Gpr4 (NM_001025680) Rat Tagged ORF Clone
CAT#: RR204537
- TrueORF®
Gpr4 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 4 (Gpr4), (10 ug)
"NM_001025680" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | MGC116369 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204537 representing NM_001025680
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAACAGCACGGGCACGTGGGAGGGCTGCCATGTGGACTCTCGAGTGGACCACCTCTTCCCACCAT CCCTCTACATCTTCGTCATCGGGGTGGGGCTGCCCACCAACTGCCTGGCCCTGTGGGCAGCCTACCGCCA GGTGCGCCAGCGCAATGAGCTGGGCGTCTACCTGATGAACTTGAGCATCGCAGACCTGCTGTACATCTGT ACGCTGCCGCTGTGGGTCGACTACTTCCTCCACCATGACAACTGGATCCACGGCCCCGGCTCCTGCAAGC TCTTTGGCTTCATCTTCTACAGCAACATCTACATCAGCATCGCCTTCCTGTGCTGCATCTCCGTGGACCG CTACCTGGCTGTGGCCCATCCGCTGCGCTTTGCGCGCCTGCGCCGGGTCAAGACAGCAGTAGCTGTGAGC TCCGTGGTCTGGGCCACCGAGCTAGGCGCCAACTCGGCACCGCTCTTTCATGACGAGCTCTTTCGTGATC GCTACAACCACACCTTCTGCTTCGAGAAGTTCCCCATGGAGCGCTGGGTGGCCTGGATGAACCTGTACCG CGTCTTTGTGGGGTTCCTCTTCCCCTGGGCACTCATGTTGCTGTGCTACCGCGGCATCCTGCGGGCCGTA CAGAGCAGTGTGTCCACCGAGCGCCAGGAGAAAGTCAAGATCAAACGCCTGGCCCTGAGCCTCATCGCCA TCGTGCTGGTGTGCTTTGCACCCTACCATGCTCTCTTGCTGTCTCGCAGCGCTGTCTATCTGGGCCGGCC CTGGGACTGTGGCTTCGAGGAGCGAGTCTTCTCTGCCTACCACAGCTCCCTAGCCTTCACCAGCCTCAAT TGCGTGGCTGACCCCATCCTCTACTGCCTGGTCAACGAGGGTGCCCGTAGTGACGTGGCCAAAGCCCTGC ACAACCTCCTCCGCTTCCTGGCCAGCAACAAGCCCCAGGAGATGGCCAATGCTTCCCTCACCCTGGAGAC ACCATTGACCTCCAAGAGGAGCACCACCGGCAAAACGTCTGGGGCTGTCTGGGCAGTGCCTCCCACTGCC CAGGGGGACCAGGTGCCACTGAAGGTGCTGCTGCCCCCGGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204537 representing NM_001025680
Red=Cloning site Green=Tags(s) MDNSTGTWEGCHVDSRVDHLFPPSLYIFVIGVGLPTNCLALWAAYRQVRQRNELGVYLMNLSIADLLYIC TLPLWVDYFLHHDNWIHGPGSCKLFGFIFYSNIYISIAFLCCISVDRYLAVAHPLRFARLRRVKTAVAVS SVVWATELGANSAPLFHDELFRDRYNHTFCFEKFPMERWVAWMNLYRVFVGFLFPWALMLLCYRGILRAV QSSVSTERQEKVKIKRLALSLIAIVLVCFAPYHALLLSRSAVYLGRPWDCGFEERVFSAYHSSLAFTSLN CVADPILYCLVNEGARSDVAKALHNLLRFLASNKPQEMANASLTLETPLTSKRSTTGKTSGAVWAVPPTA QGDQVPLKVLLPPAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001025680 |
ORF Size | 1095 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025680.1, NP_001020851.1 |
RefSeq Size | 1513 bp |
RefSeq ORF | 1098 bp |
Locus ID | 308408 |
UniProt ID | Q4KLH9 |
MW | 41.3 kDa |
Gene Summary | Proton-sensing receptor coupled to several G-proteins, including G(s), G(13) and G(q)/G(11) proteins, leading to cAMP production.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204537 | Gpr4 (untagged ORF) - Rat G protein-coupled receptor 4 (Gpr4), (10 ug) |
CNY 3,990.00 |
|
RR204537L3 | Lenti ORF clone of Gpr4 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 4 (Gpr4), (10 ug) |
CNY 6,080.00 |
|
RR204537L4 | Lenti ORF clone of Gpr4 (mGFP-tagged ORF) - Rat G protein-coupled receptor 4 (Gpr4), (10 ug) |
CNY 6,650.00 |