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Dagla (NM_001005886) Rat Tagged ORF Clone
CAT＃: RR204355
- TrueORF®
Dagla (Myc-DDK-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug)
"NM_001005886" in other vectors (3)
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CNY 16,820.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Nsddr |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR204355 representing NM_001005886
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGGGATCGTGGTGTTCCGGCGGCGCTGGTCTGTGGGCAGCGATGACCTCGTGCTGCCTGCTATCT TCCTCTTCCTGCTGCACACCACCTGGTTTGTGATCCTGTCTGTGGTGCTCTTCGGCCTGGTCTACAACCC GCACGAGGCCTGCTCCCTGAACCTGGTGGACCACGGCCGAGGCTACCTGGGCATCCTTCTGAGCTGTATG ATTGCTGAGATGGCTATCATTTGGCTGAGCATGCGTGGGGGTATCCTCTACACAGAACCCCGGGACTCCA TGCAGTACGTGCTCTACGTGCGCCTGGCCATTTTGGTGATCGAGTTCATCTACGCTATCGTGGGCATCGT CTGGCTTACACAGTACTACACCTCCTGCAATGACCTCACTGCTAAGAATGTCACTCTCGGGATGGTCGTC TGCAACTGGGTGGTCATCCTCAGCGTCTGCATCACTGTCCTCTGTGTCTTTGACCCCACTGGCCGCACCT TCGTCAAGCTGAGAGCCACCAAGAGGAGGCAGCGCAACCTGCGGACTTACAACCTGCGGCATCGGTTAGA GGAGGGTCAGGCCACCAGCTGGTCCCGCCGTCTAAAAGTGTTCCTCTGTTGCACCCGGACAAAGGATTCC CAGTCAGATGCCTATTCAGAAATTGCCTACCTCTTTGCTGAATTTTTCCGTGACCTCGACATAGTGCCCT CCGACATCATTGCTGGCCTGGTGCTGCTTAGGCAGCGGCAGCGAGCCAAGCGCAATGCGGTGCTGGATGA GGCAAACAATGACATCTTGGCTTTCCTGTCTGGGATGCCAGTGACCAGAAACACCAAGTACCTCGACCTC AAGAACTCGCACGAGATGCTACGCTACAAAGAAGTCTGCTACTACATGCTCTTTGCCCTCGCTGCCTATG GCTGGCCCATGTACCTGATGAGGAAGCCCACCTGTGGCCTCTGCCAGCTGGCTCGGTCCTGCTCGTGTTG TCTCTGCCCTGCACGCCCCCGATTCGCCCCTGGGGTCACCATTGAGGAAGACAACTGTTGTGGCTGCAAT GCAATTGCCATCCGCCGTCACTTCCTGGATGAGAACATGACTGCTGTAGACATCGTCTATACCTCCTGTC ATGATGCGGTCTATGAAACTCCCTTCTACGTAGCTGTGGACCATGACAAGAAGAAGGTGGTGATCAGTAT CCGGGGAACACTGTCCCCCAAGGATGCCCTGACTGACCTGACAGGAGATGCTGAGCGTCTCCCTGTGGAG GGACACCGAGGCACCTGGTTGGGCCACAAGGGCATGGTACTCTCAGCTGAGTACATCAAGAAGAAGCTGG AGCAGGAGATGGTCCTGTCCCAGGCCTTTGGGCGAGACCTGGGCCGTGGAACCAAACACTATGGCCTGAT TGTGGTAGGCCACTCCTTGGGAGCAGGCACAGCCGCCATTCTCTCCTTCCTCCTGCGCCCTCAGTACCCG ACCCTCAAGTGCTTCGCCTACTCCCCTCCAGGAGGCCTGCTGAGTGAGGATGCCATGGAGTACTCCAAAG AGTTTGTGACTGCTGTGGTTCTGGGCAAAGACCTTGTTCCCAGGATCGGCCTTTCCCAGCTCGAAGGTTT CCGCCGGCAGCTTCTGGATGTCCTGCAGCGAAGCACCAAGCCCAAATGGCGGATCATCGTGGGGGCCACC AAGTGTATCCCCAAGTCGGAGCTGCCTGAGGATCAGGTGGAAGTGACTGCCTTGGCCAGCACACGGCTCT GGACACACCCCAGTGATCTGACCATCGCCCTTTCAGCCAGCACCCCACTCTACCCACCTGGCCGTATCAT CCATGTGGTCCATAACCACCCTGCTGAACAGTGCTGCTGCTGTGAACAGGAAGAGCCCACATACTTTGCC ATCTGGGGTGACAACAAAGCCTTTAATGAGGTGATCATCTCACCAGCCATGCTCCATGAGCACCTGCCCT ACGTGGTCATGGAGGGGCTCAATAAGGTGCTGGAGAATTACAACAAGGGAAAGACAGCGCTGCTCTCTGC TGCAAAGGTCATGGTGAGCCCCACTGAGGTAGACCTCACTCCTGAGCTCATCTTCCAGCAACAGCCGCTG CCCACGGGGCCACCTCTGCCCACTGGGCTGGCTCTGGAGCTGCCTGCCACAGAGCATCGCAACAGCAGTG TCAGGAGCAAGTCTCAGTCTGAGATGAGTCTAGAGGGCTTCTCTGAAGGACGGCTGCTGTCCCCAGTGGC TGCTGCCTCAGCAGCCCGGCAAGACCCTGTAGAGCTGTTGCTGCTGTCCACCCAGGAACGACTGGCCGCG GAGCTGCAGTCCCGGCGGGCACCACTGGCCACTATGGAGAGCCTCTCAGACACTGAGTCACTGTACAGCT TTGACTCACGCCGTTCCTCAGGCTTCCGCAGCATCAGAGGCTCACCCAGCCTCCATGCAGTACTGGAGCG TGATGAGGGCCACCTGTTTTACATCGACCCAGCCATTCCAGAGGAGAACCCATCCCTCAGCTCCCGCACA GAGCTGCTGGCAGCTGACAGTCTGTCCAAGCACTCGCAGGACACCCAACCCCTGGAGGCAGCACTAGGCA GCGGGGGTGTGACTCCTGAGCGACCACCAAGTGCAGCCAATGACGAGGAGGAGGCAGCCGGTGGCAGTGA GGGTGGTGGGGTGGCCCCACGAGGAGAGCTGGCACTGCACAATGGGCGCCTGGGGGATTCGCCCAGCCCT CAGGTGCTAGAATTCGCTGAGTTCATTGACAGCCTCTTCAACCTGGACAGCAAGAGCAGCTCCTTCCAGG ACCTGTACTGCATGATGGTGCCTGAGAGCCCCACTAGTGACTACACAGAGGGCCCCAAGTCCCCCAGCCA ACAGGAAATCTTGCTTCGGGCTCAGTTTGAGCCCAATCTGGTACCCAAGCCCCCCAGGCTCTTTGCAGGA TCTGCCGAACCCTCCTCTGGCATCTCACTCTCACCCTCCTTCCCACTCAGCTCCTCAGGGGAGCTCATGG ACCTGACACCCACGGGCCTCAGCAGCCAGGAGTGTCTGGCCACAGACAAGATCCGGACTTCCACCCCCAC AGGCCATGGGGCCAGCCCTACCAAGCAGGATGACCTGGTCATCTCAGCACGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204355 representing NM_001005886
Red=Cloning site Green=Tags(s) MPGIVVFRRRWSVGSDDLVLPAIFLFLLHTTWFVILSVVLFGLVYNPHEACSLNLVDHGRGYLGILLSCM IAEMAIIWLSMRGGILYTEPRDSMQYVLYVRLAILVIEFIYAIVGIVWLTQYYTSCNDLTAKNVTLGMVV CNWVVILSVCITVLCVFDPTGRTFVKLRATKRRQRNLRTYNLRHRLEEGQATSWSRRLKVFLCCTRTKDS QSDAYSEIAYLFAEFFRDLDIVPSDIIAGLVLLRQRQRAKRNAVLDEANNDILAFLSGMPVTRNTKYLDL KNSHEMLRYKEVCYYMLFALAAYGWPMYLMRKPTCGLCQLARSCSCCLCPARPRFAPGVTIEEDNCCGCN AIAIRRHFLDENMTAVDIVYTSCHDAVYETPFYVAVDHDKKKVVISIRGTLSPKDALTDLTGDAERLPVE GHRGTWLGHKGMVLSAEYIKKKLEQEMVLSQAFGRDLGRGTKHYGLIVVGHSLGAGTAAILSFLLRPQYP TLKCFAYSPPGGLLSEDAMEYSKEFVTAVVLGKDLVPRIGLSQLEGFRRQLLDVLQRSTKPKWRIIVGAT KCIPKSELPEDQVEVTALASTRLWTHPSDLTIALSASTPLYPPGRIIHVVHNHPAEQCCCCEQEEPTYFA IWGDNKAFNEVIISPAMLHEHLPYVVMEGLNKVLENYNKGKTALLSAAKVMVSPTEVDLTPELIFQQQPL PTGPPLPTGLALELPATEHRNSSVRSKSQSEMSLEGFSEGRLLSPVAAASAARQDPVELLLLSTQERLAA ELQSRRAPLATMESLSDTESLYSFDSRRSSGFRSIRGSPSLHAVLERDEGHLFYIDPAIPEENPSLSSRT ELLAADSLSKHSQDTQPLEAALGSGGVTPERPPSAANDEEEAAGGSEGGGVAPRGELALHNGRLGDSPSP QVLEFAEFIDSLFNLDSKSSSFQDLYCMMVPESPTSDYTEGPKSPSQQEILLRAQFEPNLVPKPPRLFAG SAEPSSGISLSPSFPLSSSGELMDLTPTGLSSQECLATDKIRTSTPTGHGASPTKQDDLVISAR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001005886 |
| ORF Size | 3132 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001005886.1, NP_001005886.1 |
| RefSeq Size | 5568 bp |
| RefSeq ORF | 3135 bp |
| Locus ID | 309207 |
| UniProt ID | Q5YLM1 |
| MW | 115.3 kDa |
| Gene Summary | Catalyzes the hydrolysis of diacylglycerol (DAG) to 2-arachidonoyl-glycerol (2-AG), the most abundant endocannabinoid in tissues. Required for axonal growth during development and for retrograde synaptic signaling at mature synapses (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN204355 | Dagla (untagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug) |
CNY 16,820.00 |
|
| RR204355L3 | Lenti ORF clone of Dagla (Myc-DDK-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug) |
CNY 18,430.00 |
|
| RR204355L4 | Lenti ORF clone of Dagla (mGFP-tagged ORF) - Rat diacylglycerol lipase, alpha (Dagla), (10 ug) |
CNY 20,140.00 |
