Ca5a (NM_019293) Rat Tagged ORF Clone
CAT#: RR204765
- TrueORF®
Car5a (Myc-DDK-tagged ORF) - Rat carbonic anhydrase 5a, mitochondrial (Car5a), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_019293" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Ca5; Car5a |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204765 representing NM_019293
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCAGAGCCAAGATGCTCGGGAGAGGCCCCTACAAGCCCTTAGCCATCCTCAGGCACATGGGACCTC TCTGTGCCACAAGGCCACAGCACTGGCGCTTCCAGCATTCCTACGCAGAGAAACACAGCAACTGTGCCCG GCACCCTCTCTGGACTGGCCCAGTGTCCTCACCGGGAGGCACCCAGCAGTCTCCCATTAATATCCAGTGG ACGGATAGTGTCTATGACCCGAAGCTGGCACCGCTCAGGGTCTCCTATGATGCTGCGTCCTGCAGATACC TCTGGAACACTGGTTACTTCTTCCAGGTGGAGTTTGACGATTCCTGTGAGGAGTCAGGGATCAGTGGTGG GCCTCTGGGAAACCACTACAGGCTGAAGCAGTTTCACTTCCACTGGGGAGCAACAGATGAATGGGGCTCT GAGCACATGGTGGACGGCCATGCCTACCCGGCTGAGCTCCATTTGGTTCACTGGAATTCCATGAAATATG AAAATTACAAGAAAGCCACCACGGGGGAGAATGGACTGGCGGTGATTGGAGTGTTTCTGAAGCTCGGGGC CCATCACGAGGCCCTGCAGAGGCTGGTGGACATCTTGCCGGAAGTAAGACACAAGGACACACAGGTGACC ATGGGGCCCTTTGACCCTTCTTGCCTGCTGCCTGCCTGCCGGGATTACTGGACCTACCCTGGCTCCCTCA CCACCCCACCACTGGCTGAGTCAGTCACCTGGATTGTGCACAAGATGCCCATTGAGGTGTCCCCGAGCCA GCTGTCCACATTCCGTACACTCTTGTTCTCCGGGCGAGGTGAGGACGAGGAGGTGATGGTGAACAACTTC CGCCCGCTCCAACCACTCAGGGGCCGCAACGTTCGCTCCTCCTTCCAGGTCCCCAGGGTGGGAACAAAGT CT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204765 representing NM_019293
Red=Cloning site Green=Tags(s) MLRAKMLGRGPYKPLAILRHMGPLCATRPQHWRFQHSYAEKHSNCARHPLWTGPVSSPGGTQQSPINIQW TDSVYDPKLAPLRVSYDAASCRYLWNTGYFFQVEFDDSCEESGISGGPLGNHYRLKQFHFHWGATDEWGS EHMVDGHAYPAELHLVHWNSMKYENYKKATTGENGLAVIGVFLKLGAHHEALQRLVDILPEVRHKDTQVT MGPFDPSCLLPACRDYWTYPGSLTTPPLAESVTWIVHKMPIEVSPSQLSTFRTLLFSGRGEDEEVMVNNF RPLQPLRGRNVRSSFQVPRVGTKS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019293 |
ORF Size | 912 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019293.3, NP_062166.1 |
RefSeq Size | 1260 bp |
RefSeq ORF | 915 bp |
Locus ID | 54233 |
UniProt ID | P43165 |
MW | 34.5 kDa |
Gene Summary | mitochondrial protein that may be involved in gluconeogenesis and ureagenesis [RGD, Feb 2006] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204765 | Car5a (untagged ORF) - Rat carbonic anhydrase 5a, mitochondrial (Car5a), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,990.00 |
|
RR204765L3 | Lenti ORF clone of Car5a (Myc-DDK-tagged ORF) - Rat carbonic anhydrase 5a, mitochondrial (Car5a), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
RR204765L4 | Lenti ORF clone of Car5a (mGFP-tagged ORF) - Rat carbonic anhydrase 5a, mitochondrial (Car5a), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |