Pla2g4a (NM_133551) Rat Tagged ORF Clone
CAT#: RR204286
- TrueORF®
Pla2g4a (Myc-DDK-tagged ORF) - Rat phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10 ug)
"NM_133551" in other vectors (3)
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CNY 9,690.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | cPLA2; Pla2c; Pla2g4 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204286 representing NM_133551
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTCATAGATCCTTATCAGCACATAATAGTGGAACACCAGTATTCCCATAAGTTTACAGTAGTGG TTCTACGTGCCACCAAAGTAACCAAGGGGACCTTTGGCGATATGCTGGACACTCCTGATCCTTATGTGGA ACTTTTCATCTCTACAACCCCTGACAGCAGGAAGCGAACAAGACACTTCAATAATGATATAAACCCTGTG TGGAATGAGACCTTTGAGTTCATTTTGGATCCTAATCAGGAAAATGTTTTGGAGATCACATTGATGGATG CCAATTACGTCATGGATGAAACCCTAGGCACAGCTACATTTCCTGTATCTTCTATGAAGGTGGGAGAGAA GAAAGAAGTCCCTTTTATTTTCAACCAAGTCACAGAAATGATTCTGGAAATGTCTCTTGAAGTTTGCTCA TGCCCAGACCTACGGTTCAGCATGGCACTGTGTGATCAGGAGAAAACATTCAGGCAGCAGAGGAAAGAGA ACATAAAAGAGAACATGAAGAAACTTTTGGGTCCAAAAAAGAGCGAGGGGCTTTATTCCACACGTGATGT GCCTGTGGTGGCCATTTTGGGTTCCGGCGGGGGTTTCCGGGCCATGGTGGGATTCTCCGGTGTGATGAAG GCGCTCTATGAATCAGGGATTTTGGATTGTGCGACCTACGTTGCTGGTCTGTCCGGCTCCACATGGTACA TGTCAACCTTGTACTCCCACCCTGATTTTCCAGAGAAAGGTCCTGAGGAGATTAATGAAGAGCTAATGAA AAATGTTAGCCACAACCCTCTCTTACTTCTTACGCCACAGAAAGTTAAAAGATATGTTGAGTCTTTATGG AAGAAGAAAAGTTCTGGCCAACCTGTCACCTTTACTGACATCTTTGGAATGTTAATAGGAGAAACACTAA TTCAAAATAGAATGAGTACGACCTTGAGTAGCTTGAAGGAAAAGGTCAGCGCCGCCCGGTGTCCTCTGCC TCTCTTCACCTGTCTCCATGTCAAACCGGACGTGTCAGAGCTGATGTTTGCCGATTGGGTAGAATTTAGT CCATACGAAATTGGCATGGCAAAATATGGTACCTTTATGACTCCTGACCTGTTTGGAAGCAAATTTTTTA TGGGAACAGTTGTAAAAAAATATGAAGAAAACCCCTTGCATTTCTTAATGGGTGTCTGGGGCAGTGCCTT TTCTATACTGTTCAACAGAGTTTTGGGAGTTTCTGGCTCACAGAATAAAGGTTCTACAATGGAGGAGGAA TTAGAAAATATTACAGCAAAGCACATTGTGAGTAACGACAGCTCTGACAGCGATGACGAGGCCCAAGGAC CCAAAGGCACCGAGAATGAAGATGCGGAAAGAGAGTACCAAAATGACAACCAAGCAAGTTGGGTCCATCG GATGCTAATGGCCTTGGTGAGTGACTCAGCTTTATTCAATACCCGAGAAGGACGTGCTGGGAAGGTGCAT AACTTCATGTTGGGCTTGAATCTCAACACATCGTATCCACTGTCTCCCCTGAGAGACTTCAGCCCCCAAG ATTCCTTCGATGATGATGAACTCGACGCAGCGGTAGCAGATCCAGATGAATTTGAACGAATATATGAACC ACTGGATGTCAAAAGTAAAAAGATTCATGTTGTAGACAGTGGGCTCACGTTTAACCTGCCGTATCCCTTG ATTCTGCGACCTCAGAGAGGTGTGGATCTCATCATTTCCTTTGACTTTTCTGCAAGGCCAAGTGACACCA GCCCTCCATTCAAGGAACTTCTGCTTGCAGAGAAGTGGGCTAAAATGAACAAGCTCCCTTTTCCAAAGAT TGATCCTTACGTGTTTGATCGGGAAGGATTGAAGGAATGCTATGTGTTTAAACCTAAGAATCCTGATGTG GAGAAGGATTGCCCAACCATTATCCACTTTGTTCTGGCCAACATCAACTTCAGAAAGTACAAGGCCCCAG GTGTTCTGAGGGAAACCAAGGAAGAGAAAGAAATAGCTGACTTTGACATTTTCGATGACCCCGAATCGCC ATTTTCAACCTTCAACTTCCAGTATCCAAATCAAGCATTCAAAAGGCTACATGATCTGATGTACTTCAAC ACACTGAACAACATTGATGTGATAAAGGATGCCATTGTTGAGAGCATTGAATACAGAAGACAGAACCCAT CTCGTTGCTCTGTTTCCCTCAGTAATGTTGAGGCAAGAAAATTCTTCAACAAGGAGTTCCTAAGTAAACC CACAGCAGAGTCCATT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204286 representing NM_133551
Red=Cloning site Green=Tags(s) MSFIDPYQHIIVEHQYSHKFTVVVLRATKVTKGTFGDMLDTPDPYVELFISTTPDSRKRTRHFNNDINPV WNETFEFILDPNQENVLEITLMDANYVMDETLGTATFPVSSMKVGEKKEVPFIFNQVTEMILEMSLEVCS CPDLRFSMALCDQEKTFRQQRKENIKENMKKLLGPKKSEGLYSTRDVPVVAILGSGGGFRAMVGFSGVMK ALYESGILDCATYVAGLSGSTWYMSTLYSHPDFPEKGPEEINEELMKNVSHNPLLLLTPQKVKRYVESLW KKKSSGQPVTFTDIFGMLIGETLIQNRMSTTLSSLKEKVSAARCPLPLFTCLHVKPDVSELMFADWVEFS PYEIGMAKYGTFMTPDLFGSKFFMGTVVKKYEENPLHFLMGVWGSAFSILFNRVLGVSGSQNKGSTMEEE LENITAKHIVSNDSSDSDDEAQGPKGTENEDAEREYQNDNQASWVHRMLMALVSDSALFNTREGRAGKVH NFMLGLNLNTSYPLSPLRDFSPQDSFDDDELDAAVADPDEFERIYEPLDVKSKKIHVVDSGLTFNLPYPL ILRPQRGVDLIISFDFSARPSDTSPPFKELLLAEKWAKMNKLPFPKIDPYVFDREGLKECYVFKPKNPDV EKDCPTIIHFVLANINFRKYKAPGVLRETKEEKEIADFDIFDDPESPFSTFNFQYPNQAFKRLHDLMYFN TLNNIDVIKDAIVESIEYRRQNPSRCSVSLSNVEARKFFNKEFLSKPTAESI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_133551 |
ORF Size | 2256 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133551.2, NP_598235.2 |
RefSeq Size | 2855 bp |
RefSeq ORF | 2259 bp |
Locus ID | 24653 |
MW | 85.6 kDa |
Gene Summary | catalyzes the synthesis of COX-2 substrate arachidonic acid (AA); may play a role in epileptogenesis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204286 | Pla2g4a (untagged ORF) - Rat phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10 ug) |
CNY 9,690.00 |
|
RR204286L3 | Lenti ORF clone of Pla2g4a (Myc-DDK-tagged ORF) - Rat phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10 ug) |
CNY 11,500.00 |
|
RR204286L4 | Lenti ORF clone of Pla2g4a (mGFP-tagged ORF) - Rat phospholipase A2, group IVA (cytosolic, calcium-dependent) (Pla2g4a), (10 ug) |
CNY 12,540.00 |
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