Myh7b (NM_001107794) Rat Tagged ORF Clone

Specifications

Product Data
Type	Rat Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RR204114 representing NM_001107794 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGATATGAATGAACTTGGAGAATCAGCCTGCTACCTCCGCCAGGGATACCAGGAAATGATGAAGG TGCACACTGTCCCATGGGATGGCAAGAAACGGGTCTGGGTGCCTGATGAACAGGATGCCTATGTCGAAGC TGAGGTCAAGACTGAAGCTACTGGAGGCCGAGTCACTGTGGAGACCAAAGACCAGAAGGTGCTGACAGTG CGTGAAACGGAAATGCAGCCCATGAACCCACCCCGCTTCGACTTACTGGAGGACATGGCCATGATGACGC ACCTCAACGAGGCTGCAGTGCTGCACAACCTGCGCCAGCGTTATGCTCGCTGGATGATCTACACATACTC GGGCCTCTTCTGTGTCACCATTAACCCATACAAATGGCTCCCGGTCTACACAGCTACTGTGGTGGCCGCC TACAAGGGCAAGCGCCGCTCGGAGGCCCCGCCTCATATATATGCAGTGGCAGATAATGCCTACAATGACA TGCTCCGCAACAGAGAGAACCAGTCCATGCTGATCACCGGAGAGTCGGGGGCCGGTAAGACGGTTAACAC CAAGCGGGTCATTCAGTATTTTGCCATCGTCGCTGCCCTGGGAGACGGGCCGGGCAAGAAGGCACAATTT CTGGCAACAAAAACCGGGGGCACCCTTGAGGATCAAATCATCGAGGCTAACCCTGCCATGGAGGCTTTTG GTAACGCCAAGACTCTGAGGAACGACAACTCCTCCCGTTTTGGCAAGTTCATCCGCATCCACTTTGGTCC CACTGGGAAGCTGGCCTCTGCGGACATTGACAGCTATCTCCTGGAAAAGTCTCGAGTGATCTTCCAGCTG CCCGGTGAGCGTGGTTACCATGTCTACTACCAAATCCTCTCCGGGAAGAAGCCAGAGCTGCAGGACATGC TGCTCCTCTCCATGAACCCCTACGACTACCACTTCTGCAGTCAGGGGGTCACCACCGTGGATAACATGAA CGACGGGGAGGAGCTTATCGCCACTGATCATGCAATGGACATCCTGGGCTTCAGCGTGGATGAGAAGTGC GCATGCTATAAGATCGTGGGCGCCCTCCTGCACTTTGGCAACATGAAGTTCAAACAGAAGCAGAGAGAGG AACAGGCTGAGGCAGACGGCACTGAGAGTGCCGACAAGGCTGCCTACCTGATGGGAGTCAGCAGTGGAGA CCTCCTCAAAGGTCTTCTGCACCCCCGGGTTCGTGTGGGAAACGAGTATGTGACCAAGGGCCAGAGTGTG GAGCAGGTGGTATTTGCTGTGGGGGCCCTGGCCAAGGCTACCTATGACCGGTTGTTCCGATGGCTGGTAT CTCGAATCAATCAGACCCTGGACACAAAGCTCCCCCGTCAGTTCTTCATTGGGGTCCTGGATATCGCTGG TTTTGAGATCTTTGAGTTTAACAGCTTCGAGCAACTGTGTATCAACTTCACCAACGAGAAACTGCAGCAG TTCTTCAACCAGCACATGTTCGTGCTGGAGCAGGAGGAGTACAAACGGGAGGGCATTGACTGGGTCTTCA TCGACTTCGGCCTTGACCTGCAGCCCTGCATCGACCTCATCGAGAAGCCTCTGGGCATTCTGTCCATCCT GGAGGAGGAGTGCATGTTCCCCAAGGCTTCAGACGCAAGCTTCAGGGCCAAGCTCTATGACAATCACTCT GGGAAGTCACCTAATTTCCAGCAGCCTCGCCCAGACAAGAAACGCAAGTACCAGGCTCACTTCGAGGTGG TCCACTACGCAGGCGTGGTACCTTATAGCATTGTGGGCTGGCTGGAGAAAAACAAGGACCCCCTCAATGA GACCGTGGTCCCCATCTTCCAAAAGTCACAGAATAGGCTCTTGGCCACCCTCTATGAGAACTATGCAGGT TCTTGCTCCACCGAGCCCCCCAAGTCTGGGGTGAAAGAGAAACGAAAGAAAGCAGCATCATTTCAGACAG TGTCTCAGCTTCACAAGGAGAACCTCAACAAGCTTATGACCAACCTGCGGGCCACACAGCCCCACTTCGT CCGTTGTATCGTCCCCAACGAGAACAAGACTCCAGGGGTCATGGATTCCTTCTTGGTGCTACACCAGCTA CGCTGTAACGGGGTCCTAGAGGGGATCCGGATCTGCCGCCAAGGGTTCCCCAACAGACTGCTGTATGCTG ACTTCAGGCAGCGGTACCGAATCCTGAACCCCAGTGCCATCCCAGATGACACCTTTGTGGACAGCAGGAA GGCCACAGAGAAGCTGCTGGGCTCTCTGGACATTGACCACACCCAGTATCAGTTTGGCCACACCAAGGTG TTCTTCAAGGCTGGGCTTCTAGGCATCCTGGAGGAGCTCCGGGACCAGCGTCTGGCTAAGGTGCTGACAC TGCTGCAGGCTCGGAGCCGGGGCCGCCTCATGCGTCTGGAGTACCAGCGCATGCTAGGAGGAAGGGATGC CCTGTTCACCATCCAGTGGAACATCCGGGCCTTCAATGCCGTCAAGAACTGGTCCTGGATGAAGCTCTTC TTCAAGATGAAGCCACTGCTGCGCTCAGCACAGGCAGAGGAAGAGCTGGCAGCACTTCGGGCAGAACTGC GGGGGCTGCGGGGGGCCCTGGCTACGGCTGAGGCCAAGCGTCAGGAGCTGGAGGAGACTCAAGTCAGTGT CACTCAGGAGAAGAATGACCTGGCCCTGCAGCTGCAGGCAGAGCAGGACAACCTAGCGGATGCGGAGGAG CGCTGCCACTTGCTCATCAAGTCCAAGGTGCAGCTCGAGGCGAAGGTGAAGGAGCTGAACGAGCGGCTGG AGGATGAGGAGGAAGTGAACGCTGACCTGGCCGCACGCAGGCGCAAGCTGGAGGATGAGTGCACGGAGCT CAAGAAGGACATTGATGACCTGGAGCTGACCCTGGCCAAGGCTGAGAAGGAGAAACAAGCCACCGAGAAT AAGGTGAAGAACCTGACAGAGGAGATGGCTGCCCTGGACGAGTCCGTGGTCCGCCTGACTAAGGAGAAGA AGGCGTTGCAGGAGGCTCACCAGCAGGCTCTGGGTGACCTGCAGGCTGAAGAGGACCGTGTGAGTGCGCT GGCCAAGGCCAAGATCCGGTTGGAGCAGCAAGTAGAGGACCTGGAGTGCTCCCTGGAGCAAGAGAAGAAG CTTCGCATGGATACAGAACGTGCCAAGCGCAAGCTCGAAGGAGACCTGAAGCTGACGCAGGAGACAGTGA CAGACACCACACAAGACAAGCAGCAGCTGGAGGAGAAGTTGAAGAAGAAGGATTCAGAGCTGAGTCAGCT GAGCCTGCGGGTGGAGGATGAGCAGCTCCTGGGGGCGCAGTTGCAGAAGAAGATCAAGGAGCTACAGGCG AGGGCTGAGGAGCTGGAGGAGGAGCTGGAGGCGGAGCGAGCAGCCCGTGCCCGTGTGGAAAAGCAGCGAG CTGAGGCAGCCCGAGAGCTGGAGGAGCTGAGTGAGAGGCTAGAGGAGGCTGGTGGAGCCTCTGCCGGGCA GCGTGAGGGTTGCCGCAAGCGTGAGGCTGAGTTGGGCCGACTGCGGCGGGAGCTGGAGGAGACAGTTCTG AGACACGAGGCCACTGTGGCTGCCCTGCGCCGCAAGCAGGCTGATAGCGCCGCAGAGTTGAGCGAGCAGG TAGACAGCCTCCAGCGGATCCGGCAGAAACTGGAGAAGGAAAAGAGTGAGCTGCGCATGGAGGTGGATGA TCTGGGGGCCAGCGTGGAAACTCTGGCCCGTGGCAAGGCCAGTGCAGAGAAGCTGTGCCGGACCTATGAG GACCAGCTCAGCGAGGCCAAGATCAAAGTGGAGGAGCTGCAGCGGCAGCTGACTGACGCCAGCACCCAGC GGGGGCGGTTACAAACGGAGAACGGGGAGCTGGGCCGCCTGCTTGAGGAGAAGGAGTCTATGATCAGCCA GCTGAGCCGCGGGAAGACCTCAGCCGCTCAGAGTCTGGAGGAACTTCGGCGGCAGTTGGAGGAAGAAAGC AAGGCCAAGGGTGCGCTGGCTCACGCAGTGCAGGCTCTGCGGCACGACTGCGACCTCCTGCGAGAGCAGC ATGAGGAAGAGTCCGAGGCGCAGGCTGAGCTGCAGCGGCTGCTGTCCAAAGCCAACGCTGAGGTGGCACA GTGGAGGAGCAAGTATGAGGCGGACGCCATCCAAAGGACGGAGGAGCTGGAGGAGGCCAAGAAGAAGCTG GCCCTGAGGCTGCAGGAGGCAGAAGAAGGCGTAGAGGCTGCGAATGCCAAGTGCTCCTCACTGGAGAAGG CCAAGCTGCGGCTGCAGACAGAGTCCGAGGACGTGACCTTGGAGCTGGAGCGGGCCACCTCAGCCGCTGC TGCACTGGATAAGAAGCAGCGGCACTTGGAGCGGGCCCTGGAGGAACGGAGGCGGCAGGAGGAGGAGATG CAGCGGGAACTGGAGGCGGCGCAGAGGGAGGCTCGAGGTCTGGGCACCGAGCTCTTTCGATTGCGGCACA GCCATGAGGAGGCGCTTGAGGCTCTGGAGACACTCAAGCGGGAGAACAAGAATCTGCAGGAGGAGATCAG CGACCTTACAGACCAGGTCAGCCTCAGCGGGAAGAGCATCCAGGAACTGGAGAAGGCCAAGAAGGCGCTG GAAGGGGAGAAGAGTGAGCTGCAGGCTGCGCTGGAGGAGGCTGAGGGGGCGCTGGAGCTGGAAGAGACAA AGACCCTGCGGATCCAGTTGGAGTTGTCCCAGGTCAAGGCGGAAGTGGACCGAAAGCTGGCGGAGAAGGA TGAGGAGTGTACTAACCTGAGGCGCAACCACCAGCGGGCTGTGGAGTCCTTGCAGGCCTCCCTGGATGCC GAGACTAGGGCCCGCAATGAAGCACTGCGTCTCAAGAAGAAGATGGAGGGGGACCTCAATGACCTGGAGT TACAGCTGGGCCACGCAACCCGCCAGGCCATGGAGGCACAGGCAGCCACGAGGCTGCTTCAGGCCCAACT CAAGGAGGAGCAAGCAGGGCGTGATGAGGAGCAGAGGCTGGCTGCCGAGCTCCGCGAGCAGGGCCAGGCC CTCGAGCGCAGGGCTGCACTGCTGGCATCTGAGCTGGAAGAGCTTCGGGCCGCCCTGGAACAGGGCGAGC GCAGCCGAAGGCTGGCTGAACAGGAGCTGTTGGAGGCCACAGAGCGCCTCAACCTACTGCATTCACAGAA CACAGGCCTCCTGAACCAGAAAAAGAAGCTGGAGGTGGACTTGGCCCAGCTGAGTGGGGAGGTGGAGGAG GCTGCGCAGGAGAGGCGGGAAGCTGAGGAGAAGGCCAAAAAGGCCATCACGGATGCAGCCATGATGGCTG AGGAGCTGAAAAAGGAGCAGGACACAAGTGCACACCTCGAACGGATGAAGAAGACCCTGGAACAGACAGT GCGGGAGCTGCAGGCCCGCCTTGAGGAAGCAGAGCAGGCCGCACTCCGGGGTGGGAAGAAGCAAGTGCAG AAGCTGGAAGCCAAGGTGCGGGAACTGGAGGCAGAGCTTGACGCAGAGCAGAAGAAGCACGCTGAGGCCC TCAAGGGTGTGCGCAAACATGAGCGCAGGGTCAAGGAGCTTGCGTACCAGACTGAGGAAGACAGGAAGAA CCTGGCTCGCATGCAGGACCTAGTGGACAAGCTGCAGAGCAAGGTCAAGAGCTACAAGCGTCAGTTTGAG GAGGCGGAGCAGCAGGCCAGTACCAACCTGGCTAAGTACCGCAAGGCCCAGCACGAGTTAGACGACGCTG AGGAGCGGGCAGACATGGCAGAGACCCAGGCCAACAAGCTGCGGGCAAGGTCCAGGGATGCCCTGGGTCC CAAGCACAAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RR204114 representing NM_001107794 Red=Cloning site Green=Tags(s) MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTV RETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAA YKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQF LATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQL PGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKC ACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSV EQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQ FFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHS GKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAG SCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQL RCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKV FFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLF FKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEE RCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATEN KVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKK LRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQA RAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVL RHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYE DQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEES KAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKL ALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEM QRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKAL EGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDA ETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQA LERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEE AAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQ KLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFE EAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKHKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001107794
ORF Size	5823 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001107794.2, NP_001101264.2
RefSeq Size	6097 bp
RefSeq ORF	5826 bp
Locus ID	311570
MW	221.6 kDa

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