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Ncstn (NM_174864) Rat Tagged ORF Clone
CAT＃: RR205108
- TrueORF®
Ncstn (Myc-DDK-tagged ORF) - Rat nicastrin (Ncstn), (10 ug)
"NM_174864" in other vectors (3)
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CNY 9,120.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Rat Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RR205108 representing NM_174864
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTACGGCTAGGGGCGGCTCTGGGCCTGACCCAGGAAGTCGGGGTCTCCTTCTTCTGTCTTTTTCCG TGGTACTAGCAGGATTGTGTGGGGGAAACTCAGTGGAGAGGAAGATCTACATTCCCTTAAATAAAACAGC TCCTTGTGTCCGCCTGCTCAATGCCACTCATCAGATTGGCTGCCAGTCTTCAATTAGTGGGGATACAGGG GTTATCCACGTAGTGGAGAAAGAAGACGACCTGAAATGGGTATTGACGGATGGCCCCAACCCCCCTTACA TGGTTCTGCTGGAGGGCAAGCTCTTTACCAGAGATATAATGGAAAAGCTGAAGGGGGAAACCAGTAGAAT CGCTGGTCTTGCCGTGACTCTAGCCAAGCCCAATTCAACGTCAAGCTTCTCTCCTAGCGTGCAGTGCCCA AATGATGGGTTTGGTATTTACTCCAACTCCTATGGGCCAGAGTTTGCTCACTGCAAAAAAACATTGTGGA ATGAACTGGGCAATGGCTTGGCTTATGACGACTTCAGTTTCCCCATCTTTCTTCTTGAAGATGAGAACGA AACCAAGGTCATCAAGCAGTGCTATCAAGATCACAACCTGGGTCAGAATGGCTCTGCACCGAGCTTCCCA CTGTGTGCTATGCAGCTCTTCTCACACATGCACGCCGTCATCAGCACCGCCACCTGCATGCGACGCAGCT TCATCCAGAGCACCTTCAGCATCAACCCAGAAATCGTCTGTGACCCCTTATCCGACTACAACGTATGGAG CATGCTTAAGCCTATAAATACGTCTGGGGGATTAGAACCTGATGTCAGGGTTGTGGTTGCAGCCACACGG CTGGATAGCCGGTCCTTCTTCTGGAATGTGGCCCCAGGGGCAGAAAGTGCCGTTGCCTCCTTTGTCACTC AGCTGGCCGCAGCTGAAGCTTTGCACAAGGCACCTGATGTGACCACCCTACCCCGAAATGTGATGTTTGT CTTCTTCCAGGGGGAAACTTTTGACTACATTGGCAGCTCAAGGATGGTCTATGACATGGAGAACGGCAAG TTTCCTGTGCGGCTAGAGAACATTGACTCCTTTGTGGAGCTGGGGCAGGTGGCCCTAAGGACGTCACTAG AACTCTGGATGCACACAGATCCTATGTCTCAGAAAAATGAATCTGTGAAGAACCAGGTGGAGGATCTTCT GGTCACTCTGGAGCAGAGTGGTGCTGATACCCCTCAAGTTGTCCTGAGCAGACTAGTCCAGTCCCAGGCC CTTCCACCATCATCTCTACAACGATTTCTTCGGGCTCGAAACATCTCTGGTGTGGTCTTGGCTGACCACT CTGGTTCCTTCCACAATCGGTATTACCAGAGCATTTATGACACTGCTGAGAACATTAACGTGACCTATCC TGAGTCGCAGAGCCCAGAAGAGGACCTCAACTTTGTAACAGACACTGCCAAGGCACTGGCAGATGTTGCC ACAGTGCTGGCACGAGCTTTGTACAAGCTTGCAGGAGGAACCAACTTCAACAACTCCATTCAGGCTGATC CCCAGACAGTTACACGTCTGCTCTATGGGTTCCTGGTCAGAGCTAACAACTCATGGTTTCAGTCTATCCT CAGACATGATCTGAGGTCCTATTTGGATGATGGGCCTCTTCAACACTACATCGCTGTCTCCAGCCCTACC AACACGACTTACGTTGTGCAGTATGCCTTGGCAAACCTGACTGGCAAGGTGACCAACCTCACCCAAGAGC AGTGCCAGGATCCAAGTAAAGTCCCAAATGAAAGCAAGGATTTGTATGAATACTCGTGGGTACAAGGCCC TTGGAATTCCAACAAGACAGAGCGGCTCCCACGGTGTGTGCGCTCCACAGTACGACTGGCTAGGGCCTTG TCCCCCGCCTTTGAGCTGAGTCAGTGGAGCTCCACAGAATACTCTACGTGGGCCGAGAGCCGCTGGAAAG ATATCCAAGCTCGGATATTCCTCATTGCCAGCAAAGAACTTGAGTTCATCACGCTGATCGTGGGCTTCAG CATCCTCGTCTTTTCTCTCATCGTCACTTATTGTATCAACGCCAAGGCCGATGTCCTTTTCGTTGCTCCC CGAGAGCCAGGAGCTGTGTCTTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RR205108 representing NM_174864
Red=Cloning site Green=Tags(s) MATARGGSGPDPGSRGLLLLSFSVVLAGLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTG VIHVVEKEDDLKWVLTDGPNPPYMVLLEGKLFTRDIMEKLKGETSRIAGLAVTLAKPNSTSSFSPSVQCP NDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYDDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFP LCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSGGLEPDVRVVVAATR LDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMENGK FPVRLENIDSFVELGQVALRTSLELWMHTDPMSQKNESVKNQVEDLLVTLEQSGADTPQVVLSRLVQSQA LPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPESQSPEEDLNFVTDTAKALADVA TVLARALYKLAGGTNFNNSIQADPQTVTRLLYGFLVRANNSWFQSILRHDLRSYLDDGPLQHYIAVSSPT NTTYVVQYALANLTGKVTNLTQEQCQDPSKVPNESKDLYEYSWVQGPWNSNKTERLPRCVRSTVRLARAL SPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSILVFSLIVTYCINAKADVLFVAP REPGAVSY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_174864 |
| ORF Size | 2124 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_174864.3, NP_777353.1 |
| RefSeq Size | 2915 bp |
| RefSeq ORF | 2127 bp |
| Locus ID | 289231 |
| UniProt ID | Q8CGU6 |
| MW | 78.4 kDa |
| Gene Summary | component of the gamma-secretase complex, which catalyzes the cleavage of amyloid precursor protein APP [RGD, Feb 2006] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RN205108 | Ncstn (untagged ORF) - Rat nicastrin (Ncstn), (10 ug) |
CNY 9,120.00 |
|
| RR205108L3 | Lenti ORF clone of Ncstn (Myc-DDK-tagged ORF) - Rat nicastrin (Ncstn), (10 ug) |
CNY 10,930.00 |
|
| RR205108L4 | Lenti ORF clone of Ncstn (mGFP-tagged ORF) - Rat nicastrin (Ncstn), (10 ug) |
CNY 11,970.00 |
