-tagged ORF) - Rat SH3-domain GRB2-like endophilin B1 (Sh3glb1), (10 ug) |
CNY 6,650.00 |
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