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Cyp7b1 (NM_019138) Rat Tagged ORF Clone
CAT＃: RR204118
- TrueORF®
Cyp7b1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10 ug)
"NM_019138" in other vectors (3)
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CNY 6,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204118 representing NM_019138
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGAGCCACGACCCCAGATGCCGCTTCGCCCGGGCCTCTCTCCCTACTAGGCCTTCTCTTTGCGG TCACCTTGCTGCTCCCAGTCCTGTTCCTCCTCACCCGGCGCACAAGGCGTCCTTGTGAACCTCCCTTGAT AAAAGGTTGGATTCCTTATCTTGGCATGGCCCTGAAATTCTGGAAGGATCCGTTAGCTTTCTTGCAAACT CTTCAAAGGCAATGTGGTGACACTTTCACTGTCTTACTTGGAGGGAAGTATATAACATTTGTTCTGAACC CTTTCCAGTACCAGTATGTAATGAAAAACCCAAAACAATTAAGCTTTGAGAAGTTCAGCCGAAGATTATC AGCGAAAGCCTTCTCTGTCAAGAAGCTGCTAACTAATGACGACCTTAGCAATGACATTCACAGAGGCTAT CTTCTTTTACAAGGCAAATCTCTGGATGGTCTTCTGGAAACCATGATCCAAGAAGTAAAAGAAATATTTG AGTCCAGACTGCTAAAACTCACAGATTGGAATACAGCAAGAGTATTTGATTTCTGTAGTTCACTGGTATT TGAAATCACATTTACAACTATATATGGAAAAATTCTTGCTGCTAACAAAAAACAAATTATCAGTGAGCTG AGGGATGATTTTTTAAAATTTGATGACCATTTCCCATACTTAGTATCTGACATACCTATTCAGCTTCTAA GAAATGCAGAATTTATGCAGAAGAAAATTATAAAATGTCTCACACCAGAAAAAGTAGCTCAGATGCAAAG ACGGTCAGAAATTGTTCAGGAGAGGCAGGAGATGCTGAAAAAATACTACGGGCATGAAGAGTTTGAAATA GGAGCACATCATCTTGGCTTGCTCTGGGCCTCTCTAGCAAACACCATTCCAGCTATGTTCTGGGCAATGT ATTATCTTCTTCAGCATCCAGAAGCTATGGAAGTCCTGCGTGACGAAATTGACAGCTTCCTGCAGTCAAC AGGTCAAAAGAAAGGACCTGGAATTTCTGTCCACTTCACCAGAGAACAATTGGACAGCTTGGTCTGCCTG GAAAGCGCTATTCTTGAGGTTCTGAGGTTGTGCTCCTACTCCAGCATCATCCGTGAAGTGCAAGAGGATA TGGATTTCAGCTCAGAGAGTAGGAGCTACCGTCTGCGGAAAGGAGACTTTGTAGCTGTCTTTCCTCCAAT GATACACAATGACCCAGAAGTCTTCGATGCTCCAAAGGACTTTAGGTTTGATCGCTTCGTAGAAGATGGT AAGAAGAAAACAACGTTTTTCAAAGGAGGAAAAAAGCTGAAGAGTTACATTATACCATTTGGACTTGGAA CAAGCAAATGTCCAGGCAGATACTTTGCAATTAATGAAATGAAGCTACTAGTGATTATACTTTTAACTTA TTTTGATTTAGAAGTCATTGACACTAAGCCTATAGGACTAAACCACAGTCGCATGTTTCTGGGCATTCAG CATCCAGACTCTGACATCTCATTTAGGTACAAGGCAAAATCTTGGAGATCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204118 representing NM_019138
Red=Cloning site Green=Tags(s) MEGATTPDAASPGPLSLLGLLFAVTLLLPVLFLLTRRTRRPCEPPLIKGWIPYLGMALKFWKDPLAFLQT LQRQCGDTFTVLLGGKYITFVLNPFQYQYVMKNPKQLSFEKFSRRLSAKAFSVKKLLTNDDLSNDIHRGY LLLQGKSLDGLLETMIQEVKEIFESRLLKLTDWNTARVFDFCSSLVFEITFTTIYGKILAANKKQIISEL RDDFLKFDDHFPYLVSDIPIQLLRNAEFMQKKIIKCLTPEKVAQMQRRSEIVQERQEMLKKYYGHEEFEI GAHHLGLLWASLANTIPAMFWAMYYLLQHPEAMEVLRDEIDSFLQSTGQKKGPGISVHFTREQLDSLVCL ESAILEVLRLCSYSSIIREVQEDMDFSSESRSYRLRKGDFVAVFPPMIHNDPEVFDAPKDFRFDRFVEDG KKKTTFFKGGKKLKSYIIPFGLGTSKCPGRYFAINEMKLLVIILLTYFDLEVIDTKPIGLNHSRMFLGIQ HPDSDISFRYKAKSWRS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019138 |
| ORF Size | 1521 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019138.1, NP_062011.1 |
| RefSeq Size | 2626 bp |
| RefSeq ORF | 1524 bp |
| Locus ID | 25429 |
| UniProt ID | Q63688 |
| MW | 58.6 kDa |
| Gene Summary | member of the p450 subfamily of cholesterol metabolizing enzymes that performs 7alpha-hydroxylation of 27-hydroxycholesterol in a bile synthesis pathway alternative to the classical cholesterol disposal pathway [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204118 | Cyp7b1 (untagged ORF) - Rat cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10 ug) |
CNY 6,560.00 |
|
| RR204118L3 | Lenti ORF clone of Cyp7b1 (Myc-DDK-tagged ORF) - Rat cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10 ug) |
CNY 8,460.00 |
|
| RR204118L4 | Lenti ORF clone of Cyp7b1 (mGFP-tagged ORF) - Rat cytochrome P450, family 7, subfamily b, polypeptide 1 (Cyp7b1), (10 ug) |
CNY 9,220.00 |
