Lias (NM_001012037) Rat Tagged ORF Clone
CAT#: RR204792
- TrueORF®
Lias (Myc-DDK-tagged ORF) - Rat lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein, (10 ug)
"NM_001012037" in other vectors (3)
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CNY 3,990.00
CNY 300.00
Specifications
Product Data | |
Type | Rat Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RR204792 representing NM_001012037
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTACGCTGCTGGGATGCAGCTCGCAGCCTGGGGTCCCGGATATTTGGAAGATATGCGTGCTCGG TTAGAGCATTAAGTTCTTTGCCAGATGAGAAAAAGAAATTTTTGCATAATGGGCCAGACCTTCAAGATTT TGTATCTGGTGATCTTGCAGACAAGAGTACCTGGGATGATTATAAAGGAAACTTAAAGCGCCAGAAAGGA GAAAGGTTAAGACTACCTCCGTGGTTAAAGACAAAGATACCCATGGGTAAAAATTACAATAAGCTGAAAA ATACGTTGCGGAATTTAAATCTCCACACAGTATGTGAGGAAGCCCGATGTCCAAACATCGGAGAGTGTTG GGGAGGTGGGGAATATGCCACCGCCACAGCCACGATCATGTTGATGGGGGACACATGCACAAGAGGTTGC AGATTTTGCTCTGTTAAGACGGCAAGAAATCCCCCTCCATTGGATCCCAGTGAGCCCGACAATACGGCCA GAGCAATCGCAGAGTGGGGTCTGGACTATGTTGTGCTGACGTCTGTGGACCGAGATGATGTGGTCGATGG GGGAGCTGAGCACATTGCCAAGACAGTGTCATGCTTGAAGGAAAGGAATCCAAAAATCCTCGTGGAATGC CTCACCCCCGACTTCCGAGGAGATCTGAGAGCTGTAGAAAAGGTGGCGCTGTCTGGACTAGATGTGTATG CACACAATGTAGAGACGGTTCCAGAATTACAGAGGAAAGTCCGTGATCCCCGGGCCAATTTTGACCAGTC TCTTCGTGTATTGAAACATGCCAAGGAGGTGCAGCCTGATGTTGTTTCCAAAACATCAATAATGCTGGGC CTCGGTGAGACAGACGAGCAGGTCTATGCCACGATGAAAGCTCTCCGTGCAGCCGATGTGGACTGTTTAA CTCTCGGGCAGTATATGCAGCCGACCAAGCGCCACCTTAAGGTTGAAGAGTACGTTACTCCTGAGAAGTT CAAATACTGGGAAGAAGTAGGAAATGAACTTGGATTTCACTACACTGCAAGTGGCCCTTTGGTGCGATCT TCATATAAAGCAGGTGAATTTTTCCTGAAAAATCTAGTGGCTAAAAGAAAAACAAAAGTTTCTAAAGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR204792 representing NM_001012037
Red=Cloning site Green=Tags(s) MALRCWDAARSLGSRIFGRYACSVRALSSLPDEKKKFLHNGPDLQDFVSGDLADKSTWDDYKGNLKRQKG ERLRLPPWLKTKIPMGKNYNKLKNTLRNLNLHTVCEEARCPNIGECWGGGEYATATATIMLMGDTCTRGC RFCSVKTARNPPPLDPSEPDNTARAIAEWGLDYVVLTSVDRDDVVDGGAEHIAKTVSCLKERNPKILVEC LTPDFRGDLRAVEKVALSGLDVYAHNVETVPELQRKVRDPRANFDQSLRVLKHAKEVQPDVVSKTSIMLG LGETDEQVYATMKALRAADVDCLTLGQYMQPTKRHLKVEEYVTPEKFKYWEEVGNELGFHYTASGPLVRS SYKAGEFFLKNLVAKRKTKVSKV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001012037 |
ORF Size | 1119 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001012037.1, NP_001012037.1 |
RefSeq Size | 1636 bp |
RefSeq ORF | 1122 bp |
Locus ID | 305348 |
UniProt ID | Q5XIH4 |
MW | 41.9 kDa |
Gene Summary | Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RN204792 | Lias (untagged ORF) - Rat lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 3,990.00 |
|
RR204792L3 | Lenti ORF clone of Lias (Myc-DDK-tagged ORF) - Rat lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,080.00 |
|
RR204792L4 | Lenti ORF clone of Lias (mGFP-tagged ORF) - Rat lipoic acid synthetase (Lias), nuclear gene encoding mitochondrial protein, (10 ug) |
CNY 6,650.00 |